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Citations for PerkinElmer :
201 - 237 of 237 citations for Anti PI3 kinase p150 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... involving the use of a biotinylated secondary antibody followed by an ABC complex and Cy3-tyramide kit (Perkin Elmer, cat#: FP1046) or an Alexa secondary antibody (1:500 ...
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bioRxiv - Bioengineering 2020Quote: ... Images are acquired by confocal microscopy to minimize auto-fluorescence of unlabeled dye/antibody, using a conventional laser scanning confocal microscope (CLSM510Meta, Zeiss) or a high-content screening system (Opera Phenix, Perkin Elmer). Instrument specifications are found in Supplemental Information ...
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bioRxiv - Cancer Biology 2020Quote: ... membranes were washed and probed with horseradish peroxidase (HRP)-conjugated secondary antibodies for 1 hour before detection with Western Lightning Chemiluminescence (Perkin Elmer). Antibodies used included ...
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bioRxiv - Cancer Biology 2021Quote: ... was used for antibody raised in rabbit (PH3 and PHGDH) and the OPAL Polymer HRP Ms+Rb (Akoya/Perkin Elmer, ARH1001EA) was used for the antibody raised in mouse (panCK) ...
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bioRxiv - Microbiology 2022Quote: ... and serum IgA antibodies to the secreted form of S-2P were measured by europium ion-enhanced DELFIA-TRF assay (Perkin Elmer) following the supplier’s protocol.
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bioRxiv - Neuroscience 2021Quote: ... and incubated with an anti-fluorescein horseradish peroxidase conjugate (1:500, PerkinElmer, Waltham, MA, USA) overnight at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... was captured on anti-mouse IgG (Fc specific) acceptor beads (Perkin Elmer, Cat nr. AL105C). Serial dilutions of antibodies and VHH-Fc (final concentration ranging between 100nM - 0,001 nM ...
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Development of a small molecule that corrects misfolding and increases secretion of Z α1-antitrypsinbioRxiv - Pharmacology and Toxicology 2020Quote: ... an anti-mouse IgG (1.5nM) labelled with fluorescence donor Eu-W1024 (Perkin Elmer product AD0076) and an anti-rabbit IgG (14.3nM labelled with acceptor (APC) ...
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bioRxiv - Neuroscience 2021Quote: ... The bound primary antibodies were detected by horseradish peroxidase-conjugated secondary antibodies in combination with an enhanced chemiluminescence detection system using Western Lightning Chemiluminescence Reagent (PerkinElmer Life Sciences) or ImmunoStar (Wako Pure Chemical Industries) ...
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bioRxiv - Neuroscience 2020Quote: ... Double immunofluorescence was performed using tetramethyl rhodamine labelled secondary antibody for alpha-synuclein and NSF with the fluorescein signal amplification kit (Perkin Elmer, UK). Following adequate washes to remove background fluorescence ...
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bioRxiv - Cell Biology 2021Quote: ... Horseradish peroxidase (HRP) conjugated secondary antibody was added in order for labelled proteins to be detected by Western Lightning Plus-ECL (PerkinElmer, 203-170071). Primary and secondary antibodies used are listed in Supplementary Table 5.
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bioRxiv - Cancer Biology 2023Quote: ... The phospho-peptide product was measured by time-resolved fluorescence resonance energy transfer (TR FRET) between the terbium of the antibody and fluorescein of the phospho-peptide using an EnVision plate reader (PerkinElmer, Waltham, MA) equipped to detect the TR-FRET signal (excitation 320 nm ...
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bioRxiv - Neuroscience 2020Quote: ... Cux2 (Atlas Antibodies, Stockholm, Sweden; rabbit, 1:200; detected with ABC kit [Vector Laboratories, Burlingame, CA, USA] and TSA [PerkinElmer, Waltham, MA, USA]), Dcx (Santa Cruz Biotechnology ...
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bioRxiv - Cancer Biology 2021Quote: ... Blots were stained with HRP anti-Rabbit IgG and developed with Western Lightning Plus-ECL (Perkin Elmer) on X-ray film.
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bioRxiv - Neuroscience 2022Quote: ... and then incubated in horseradish peroxidase (HRP)- conjugated anti-rabbit IgG (1:1,000; Perkin Elmer, Boston, MA) for 1.5 hours ...
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bioRxiv - Biophysics 2022Quote: ... Single-tethered DNA was stretched with blocking buffer containing 1 µg/mL biotinylated anti-digoxigenin (Perkin Elmer) at 100 µL/min flow rate ...
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Molecular basis of proteolytic cleavage regulation by the extracellular matrix receptor dystroglycanbioRxiv - Biochemistry 2024Quote: ... Samples were resuspended in Optimem with a 1:1000 dilution of APC conjugated to anti-Flag (PerkinElmer) and 1:500 dilution Alexa Flour 488 conjugated to anti-HA (Invitrogen ...
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bioRxiv - Cancer Biology 2020Quote: ... The membranes were incubated with horseradish peroxidase-conjugated IgG as a secondary antibody and the Western Lightning®-ECL system (PerkinElmer Inc., Waltham, MA) for detection.
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bioRxiv - Cancer Biology 2022Quote: ... filters were incubated for 1 hour at room temperature with alkaline phosphatase-coupled goat antirabbit antibodies and developed with Western Lightning Plus (Perkin Elmer, Inc., Waltham, MA) according to manufacturer directions.
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bioRxiv - Bioengineering 2023Quote: ... for SARS-CoV-2 S protein neutralization antibody titer in the wells was observed using the High Content Analysis System (Perkin Elmer, Waltham, MA, USA) or microscope ...
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bioRxiv - Neuroscience 2020Quote: ... the GlyT1 probe was detected using anti-DIG-HRP (1:750) and tyramide amplification (Perkin Elmer TSA Cy3). This was followed by the immunohistochemistry protocol to amplify the GFP signal in Dact2-GFP retinas ...
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bioRxiv - Neuroscience 2022Quote: ... sections were incubated in horseradish peroxidase-conjugated anti-rabbit IgG (1:1000; cat# NEF812001EA, Perkin Elmer, Boston, MA) for 1.5 hours ...
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bioRxiv - Molecular Biology 2024Quote: ... anti-FLAG donor and protein G coated acceptor beads (10 ng/μl final concentration; AS103D and AL102C, PerkinElmer). For the competitive assay with CD81-GFP and antiHER2 EVs ...
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bioRxiv - Neuroscience 2020Quote: ... slices were incubated in a goat anti-rabbit horse radish peroxidase-conjugated IgG (1:200, NEF812001EA, PerkinElmer, Waltham, MA) for 30 min ...
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bioRxiv - Genomics 2022Quote: ... the slides were washed three times in 1x TNT at RT for 5 minutes each and the amplification steps were repeated using anti-DNP-HRP Conjugate (PerkinElmer) and TSA Plus Cyanine 3 (PerkinElmer) ...
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bioRxiv - Genomics 2023Quote: ... blood was collected in EDTA anti-coagulant BD Vacutainer tubes and genomic DNA was extracted using the Chemagic MSM I Instrument (PerkinElmer) following the manufacturer’s recommendations and stored at −20°C before PCR 44 ...
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bioRxiv - Cell Biology 2023Quote: ... BMK-dko cells stably expressing a mCerulean3-fused anti-apoptotic protein were seeded at 4000 cells/well into a CellCarrier-384 Ultra Microplate (PerkinElmer). 24 hours later ...
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bioRxiv - Molecular Biology 2024Quote: ... were incubated with different concentrations of GST-hnRNPA2B1 (0-60 nM) in the presence of streptavidin Donor beads and anti-GST-Acceptor beads (PerkinElmer) (20 µg/ml final concentration) ...
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bioRxiv - Immunology 2022Quote: ... bound cytokines were detected by biotinylated anti-IFN-γ (clone 4s.B3) and quantified using a Victor2 D time-resolved fluorometer (Perkin Elmer). The % activity was calculated by dividing the SI of mutated peptides by the SI of the wild-type peptide ...
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bioRxiv - Cancer Biology 2019Quote: ... anti-mouse-HRP and anti-goat-HRP were used (1:2500, Amersham) and detection was done by chemiluminescence (Western Lightning Plus ECL, Perkin Elmer) using the Amersham Imager 600 (GE Healthcare).
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bioRxiv - Biophysics 2022Quote: ... The anti-puromycin fluorescence in the cytoplasm was detected automatically using the Opera Phenix® Plus High Content Screening System (PerkinElmer). The cytoplasm was detected automatically using the HARMONY v4.8 software.
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bioRxiv - Cancer Biology 2023Quote: ... HRP-coupled anti-mouse-HRP was used (1:2500, Amersham) and detection was done by chemiluminescence (Western Lightning Plus ECL, Perkin Elmer) using the Amersham Imager 600 (GE Healthcare).
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bioRxiv - Neuroscience 2019Quote: ... The secondary and Opal™ steps were then repeated using the anti-rabbit secondary and Opal™ 650 reagent (1:1000; PerkinElmer). Sections were then washed 3x for 2 min each in TBS-T and counterstained with DAPI to label nuclei.
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Wnt-dependent activation of ERK mediates repression of chondrocyte fate during calvarial developmentbioRxiv - Developmental Biology 2021Quote: ... The slides were then washed in 1X TBS-T and incubated in HRP-conjugated goat-anti-rabbit secondary (1:1500, PerkinElmer NEF812001EA, Akoya Biosciences) for 45 minutes ...
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bioRxiv - Neuroscience 2020Quote: ... Reaction termination and detection were performed using 1X LANCE detection buffer with 20 mM EDTA and 2 nM LANCE Ultra Europium anti-phospho Glycogen Synthase (Ser641) (Perkin Elmer #TRF0220-M).
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bioRxiv - Cell Biology 2021Quote: ... sections were washed in TBST and incubated with Alexa Fluor® 594 AffiniPure Goat Anti-Rabbit IgG (H+L) (Abacus DX, Australia) with Spectral DAPI (FP1490) added (1/1000) (Perkin Elmer, USA) for one hour at room temperature ...
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bioRxiv - Molecular Biology 2019Quote: ... Detection was carried out by incubating with a 1:5000 dilution of a goat anti-rabbit conjugated to peroxidase (Perkin Elmer and Sigma, Aldrich respectively). The latter were developed with the ECL Plus™ Western blotting detection system (PerkinElmer Life Sciences).