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Citations for PerkinElmer :
101 - 150 of 288 citations for Anti Cathepsin S Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Plates were read once every 30 s for 10 min at 37°C using a EnSpire Multimode Plate Reader (PerkinElmer) with excitation and emission wavelengths of 550 and 591 nm ...
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bioRxiv - Microbiology 2023Quote: ... purified 6×His-tagged SfTIR-STING was added to the reaction to a final assay volume of 50 μL and plates were read once every 15 s for 10 min at 37°C using a EnSpire Multimode Plate Reader (PerkinElmer) with excitation and emission wavelengths of 410 and 300 nm ...
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bioRxiv - Neuroscience 2019Quote: ... Primary antibodies were detected by incubation with peroxidase-conjugated anti-mouse and anti-rabbit antibodies (1:10000, Jackson Immuno Research Laboratories) followed by reaction with ECL-Plus reagent (Western Lighting, PerkinElmer) and exposure to autoradiography films ...
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bioRxiv - Neuroscience 2021Quote: ... and Lumi4-Tb cryptate conjugated anti-IP1 antibody (emission at 620 nm) was detected using an Envision plate reader (PerkinElmer). Results were calculated from the 665/620 nm ratio and normalised to the maximum response stimulated by acetylcholine.
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bioRxiv - Neuroscience 2022Quote: ... membranes were washed and incubated with the goat anti-rabbit IgG HRP-labeled secondary antibody (PerkinElmer, Waltham, MA, USA; NEF812001EA) for 1 h at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... anti-mouse or anti-rabbit secondary antibody (1:10000; Jackson Immuno Research Laboratories) followed by treatment with ECL-plus reagent (Western Lighting, PerkinElmer) and exposure to autoradiography films ...
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bioRxiv - Developmental Biology 2021Quote: ... Antisense RNA probes labeled with DNP were used in all in situ hybridizations and probe detection was performed using a HRP-coupled anti-DNP antibody (PerkinElmer) and a fluorescein coupled tyramide (Vize et al. ...
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bioRxiv - Microbiology 2022Quote: ... The membranes were then incubated with anti-IgG rabbit antibodies conjugated to horseradish peroxidase (PerkinElmer Life Sciences (Boston, MA, USA), and developed using the Western Lightning Chemiluminescence Reagent Plus (PerkinElmer) ...
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bioRxiv - Neuroscience 2022Quote: ... then incubated for 30 min at 25 °C with shaking at 400 RPM with 9 ng/well of DELFIA Eu-N1 anti-mouse antibody in DELFIA assay buffer (PerkinElmer), washed with 3 × 100 μl of PBST before enhancing with 80 μl of DELFIA Enhancement Solution (PerkinElmer) ...
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bioRxiv - Biochemistry 2022Quote: ... 5 μL time points were taken and quenched with 1.33 mM EDTA then brought to 20 μL final volume for detection using 1 nM LANCE Ultra Europium anti-H3K4me1/2 antibody (TRF0402, PerkinElmer) and 50 nM LANCE Ultra Ulight-Streptavidin (TRF0102 ...
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bioRxiv - Bioengineering 2019Quote: ... Radiance values for ICG (p/s/cm2/sr) were measured from each ROI using LivingImage software (PerkinElmer, Waltham, Massachusetts, United States). Following FLI ...
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bioRxiv - Neuroscience 2021Quote: ... [35S]-GTPγS (1250 Ci/mmol) and [5,8-3H]-adenosine 3,5-cyclic phosphate (34.0 Ci/mmol) were from Perkin Elmer (Boston, MA, USA).
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bioRxiv - Molecular Biology 2022Quote: ... 1mM PMSF, 100mM KCl and 1 mCi of [3H]AdoMet (S-adenosyl-L-[methyl-3H] methionine, 80 Ci/mmol; SAM) (Perkin Elmer) or 0.15mM unlabeled S-adenosyl methionine for 45 min at 30°C ...
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bioRxiv - Microbiology 2022Quote: ... Cytotoxicity was measured by adding resazurin [alamar blue (ThermoFisher)] diluted to 1x in DMEM according to the manufacturor’s protocol and incubated on cells for 2-4hr and reading fluorescence on a Victor 1420 Multilabel plate reader (Perkin Elmer) with excitation at 560 nM and emission at 590 nM.
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bioRxiv - Molecular Biology 2020Quote: Purified protein complexes were incubated at room temperature with a histone substrate at a final concentration of 500nM and approximately 500nM radioactive SAM (Adenosyl-L-methionine S-methyl3H from PerkinElmer (NET155H250UC)) in methyltransferase buffer (10% glycerol ...
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bioRxiv - Molecular Biology 2023Quote: ... were cultured at 6000 cells per well in DMEM (+10% FBS, +1% P/S) on Perkin Elmer LLC CellCarrier-96 Ultra Microplates (Perkin Elmer) coated with PLO overnight ...
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bioRxiv - Biochemistry 2023Quote: ... Reactions are incubated at 30 °C for 20 min prior to luminescence measurement for 10 s on the Victor X5 Multilabel Plate Reader (Perkin Elmer) by addition of 75 μl Renilla luciferase substrate (Promega) ...
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bioRxiv - Immunology 2021Quote: ... Cells were immunostained with DAPI and Alexa488-conjugated anti-N antibody (CR3009; produced in-house) and imaged using an Opera Phenix (Perkin Elmer). The ratio of infected (Alexa488+ ...
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bioRxiv - Physiology 2019Quote: ... Cells were incubated with an anti-ERα antibody (F-10) overnight followed by Cy3 Tyramide signal amplification (PerkinElmer-TSA-Plus-Cy3) according to manufacturer’s instructions ...
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bioRxiv - Biophysics 2019Quote: AlphaScreen-based peptide recruitments assays were performed using streptavidin coated donor beads and anti-His-tag antibody coated AlphaLisa acceptor beads (Perkin Elmer). The assays were very sensitive to the distance of the N-terminus to the LXXLL NR-box motif and the use of a flexible Ahx linker ...
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bioRxiv - Genomics 2021Quote: ... the anti-DIG-HRP antibody was detected with the TSA-Cy3 substrate (1:50, TSATM PLUS Cy3 kit, NEL 745001KT, Perkin Elmer) for 10 min at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... The membrane was probed with a goat anti-mouse antibody conjugated to horseradish peroxidase and bands were visualized using the Western Lighting Plus-ECL reagent (Perkin Elmer). When performing the KatG expression analysis in response to C10 treatment ...
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bioRxiv - Cell Biology 2023Quote: ... or ECL anti-rabbit antibodies (NA934V; 17434242) (Amersham) were used for detection of probed proteins using enhanced chemiluminescence (ECL)(Perkin Elmer). Chemiluminescence was detected using an iBright FL1500 (ThermoFisherScientific ...
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bioRxiv - Bioengineering 2022Quote: ... Average radiance values in p/s/cm2/sr were measured from each well using Living Image software (PerkinElmer, Waltham, Massachusetts, United States).
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bioRxiv - Molecular Biology 2023Quote: ... were measured using a radiometric assay that detects transfer of a [3H] methyl from the radio-labeled cofactor S-[Methyl-3H]-adenosyl-L-methionine (SAM, PerkinElmer, cat. NET155001MC) to a biotinylated peptide Akt1-K64 (QCQLMKTERPRPNTFIIRCLQW ...
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bioRxiv - Bioengineering 2022Quote: ... PEG-maleimide was reacted with 1 mol equivalence of calpain substrate peptide and 1 mol equivalence of L-cysteine-functionalized VivoTag S-750 (PerkinElmer, Boston, MA), followed by quenching with an excess of L-cysteine ...
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bioRxiv - Molecular Biology 2023Quote: DNA methylation of replicating sperm or erythrocyte nuclei in egg extract was assayed by the incorporation of 3H-SAM (S-[methyl-3H]-adenosyl-L-methionine; Perkin Elmer, NET155H). Demembranated sperm nuclei were prepared as published previously (86) ...
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bioRxiv - Neuroscience 2019Quote: ... The sections were incubated overnight at 4ºC with horseradish peroxidase-conjugated anti-fluorescein antibody (diluted 1:1000 and 1:500 for detection in PMm/PMg and other nuclei, respectively; PerkinElmer, Waltham, MA) and Dylight 549-conjugated goat anti-rabbit IgG (diluted 1:500 ...
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bioRxiv - Neuroscience 2021Quote: ... the sections were incubated with a horseradish peroxidase-conjugated anti-fluorescein antibody (diluted 1:500 with DIG-1, PerkinElmer, Foster City, CA) for 1 h and washed twice with DIG-1 for 5 min each time ...
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bioRxiv - Biochemistry 2022Quote: ... Detection reagents were added stepwise with 30 min incubation of anti-H3K4me3 antibody and Ulight-Streptavidin with reaction time points followed by 1 hr incubation with Europium anti-rabbit antibody in 384 well white microplates (PerkinElmer OptiPlate-384). TR-FRET emission at 665 nm and at 615 nm by 320 nm excitation with 50 μs delay and 100 μs integration time was measured using a Molecular Devices SpectraMax M5e plate reader ...
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bioRxiv - Immunology 2024Quote: ... Five-micrometer serial sections were stained with a human-specific anti-COXIV antibody and scanned using the Vectra® Polaris Automated Imaging System (PerkinElmer, USA) at 20×magnification ...
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bioRxiv - Cell Biology 2022Quote: ... Luminescence was measured at 1 h post PathHunter© detection reagent addition using Victor3 plate reader (Perkin Elmer 0.5-s/well integration time). Data were normalized to control treated with the highest concentration of ligand (100%) ...
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Sequential dynein effectors regulate axonal autophagosome motility in a maturation-dependent pathwaybioRxiv - Cell Biology 2020Quote: ... All the videos (2 min, 4 frames s-1) were acquired at 37 °C using a Nikon TIRF microscopy system (Perkin Elmer, Waltham, MA) on an inverted Ti microscope equipped with a 100× objective and an ImageEM C9100-13 camera (Hamamatsu Photonics ...
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bioRxiv - Biochemistry 2022Quote: ... and the change in tryptophan fluorescence associated with the exchange of guanyl nucleotides within the α subunit of Gt was measured for 1200 s with a FL 6500 Fluorescence Spectrometer (PerkinElmer, Waltham, MA). Excitation and emission wavelengths were set at 300 nm and 345 nm ...
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bioRxiv - Cancer Biology 2019Quote: pWPXLD-3xflag-shank2 (5 μg) was incubated with GST-PRMT7 in the presence of S-adenosyl-methionine (SAM; 15 Ci/mmoL; PerkinElmer, Waltham, MA, USA) at 30°C for 1 h ...
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bioRxiv - Cell Biology 2023Quote: All the videos (2 min, 4-5 frames s-1) were acquired at 37°C using a Nikon TIRF microscopy system (Perkin Elmer, Waltham, MA) on an inverted Ti microscope equipped with a 100× objective and an ImageEM C9100-13 camera (Hamamatsu Photonics ...
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bioRxiv - Neuroscience 2023Quote: ... Reactions were initiated by adding 2.5 µM ATP at 27°C and were terminated after 60 min with 10 µL of 2.5X Stop Solution/Detection Mix containing 20mM EDTA and 4nM LANCE Ultra Europium-anti-phospho-PKC (Ala25Ser) peptide antibody (Perkin Elmer, Waltham, MA). After incubation at 27°C for another 60 min ...
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bioRxiv - Developmental Biology 2020Quote: Recombinant GST-SRF protein or synthetic SRF peptides were incubated for 3 h at 30 °C with GST-SET7 in the presence of 100 nCi of S-adenosyl-[methyl-14C]-L-methionine [14C-SAM] (Perkin Elmer, Waltham, MA, USA) in HMTase assay buffer (50 mM Tris–HCl [pH 8.5] ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 5 μL of 1/100 dilution of the Alexa Fluor® 647-anti cAMP antibody solution in the Detection Buffer (PerkinElmer Life and Analytical Sciences) and 10 μL of 1/5000 dilution of Eu-W8044 labeled streptavidin and Biotin-cAMP in the Detection Buffer (PerkinElmer Life and Analytical Sciences ...
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bioRxiv - Biochemistry 2021Quote: ... 5 μL of 1/100 dilution of the Alexa Fluor® 647-anti cAMP antibody solution in the Detection Buffer (PerkinElmer Life and Analytical Sciences) and 10 μL of 1/5000 dilution of Eu-W8044 labeled streptavidin and Biotin-cAMP in the Detection Buffer (PerkinElmer Life and Analytical Sciences ...
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bioRxiv - Cell Biology 2021Quote: ... Images were acquired for 60 min at 1 frame/15-30 s using a spinning disk confocal microscope (UltraVIEW VoX, PerkinElmer with a 63X 1.4 N.A. oil objective). Images were analyzed using Volocity Suite (PerkinElmer ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were imaged at 1 frame/30 s for 4 h at 37°C with 5% CO2 using a spinning disk confocal microscope (UltraVIEW VoX, PerkinElmer with a 63X 1.4 N.A. oil objective), followed by addition of 50 μg/ml PI (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... Primary antibodies were detected using HRP-linked secondary antibodies together with Western Lightning Plus-ECL (Perkin Elmer). Fiji (ImageJ ...
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bioRxiv - Neuroscience 2020Quote: ... antibodies plus Cy3-TSA (1:200, PerkinElmer). Sections were cover-slipped using Prolong Diamond Antifade Mounting media with DAPI (ThermoFisher ...
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bioRxiv - Cell Biology 2023Quote: ... Antibodies were detected via ECL reagents (PerkinElmer).
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bioRxiv - Cancer Biology 2024Quote: ... blocked in antibody (Ab) Diluent (Perkin Elmer), incubated for 30 min with the primary antibody ...
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bioRxiv - Developmental Biology 2019Quote: ... or a tyramine-amplified fluorescent antibody (Perkin Elmer).
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bioRxiv - Microbiology 2020Quote: A matrix with all antibodies provided by PerkinElmer was tested for the ability to detect NP.
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bioRxiv - Neuroscience 2024Quote: ... and blocked in antibody diluent (PerkinElmer, Waltham, MA). Sections were then sequentially stained for Iba-1 (1:500 ...
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bioRxiv - Neuroscience 2020Quote: ... All antibodies were diluted in TNB solution from PerkinElmer. Sections were then incubated for 30 min in streptavidin-horseradish peroxidase that was diluted (1:200 ...