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Citations for PerkinElmer :
51 - 100 of 1708 citations for Cell Division Cycle 2 Like Protein Kinase 6 CDC2L6 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... Eµ-Myc lymphoma cells grown in 6-well plates were spiked with 0.2 μCi/mL [1-14C]-acetate (PerkinElmer; NEC084H001MC) for the final 4 h of the experimental period ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were then labelled by adding the EXPRE35S35S Protein Labeling Mix (PerkinElmer EasyTagTM NEG772) at a ratio of 9 μL per mL of the concentrated culture ...
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bioRxiv - Genetics 2022Quote: ... To metabolically label the cells 5uL of EasyTag EXPRESS 35S protein labeling mix (PerkinElmer), was added to 10mL of SPO cultures and incubated with shaking for 10 minutes at 30°C ...
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bioRxiv - Cell Biology 2022Quote: LTE cell-free protein expression in combination with AlphaLISA® approach (Perkin Elmer, AUS) was performed for pairwise protein interaction analysis of PER2 and CRY2 proteins with CAVIN3 and CAVIN1 protein variants ...
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bioRxiv - Cancer Biology 2022Quote: ... at 2×104 cells/well into a CellCarrier-384 Ultra Microplate (Perkin Elmer). Cells were incubated at 37 °C in a humidified atmosphere and 10 % CO2 for 3 days.
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bioRxiv - Microbiology 2021Quote: ... The mixture was subjected to 40 cycles of amplification using a DNA thermal cycler 2720 (Perkin Elmer). For duplex PCR of the HPV L1 gene and human beta-actin gene ...
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bioRxiv - Neuroscience 2020Quote: ... 6-[3H]-DA (Perkin Elmer Life Sciences, USA) as previously described (Jensen et al. ...
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bioRxiv - Neuroscience 2023Quote: ... 6-[3H]-DA (Perkin Elmer Life Sciences, USA) was used to obtain saturation DA uptake curves ...
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bioRxiv - Microbiology 2020Quote: ... 293FT cells (2 × 104 cells) were plated in 96-well View Plates (Perkin Elmer Life Sciences, Waltham, MA, USA). Five hours later ...
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bioRxiv - Biochemistry 2021Quote: Polyacrylamide gel slices (1-2 mm) containing the purified proteins were prepared for mass spectrometric analysis using the Janus liquid handling system (PerkinElmer, UK). Briefly ...
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bioRxiv - Neuroscience 2021Quote: ... or 3.3 mM (Figure 5-figure supplement 1 and 8) ATP and 5 µM ProfilerPro Kinase Peptide Substrate 11 5-FAM-KKLNRTLSVA-COOH (PerkinElmer, U.S.A.), in the presence or absence of 0.66 µM CaM (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2021Quote: ... Table 9) and the compounds added to plates using the then kinase compounds added by the Sciclone ALH3000 robot (Perkin Elmer). Organoids were imaged using the Cytation5 (BioTek ...
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bioRxiv - Molecular Biology 2023Quote: ... incubation with a 32P-labelled oligodeoxyribonucleotide probe complementary to the target RNAs (32P-labelled using T4 polynucleotide kinase and [g 32P]-ATP [Perkin Elmer]) overnight in 37°C in SES1 buffer ...
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bioRxiv - Cancer Biology 2021Quote: ... sections were stained for NICD1/KRT14/DNA or NICD1/Ki67/DNA and epithelial thickness measured and cell counting performed using Volocity 6 software (Perkin Elmer). Cell density was measured by counting the number of nuclei identified across the width of the epithelium on high resolution images of cryosections stained with DAPI and keratinocyte markers such as KRT4 and ITGA6 and dividing this number by the measured surface area.
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bioRxiv - Cell Biology 2021Quote: ... Radioactive counts from the media that remain soluble after TCA precipitation (peptide fragments) and pellets from the cells (total non-degraded cell proteins) were measured in a TopCount radioactivity detector (Perkin Elmer). A “protein degradation index” was calculated as the ratio between the counts detected in the soluble media and the counts detected in the pellet cell fraction (82).
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Development of a small molecule that corrects misfolding and increases secretion of Z α1-antitrypsinbioRxiv - Pharmacology and Toxicology 2020Quote: ... Cells were pulsed (0.45MBq/106 cells) for 10 min with 35S Cys/Met (EasyTagTM Express Protein Labelling, Perkin Elmer, Beaconsfield, UK) in DMEM without Cys/Met ...
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bioRxiv - Microbiology 2021Quote: ... TZM-bl reporter cells were seeded at a density of 2 × 104 cells/well in 96-well luminometer-compatible tissue culture plates (PerkinElmer) 24 h before infection ...
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bioRxiv - Evolutionary Biology 2021Quote: ... with 15 cycles of PCR and using 1:125 dilution of NEXTflex DNA barcodes (Perkin Elmer,NOVA-514104). The resulting libraries were purified using the Agencourt AMPure XP system (Beckman Coulter ...
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bioRxiv - Biochemistry 2019Quote: ... The RNA 5’ phosphate was removed by quick dephosphorylation kit (New England biolabs) and phosphorylated by T4 polynucleotide kinase (New England biolabs) and [γ -32P]ATP (3000 Ci/mmol) (Perkin Elmer). The samples were loaded onto 10% polyacrylamide denaturing gel (8 M urea) ...
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bioRxiv - Molecular Biology 2020Quote: ... 5 µl of the virus or virus-antibody complex was added to each well in triplicates and allowed to incubate for 6 hrs before the cells were lysed with 50 µl/well of steadylite plus luciferase reagent (Perkin Elmer; 6066751). The luciferase activity was quantified using a BMG PHERAstar FS plate reader.
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bioRxiv - Microbiology 2021Quote: ... and Volocity 6 software (PerkinElmer Life Sciences, Boston, USA) was used to process images.
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bioRxiv - Cell Biology 2023Quote: ... containing 6 ml Ultima Gold scintillation cocktail (Perkin Elmer). Radiation in each sample was measured as counts/minute (CPM ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were then pulsed with 2.20 MBq per sample with Express Protein Labeling Mix (Perkin Elmer) and incubated for 2.5 ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were then labeled with 0.1 mCi/mL EasyTag EXPRESS 35S Protein Labeling Mix (Perkin Elmer) diluted in labeling media ...
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bioRxiv - Microbiology 2023Quote: ... Infected cells were lysed 2 days after infection with a britelite plus (PerkinElmer, Cat#6066769), and the luminescent signal was measured using a GloMax Explorer Multimode Microplate Reader (Promega).
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bioRxiv - Biochemistry 2020Quote: ... 50 µg/mL PEG20,000 (SERVA; Cat. # 33138), ATP (Sigma; Cat. # A-7699) (variable, corresponding to ATP-Km of the respective kinase, [33P]- γ-ATP (PerkinElmer; Cat. # NEG302H): approx ...
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bioRxiv - Neuroscience 2021Quote: NPCs were seeded onto poly-L-ornithine/laminin-coated 6-well and cyclic olefin co-polymer-based 96-well Cell Carrier Ultra microtiter plates (Perkin Elmer, Waltham, MA) at 1×105 and 2×103 cells/well ...
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bioRxiv - Cell Biology 2023Quote: ... or ECL anti-rabbit antibodies (NA934V; 17434242) (Amersham) were used for detection of probed proteins using enhanced chemiluminescence (ECL)(Perkin Elmer). Chemiluminescence was detected using an iBright FL1500 (ThermoFisherScientific ...
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bioRxiv - Immunology 2022Quote: ... Unspecific antibody binding on sorted cells was blocked with TSA blocking reagent (PerkinElmer) for 30 minutes ...
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bioRxiv - Biochemistry 2022Quote: ... 5 μL time points were taken and quenched with 1.33 mM EDTA then brought to 20 μL final volume for detection using 1 nM LANCE Ultra Europium anti-H3K4me1/2 antibody (TRF0402, PerkinElmer) and 50 nM LANCE Ultra Ulight-Streptavidin (TRF0102 ...
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bioRxiv - Biochemistry 2023Quote: ... FLAG and HA were detected using the LI-COR system and HRP-conjugated GAPDH primary antibody was incubated with blots for 2 hours at room temperature and detected with Western Lightning Plus-ECL (PerkinElmer) using a Bio-Rad Chemidoc System.
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... supernatants were collected and IL-6 assayed with AlphaLISA (PerkinElmer) using the manufacturer’s instructions.
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bioRxiv - Immunology 2019Quote: ... Cells were then distributed (80 µg of proteins per well) in a 96-well microplate (Optiplate, PerkinElmer). BRET2 between RLuc8 and GFP10 was measured 1 min after addition of 5 µM coelenterazine 400a/Deep blue C (Gentaur) ...
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bioRxiv - Cell Biology 2022Quote: ... For the host protein response screen 10,000 cells/well were seeded on 96 well microplates (Perkin Elmer, CellCarrier-96 Ultra Microplates ...
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bioRxiv - Biochemistry 2024Quote: ... Transformation of yeast cells (82) and in vivo labeling of iron-binding proteins with 55FeCl3 (Perkin Elmer) and immunoprecipitation of 55Fe/S proteins and scintillation counting were performed as published for yeast (59 ...
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Nonsense Mediated RNA Decay Is a Unique Vulnerability of Cancer Cells with SF3B1 and U2AF1 MutationsbioRxiv - Cell Biology 2021Quote: ... Activity of the SMG1i against other PI3 kinase family members (PI3Kα, PI3Kβ, PI3Kγ, PI3Kδ, MTOR) was evaluated using Amplified Luminescent Proximity Homogeneous Assay (Alpha) Screen assays (Perkin Elmer and Echelon Biosciences). PI3K or mTOR protein (produced in Sf9 cells ...
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bioRxiv - Biochemistry 2023Quote: ... 9-1-1 having an N-terminal 7-residue kinase recognition sequence on Mec3 was radiolabeled to a specific activity of approximately 70 cpm/fmol with [γ-32P] ATP (PerkinElmer Life Sciences, Inc) using the recombinant catalytic subunit of cAMP-dependent protein kinase produced in E ...
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bioRxiv - Microbiology 2020Quote: Plasmid DNA was sequenced on both strands by primer walking using the ABI PRISMDye Terminator Cycle Sequencing Ready Reaction kit (PerkinElmer) and an ABI PRISM 377 sequencer (Perkin-Elmer ...
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bioRxiv - Biochemistry 2020Quote: ... Base-line BRET signals were read immediately at 470 nm and 535 nm for 11 cycles using an EnVision instrument (PerkinElmer). Constant concentration of the agonist lorcaserin (4 µM ...
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bioRxiv - Molecular Biology 2021Quote: HDL-sRNA libraries were generated using the NEXTFlex Small RNA-Seq Kit v3 with 1:4 adapter dilution and 22 PCR cycles (PerkinElmer). Following PCR amplification ...
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bioRxiv - Immunology 2023Quote: ... The slides were subjected to sequential staining cycles as described before,3 although now automated using Opal 7-color Automation IHC Kit (NEL801001KT; PerkinElmer) on the BOND RX IHC & ISH Research Platform (Leica Biosystems ...
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bioRxiv - Molecular Biology 2023Quote: ... reagents and conditions of the BigDye Terminator v3.1 Cycle sequencing Kit and an automated capillary sequencer ABI 3700 DNA analyzer (Perkin Elmer, Applied Biosystems ...
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bioRxiv - Physiology 2020Quote: ... Cells were incubated with 50 μM 2DG containing 0.25 μCi 3H-2-deoxyglucose (2DG, Perkin Elmer) for 5 min and washed three times with cold PBS ...
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bioRxiv - Cell Biology 2021Quote: ... Horseradish peroxidase (HRP) conjugated secondary antibody was added in order for labelled proteins to be detected by Western Lightning Plus-ECL (PerkinElmer, 203-170071). Primary and secondary antibodies used are listed in Supplementary Table 5.
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... After 6-24 h at 37 °C (as specified in each figure legend) the cell medium was aspirated and substituted with serum-free cell medium containing [3H]thymidine (2 μCi/ml, Perkin Elmer, Waltham, MA). The incubation was continued for 30 min at 37 °C ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were incubated with mESC medium supplemented with 0.8 Mbq/mL of 35S Protein Labeling Mix (Perkin Elmer) at 37 °C with 5 % CO2 for 30 min ...
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bioRxiv - Molecular Biology 2019Quote: ... cells were metabolically labeled for 10 min with 10 μCi EasyTag™ EXPRESS35S Protein Labeling Mix (Perkin Elmer). Total cell lysate was harvested in RIPA buffer (70 ...
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bioRxiv - Microbiology 2020Quote: ... Protein localization in fungal cells was carried out by an UltraVIEW VoX Spinning Disk Confocal Microscope (PerkinElmer, USA) and a Leica DMi8 (Leica ...
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bioRxiv - Molecular Biology 2021Quote: ... the baseline BRET signals were read immediately at 470 nm and 535 nm for 10 cycles using an EnVision® multimode plate reader (PerkinElmer). Following the agonist addition ...
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bioRxiv - Biochemistry 2022Quote: ... and the NADH consumption was read at λ 340 nm during 120 cycles of 30 sec each by using a Victor multilabel plate reader 1420 Multilabel Counter (Perkin Elmer). In order to obtain catalytic rates and kinetic constants by Michaelis-Menten equation ...