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Citations for PerkinElmer :
551 - 600 of 811 citations for Collagen Alpha 1 XVIII Chain Endostatin Mouse HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: DK1042 was cultured with isolate P9_31 in 1× LBGM or 0.1× LBGM in 24-well imaging microtiter plates (PerkinElmer). Wells were imaged on a Leica SP8 confocal microscope (Leica Microsystems ...
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bioRxiv - Biochemistry 2023Quote: ... The plates were then incubated at room temperature for 1 h and read on an Envision 2104 (PerkinElmer) using the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Cell mixtures were incubated for 1 hour at 37°C with 0.5% v/v glusulase (Perkin Elmer, NEE154001EA) to digest cell walls of vegetative cells and asci ...
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bioRxiv - Microbiology 2023Quote: The ROS production by macrophages was measured by chemiluminescence in the presence of 5-amino-2,3-dihydro-1,4-phthalazinedione (luminol) using a thermostatically (37°C) controlled for 1 hr (Envision, PerkinElmer). For nitrite release ...
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bioRxiv - Pathology 2024Quote: ... 2% fetal bovine serum and 1% penicillin-streptomycin mix) in a microscopy microtiter plate (CellCarrier-96 ultra, PerkinElmer). On the following day ...
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bioRxiv - Biophysics 2021Quote: ... A 50 μl reaction mixture contains 1.6 mM [14C]-G3P (specific activity at 129.3 mCi mmol-1, PerkinElmer, USA), 0.8 mM CDP-DAG (Avanti ...
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bioRxiv - Cell Biology 2020Quote: ... image stacks (5 × 1 μm optical sections) were acquired on a spinning disc confocal system (Ultraview Vox, Perkin Elmer) with a 60× 1.4 NA oil-immersion objective (Nikon ...
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bioRxiv - Developmental Biology 2021Quote: ... Perkin-Elmer) coupled with fluorogenic substrate Alexa Fluor 594 tyramide for 30 s (1:200, TSA kit; Perkin Elmer). F-actin was stained with rhodamine-conjugated phalloidin (1:200 ...
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bioRxiv - Neuroscience 2021Quote: ... Oocytes were lysed with 1M NaOH and 1%SDS before the addition of 3 mL scintillant (Optiphase HisSafe, PerkinElmer), and l-[3H]glutamate transport was measured using a MicroBeta TriLux scintillation counter (PerkinElmer).
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bioRxiv - Microbiology 2020Quote: ... programmed with 1 µg DNA/50 µL and labeled with 20 µCi of L-[35S]-methionine (EasyTag, Perkin Elmer). Reactions were incubated at 30 °C for 30 min before adding L-methionine (1 mM final ...
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bioRxiv - Cell Biology 2019Quote: ... p-ERK detection was performed using the AlphaLISA SureFire Ultra p-ERK 1/2 (Thr202/Tyr204) Assay Kit (PerkinElmer) per the manufacturer’s specifications in 1/2-area white 96-well plates ...
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bioRxiv - Developmental Biology 2019Quote: ... slides were washed in TNT and treated with Fluorescein Amplification Reagent (Perkin Elmer, 1:50 in Plus Amplification Diluent) for 15 min at room temperature ...
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bioRxiv - Microbiology 2020Quote: 2.5 × 105 THP-1 cells were seeded on 24-well optical bottom tissue culture plates (Perkin Elmer, 1450-606), treated with 100nM phorbol 12-myrystate-13-acetate (PMA ...
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bioRxiv - Immunology 2021Quote: ... of the abdominal region was measured (exposure=1 sec., F/stop= 1.2, FOV=E, binning=medium, emissions filter=open) using Living Image software (PerkinElmer). Survival of the mice was recorded until 20 days after infection.
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bioRxiv - Cancer Biology 2021Quote: ... Slides were washed 3 times in PBS and incubated with tyramide Cy3 1:150 in amplification buffer (Perkin Elmer) for 10 min ...
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bioRxiv - Cancer Biology 2022Quote: ... ATP was measured in supernatants according to the protocol described by the ATPlite 1 step Luminescence Assay System (PerkinElmer). Luminescence readings were obtained from a Bioteck Synergy HT plate reader ...
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bioRxiv - Neuroscience 2021Quote: ... incubated with 3% of H2O2 for 30 minutes at RT and blocked 1 hour RT with NEN blocking buffer prepared according to the manufacturer’s protocol (FP1012 PerkinElmer). AntiDIG antibody conjugated to HRP (1:2000 ...
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bioRxiv - Neuroscience 2021Quote: ... incubated with 3% of H2O2 for 30 minutes at RT and blocked 1 hour RT with NEN blocking buffer prepared according to the manufacturer’s protocol (FP1012 PerkinElmer). AntiDIG antibody conjugated to HRP (1:1500 ...
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bioRxiv - Cell Biology 2020Quote: Fully differentiated C2C12 MS2-CAS myotubes were starved in serum-and glucose-free DMEM for 2 hrs before a 3-hr incubation in low-glucose DMEM/fatty acid-free BSA 0.2% supplemented with radiolabeled glucose (Glucose, D-[3-3H]; 1 μCi/ml; PerkinElmer). Then ...
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bioRxiv - Cancer Biology 2022Quote: ... were were dispensed per well 1 h prior to imaging at Cytation5 image cytometer or Opera Phenix (Perkin Elmer) confocal screening microscope.
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bioRxiv - Molecular Biology 2021Quote: ... The medium was removed and 125I-GLP-1(7-36)NH2 (60 pM) or 125I-GCG (40 pM) (PerkinElmer) and increasing concentrations unlabeled tirzepatide or peptide 20 were added for overnight incubation at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... slices were incubated in a goat anti-rabbit horse radish peroxidase-conjugated IgG (1:200, NEF812001EA, PerkinElmer, Waltham, MA) for 30 min ...
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bioRxiv - Plant Biology 2020Quote: ... Two 100-μl fractions were collected in 50 μl of 1.5 M Tris-HCl (pH 8.0) and 1 ml of Ultima Gold LLT scintillation cocktail (Perkin Elmer). Radioactivity was measured using a liquid scintillation analyser (Packard).
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bioRxiv - Biochemistry 2021Quote: ... 20 μL of protein mixture was added to each well of a 1/2 area 96-well OptiPlate (PerkinElmer). Compounds were then added from DMSO stock to protein mixture (0.4 μl/well ...
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bioRxiv - Biochemistry 2021Quote: ... 20 μL of protein mixture was added to each well of a 1/2 area 96-well OptiPlate (PerkinElmer). FKBP12F36V competitor or p300-BRD competitor was then added from DMSO stock to protein mixture (0.4 μl/well ...
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bioRxiv - Neuroscience 2021Quote: ... Slides were washed again and incubated with the appropriate Opal tertiary antibody (1:100 in amplification diluent, Perkin Elmer) for 60 min ...
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bioRxiv - Biochemistry 2021Quote: ... Lysates were incubated for 1 hour at room temperature before FRET was detected using an Envision plate reader (PerkinElmer). Emission of Lumi4TM-Tb cryptate was detected at 620 nm and emission of d2-conjugated IP1 at 665 nm ...
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bioRxiv - Developmental Biology 2020Quote: ... incubated 1h in blocking buffer and then incubated overnight with an anti-DNP-POD antibody (1:1000, Perkin Elmer). The next day embryos were washed in PBS-Tween ...
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bioRxiv - Bioengineering 2021Quote: ... background luminescence was measured for each rat (1 sec exposure, field of view D, PerkinElmer IVIS, Waltham, MA, USA). Both knees were then injected with either 50 μL NanoLuc™ Luciferase (NL ...
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bioRxiv - Microbiology 2021Quote: ... was used (at 1:20.000 dilution) for protein visualization in the presence of Western Lightning Plus-ECL enhanced chemiluminescence substrate (PerkinElmer). The WesternSure® Pre-stained Chemiluminescent Protein Ladder (Li-cor ...
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bioRxiv - Physiology 2022Quote: ... EP3R immunoreactivity was visualized by reaction with FITC-conjugated tyramide (1:50; SAT701001KT; Tyramide Signal Amplification FITC Systems, PerkinElmer) for 3 min ...
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bioRxiv - Neuroscience 2022Quote: ... was diluted at 1 µg/mL in PBS and passively absorbed on multiwell plates (SpectraPlate-384 HB, Perkin Elmer) overnight at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... assays were carried out by incubating 500ng of methyltransferases Rv2067c or DOT1L with 1–2 μg of recombinant mammalian histones or MtHU and 80μCi 3H-SAM (PerkinElmer) in a buffer containing 50 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Microbiology 2023Quote: ... normalized by cell OD600 (final OD=0.4) and diluted 1: 100 into 150 μl of fresh TSB media in flat-bottomed 96-well plates (PerkinElmer). The cultures were overlaid with 100 μl of mineral oil (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... for 1 h at 37 °C and imaged with the Opera Phenix High Content Screening System (#HH14000000, Perkin Elmer). Number of counted nuclei was determined with the image analysis software Harmony (PerkinElmer ...
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bioRxiv - Biochemistry 2023Quote: ... 100 nM of WT FCE or mutant K286M was incubated with 1 μM of 32P-α-GTP (Perkin Elmer) in a 20 μL reaction containing 1x RNA capping buffer at room temperature for 1 minute ...
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bioRxiv - Biochemistry 2023Quote: ... The samples were then separated on a HayeSep column (7’ HayeSep N 1/8’’ Sf; PerkinElmer Inc., Waltham, USA), followed by molecular sieve (9’ Molecular Sieve 13x 1/8’’ Sf ...
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bioRxiv - Cancer Biology 2023Quote: ... 4T-1-Luc3 tumors and metastases were monitored by bioluminescence imaging on an IVIS Lumina III System (Perkin Elmer) twice a week after tumor implantation ...
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bioRxiv - Neuroscience 2023Quote: ... pH 7.5/1 μM fluoxetine) containing 50 pM of the DAT ligand (125I) RTI55 (PerkinElmer Life Sciences, Boston, USA) for 60 min ...
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bioRxiv - Microbiology 2023Quote: ... was added to the media at a 1:10,000 dilution and images were acquired using the Opera Phenix High-Content Screening System (PerkinElmer). Analysis was performed using Harmony 191 High-Content Imaging and Analysis Software (PerkinElmer ...
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bioRxiv - Bioengineering 2023Quote: We used hairless SKH-1 mice for live animal imaging on IVIS Spectrum In vivo Imaging System (Perkin Elmer). For ovalbumin quantification ...
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bioRxiv - Immunology 2023Quote: ... Samples were incubated in triplicate (106 per sample) with 1 µCi of 2-[1,2-3H]-deoxyglucose (20 Ci/mmol; PerkinElmer) in uptake buffer for 4 min at room temperature and immediately spun through a layer of bromo-dodecane into a layer of 20% perchloric acid/8% sucrose to stop the reaction and separate cells from unincorporated 2-[3H]-deoxyglucose ...
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bioRxiv - Biochemistry 2024Quote: ... cells were washed 3 times with wash buffer (PBS + 1 mM CaCl2 + 0.1% BSA) before adding Microscint-20 (PerkinElmer) for tritium concentration measurement using a TriLux MicroBeta2 plate reader (PerkinElmer) ...
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bioRxiv - Plant Biology 2023Quote: ... samples were incubated for 1h in 1:50 of a digoxigenin-tyramide signal amplification solution (TSA Plus DIG, PerkinElmer) prepared according to manufacturer instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Slides were then washed three times with TNT buffer (TN buffer + 0.05% Tween-20) and incubated with FITC-tyramide diluted 1:100 in Amplification reagent (PerkinElmer) for 10 minutes at room temperature ...
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bioRxiv - Microbiology 2024Quote: The lipid composition of bacterial strains was determined after labeling with [1-14C] acetate (60 mCi/mmol; Perkin Elmer), [32P] phosphate (1 Ci/mmol ...
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bioRxiv - Cancer Biology 2021Quote: In vitro methyltransferase assays were performed using 30 ul reaction buffer (50 mM Tris-HCl pH 7.8, 50 mM KCl, 5 mM MgCl2) with 1 ul 3H-AdoMet (Perkin Elmer, specifific activity = 55-85 Ci/mMole,0.55 μCi/ul ...
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bioRxiv - Cell Biology 2020Quote: ... and labeled for 5 min with with 1 μL per OD of cells of EXPRESS 35S Protein Labeling Mix (PerkinElmer) for 5 or 10 min ...
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bioRxiv - Molecular Biology 2020Quote: ... the reactions were started by adding 5× NTP mixture (1 mM for ATP, CTP and GTP, 0.1 mM for UTP) containing 2 µCi of [α−32P] UTP (PerkinElmer). Transcription reactions were performed at 37°C for 20 min ...
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bioRxiv - Neuroscience 2021Quote: ... Molecular weight bands were visualized by incubation with peroxidase-conjugated anti-mouse or anti-rabbit secondary antibody (1:5000; Jackson Immuno Research Laboratories) followed by treatment with the ECL-plus reagent (Western Lighting; PerkinElmer) and exposure on G:Box Chemi XRQ system (Syngene) ...