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Citations for PerkinElmer :
51 - 100 of 796 citations for Glycerol Kinase 1 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... incubated with 3% of H2O2 for 30 minutes at RT and blocked 1 hour RT with NEN blocking buffer prepared according to the manufacturer’s protocol (FP1012 PerkinElmer). AntiDIG antibody conjugated to HRP (1:1500 ...
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bioRxiv - Developmental Biology 2020Quote: ... incubated 1h in blocking buffer and then incubated overnight with an anti-DNP-POD antibody (1:1000, Perkin Elmer). The next day embryos were washed in PBS-Tween ...
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bioRxiv - Evolutionary Biology 2021Quote: ... samples were blocked in 0.5% blocking reagent (FP1020, PerkinElmer) at RT for 1 h ...
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bioRxiv - Cancer Biology 2024Quote: ... After at least 30-min block in blocking solution (Tris-NaCl-blocking buffer or TNB buffer, Perkin Elmer, with 0.1% Triton X-100), we incubated the tissues with rabbit primary antibodies (diluted in TNB ...
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bioRxiv - Biochemistry 2020Quote: ... peptides were tested for their ability to disrupt a second Alphascreen assay in which donor and acceptor beads were reacted with 50 nM concentrations of a peptide consisting of a biotinylation site and a His-tag peptide (PerkinElmer Life Sciences).
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Transient Nodal signalling in left precursors coordinates opposed asymmetries shaping the heart loopbioRxiv - Developmental Biology 2019Quote: ... Samples were blocked with the TSA Blocking Reagent (Perkin Elmer), incubated with the primary antibody Phospho-Smad2 (1/50 ...
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bioRxiv - Neuroscience 2023Quote: ... The slides were then blocked with blocking reagent (Perkin Elmer) and 0.5 % peroxidase block sequentially ...
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bioRxiv - Molecular Biology 2019Quote: ... DNA probes were 5’-phosphorylated with T4 polynucleotide kinase (PNK, TOYOBO) and [γ-32P] ATP (PerkinElmer). Northern blotting of tRNAs was performed using PerfectHyb (Toyobo ...
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bioRxiv - Biochemistry 2024Quote: ... 10% glycerol) were pipetted in triplicate onto black shallow 384-well microplates (OptiPlate-F, PerkinElmer) and incubated in the dark for 10 min at 25°C ...
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bioRxiv - Developmental Biology 2021Quote: ... Heat mediated antigen retrieval was used to process sections (Vector, H-3300) and then samples were blocked for 1 hour in blocking buffer at room temperature (PerkinElmer, FP1020). Primary antibodies diluted in blocking buffer were incubated with samples overnight at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... Reactions were started by adding 40 µl IR in alkyl maltoside micelles to 10 µl radioactive kinase reaction buffer (0.4 mM ATP, 1 µCi [γ-32P]-ATP (PerkinElmer, 10 mCi/mL, 25-50 cpm/mol), 1 mM MgCl2 ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were blocked with TSA blocking reagent (PerkinElmer, Waltham, MA, USA) and incubated with an anti-fluorescein horseradish peroxidase conjugate (1:500 ...
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bioRxiv - Genomics 2019Quote: ... followed by blocking with antibody diluent/blocker (Perkin Elmer, Waltham, MA) for 30 min ...
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bioRxiv - Developmental Biology 2019Quote: ... slides were blocked with TNB Blocking Buffer (Perkin Elmer, Waltham, MA) for 30 minutes at room temperature ...
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bioRxiv - Molecular Biology 2020Quote: mTOR kinase activity was monitored by LANCE Ultra time-resolved fluorescence resonance energy transfer (TR-FRET, PerkinElmer) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... Isolated cardiac myocytes were incubated with 0.1 mM [1,3-3H]glycerol (2 μCi/dish, Perkin Elmer) or 0.1 μM [1-14C]linoleic acid (2 μCi/dish ...
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bioRxiv - Biochemistry 2024Quote: ... 250 nM purified MBP-Naa80, 200 µM substrate peptide (Figure 1 and Supplemental Table S1, 50 µM 14C-Ac-CoA (Perkin Elmer) were mixed in acetylation buffer (50 mM Tris-HCl pH 8.5 ...
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bioRxiv - Cancer Biology 2020Quote: ... in PBS for 1h at RT and with TNB blocking buffer (PerkinElmer) for 30 min for EF5 staining ...
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bioRxiv - Neuroscience 2022Quote: ... Cryosections were permeabilized and blocked in TSA blocking reagent (Perkin Elmer FP1020). Primary antibodies used were anti-BK 1:100 (Antibodies Inc AB_224953) ...
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bioRxiv - Cancer Biology 2019Quote: ... in PBS followed by blocking with 0.5% PNB in PBS (Perkin Elmer). Primary antibodies were incubated overnight ...
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bioRxiv - Immunology 2022Quote: ... Non-specific antibody binding was avoided using Blocking Reagent™ (Perkin Elmer) for 30 min ...
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An endothelial SOX18-mevalonate pathway axis enables repurposing of statins for infantile hemangiomabioRxiv - Molecular Biology 2024Quote: ... Sections were subsequently blocked for 30 minutes in TNB Blocking Buffer (PerkinElmer) followed by incubation with an anti-human CD31 monoclonal antibody (1:30 ...
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bioRxiv - Biochemistry 2022Quote: ... 25 nM biotinylated and hexahistidine-tagged linker peptide (Bn-His6) (PerkinElmer) was added to the compounds ...
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bioRxiv - Physiology 2021Quote: ... immunoprecipitates were analyzed for kinase activity in presence of phosphatidylinositol (PI) and γ-32P-ATP (PerkinElmer, Santa Clara, CA) followed a published method 91 ...
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bioRxiv - Microbiology 2022Quote: ... Extracted crRNAs were end-labeled with T4 Polynucleotide Kinase in a reaction containing γ-[32P]-ATP (PerkinElmer, MA, USA), and resolved on an 8% Urea PAGE ...
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bioRxiv - Biophysics 2019Quote: ... pH 7.5/0-800 mM NaCl (equimolar substitution of NaCl with Tris/Mes)/20% glycerol/0.1 mM TCEP/0.1% DDM using 1.25 mg/mL copper His-tag PVT SPA beads (Perkin Elmer). Total binding was corrected for the non-proximity-based signal to determine the specific binding activity of each LeuT variant and data were normalized to the maximal binding observed for LeuT-WT that was assayed in parallel in all individual measurements ...
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bioRxiv - Neuroscience 2021Quote: ... The cryosections were permeabilized and blocked in TSA blocking reagent (Perkin Elmer FP1020). Primary antibodies anti-BK 1:100 Antibodies Inc AB_224953 ...
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bioRxiv - Neuroscience 2020Quote: ... and blocked by TN Blocking buffer (TSA Plus Fluorescence System; PerkinElmer, MA, USA). Primary antibodies were incubated for more than 18 hr at 4°C ...
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bioRxiv - Developmental Biology 2022Quote: ... 0.1% Tween20) and then blocked in TNT/block [0.5% blocking reagent (PerkinElmer FP1012)/TNT] for 1 hour at RT before ON incubation with anti-DIG (1:100 ...
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bioRxiv - Immunology 2022Quote: ... Unspecific antibody binding on sorted cells was blocked with TSA blocking reagent (PerkinElmer) for 30 minutes ...
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bioRxiv - Developmental Biology 2023Quote: ... followed by PBS washing and treated with TNB blocking reagent (Perkin Elmer, FP1012) for 1 hour at RT ...
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bioRxiv - Neuroscience 2024Quote: ... incubated in TNB (Blocking Reagent [Perkin Elmer; 0.05% in TN]; 30 min, RT), incubated with anti-DIG-POD antibody (Roche ...
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bioRxiv - Immunology 2021Quote: ... Peptide masses were initially predicted by ChemDraw Professional 15.0 (PerkinElmer, Massachusetts, USA) and compared to acquired masses after LC-MS measurements.
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bioRxiv - Microbiology 2021Quote: ... an equal volume of biotinylated SRC1 peptide/Europium-labeled streptavidin (Perkin Elmer) was added to biotynlated RORγ/APC-labeled strepavidin (Perkin Elmer) ...
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bioRxiv - Immunology 2022Quote: ... 0.2 µM kinase was incubated with 7 µM substrate and 0.2 mM ATP containing 0.45 mCi [32P]-γ-ATP/mL (Perkin Elmer) in kinase buffer (50 mM HEPES pH 7.6 ...
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bioRxiv - Neuroscience 2023Quote: ... 10 mg of purified GST-fusion protein (unedited Cpx7AI125,N130 or edited Cpx7AI125M,N130S) was used per reaction and incubated with 2,500 units of recombinant kinase and [32P]ATP (Perkin Elmer). Reaction products were separated by SDS-PAGE and gels were stained with Bio-Safe Coomassie Blue (Bio-Rad) ...
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bioRxiv - Cell Biology 2021Quote: ... [9,10(n)-3H]oleic acid (OA) (54.6 Ci/mmol) and [14C]glycerol (250 μCi/mmol) were from Perkin Elmer. Inhibitors of DGAT1 (PF-04620110) ...
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bioRxiv - Microbiology 2024Quote: ... The overnight cultures were then back diluted in 200 µl DSM media (supplemented with the indicated amount of glycerol) to OD600=0.1 in a 96-well (black wall, clear bottom) microplate (PerkinElmer). The plate was then incubated at 37 °C with continuous shaking (600 cpm ...
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bioRxiv - Microbiology 2021Quote: ... pre-incubated with blocking buffer (TNB) supplied in the TSA Plus kit (Perkin Elmer) for 30 min ...
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bioRxiv - Cancer Biology 2021Quote: ... Table 9) and the compounds added to plates using the then kinase compounds added by the Sciclone ALH3000 robot (Perkin Elmer). Organoids were imaged using the Cytation5 (BioTek ...
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bioRxiv - Molecular Biology 2023Quote: ... incubation with a 32P-labelled oligodeoxyribonucleotide probe complementary to the target RNAs (32P-labelled using T4 polynucleotide kinase and [g 32P]-ATP [Perkin Elmer]) overnight in 37°C in SES1 buffer ...
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bioRxiv - Microbiology 2020Quote: ... Uptake was measured at room temperature by mixing 100 µl of cells with 100 µl of transport buffer containing 8 µM of [14C] glycerol (0.125 µCi each in triplicates, PerkinElmer Inc.). Incorporation of radioactivity was determined as described above.
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bioRxiv - Microbiology 2024Quote: ... Glycoconjugate aliquots of 75 µL to 5 mL (3.5-5 mg/mL in 25% glycerol) were injected and passed over a C4 column (either Perkin Elmer Epic C4 column or alternatively Phenomenex Jupiter 10u C4 300Å 250−10 mm 10 micron) ...
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bioRxiv - Microbiology 2023Quote: ... Tissues were blocked in 3% hydrogen peroxide followed by TSA blocking buffer (Perkin Elmer #FP1012). Tissues were then incubated with rabbit Sera against MmuPV1 L1 (provided by Dr ...
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bioRxiv - Biochemistry 2020Quote: ... Peptide stocks in DMSO were spotted onto 384-well white OptiPlates (PerkinElmer Life Sciences) at concentration ranges of 1 nM to 100 M using a Labcyte Echo 555 Omics acoustic liquid handler (Labcyte ...
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bioRxiv - Bioengineering 2019Quote: ... Aliquots of the peptide were deposited on a “Golden Gate” diamond ATR (PerkinElmer, USA). PBS was used as a background spectrum ...
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bioRxiv - Cell Biology 2021Quote: ... Then it was mixed with reaction B which contain 5 μg of the GST-substrate in 5x Kinase buffer containing 2 μCi γ-32P-ATP (PerkinElmer, BLU002Z250UC) in 10-μl reactions ...
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bioRxiv - Biochemistry 2019Quote: ... The RNA 5’ phosphate was removed by quick dephosphorylation kit (New England biolabs) and phosphorylated by T4 polynucleotide kinase (New England biolabs) and [γ -32P]ATP (3000 Ci/mmol) (Perkin Elmer). The samples were loaded onto 10% polyacrylamide denaturing gel (8 M urea) ...
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bioRxiv - Neuroscience 2023Quote: ... produced in Sf9 cells, and ROCK1 (Carna Biosciences, Natick, MA; 5 nM) using the Lance-FRET kinase assay system (PerkinElmer, Waltham, MA). Other human recombinant PKCs (SignalChem Biotech ...
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bioRxiv - Microbiology 2020Quote: ... mounted with glycerol and analyzed in an Operetta high-content imaging system with a 40x objective and high numerical apertures (NA) (PerkinElmer, USA). The data was analyzed using the high-content image analysis software Harmony 5.1 (PerkinElmer ...