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Citations for PerkinElmer :
301 - 350 of 548 citations for Dengue Virus Serotype 2 Envelope Protein HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... 50 μL/well) was captured overnight at 4°C onto high-protein-binding ELISA plates (Spectraplate 96HB, Perkin Elmer). Plates were washed in PBS/Tween (0.05% v/v ...
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bioRxiv - Cancer Biology 2020Quote: ... shRNA expression with surrogate red fluorescent protein (RFP) was tracked by fluorescence (IVIS Spectrum In Vivo Imaging Station, PerkinElmer). For chloroquine xenograft experiments ...
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bioRxiv - Cell Biology 2021Quote: ... Media were replaced with fresh media supplemented with Easytag Express S35 Protein Labeling Mix (PerkinElmer, 100-200 µCi/mL) for 1 h ...
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bioRxiv - Neuroscience 2022Quote: ... The images of the targeting proteins were analyzed by a high content screening system (Operetta Enspire, Perkin Elmer, USA). The integral optical density (IOD ...
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bioRxiv - Microbiology 2022Quote: ... The membranes were incubated with primary and secondary HRP-conjugated antibodies in PBST (5% milk and 0.1% Tween-20) to detect the protein of interest by Western Lightning Plus ECL (PerkinElmer) and ImageQuant LAS 4000 imaging system (GE) ...
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bioRxiv - Cell Biology 2021Quote: ... cells were metabolically labelled for 20 min with 55 μ Ci/ml 35S- methionine/cysteine protein labelling mix (PerkinElmer). Cells were washed with PBS and cultured in DMEM (Invitrogen ...
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bioRxiv - Neuroscience 2021Quote: The level of MMP-9 in plasma was measured with an AlphaLISA kit for the human protein (#AL3138, PerkinElmer). AlphaLISA signals were measured using an Ensight Multimode Plate Reader (PerkinElmer).
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bioRxiv - Molecular Biology 2019Quote: ... The cells were then supplemented with 7.4 MBq of [35S] methionine and [35S] cysteine (EXPRE35S35S Protein Labeling Mix, [35S]-, PerkinElmer) and incubated for 1 h to specifically label newly synthesized mitochondrial proteins ...
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bioRxiv - Immunology 2020Quote: ... The cpms of immunoprecipitated protein was quantified using a 96-well Microbeta Trilux liquid scintillation plate reader (Perkin Elmer).
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... We next added 0.8 μl of FLAG-GST-transcription factor proteins to 384-well AlphaPlates using a NanoHead (PerkinElmer) and a Janus Workstation (PerkinElmer) ...
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bioRxiv - Microbiology 2019Quote: ... and the protein band reacting with the antiserum was detected using a chemiluminescence detection system (PerkinElmer, Waltham, MA, USA).
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bioRxiv - Microbiology 2021Quote: ... was used (at 1:20.000 dilution) for protein visualization in the presence of Western Lightning Plus-ECL enhanced chemiluminescence substrate (PerkinElmer). The WesternSure® Pre-stained Chemiluminescent Protein Ladder (Li-cor ...
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bioRxiv - Microbiology 2022Quote: ... cells were metabolically labeled for 16 h with 100 μCi/mL [35S] methionine-cysteine ([35S] protein labeling mix; PerkinElmer) in Dulbecco’s modified Eagle’s medium lacking methionine and cysteine and supplemented with 10% of dialyzed fetal bovine serum and 1X GlutaMAX™ (ThermoFisher Scientific) ...
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bioRxiv - Biochemistry 2022Quote: ... Cells then were radiolabeled for 15 minutes with 143 µCi/dish EasytagTM 35S Express Protein Labeling Mix (Perkin Elmer) and chased for indicated times in DMEM supplemented with 5 mM unlabeled methionine and cysteine ...
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bioRxiv - Physiology 2022Quote: ... 60-100 µg protein was incubated with 100 μl TG substrate (25 nmol triolein/assay and 40,000 cpm/nmol 14C-triolein; PerkinElmer) and 35.5 μg mixed micelles of phosphatidylcholine and phosphatidylinositol (3:1 ...
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bioRxiv - Neuroscience 2022Quote: GPCR activation assays were performed using CHO-K1 cells stably expressing the luminescent Ca2+ indicator aequorin and the promiscuous Gα16 protein (ES-000-A24 cell line, PerkinElmer). The cells were transfected with a dmsr-7/pcDNA3.1 plasmid using lipofectamine LTX and Plus reagent (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... aliquots of derivatized lysates containing 15 µg of protein were spotted on a Whatman Protran nitrocellulose membrane (Perkin Elmer). Blots were blocked as above ...
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bioRxiv - Biochemistry 2023Quote: ... 10 μM purified IKK2 protein was first autophosphorylated in the presence of 100-200 nM of γ32P-ATP (PerkinElmer) in kinase assay buffer for 1 hour at 30°C ...
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bioRxiv - Biophysics 2021Quote: ... emission 535 nm) measured for 1 hour at 2 minute intervals on an EnVision Xcite 2104 (PerkinElmer). Enzymatic activity of each mutant was measured with (+ Palm-CoA ...
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bioRxiv - Immunology 2019Quote: ... 2 × 103 cells were plated in pre-coated 96 well plates (CellCarrier-96 Ultra Microplates, black, PerkinElmer) following the same procedure.
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bioRxiv - Systems Biology 2021Quote: ... columns 2-23 were used) of tissue-culture treated black CellCarrier-384 Ultra Microplates (Perkin Elmer 6057302) using a 12-channel electronic multichannel 200 μL pipettor [Sartorius] ...
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bioRxiv - Cell Biology 2020Quote: ... 1 mM MnCl2) supplemented with 10 μM ATP and 2 μCi γ-32P-ATP (blu002z250uc, Perkin Elmer) in 15μL reactions ...
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bioRxiv - Cell Biology 2020Quote: ... and the wells were covered immediately using filter papers soaked with hyamine hydroxide (Perkin Elmer, 2-19361). After incubation in a fume hood for at least 12 hours to reach saturation ...
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bioRxiv - Cell Biology 2020Quote: ... and the wells were covered immediately using filter papers soaked with hyamine hydroxide (Perkin Elmer, 2-19361). After incubation in a fume hood for at least 12 hours to reach saturation ...
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bioRxiv - Immunology 2022Quote: ... Uptake was initiated by addition of 1 μCi [3H]2-DG (25-30 Ci/mmol, PerkinElmer, NET549) and 0.1 mM unlabeled 2-DG (Sigma ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were loaded with 2 μCi/ml D-[2,3-3H]aspartate (Perkin Elmer, Waltham, MA, cat. #NET50100) in MEM +HIHS cell culture medium ...
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bioRxiv - Developmental Biology 2023Quote: ... Anesthetized (2% isoflurane vaporized in 1 L/min O2) dams were imaged (IVIS Lumina III, Perkin Elmer) with their ventral side facing the charge-coupled device camera (15 cm field of view ...
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bioRxiv - Plant Biology 2023Quote: ... resuspended in 2 N NaOH and mixed with 5 volumes scintillation fluid (Ultima Gold™ XR, PerkinElmer) for 14C-CPM counting in a TRI-CARB liquid scintillation counter (PerkinElmer ...
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bioRxiv - Neuroscience 2023Quote: ... 2 µL of cell culture medium or standard solution was added to an Optiplate-384 microplate (PerkinElmer). 21µL of 10X mixture including acceptor beads and biotinylated antibody was then added to the wells with culture media or standard solution ...
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bioRxiv - Cell Biology 2023Quote: ... cells on the SPA plate were suspended in 1 mL of DDW with 2% glusulase (PerkinElmer, #NEE154001EA) and digested with shaking at room temperature for 3 hr ...
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bioRxiv - Cancer Biology 2021Quote: ... 30 ug of protein lysate was resolved by SDS-PAGE and transferred onto a Protran pure 0.2 micron nitrocellulose (Perkin Elmer). The membranes were incubated for 1h in 5% skimmed milk and probed with specific primary antibodies (Table S1 ...
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bioRxiv - Cell Biology 2020Quote: ... and labeled for 5 min with with 1 μL per OD of cells of EXPRESS 35S Protein Labeling Mix (PerkinElmer) for 5 or 10 min ...
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bioRxiv - Immunology 2021Quote: ... the unbound proteins were washed and luciferase activity was measured using Nano-Glo substrate (Nanolight) in an Envision 2103 microplate luminometer (PerkinElmer).
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bioRxiv - Genetics 2021Quote: ... Grain protein content (%) and grain yield (t/ha) was collected using a Perten DA 700 NIR analyzer (Perkin Elmer, Sweden) and Wintersteiger Nursery Master combine (Ried im Innkreis ...
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bioRxiv - Molecular Biology 2020Quote: ... The protein pellet was then re-suspended in PBS and incubated in 4 mL of scintillation fluid (Insta-Gel Plus, PerkinElmer) for 24 h (to homogenize the samples ...
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bioRxiv - Molecular Biology 2019Quote: Cells were incubated with complete growth media supplementing with EasyTagTM Express Protein Labeling Mix containing both [35S]-L-methionine and [35S]-L-cysteine (PerkinElmer) at 10 μCi/ml for 30 min at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... 1.37 μg/mL of RBD and 5 μg/mL of Ecto) and incubated overnight in protein-binding 96-well plates (PerkinElmer). The next day ...
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bioRxiv - Biochemistry 2020Quote: ... newly synthesized proteins in the cells were labeled by treating with 150 μCi/ml of 35S-methionine/cysteine (EXPRE35S35S Protein Labeling Mix, NEG772, PerkinElmer) apically for 45 min (pulse labeling) ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were pulse-labeled for the indicated times with 143 µCi/dish EasytagTM 35S Express Protein Labeling Mix (Perkin Elmer). After the pulse ...
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bioRxiv - Neuroscience 2020Quote: ... 3 and 6 hr later cells were labeled for 5 min with 1 mCi/ml 35S Protein Labeling Mix (PerkinElmer). Lysates from 25-ml culture triplicate samples were analyzed by SDS-PAGE and autoradiography.
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bioRxiv - Neuroscience 2022Quote: ... The samples were incubated for 30 min at 37 °C in the presence of the EasyTag Express Protein Labeling Mix 35S (PerkinElmer), pelleted ...
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bioRxiv - Synthetic Biology 2019Quote: ... Radioactive counts from TCA-precipitated samples was measured by liquid scintillation to then quantify soluble and total yields of each protein produced as previously reported (MicroBeta2; PerkinElmer).39,40
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bioRxiv - Microbiology 2020Quote: ... 200 μCi (7.4 MBq) [35S]-methionine/L-[35S] cysteine using EasyTag™ EXPRESS35S Protein Labeling Mix (PerkinElmer, Groningen, the Netherlands) was added to the medium ...
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bioRxiv - Synthetic Biology 2020Quote: ... Radioactive counts from TCA-precipitated samples was measured by liquid scintillation to then quantify soluble and total yields of each protein produced as previously reported (MicroBeta2; PerkinElmer).27,30
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bioRxiv - Immunology 2021Quote: ... The excitation and emission wavelengths for each protein were determined prior to the stopped-flow experiments using an LS55 luminescence spectrophotometer (PerkinElmer). The oxidant concentration range required to lead to fluorescence changes for each protein was also determined.
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bioRxiv - Cell Biology 2019Quote: ... loads were adjusted and proteins were resolved by SDS-PAGE and analyzed by western-blot (Western Lightning Plus-ECL kit; PerkinElmer). For Flag immunoprecipitations ...
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bioRxiv - Immunology 2021Quote: ... The proteins were then separated with SDS-PAGE and detected by phosphor imaging (Cyclone Plus storage phosphor system, Perkin Elmer).
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bioRxiv - Cancer Biology 2022Quote: ... 15 or 10-20% SDS-PAGE gels and proteins were transferred onto PVDF membranes (0.45 μm, Perkin Elmer, Waltham, MA) and blocked with 5% BSA/TBS-T for 2 hours at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... cells were cultured in 6-well plates and metabolically labeled (Easytag Express 35S-Met-Cys protein labeling mix (Perkin Elmer)) with 0.2 mCi/ml for various times ...
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bioRxiv - Bioengineering 2023Quote: ... of approximately 5.7 ± 3.0 pmol/mg protein measured by the manufacturer using [3H]-DAMGO (ES-542-C OP3 ValiScreen, PerkinElmer, Waltham, MA) were obtained and cultured for a series of binding assays ...