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Citations for PerkinElmer :
251 - 300 of 824 citations for EnzyFluo ERK Phosphorylation Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2019Quote: ... For both assays, the released fluorescence (Ex: 380 nm, Em: 460 nM) was measured using an EnVision (Perkin Elmer) at a certain time.
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bioRxiv - Pathology 2021Quote: ... Cell viability was measured 72 hours after treatment using an ATPlite 1step detection assay (#6016739, PerkinElmer, Inc., Waltham, MA). To assess synergy between OTX008 and the PI3K inhibitors ...
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bioRxiv - Microbiology 2020Quote: ... luciferase activity was measured using the Britelite plus reporter gene assay system and Wallac BetaMax plate reader (Perkin Elmer).
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bioRxiv - Biochemistry 2021Quote: In vitro peptide-based Aur A assays were carried out using a Caliper LapChip EZ Reader platform (Perkin Elmer), which monitors real-time phosphorylation-induced changes in the mobility of a fluorescently labelled Kemptide peptide substrate (5’-FAM-LRRASLG-CONH2 ...
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bioRxiv - Immunology 2022Quote: ... ISH was performed using the RNAScope Multiplex Fluorescent V2 Assay (Advanced Cell Diagnostics (ACD)) with TSA Plus fluorescein (PerkinElmer) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2022Quote: ... 60-100 µg protein was incubated with 100 μl TG substrate (25 nmol triolein/assay and 40,000 cpm/nmol 14C-triolein; PerkinElmer) and 35.5 μg mixed micelles of phosphatidylcholine and phosphatidylinositol (3:1 ...
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bioRxiv - Cancer Biology 2023Quote: ... The promoter activity for each cell line was determined using Steadylite Plus reporter Gene Assay System (PerkinElmer Cat # 6066751) according to manufactural manual ...
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bioRxiv - Cancer Biology 2024Quote: ... viability was measured using the ATPlite 1step Luminescence Assay System according to the manufacturer’s protocol (Perkin Elmer, MA, USA) and the luminescence signals per well were detected with Envision Xcite 2104 plate reader with ultrasensitive luminescence detector (revvity ...
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bioRxiv - Neuroscience 2021Quote: ... The sections were then incubated with Biotinyl Tyramide Amplification kits (TSA, TSA plus biotin kit, PerkinElmer), following the manufacture’s protocol ...
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bioRxiv - Cell Biology 2019Quote: ... using the LANCE cAMP Kit (PerkinElmer) with a high sensitivity method with 12µL per point lysate and total detection volume 24µL using 2× concentrated detection mix ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Lance cAMP detection kit (PerkinElmer, AD0262) Alphascreen Surefire ERK1/2 (Thr202/Tyr204 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The pERK AlphaScreen kit (Perkin Elmer) was used for measuring pERK activity ...
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bioRxiv - Molecular Biology 2020Quote: ... and ECL kit (Perkin elmer #NEL104) was used before exposing the film for various durations (1 ...
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bioRxiv - Cancer Biology 2023Quote: ... The Opal 7 manual kit (PerkinElmer) was used according to manufacturer instructions ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... AlphaScreen TruHits Kit (PerkinElmer, cat # 6760627D) was used according to manufacturer instructions with minor modifications ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... AlphaLISA TruHits Kit (PerkinElmer, cat # AL900) was used according to manufacturer instructions ...
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bioRxiv - Biochemistry 2020Quote: ... The cell content was collected and used for IP1 assay (the homogeneous time-resolved fluorescence IP1 assay was used to measure intracellular IP1 accumulation according to the manufacturer’s directions (Cisbio Bioassays, Perkin Elmer). In brief ...
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bioRxiv - Cancer Biology 2021Quote: ... we used initially the RNAscope Multiplex Fluorescent Assay version 1 with the fluorophores Alexa 488 and Atto 550 and in later experiments the RNAscope Multiplex Fluorescent Assay version 2 with the fluorophores Opal 570 and Opal 650 (PerkinElmer) according the manufacturer’s protocol for multiplex fluorescent in situ hybridization kit ...
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bioRxiv - Cell Biology 2021Quote: ... The AlphaLISA™ assay was in 20 μL reactions (requiring only 2 μL cell culture media sample) in 384-well plate (6007290, PerkinElmer) following standard two-step no-wash protocol without any modifications ...
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bioRxiv - Microbiology 2022Quote: ... Plates were incubated for 48 h and the degree of viral entry was determined by luminescence using the neolite reporter gene assay system (PerkinElmer). IC50 values were determined by fitting dose-response curves with four-parameter logistic regression in GraphPad Prism 8 software.
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bioRxiv - Microbiology 2021Quote: ... coli TMPK catalysis was measured in the direction of ATP synthesis using the ATPlite luminescence assay (PerkinElmer cat. no. #6016739) in white 384-well plates (Corning cat ...
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bioRxiv - Microbiology 2019Quote: ... were infected with viral particles of Makona GP-A82 or V82 pseudoviruses in a 48 well plate using the same amount of p24 antigen quantified by AlphaLISA (AL291C:500 assay; PerkinElmer). After 48 h of incubation cells were washed twice with PBS and lysed for luciferase assay ...
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bioRxiv - Cell Biology 2019Quote: GBM2 cells were plated at 4000 cells/well and exposed to epigenetic compounds (Table 2) at 10 µM for 1 day in 384-well optical bottom assay plates (PerkinElmer). Negative control was DMSO (0.1%) ...
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bioRxiv - Cell Biology 2019Quote: GBM2 cells were plated at 2000 cells/well and exposed to Prestwick compounds (3 µM; Table 6) for 3 days in 384-well optical bottom assay plates (PerkinElmer). Cells were then fixed and stained with rabbit polyclonal anti-H3K27ac and mouse monoclonal anti-H3K27me3 antibodies followed by AlexaFluor-488- or AlexaFluor-555-conjugated secondary antibodies ...
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bioRxiv - Molecular Biology 2020Quote: ... The signals of time-resolved fluorescence resonance energy transfer (TR-FRET) assay were measured in an Envision plate reader (PerkinElmer) with a TR-FRET mode (Ex=320 ...
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bioRxiv - Biochemistry 2020Quote: ... was incubated with 5 μL of biotinylated peptides at 100 nM for 30 min at room temperature in the epigenetics assay buffer (PerkinElmer AL008 ...
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bioRxiv - Physiology 2020Quote: ... as per manufacturer’s instructions and loaded onto the DNA 5K/RNA/Charge Variant Assay LabChip (Perkin Elmer, Cat. No. 760435) to be read using a LabChip GX Touch Nucleic Acid Analyser (Perkin Elmer ...
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bioRxiv - Immunology 2020Quote: ... Plates were incubated for 48 hours and levels of viral infection were determined by luminescence using the neolite reporter gene assay system (PerkinElmer). Virus alone was used as a control and data was normalized to the control.
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bioRxiv - Microbiology 2021Quote: ... duplicate twofold serial dilutions of various compounds were prepared in assay medium in sterile 96-well black opaque plates (CulturPlate-96 Black, PerkinElmer). To allow for synergy testing ...
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bioRxiv - Immunology 2022Quote: ... using the RNA Assay and DNA 5K/RNA/CZE HT Chip on a LabChip GX Touch HT Nucleic Acid Analyzer (PerkinElmer). TruSeq Stranded mRNA Library Prep kit (Illumina ...
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bioRxiv - Microbiology 2022Quote: ... 48 hpi liver-stage cultures were washed with PBS and lysed in Nano-Glo Luciferase Assay buffer containing Nano-Glo Luciferase Assay substrate diluted 1:200 and luminescence was measured after 3 minutes on an IVIS Specturum CT (PerkinElmer). To monitor merosome detachment at 65 hpi ...
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bioRxiv - Systems Biology 2022Quote: ... All ELISA and colorimetric assays were performed according to manufacturer’s instructions in combination with a microplate reader (VICTOR3 Multilabel Plate Reader, Perkin Elmer).
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bioRxiv - Microbiology 2023Quote: ... The tissue lysates were mixed with an equal volume of Nano-GLO Luciferase Assay Reagent (Nanolight) and luciferase activity was quantified on an Envision 2103 plate reader (PerkinElmer). Decoy concentration and half-life were determined based on a standard curve derived from the mixture of serial diluted ACE2.1mb-nLuc proteins and tissue lysate from wild-type ...
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bioRxiv - Neuroscience 2022Quote: ... then incubated for 30 min at 25 °C with shaking at 400 RPM with 9 ng/well of DELFIA Eu-N1 anti-mouse antibody in DELFIA assay buffer (PerkinElmer), washed with 3 × 100 μl of PBST before enhancing with 80 μl of DELFIA Enhancement Solution (PerkinElmer) ...
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bioRxiv - Immunology 2022Quote: ... and interleukin 10 (IL-10) were quantified in the supernatant from the PBMCs using an AlphaLISA assay (PerkinElmer, Waltham, MA). AlphaPlates containing 2 µL of supernatant were thawed at 25°C for 15 minutes ...
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bioRxiv - Biochemistry 2023Quote: ... The assay was performed according to the manufacturer’s instructions and experiments described in (26) in white-opaque OptiPlate™-384 (PerkinElmer). Experiments to determine Km values were performed with increasing substrate concentrations ...
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bioRxiv - Molecular Biology 2023Quote: ... The average fragment size of the final libraries was determined using DNA 1K/12K/Hi Sensitivity Assay LabChip (760517, PerkinElmer)) and DNA High Sensitivity Reagent Kit (CLS760672 ...
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bioRxiv - Biochemistry 2023Quote: ... Reactions were initiated by adding an equal volume of assay buffer containing 1 μM [35S]GTPγS (~50 cpm/fmol, Perkin Elmer) at 30 °C ...
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bioRxiv - Immunology 2023Quote: ... After 10min of incubation at 37°C supernatants were collected and concentration of ATP/sample was measured using ATPlite Luminescence Assay System (PerkinElmer) according to the manufacturer’s protocol.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 0.05□μl of 5□mM stock compounds in DMSO were dispensed into assay microplates with a Janus 384 MDT NanoHead (PerkinElmer). Final concentration of the screening compounds in the assay was 16LμM ...
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bioRxiv - Plant Biology 2024Quote: ROS production was monitored by the use of a luminol-dependent assay as previously described (Butenko et al., 2014) using a Wallac 1420 VICTOR2 microplate luminometer (PerkinElmer). Arabidopsis leaves expressing 35S:HAE:YFP were cut into leaf discs and incubated in water overnight before measurements ...
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bioRxiv - Bioengineering 2023Quote: ... RNA integrity was analyzed using automated capillary electrophoresis (RNA Pico Sensitivity Assay, LabChip GX II Touch HT instrument, Perkin Elmer). Samples were stored at –80°C until further analysis.
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bioRxiv - Cell Biology 2020Quote: ... Lysis and luciferase activity detection was performed using the Gaussia-Juice Luciferase Assay (PJK Biotech, Kleinblittersdorf, Germany) according to the manufacturer’s description in white opaque 96-well plates (OptiPlate, PerkinElmer, Waltham, MA) and using a Synergy Mx microplate reader (BioTek ...
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bioRxiv - Bioengineering 2022Quote: Saturation assays for binding of K1K1H6 to recombinant MET-Fc protein were performed in 384-well microtiter plates (OptiPlate™-384, PerkinElmer© ...
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bioRxiv - Neuroscience 2021Quote: ... 100 µL of 2% ACN/0.1% TFA was added to the reaction mixture and the mixture was analyzed by mobility shift assay (LabChip EZ Reader II; PerkinElmer, U.S.A.). The kinase activity is the percentage of phosphorylated peptide signal over the total substrate peptide signal ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... All biomarkers were measured in triplicate (intra-assay coefficient of variability [CV] < 15% in all cases) using an EnVision microplate reader (PerkinElmer, Finland) and calibrated to the protein concentration in the sample ...
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bioRxiv - Biophysics 2021Quote: ... Validation assays for 5-HT5AR-Gi1 expression were performed as described above using 10nM [3H]-LSD (82.4 Ci/mmol, PerkinElmer, Waltham, MA).
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bioRxiv - Cell Biology 2022Quote: ... An equal volume of cell culture media and esterase substrate (100 μM in esterase assay media at pH 5) was transferred to a black walled clear bottomed plate (Perkin Elmer) and fluorescence was measured after 1 h.
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bioRxiv - Biochemistry 2019Quote: ... The BMAL TAD probe was diluted to a working concentration of 50 nM in assay buffer and binding was monitored by changes in fluorescence polarization with an EnVision 2103 multilabel plate reader (Perkin Elmer) with excitation at 531 nm and emission at 595 nm ...
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bioRxiv - Biophysics 2019Quote: AlphaScreen-based peptide recruitments assays were performed using streptavidin coated donor beads and anti-His-tag antibody coated AlphaLisa acceptor beads (Perkin Elmer). The assays were very sensitive to the distance of the N-terminus to the LXXLL NR-box motif and the use of a flexible Ahx linker ...