Labshake search
Citations for PerkinElmer :
151 - 200 of 205 citations for GC TEMPase 2x Master Mix II since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2020Quote: ... 200 μCi (7.4 MBq) [35S]-methionine/L-[35S] cysteine using EasyTag™ EXPRESS35S Protein Labeling Mix (PerkinElmer, Groningen, the Netherlands) was added to the medium ...
-
bioRxiv - Microbiology 2023Quote: ... cells were cultured in 6-well plates and metabolically labeled (Easytag Express 35S-Met-Cys protein labeling mix (Perkin Elmer)) with 0.2 mCi/ml for various times ...
-
bioRxiv - Cell Biology 2023Quote: ... On the next day transfer and mix of compounds into plates only containing cell culture media was performed with the Sciclone G3 Liquid Handling Workstation (PerkinElmer). This intermediate dilution step was included to avoid DMSO gradient effects on cell monolayers ...
-
bioRxiv - Genetics 2023Quote: ... and subsequently pulsed for 30 minutes in starvation medium supplemented with 200µCi/mL EasyTagTM EXPRESS 35S protein labeling mix (Perkin Elmer). Cells were chased in RPMI supplemented with 0.4 mM methionine for 0 ...
-
bioRxiv - Neuroscience 2021Quote: ... 100 µL of 2% ACN/0.1% TFA was added to the reaction mixture and the mixture was analyzed by mobility shift assay (LabChip EZ Reader II; PerkinElmer, U.S.A.). The kinase activity is the percentage of phosphorylated peptide signal over the total substrate peptide signal ...
-
P2RX7 inhibition reduces breast cancer induced osteolytic lesions - implications for bone metastasisbioRxiv - Cancer Biology 2022Quote: ... The tumour growth was monitored by Vernier calliper measurements and bioluminescence–based in vivo imaging using IVIS Lumina II In vivo Imaging System and quantified by Living Image® software (version 4.5.4, PerkinElmer, Beaconsfield, UK) twice a week ...
-
bioRxiv - Immunology 2021Quote: ... dissolved in PBS (150 μg/mL) 10min before analysis performed using the IVIS® Lumina II platform (PerkinElmer, Waltham, MA, USA). Photons emitted from luciferase-expressing cells in selected regions of interest (ROI ...
-
bioRxiv - Cancer Biology 2020Quote: ... Signals from luciferase-tagged cells were monitored at day 0 after injection and weekly by in vivo imaging using IVIS Lumina II (Perkin Elmer) for 5 weeks ...
-
bioRxiv - Plant Biology 2024Quote: ... was acquired and analyzed using IVIS Lumina II equipped with an XFOV-24 lens and Living Image 4.3.1 software (PerkinElmer, Waltham, MA, USA). Optical fluorescence is presented as radiance intensity (photons s−1 cm−2 steradian−1).
-
bioRxiv - Cancer Biology 2023Quote: ... bioluminescence imaging was performed once a week until the end of the study using an IVIS-Lumina II (Perkin Elmer, France). Each mouse was injected intraperitoneally with 100mg/kg luciferin potassium salt (Promega ...
-
bioRxiv - Molecular Biology 2021Quote: ... LPS-stimulated plasmablasts were starved for methionine and cysteine for 60 minutes and pulse labeled for 15 minutes with 55 µCi/1×106 cells of Express 35S protein labeling mix (Perkin Elmer). Samples were then diluted 10x with cell culture media containing 5mM unlabeled methionine and cysteine ...
-
bioRxiv - Cell Biology 2019Quote: ... 3 OD600 units of early log phase cells were labeled with 80 mCi of L-[35S]-methionine/cysteine mix (Perkin Elmer). Immunoprecipitated proteins were separated on SDS-PAGE and exposed to phosphor screens ...
-
bioRxiv - Biochemistry 2021Quote: ... and mRNA was translated for 1 hour at 30 °C in presence of 10 µCi/µL EasytagTM 35S Express Protein Labeling Mix (Perkin Elmer). The mixtures were centrifuged for 2 min at 10,000 x g at 4 °C after inhibiting protein synthesis with 1 mM cycloheximide and translocated CFTR was retrieved from the pellet fraction ...
-
bioRxiv - Cell Biology 2020Quote: ... supplemented with 10% dialyzed fetal calf serum and then metabolically labelled for 10 min with 55 μCi/ml 35S-methionine/cysteine protein labeling mix (Perkin Elmer). Cells were rinsed immediately with PBS twice and solubilized with 1% Triton X-100 in solubilization buffer (50 mM HEPES-KOH ...
-
bioRxiv - Molecular Biology 2020Quote: Nascent proteins in U-2 OS cells expressing GFP-G3BP1 were labeled with 35S-met and -cys (EXPRE35S35S Protein Labeling Mix, Perkin Elmer) for 30 minutes in the presence of DMSO (0.1%) ...
-
bioRxiv - Biochemistry 2020Quote: ... with undigested pOTB7/PDIP38 as template and 11 μCi of [35S]Met/CysEXPRE35S35S protein labelling mix (specific activity of >1000 Ci/mmol) from Perkin Elmer. Protein Assay (Bio-Rad ...
-
bioRxiv - Biochemistry 2021Quote: ... cells were starved for cysteine and methionine for 15-30 min and pulse labeled for 5 min with 55 µCi/ 35-mm dish of Easytag express 35S protein labeling mix (Perkin Elmer). Where indicated (+DTT) ...
-
bioRxiv - Biochemistry 2022Quote: ... Cells were pulse labeled for 15 minutes with 132 μCi/6 cm dish with EasyTag Express 35S Protein Labeling Mix (Perkin Elmer). Radiolabeling was stopped by adding excess ...
-
bioRxiv - Neuroscience 2023Quote: ... 50 μl of media from each sample and 50 μl of the enzyme and substrate mix was pipetted in a well of the imaging plate (PerkinElmer, 6055300). The plate was briefly mixed and incubated for 1 h at RT avoiding exposure to light ...
-
bioRxiv - Cancer Biology 2023Quote: ... then each well was incubated for 30 min at 37 °C with 5 μl/well EXPRE35S35S Protein Labeling Mix (PerkinElmer NEG072002MC), after which cells were lysed in RIPA lysis buffer (50 mM Tris-HCl pH 7.5 ...
-
bioRxiv - Cell Biology 2023Quote: ... and 1% antibiotic/antimycotic at 37°C for 30 min and then labeled with Express [35S] Labeling Mix (PerkinElmer, NEG072-007) for 15 min ...
-
The conserved membrane-proximal domain of Sbh1/ Sec61β guides signal peptides into the Sec61 channelbioRxiv - Biochemistry 2024Quote: ... Proteins were labelled for 5 mins (for soluble proteins) or 15 mins (for membrane proteins) with 30 µCi of Express Protein Labelling Mix (Perkin Elmer) per sample at their restrictive temperature ...
-
bioRxiv - Molecular Biology 2019Quote: ... The radioactivity present in Poly-A+ and total RNA fractions was measured using Liquid Scintillation (Optiphase Hisafe II; Perkin Elmer, 1200-436) and the Liquid Scintillation Analyzer (Perkin Elmer ...
-
bioRxiv - Bioengineering 2019Quote: ... bioluminescence imaging (BLI) was performed weekly with a commercial IVIS Lumina II imaging system (Caliper Life Sciences, now Perkin Elmer, Waltham, MA). Mice were injected i.p ...
-
bioRxiv - Genetics 2023Quote: ... Total RNA was Total RNA is normalized to 12.5 ng/μL in a total volume of 47 μL on the Perkin Elmer Janus Workstation (Perkin Elmer, Janus II). Poly-A selection and cDNA synthesis were performed using the TruSeq Stranded mRNA kit as outlined by the manufacturer (Illumina RS-122-2103) ...
-
bioRxiv - Cancer Biology 2024Quote: ... and imaged with a PerkinElmer Pannoramic 250 Flash II digital whole-slide scanner using a 20x 0.8NA Plan-Apochromat objective (PerkinElmer, Hopkinton, MA, USA). Images of individual fields of view were imported into ImageJ or VisioPharm for analysis ...
-
bioRxiv - Cell Biology 2020Quote: ... Cells were pulse labeled for 1 hr with 120 μCi of EasyTag Express35S Protein Labeling Mix [35S]-Cys/Met (PerkinElmer; Waltham, MA). Immediately after the radioactive pulse ...
-
bioRxiv - Cell Biology 2021Quote: ... 15 ul probe mix was added to the corresponding wells of 384-well plate prepared using a JANUS liquid handler workstation (PerkinElmer, Waltham, MA). Probes were denatured at 85°C for 7 min ...
-
bioRxiv - Cell Biology 2022Quote: ... HEK293T cells transfected with FTTTRA25T and were labeled for 30 min with EasyTag Express [35S] Protein Labeling Mix (0.1 mCi/mL; Perkin Elmer CAT# NEG772014MC) in DMEM lacking both Cys and Met (Gibco CAT# 21013024 ...
-
bioRxiv - Immunology 2021Quote: ... HRP enzyme activity was determined after the addition of a 1:1 mix of Western Lightning oxidizing and luminol reagents (PerkinElmer Life Sciences). Light emission was measured with an LB942 TriStar luminometer (Berthold Technologies) ...
-
bioRxiv - Microbiology 2021Quote: ... HRP enzyme activity was determined after the addition of a 1:1 mix of Western Lightning oxidizing and luminol reagents (PerkinElmer Life Sciences). Light emission was measured with an LB 942 TriStar luminometer (Berthold Technologies) ...
-
bioRxiv - Cell Biology 2022Quote: Confluent 6-well dishes of FRT cells (approximately 1×106/well) were metabolically labeled in DMEM depleted of methionine and cysteine and supplemented with EasyTag 35S Protein Labeling Mix (Perkin Elmer, NEG772007MC), glutamine ...
-
bioRxiv - Neuroscience 2021Quote: ... Biotin-labeled second-round aRNA was generated with an NTP-mix containing Biotin-11-CTP and Biotin-16-UTP (PerkinElmer, Boston, MA) at 2 mM ...
-
bioRxiv - Plant Biology 2020Quote: ... concentrations were measured by Inductively Coupled Plasma Mass Spectrometry using Dynamic Reaction Cell technology (ICP-MS ELAN DRC II, PerkinElmer Inc., MA, USA) (Benedicto et al. ...
-
bioRxiv - Plant Biology 2020Quote: ... and a 2 mg subsample was analyzed for carbon and nitrogen content using a CHN elemental analyzer (2400 CHNS/O Series II, PerkinElmer, Waltham, MA, USA). Each 30 cm of the entire root system ...
-
bioRxiv - Microbiology 2020Quote: ... was visualized and quantified by measuring the luciferase activity in the liver at 44 hours after the challenge with sporozoites using the IVIS Lumina II Imaging System (Perkin Elmer Life Sciences). Before imaging ...
-
bioRxiv - Plant Biology 2023Quote: ... the HPLC system was coupled to a Ramona 2000 radioactivity flow detector (Raytest, Straubenhardt, Germany) with on-line addition of the scintillation cocktail Flo-Scint II (Perkin Elmer, MA, USA) at a volumetric ratio of 3:1.
-
bioRxiv - Microbiology 2021Quote: ... HRP enzyme activity was determined after the addition of a 1:1 mix of Western Lightning oxidizing and luminol reagents (Perkin Elmer Life Sciences). Light emission was measured with a LB942 TriStar luminomete (Berthold Technologies) ...
-
bioRxiv - Cancer Biology 2020Quote: ... containing 10% BSA and 3 µl [35S] EasyTag Express protein labelling mix (7 mCi, 259 MBq, 1175 Ci/mmol in 632 ml; Perkin Elmer, Espoo, Finland). After 2 h of incubation at 37 °C cells were washed twice with PBS ...
-
bioRxiv - Microbiology 2020Quote: ... HRP enzyme activity was determined after the addition of a 1:1 mix of Western Lightning oxidizing and luminol reagents (Perkin Elmer Life Sciences). Light emission was measured with a LB941 TriStar luminometer (Berthold Technologies) ...
-
bioRxiv - Immunology 2021Quote: ... HRP enzyme activity was determined after the addition of a 1:1 mix of Western Lightning oxidizing and luminol reagents (Perkin Elmer Life Sciences). Light emission was measured with a LB941 TriStar luminometer (Berthold Technologies) ...
-
bioRxiv - Immunology 2021Quote: ... HRP enzyme activity was determined after the addition of a 1:1 mix of Western Lightning oxidizing and luminol reagents (Perkin Elmer Life Sciences). Light emission was measured with a LB942 TriStar luminometer (Berthold Technologies) ...
-
bioRxiv - Microbiology 2021Quote: ... HRP enzyme activity was determined after the addition of a 1:1 mix of Western Lightning oxidizing and luminol reagents (Perkin Elmer Life Sciences). Light emission was measured with a LB942 TriStar luminometer (Berthold Technologies) ...
-
bioRxiv - Molecular Biology 2021Quote: ... HRP enzyme activity was determined after addition of 35 µl per well of a 1:1 mix of Western Lightning oxidizing and luminal reagents (Perkin Elmer Life Sciences) supplemented with 150 mM NaCl ...
-
bioRxiv - Immunology 2021Quote: ... HRP enzyme activity was determined after the addition of a 1:1 mix of Western Lightning oxidizing and luminol reagents (Perkin Elmer Life Sciences). Light emission was measured with a LB941 TriStar luminometer (Berthold Technologies) ...
-
bioRxiv - Immunology 2021Quote: ... HRP enzyme activity was determined after the addition of a 1:1 mix of Western Lightning oxidizing and luminol reagents (Perkin Elmer Life Sciences). Light emission was measured with a LB941 TriStar luminometer (Berthold Technologies) ...
-
bioRxiv - Microbiology 2022Quote: ... HRP enzyme activity was determined after the addition of 40 μL of a 1:1 mix of Western Lightning oxidizing and luminol reagents (Perkin Elmer Life Sciences). Light emission was measured with a LB942 TriStar luminometer (Berthold Technologies) ...
-
bioRxiv - Neuroscience 2023Quote: ... Reactions were initiated by adding 2.5 µM ATP at 27°C and were terminated after 60 min with 10 µL of 2.5X Stop Solution/Detection Mix containing 20mM EDTA and 4nM LANCE Ultra Europium-anti-phospho-PKC (Ala25Ser) peptide antibody (Perkin Elmer, Waltham, MA). After incubation at 27°C for another 60 min ...
-
bioRxiv - Genomics 2024Quote: ... size-selected sample was reamplified with 25 μL of NEXTFLEX PCR Master Mix 2.0 and 2 μL of 1:10 diluted NEXTFLEX Primer Mix 2.0 as described in the NEXTFLEX Cell free DNA-Seq Kit (V2) (Perkin Elmer, Pittsburgh, Pennsylvania, USA), step C PCR amplification ...
-
bioRxiv - Microbiology 2022Quote: ... while trace metals and metalloids by inductively coupled plasma mass-spectrometry (ICP-MS) using an ELAN DRC II spectrometer (Perkin Elmer, Waltham, MA, United States). Additional measurements were done for the aqueous fraction of the PMV4 sample ...