Labshake search
Citations for PerkinElmer :
301 - 350 of 447 citations for Borrelia burgdorferi C t Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2022Quote: PDE activity was measured at 30°C with the two-step method described by [48] using [3H]cGMP (Perkin Elmer, MA, USA). Aliquots of eluted fraction from SEC were incubated in 60 mM HEPES pH 7.2 assay buffer containing 0.1 mM EGTA ...
-
bioRxiv - Neuroscience 2021Quote: Live-cell imaging was performed at 37°C and with 3% CO2 using an Opera Phenix™ high content confocal microscopy system (Perkin Elmer). One hour prior to imaging ...
-
bioRxiv - Immunology 2020Quote: ... The pyroptotic process was monitored at 34°C and 7.5% CO2 using a fully automated spinning disk confocal microscope (PerkinElmer Technologies, OPERA QEHS) with a 40x water immersion objective (Aulner et al. ...
-
bioRxiv - Immunology 2020Quote: ... and allowed to refold at 18°C for 24 h in a Streptavidin-coated scintillation microplate (Flashplate PLUS, Perkin Elmer, Boston, MA). Dissociation was initiated by adding excess unlabeled β2m and placing the microplate in a scintillation counter (TopCount NXT ...
-
bioRxiv - Plant Biology 2020Quote: ... The reaction was incubated for four hours at 37°C and the results were visualised using the LabChip GX Touch 24 (PerkinElmer, Waltham, USA).
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... The membrane mixture was then filtered over a 0.3% PEI pre-treated GF-B/C plate (#6005174, Perkin Elmer, Waltham, MA, USA) with a cell harvester system ...
-
bioRxiv - Immunology 2022Quote: ... and the reaction was continued for 24 hours at 37 °C before FP measurement using a Victor X5 Multilabel plate reader (PerkinElmer, Shelton, CT). DP4.1-Clip (250 nM) ...
-
bioRxiv - Microbiology 2023Quote: ... in 96-well plates in 28°C with shaking and the OD600 measurements were performed hourly using an EnVision Multilabel Reader (Perkin Elmer, USA).
-
bioRxiv - Cell Biology 2022Quote: ... Nascent proteins are labeled during translation reactions of 15-30 min at 32°C in RRL by incorporation of radioactive 35S-methionine (Perkin Elmer, USA). Nascent TA proteins were released from the ribosome with 1 mM puromycin and then incubated with 5% (v/v ...
-
bioRxiv - Immunology 2023Quote: ... Fluorescent signals were monitored in real-time with a microplate reader for 24 h within a tissue culture incubator (37 °C, 5 % CO2) on black OptiPlate 96-well (PerkinElmer, MA, USA) under 20x objective ...
-
bioRxiv - Molecular Biology 2024Quote: ... This RNA unwinding assay was performed at 37 °C in 384-well plates using a Victor Nivo plate reader (PerkinElmer, Basel, Switzerland). The excitation wavelength was set to 640 nM and the emission was measured at 685 nm ...
-
bioRxiv - Molecular Biology 2021Quote: Groups of five oocytes were incubated with 0.5 mCi/ml EasyTag EXPRESS 35S protein labeling mix (PerkinElmer, NEG772007MC) in 100 µl complete OR-2 buffer at 23°C for 1 hour ...
-
bioRxiv - Cell Biology 2022Quote: ... Cells were incubated with mESC medium supplemented with 0.8 Mbq/mL of 35S Protein Labeling Mix (Perkin Elmer) at 37 °C with 5 % CO2 for 30 min ...
-
bioRxiv - Molecular Biology 2019Quote: ... cells were metabolically labeled for 10 min with 10 μCi EasyTag™ EXPRESS35S Protein Labeling Mix (Perkin Elmer). Total cell lysate was harvested in RIPA buffer (70 ...
-
bioRxiv - Molecular Biology 2019Quote: ... and the separated proteins were electrophoretically transferred to a polyvinylidene difluoride (PVDF) membranes (NEF1002001PK, PerkinElmer, Waltham, MA, USA) by 400 mA for 75 minutes ...
-
bioRxiv - Microbiology 2019Quote: ... and a final concentration of 125 µMci/mL of EasyTag™ EXPRESS35S Protein Labeling Mix (Perkin Elmer, USA). Concentrations used for incubation correspond to 100- ...
-
bioRxiv - Neuroscience 2020Quote: ... The 5 μg of thawed S1 protein was radioactively labeled with 1 mCi 125I (Perkin Elmer, Waltman, MA) using the chloramine-T method 30 ...
-
bioRxiv - Biochemistry 2021Quote: Eluted protein was separated on an 8% polyacrylamide gel and transferred onto PolyScreen PVDF transfer membrane (Perkin Elmer). Membranes were blocked overnight in TBS containing 5% Bovine Serum Albumin (Sigma Aldrich) ...
-
bioRxiv - Biochemistry 2020Quote: ... Streptavidin Donor beads (Cat # 6760002) and Protein A acceptor beads (Cat # AL101) were acquired from PerkinElmer (Waltham, MA).
-
bioRxiv - Cancer Biology 2020Quote: ... The proteins were detected by western blot analysis by using an enhanced chemiluminescence system (Western Lightning– ECL, PerkinElmer). Antibodies used were specific for PTEN (Cell Signaling ...
-
bioRxiv - Biochemistry 2021Quote: ... 2020 submitted): namely in the presence of EasyTag EXPRESS 35S Protein Labelling Mix containing [35S] methionine (Perkin Elmer) (0.533 MBq ...
-
bioRxiv - Neuroscience 2022Quote: ... 25-30 μg of membrane protein was combined with a fixed concentration (0.57-5.18 nM) of 3H-diprenorphine (PerkinElmer) and concentration curves of KUNB106 or positive control (naloxone for mu and delta ...
-
bioRxiv - Microbiology 2020Quote: ... Protein localization in fungal cells was carried out by an UltraVIEW VoX Spinning Disk Confocal Microscope (PerkinElmer, USA) and a Leica DMi8 (Leica ...
-
bioRxiv - Cell Biology 2021Quote: ... Viral protein expression was detected by imaging with Opera LX and Phenix high content imaging platforms (Perkin Elmer) and the number of infected cells determined using a Columbus Analysis system (Perkin Elmer).
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... 30-40 μg of membrane protein was incubated with varying concentrations of compound and 0.3 nM [3H]-CP55,940 (PerkinElmer) for 90 min at room temperature ...
-
bioRxiv - Cell Biology 2020Quote: ... Extracts (15 μg protein) were electrophoresed on SDS-PAGE and then transferred to polyvinylidene difluoride membranes (Perkin Elmer). The membranes were incubated overnight at 40C on rocker with primary antibodies in 5% milk dissolved in 1x PBS tween ...
-
bioRxiv - Cancer Biology 2021Quote: Protein synthesis assays were performed by measurement of the incorporation of 3H-Leucine (Perkin Elmer, Waltham, MA USA) into protein ...
-
bioRxiv - Immunology 2023Quote: ... 10 µl of Protein A AlphaLISA® Acceptor beads (10 µg/ml final concentration; Perkin Elmer cat# AL101C) were added ...
-
bioRxiv - Physiology 2023Quote: ... Protein content was estimated using a diode array NIR spectrometer (DA-7200, Perten Instruments (today PerkinElmer, Waltham, USA)).
-
bioRxiv - Cancer Biology 2023Quote: ... BioRad) and protein bands were visualized by Western Lightning Plus ECL system (Catalog # ORT2655 and ORT2755, Perkin Elmer).
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... Protein-bound [3H]ryanodine was separated by filtration through polyethyleneimine-treated glass filters (Filtermat B; PerkinElmer, Waltham, MA) using a Micro 96 Cell Harvester (Skatron Instruments ...
-
bioRxiv - Biophysics 2023Quote: The ATPase activities of the protein samples were measured with a UV/Vis Spectrometer (Lambda 35, Perkin Elmer) using an ATP (2 mM ...
-
bioRxiv - Molecular Biology 2024Quote: ... anti-FLAG donor and protein G coated acceptor beads (10 ng/μl final concentration; AS103D and AL102C, PerkinElmer). For the competitive assay with CD81-GFP and antiHER2 EVs ...
-
bioRxiv - Microbiology 2021Quote: ... The mixture was incubated at 37 °C for 20 min before the RFU of fluorescent signals were read on an Envision microplate reader (Perkin Elmer, Waltham, MA) with excitation wavelength set at 320 nm and emission wavelength set at 400 nm ...
-
bioRxiv - Physiology 2020Quote: ... Progesterone was extracted with ethyl ether after overnight equilibration (4°C) of the plasma with 1500 dpm of tritiated progesterone (Perkin Elmer, Rodgau, Ermany). Separation of the organic and aqueous phase was conducted similar to the procedure described for the other hormones ...
-
Sequential dynein effectors regulate axonal autophagosome motility in a maturation-dependent pathwaybioRxiv - Cell Biology 2020Quote: ... All the videos (2 min, 4 frames s-1) were acquired at 37 °C using a Nikon TIRF microscopy system (Perkin Elmer, Waltham, MA) on an inverted Ti microscope equipped with a 100× objective and an ImageEM C9100-13 camera (Hamamatsu Photonics ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... Donor and acceptor emission were measured at 3 min intervals (during a total of 66 min) at 37°C with an EnVision 2014 Multilabel Reader (PerkinElmer, Waltham, MA, USA) using a 340/60 nm excitation filter and 520/8 nm (acceptor ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... lung tissues harvested from euthanized mice were soaked for 10 min in pre-warmed (37°C) PBS with 2% FBS and a 1:10 dilution of Xenolight RediJect Coelenterazine h (Cat# 706506, Perkin Elmer, Waltham, MA). Following a 12-15 min incubation ...
-
bioRxiv - Microbiology 2020Quote: ... Amastigotes were incubated at 37°C for 72 hours followed by lysis and measurement of luminescence using the ATPlite assay (Perkin Elmer, Waltham, MA).
-
bioRxiv - Cell Biology 2023Quote: All the videos (2 min, 4-5 frames s-1) were acquired at 37°C using a Nikon TIRF microscopy system (Perkin Elmer, Waltham, MA) on an inverted Ti microscope equipped with a 100× objective and an ImageEM C9100-13 camera (Hamamatsu Photonics ...
-
bioRxiv - Microbiology 2023Quote: ... was added to bacterial supernatants and the enzymatic activity was monitored for 30 min at 37°C (380 nm) in a VICTOR2 fluorometer/spectrophotometer (Perkin Elmer, Waltham, MA). The enzymatic activity ([PNA generated (nM ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... DPBS samples were diluted in 2% HNO3 and plasma samples were digested over night at 70°C in the 1:1 mixture of 70% HNO3 and H2O2 and analyzed using ICP-MS (Perkin Elmer NexION 300D) to determine the concentration of silver within each sample ...
-
bioRxiv - Biochemistry 2024Quote: ... 2B were prepared in ICM (Molsoft LLC., San Diego, CA). Chemical structures (Fig. 5A, C) were generated using ChemDraw (PerkinElmer informatics, Waltham, MA).
-
bioRxiv - Neuroscience 2021Quote: ... CHO-K1 cells stably expressing the luminescent Ca2+ indicator aequorin and the promiscuous Gα16 protein (ES-000-A24 cell line, PerkinElmer) were transiently transfected with ckr-1/pcDNA3.1 ...
-
bioRxiv - Immunology 2019Quote: ... Acquired images were used to build spectral library of all the target proteins using inForm Cell Analysis software (PerkinElmer).
-
bioRxiv - Biochemistry 2021Quote: ... Samples were prepared in a 96-well plate using the manufacturer “HT Protein Express Assay Sample Buffer” (PerkinElmer, USA) supplemented with 36.4 mM (final concentration ...
-
bioRxiv - Genomics 2021Quote: ... Percentage of protein content in the grain was measured using a Perten DA 7000 NIR analyzer (Perkin Elmer, Sweden).
-
bioRxiv - Microbiology 2021Quote: ... The final Western blotting bands were visualized using an enhanced chemiluminescence protein detection system (PerkinElmer Life Sciences, Boston, MA).
-
bioRxiv - Cancer Biology 2020Quote: ... shRNA expression with surrogate red fluorescent protein (RFP) was tracked by fluorescence (IVIS Spectrum In Vivo Imaging Station, PerkinElmer). For chloroquine xenograft experiments ...
-
bioRxiv - Cell Biology 2021Quote: ... Media were replaced with fresh media supplemented with Easytag Express S35 Protein Labeling Mix (PerkinElmer, 100-200 µCi/mL) for 1 h ...