Labshake search
Citations for PerkinElmer :
551 - 591 of 591 citations for Cow Porphobilinogen deaminase HMBS ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... These purified ribosome footprints were used as input for the NEXTflex small RNA-seq kit v3 (Perkin Elmer, cat# NOVA-5132-06). The resulting cDNA was amplified by 11-16 cycles of PCR with a barcode incorporated in the primer ...
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bioRxiv - Immunology 2024Quote: ... 1:1 mixed with maleate buffer (R2a from the kit)) was added and incubated at room temperature and measured kinetically on an Envision microplate reader (PerkinElmer, Waltham, MA) at 340 nm for 20 min ...
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bioRxiv - Cancer Biology 2024Quote: ... Peripheral blood samples or buffy coat extracts were extracted in Auria Biobank for all patients for DNA extraction and genomic sequencing to serve as germline reference for identifying somatic genomic aberrations using Chemagic DNA Blood Kit Special (PerkinElmer Inc., USA) and Chemagic 360 instrument (PerkinElmer Inc. ...
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bioRxiv - Cell Biology 2020Quote: LH levels in the medium were measured by means of a time-resolved fluorometric immunoassay with rat LH assay kits (National Hormone and Peptide Program, NHPP) and the Delfia system (Perkin Elmer, Waltham, MA) as reported previously (Kawaminami et al. ...
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bioRxiv - Evolutionary Biology 2021Quote: ... biotin-labelled probes were visualized by streptavidin-horseradish peroxidase (HRP) conjugates with Cyanine 5 Plus Amplification Reagent in a TSA kit as the substrate (PerkinElmer, Boston, MA, USA). Sense probes labelled with DIG and biotin by SP6 RNA transcription kit were used as control ...
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bioRxiv - Cell Biology 2021Quote: ... the RBP-RNA complex was purified and the bound RNA eluted for subsequent Illumina library preparation (NEXTFLEX small RNA library preparation kit v3, PerkinElmer #NOVA-5132-06) and paired-end (40×43 cycles ...
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bioRxiv - Neuroscience 2020Quote: ... Cux2 (Atlas Antibodies, Stockholm, Sweden; rabbit, 1:200; detected with ABC kit [Vector Laboratories, Burlingame, CA, USA] and TSA [PerkinElmer, Waltham, MA, USA]), Dcx (Santa Cruz Biotechnology ...
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bioRxiv - Biochemistry 2020Quote: The amounts of cAMP in the cytosol were measured by using the time-resolved fluorescence resonance energy transfer (TR-FRET) kit from Perkin Elmer (EnVision Xcite), according to the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2020Quote: ... Reverse transcription was performed using random hexamer primers as described by the manufacturer (GeneAmp RNA PCR Kit from Perkin Elmer Cetus, Norwalk, CT). PCR-mastermix containing the specific primers and Taq DNA polymerase (HotStar Taq ...
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bioRxiv - Immunology 2020Quote: ... The length distribution of the cDNA libraries was monitored using DNA High Sensitivity Reagent Kit on the Perkin Elmer Labchip GX system (PerkinElmer, Waltham, MA, USA). The libraries were qPCR quantified (Kapa Biosystems ...
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bioRxiv - Neuroscience 2021Quote: IP1 accumulation assay: Inositol monophosphate accumulation was determined using the IP-One HTRF (Homogenous Time Resolved Fluorescence) kit (Cisbio Bioassays, Perkin Elmer, Codolet, France) according to the manufacturer’s recommendations (112) ...
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bioRxiv - Developmental Biology 2022Quote: Intracellular ATP and extracellular lactate levels were measured for each NCC primary cultures after Seahorse analysis using an ATPlite Bioluminescence assay kit from PerkinElmer (cat. no. 6016943) and the Lactate Fluorometric Assay kit from BioVision (cat ...
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bioRxiv - Physiology 2020Quote: ... 1 ng of eluted and purified DNA was used to prepare DNA sequencing library with the Nextflex rapid DNA seq kit 2.0 (Perkin Elmer, NOVA-5188-01) on the NextSeq 500 system (Illumina ...
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bioRxiv - Developmental Biology 2020Quote: ... we followed the protocol provided by the commercial company except that all samples were stained in 1 to 80 dilution of Cy3-conjugated or FLU-conjugated Tyrimide substrates for 60 minutes (Perkin Elmer NEL753001 Kit).
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bioRxiv - Genetics 2020Quote: ... and used as input for small RNA library prep using the NEXTflex Small RNA-Seq Kit v3 (Bioo Scientific - PerkinElmer NOVA-5132-06). Libraries were prepared according to manufacturer’s instructions (V19.01) ...
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bioRxiv - Plant Biology 2020Quote: The AlphaScreen® (Amplified Luminescent Proximity Homogeneous Assay) was carried out using an AlphaScreen® FLAG® (M2) Detection Kit (Perkin Elmer, MA) to detect protein-protein interactions ...
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bioRxiv - Developmental Biology 2020Quote: ... RNA hybridization were performed on dechorionated 20 hpf embryos with the tyramide amplification kit (TSA Plus Cyanine 3 System, Perkin Elmer, Boston, MA). Briefly ...
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bioRxiv - Immunology 2020Quote: ... often from an ImmPRESS® polymer detection kit followed by colour development using a tyramide signal amplification system kit with cyanine (Cy)3 or fluorescein (1:50 dilution; PerkinElmer, Waltham, MA, USA). For detection of the second antigen in dual immunofluorescence ...
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bioRxiv - Microbiology 2021Quote: DNA library preparation was carried out according to the manufacturer’ ss protocol using the NEXTflex™ ChIP-Seq Kit (NOVA-5143-01) (PerkinElmer, Hamburg, Germany) and the NEXTflex™ ChIP-Seq Barcodes (NOVA-514122 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Extracted DNA was sent to Texas A&M Agrilife Bioinformatics and Genomics Service (College Station, TX, USA) for whole-genome library preparation using a NEXTFLEX Rapid XP DNA-Seq Kit HT (PerkinElmer, Waltham, MA, USA). Libraries were sequenced at the Texas A&M Agrilife Bioinformatics and Genomics Service on one NovaSeq 6000 S4 lane at 26.1 ± 5.8 depth of coverage per sample across the full genome (2 s.d. ...
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bioRxiv - Microbiology 2022Quote: Quality and quantity of the extracted RNA samples were analyzed with LabChip GX Touch HT RNA Assay Reagent Kit (PerkinElmer, Waltham, MA, USA) and Qubit RNA BR kit (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... cDNA libraries were prepared from RNA extracts with a minimum RNA integrity number (RIN) of 9.5 using the NEXTFLEX® Combo-Seq mRNA/miRNA Kit (PerkinElmer®, Waltham, MA). Libraries were quantified using a KAPA Library Quantification Kit (Roche Sequencing and Life Science ...
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bioRxiv - Genomics 2022Quote: ... 1 ng of eluted and purified DNA was used to prepare libraries with the Nextflex rapid DNA seq kit 2.0 (Perkin Elmer, #NOVA-5188-01) on the Illumina NextSeq500 system using a single read 100 bp protocol ...
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bioRxiv - Biochemistry 2023Quote: The expression of IL-6 and TNF-α in culture supernatants was detected using the corresponding AlphaLISA Immunoassay Kit (PerkinElmer, AL223C and AL208C) according to the manufacturer’s protocol.
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bioRxiv - Genomics 2024Quote: ... size-selected sample was reamplified with 25 μL of NEXTFLEX PCR Master Mix 2.0 and 2 μL of 1:10 diluted NEXTFLEX Primer Mix 2.0 as described in the NEXTFLEX Cell free DNA-Seq Kit (V2) (Perkin Elmer, Pittsburgh, Pennsylvania, USA), step C PCR amplification ...
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bioRxiv - Evolutionary Biology 2019Quote: ... followed by the amplification of the signal with fluorophore-conjugated tyramides (1:100 TSA reagent diluents, Perkin Elmer TSA Plus Cy3 or Cy5 Kit). Residual enzyme activity was inhibited via 45-minute incubation in 0.1% hydrogen peroxide in PTW followed by four PTW washes prior to addition and development of the second peroxidase-conjugated antibody76.
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bioRxiv - Biochemistry 2020Quote: ... Library preparation for deep sequencing was performed using the NextFlex Rapid Directional qRNA-Seq Library Prep Kit (Perkin Elmer, Bioo Scientific, NOVA-5130-01D) according to the manufacturer’s instructions using 7 unique barcodes ...
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bioRxiv - Microbiology 2019Quote: ... The length distribution of the cDNA libraries was monitored using a DNA High Sensitivity Reagent Kit on the Perkin Elmer Labchip (Perkin Elmer, Waltham, MA, USA). All samples were subjected to an indexed paired-end sequencing run of 2×51 cycles on an Illumina HiSeq 2000 system (Illumina ...
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bioRxiv - Genetics 2020Quote: ... Genomic DNA was fragmented with a COVARIS S2 and an Illumina-compatible library was prepared with the NEXTflex Bisulfite Library Prep Kit for Illumina Sequencing (Bioo Scientific/PerkinElmer, Austin, Texas, U.S.A). Bisulfite conversion was performed using the EZ DNA Methylation Gold Kit (Zymo Research ...
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bioRxiv - Cancer Biology 2021Quote: ... For multiplexed immunohistochemistry signals were detected using Opal™ 4-Plex Kit according to the manufacturer’s protocol (catalog no. NEL810001KT; Perkin Elmer, Waltham, MA, USA). For more details see Supplemental Materials section.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Ligand-stimulated cAMP accumulation in the presence or absence of 0.5 mM 3-isobutyl-1-methylxanthine (IBMX) was measured after the indicated times of stimulation using LANCE® cAMP Detection Kit (Perkin Elmer Life Sciences) and a Mithras LB 940 multimode microplate reader ...
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bioRxiv - Cell Biology 2022Quote: ... determined by competitive immunoanalysis using Time-Resolved Fluorescence Resonance Energy Transfer (TR-FRET) as a detection system (Lance Ultra cAMP kit from Perkin Elmer, Waltham, MA, USA) according to the manufacture instructions ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: The level of phosphorylated extracellular signal-regulated protein kinase 1/2 (pERK1/2) was detected using the AlphaScreenTM SureFire Kit (PerkinElmer Life and Analytical Sciences). Briefly ...
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bioRxiv - Microbiology 2023Quote: ... Compounds were incubated for 48 h and cell viability was determined by measuring the adenosine triphosphate (ATP) content of the cells using the ATPlite kit (PerkinElmer, Waltham, MA, Cat: 6016941) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Sequencing libraries were prepared from the 10.5 μl reactions using the NEXTflex Small RNA-Seq Kit v3 (Perkin Elmer/Bioo Scientific, #NOVA-512-05) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... Illumina libraries were prepared using the ResolveOME library preparation kit (BioSkryb Genomics Inc.) with NEXTFLEX Unique Dual Index Barcodes (PerkinElmer Applied Genomics, NOVA-534100). Libraries were sequenced at low-pass (2x 50bp paired end ...
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bioRxiv - Genetics 2024Quote: ... RNA quality was assessed in an Agilent 2100 Bioanalyzer and strand-specific cDNA libraries were made with NEXTflex™ Rapid Directional qRNA-Seq™ Kit (PerkinElmer, formerly BiooScientific) with molecular indexing ...
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bioRxiv - Developmental Biology 2024Quote: ... Vectastain Elite ABC kit) followed by the tyramide signal amplification reaction (Cy3 fluorescent dye in amplification diluent; 1:50) (Perkin Elmer Life Sciences, NEL753001kt). To mark the SG and the apical membrane ...
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bioRxiv - Microbiology 2021Quote: ... The level of HIV-1 replication was determined by measuring p24 antigen in the culture supernatant using a kit (Perkin Elmer Inc, Shelton, CT, USA).
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bioRxiv - Genomics 2022Quote: SARS-CoV-2 genome libraries were constructed by using the NEXTFLEX® Variant-Seq™ SARS-CoV-2 kit with the automated high-throughput liquid handler Sciclone® G3 NGS (Perkin Elmer, USA) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2020Quote: ... The DNA concentration was measured using the Picogreen method (Invitrogen Quant-iT™ Picogreen dsDNA Assay Kit Reference No. P11496 on Perkin Elmer 2030 Multilabel Reader Victor X3) and DNA integrity number (DIN ...