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Citations for PerkinElmer :
101 - 130 of 130 citations for Factor B Bb Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... 2.7 mM KCl with 0.2% Triton X-100 and then blocked by shaking for 60 min in TSA Blocking Reagent (Perkin Elmer). Sections were left shaking for 1-3 nights at 4°C in primary antibodies ...
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bioRxiv - Molecular Biology 2019Quote: ... Micrographs were captured with a digital camera mounted on a Leica DM6000 B upright microscope with either 10X or 40X objectives using Volocity Imaging software (version 6.3.0 PerkinElmer).
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Protein-bound [3H]ryanodine was separated by filtration through polyethyleneimine-treated glass filters (Filtermat B; PerkinElmer, Waltham, MA) using a Micro 96 Cell Harvester (Skatron Instruments ...
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bioRxiv - Biochemistry 2021Quote: In vitro peptide-based Aur A assays were carried out using a Caliper LapChip EZ Reader platform (Perkin Elmer), which monitors real-time phosphorylation-induced changes in the mobility of a fluorescently labelled Kemptide peptide substrate (5’-FAM-LRRASLG-CONH2 ...
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bioRxiv - Developmental Biology 2021Quote: ... Heat mediated antigen retrieval was used to process sections (Vector, H-3300) and then samples were blocked for 1 hour in blocking buffer at room temperature (PerkinElmer, FP1020). Primary antibodies diluted in blocking buffer were incubated with samples overnight at 4°C ...
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bioRxiv - Neuroscience 2019Quote: ... Sections were rinsed 3 times for 2 min in PBS-Tx and incubated in tyramide signal amplification (TSA) blocking reagent (PerkinElmer, FP1012) in PBS-Tx (TSA-block ...
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bioRxiv - Neuroscience 2021Quote: ... Then 5 µl of each diluted cell lysate was mixed with 15 µl of cell lysis buffer containing 0.33 mM ATP and 5 µM ProfilerPro Kinase Peptide Substrate 11 5-FAM-KKLNRTLSVA-COOH (PerkinElmer, U.S.A.) in the presence or absence of 0.66 µM CaM (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2020Quote: ... A LANCE Ultra TR-FRET kinase assay was used for measurements of enzyme activities that included an-Ulight labeled (Thr-1342) peptide substrate (catalog #TRF0130-M, PerkinElmer) and a Europium-labeled anti-phospho peptide antibody (catalog # TRF0218-M) ...
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bioRxiv - Biochemistry 2020Quote: ... was incubated with 5 μL of biotinylated peptides at 100 nM for 30 min at room temperature in the epigenetics assay buffer (PerkinElmer AL008 ...
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bioRxiv - Neuroscience 2022Quote: ... were challenged with synthetic peptides reconstituted in DMEM/BSA medium and luminescence was measured on a MicroBeta LumiJet luminometer (PerkinElmer). Luminescence was measured for 30 s at a wavelength of 469 nm ...
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bioRxiv - Biochemistry 2020Quote: ... the sections were washed and blocked with TN with 0.5% (w/v) Blocking Reagent TSA fluorescein system (Perkin Elmer, Waltham, MA, USA) for 30 minutes ...
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bioRxiv - Cancer Biology 2021Quote: ... the PFA-fixed sections were blocked and permeabilized in TNBT (0.1 M Tris, pH 7.4, 150 mM NaCl, 0.5% blocking reagent from Perkin Elmer, 0.5% Triton X-100) for 4h at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... or 3.3 mM (Figure 5-figure supplement 1 and 8) ATP and 5 µM ProfilerPro Kinase Peptide Substrate 11 5-FAM-KKLNRTLSVA-COOH (PerkinElmer, U.S.A.), in the presence or absence of 0.66 µM CaM (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... Radioactive counts from the media that remain soluble after TCA precipitation (peptide fragments) and pellets from the cells (total non-degraded cell proteins) were measured in a TopCount radioactivity detector (Perkin Elmer). A “protein degradation index” was calculated as the ratio between the counts detected in the soluble media and the counts detected in the pellet cell fraction (82).
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bioRxiv - Cell Biology 2019Quote: ... GST-Aq-NF-κB or GST-Aq-NF-κB-ALA C-terminal peptides and 5 μCi [γ-32P] ATP (Perkin Elmer) in kinase reaction buffer (25 mM Tris-HCl ...
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bioRxiv - Cancer Biology 2019Quote: ... Standard Fmoc-peptide synthesis was used to synthesize peptides on the amino-functionalized solid support by custom modified JANUS liquid handling stations (Perkin Elmer). The binding of antibody to each of the synthesized peptides was tested in a pepscan-based ELISA ...
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bioRxiv - Biophysics 2019Quote: AlphaScreen-based peptide recruitments assays were performed using streptavidin coated donor beads and anti-His-tag antibody coated AlphaLisa acceptor beads (Perkin Elmer). The assays were very sensitive to the distance of the N-terminus to the LXXLL NR-box motif and the use of a flexible Ahx linker ...
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bioRxiv - Biochemistry 2021Quote: ... and the level of 32P-transfer to the SAMS peptide was determined using a Tri-Carb 4810TR liquid scintillation counter (Perkin Elmer).
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bioRxiv - Biochemistry 2023Quote: ... 5 μL of 40 μM SFK peptide was added to each well and raw fluorescence units were measured immediately on an Envision fluorometer (Perkin Elmer) with an excitation wavelength of 344 nm and an emission wavelength of 405 nm in real time for the first 15 min at 15 s intervals ...
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bioRxiv - Biochemistry 2024Quote: ... 250 nM purified MBP-Naa80, 200 µM substrate peptide (Figure 1 and Supplemental Table S1, 50 µM 14C-Ac-CoA (Perkin Elmer) were mixed in acetylation buffer (50 mM Tris-HCl pH 8.5 ...
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bioRxiv - Developmental Biology 2021Quote: ... where reactions were incubated with 150 nM of the gel-filtration purified WT mouse Naa10 or Naa12 in a 30 µl reaction volume containing each 250 µM substrate peptide and radiolabeled [14C]acetyl-CoA (4 mCi/mmol; PerkinElmer Life Sciences) for 12 min (Naa12 ...
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bioRxiv - Cancer Biology 2023Quote: ... The phospho-peptide product was measured by time-resolved fluorescence resonance energy transfer (TR FRET) between the terbium of the antibody and fluorescein of the phospho-peptide using an EnVision plate reader (PerkinElmer, Waltham, MA) equipped to detect the TR-FRET signal (excitation 320 nm ...
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bioRxiv - Bioengineering 2022Quote: ... PEG-maleimide was reacted with 1 mol equivalence of calpain substrate peptide and 1 mol equivalence of L-cysteine-functionalized VivoTag S-750 (PerkinElmer, Boston, MA), followed by quenching with an excess of L-cysteine ...
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bioRxiv - Genomics 2022Quote: ... Total RNA was extracted from the female Epstein-Barr virus transformed B-lymphocyte cell line GM24143 using the Chemagic™ RNA TissuelO Kit H96 (PerkinElmer) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: LH levels in the medium were measured by means of a time-resolved fluorometric immunoassay with rat LH assay kits (National Hormone and Peptide Program, NHPP) and the Delfia system (Perkin Elmer, Waltham, MA) as reported previously (Kawaminami et al. ...
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bioRxiv - Neuroscience 2023Quote: ... Reactions were initiated by adding 2.5 µM ATP at 27°C and were terminated after 60 min with 10 µL of 2.5X Stop Solution/Detection Mix containing 20mM EDTA and 4nM LANCE Ultra Europium-anti-phospho-PKC (Ala25Ser) peptide antibody (Perkin Elmer, Waltham, MA). After incubation at 27°C for another 60 min ...
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bioRxiv - Cell Biology 2019Quote: ... The library was diluted to a 5 μM stock with 1x siRNA buffer (GE Dharmacon, B-002000-UB-100) and arrayed onto black 384-well PerkinElmer Cell Carrier plates (Perkin Elmer, 6007550) using the Echo Liquid Handler (Labcyte) ...
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bioRxiv - Cell Biology 2021Quote: ... Then it was mixed with reaction B which contain 5 μg of the GST-substrate in 5x Kinase buffer containing 2 μCi γ-32P-ATP (PerkinElmer, BLU002Z250UC) in 10-μl reactions ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The membrane mixture was then filtered over a 0.3% PEI pre-treated GF-B/C plate (#6005174, Perkin Elmer, Waltham, MA, USA) with a cell harvester system ...
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bioRxiv - Neuroscience 2022Quote: ... resulting from reduction of NADP+ to NADPH that was caused by glutamate oxidation by GDH was measured in a spectrometer (Luminescence Spectrometer LS 50 B, Perkin Elmer, Waltham, USA) for 600 or 700 seconds ...