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Citations for PerkinElmer :
201 - 250 of 381 citations for Acetylated Lysine Rabbit Polyclonal HRP labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... The signals were detected by HRP-conjugated secondary antibodies and ECL reagent (PerkinElmer). Quantitative Western blotting was made using ImageJ ...
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bioRxiv - Cell Biology 2023Quote: ... HRP-conjugated antibodies were detected using western lighting plus-ECL reagent (Perkin elmer).
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bioRxiv - Cancer Biology 2019Quote: 1000 U2OS-KDM4B expressing cells in 25 μl of media were plated into each well of a poly-D-lysine coated Perkin Elmer 384-well View plates (Perkin Elmer 6007710) with a Thermo Scientific Wellmate ...
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bioRxiv - Molecular Biology 2021Quote: ... metabolically labeled for 20 min with 35S-Met (50 μCi 35S-Met, 1175 Ci/mmol, Perkin Elmer) and then lysed in SDS-lysis buffer (25 mM Tris-HCl pH 7.5 ...
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bioRxiv - Genomics 2020Quote: ... at 37°C for 1 hr and then labeled with 0.5μl of gamma 32P-ATP (PerkinElmer, BLU502A250UC) using 20U T4 polynucleotide kinase (NEB ...
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Transcription factor LSF facilitiates lysine methylation of α-tubulin by microtubule-associated SET8bioRxiv - Biochemistry 2019Quote: ... or recombinant MBP-β-tubulin were incubated with 6 μM radioactively labeled [3H] AdoMet (Perkin Elmer #NET155V001MC) in 1X HMT buffer containing 5 mM Tris pH 8.0 ...
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bioRxiv - Cancer Biology 2020Quote: Imaging of fluorescently labeled tissue sections was performed utilizing Nuance-FX multispectral imaging system (Caliper LifeSciences, PerkinElmer), using full capabilities of its Tunable Liquid Crystal imaging module ...
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bioRxiv - Biochemistry 2021Quote: ... 100 pmol of RNA were 5’-end labeled with [γ- 32P]-ATP (3000 Ci/mmol, Perkin Elmer) using T4 polynucleotide kinase (NEB ...
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bioRxiv - Molecular Biology 2022Quote: ... 10mM sodium butyrate and 0.25 µCi/µl (4.9 Ci/mmol) of [3H]-labeled Acetyl-CoA (Perkin Elmer) or 0.15mM unlabeled Acetyl-CoA (Sigma) ...
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bioRxiv - Molecular Biology 2022Quote: DNA probes (U1-SH, U4-14B, and U6-SH) were end labeled with [γ-32P]-ATP (PerkinElmer) using T4 PNK (Thermo Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... Riboprobes were labeled with either digoxigenin-UTP or dinitrophenyl-11-UTP (DNP; Perkin Elmer, Waltham, MA, USA). A DNP-labeled probe and a DIG-labeled probe were hybridized simultaneously ...
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bioRxiv - Microbiology 2019Quote: ... while the 14C-labeled methyladenosines were detected with a Packard radiomatic 515TR flow scintillation analyzer (Perkin Elmer).
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bioRxiv - Microbiology 2019Quote: ... was prepared and labeled according to manufacturer’s guidelines using [gamma- 32P]-ATP (6,000 Ci/mmol) (Perkin Elmer) and T4 Polynucleotide Kinase (PNK ...
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bioRxiv - Biophysics 2021Quote: ... The primer was labeled on its 5’end with (γ 32P)-ATP (Perkin Elmer, 3000 Ci.mmol−1) and T4 polynucleotide kinase (Thermofisher) ...
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bioRxiv - Genomics 2021Quote: ... Double-stranded DNA probes were prepared by end-point PCR and labeled with dCTP [α-32P] (PerkinElmer) using the Cytvia Amersham Megaprimer labeling kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... and Tris buffer containing trace amount of 32P-labeled orthophosphoric acid (∼1.2 pmol and ∼1 µCi, PerkinElmer) and different concentrations of cold phosphate (NaH2PO4 ...
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bioRxiv - Genetics 2020Quote: ... DNA methylation was measured by the incorporation of tritiated methyl groups from radioactively labeled SAM (Perkin Elmer) into the biotinylated substrate using an avidin–biotin methylation plate assay47 ...
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bioRxiv - Plant Biology 2022Quote: ... The membranes were UV cross-linked and hybridized with miRNA probes labeled with [γ-32P] ATP (PerkinElmer), with U6 served as loading control ...
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bioRxiv - Neuroscience 2023Quote: ... Slides were then placed in a tracer buffer containing 1-125-labeled OVTA ligand (Perkin Elmer NEX2540) for 60 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... and cells were incubated for 5 min before addition of 200μCi 35S-labeled methionine/cysteine mixture (PerkinElmer) and incubation for 1 hr at 37°C ...
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bioRxiv - Immunology 2023Quote: ... cells were labeled with 0.5 uCi of 56 mCi/mmol [14C] acetic acid (Perkin Elmer Cat# NEC084H001MC) per well overnight ...
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bioRxiv - Genetics 2021Quote: ... 2.5 µl of fixed and stained cells were transferred from 96-well plates into a poly-lysine-coated 384-well Perkin Elmer Cell Carrier Ultra imaging plate (PerkinElmer, Cat. no. 6057500), pre-filled with 25 µl of 1µM SYTOX Green using a Biomek Fx robot ...
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bioRxiv - Molecular Biology 2019Quote: ... cells were metabolically labeled for 10 min with 10 μCi EasyTag™ EXPRESS35S Protein Labeling Mix (Perkin Elmer). Total cell lysate was harvested in RIPA buffer (70 ...
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bioRxiv - Neuroscience 2020Quote: ... The 5 μg of thawed S1 protein was radioactively labeled with 1 mCi 125I (Perkin Elmer, Waltman, MA) using the chloramine-T method 30 ...
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bioRxiv - Immunology 2020Quote: ... The integrated signal density corresponding to labeled antigen or antibody in each LN was calculated using LivingImage (PerkinElmer) or ImageJ software and plotted using GraphPad Prism 8 ...
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bioRxiv - Microbiology 2020Quote: ... Cultures were grown until OD600 ∼ 0.02 and labeled with 50 μCi/ml 32P-orthophosphate (900 mCi/mmol; PerkinElmer). When OD600 reached 0.2-0.4 ...
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bioRxiv - Microbiology 2020Quote: ... was used for hybridization with oligonucleotides 5’-end labeled with 6,000 Ci/mmol γ-32P ATP (Perkin Elmer) using T4 polynucleotide kinase (NEB) ...
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bioRxiv - Biochemistry 2023Quote: ... the beads were suspended in 25 µl of cytoplasmic buffer containing 2 µCi of 3H-labeled SAM (PerkinElmer) and incubated overnight at 32 °C without shaking ...
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bioRxiv - Cell Biology 2023Quote: ... cells were labeled using an 35S Met/Cys labeling mixture at 200 µCi/ml (EasyTag Express35S; Perkin Elmer) for 30 minutes in media that contained drug treatments and that was 20 percent MEF culture medium as above and 80 percent DMEM lacking Met/Cys and with dialyzed FBS ...
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bioRxiv - Cell Biology 2020Quote: ... HA-binding protein staining was achieved with 1/100 strepavidin HRP (Perkin Elmer, Massachusetts, USA) solution for 30 min at room temperature and 3,3’-diaminobenzidine (Liquid DAB + substrate Chromagen System ...
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bioRxiv - Neuroscience 2022Quote: ... Secondary anti-HRP conjugate antibodies were used and immunoreactivity was visualized using Western LightingTM (PerkinElmer). Immunoreactivity was developed on film or using a ChemiDoc™ imaging system (Biorad) ...
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bioRxiv - Microbiology 2020Quote: ... programmed with 1 µg DNA/50 µL and labeled with 20 µCi of L-[35S]-methionine (EasyTag, Perkin Elmer). Reactions were incubated at 30 °C for 30 min before adding L-methionine (1 mM final ...
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bioRxiv - Biochemistry 2019Quote: ... Oligonucleotides were 5’-end-labeled with [γ-32P]-ATP (10 mCi ml-1, 3000 Ci mmol-1; Perkin Elmer) using polynucleotide kinase (Thermo Scientific) ...
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bioRxiv - Microbiology 2019Quote: ... primer (5’-AGUUGUUGAUC) was 32P-labeled at the 5′ position upon incubation with [γ-32P] ATP (PerkinElmer, Waltham, MA) and T4 polynucleotide kinase (NEB ...
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bioRxiv - Biophysics 2020Quote: ... supplemented with canine rough microsomes (tRNA probes, College Station, TX) and EasyTag 35 S-labeled methionine (PerkinElmer, Waltham, MA) for one hour at 30 °C ...
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bioRxiv - Cell Biology 2022Quote: ... The cells were labeled with 250-1000 µCi/ml of [35S]-Express label (1100 Ci/mmol; PerkinElmer, Boston, MA) at 37°C depending on the length of the pulse ...
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bioRxiv - Immunology 2021Quote: ... The 3H-labeled products were isolated using DEAE-Sephadex A-50 columns and quantitated by liquid scintillation counting (PerkinElmer). Non-isotopic methylated RNA fragments were prepared using cold SAM (Biolabs ...
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bioRxiv - Microbiology 2022Quote: ... cells were metabolically labeled for 16 h with 100 μCi/mL [35S] methionine-cysteine ([35S] protein labeling mix; PerkinElmer) in Dulbecco’s modified Eagle’s medium lacking methionine and cysteine and supplemented with 10% of dialyzed fetal bovine serum and 1X GlutaMAX™ (ThermoFisher Scientific) ...
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bioRxiv - Microbiology 2022Quote: ... Extracted crRNAs were end-labeled with T4 Polynucleotide Kinase in a reaction containing γ-[32P]-ATP (PerkinElmer, MA, USA), and resolved on an 8% Urea PAGE ...
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bioRxiv - Molecular Biology 2023Quote: ... Purified RNA oligos were either phosphorylated with unlabeled ATP or 5’ end-labeled using [γ-32P] ATP (Perkin Elmer) and T4-Polynucleotide kinase (New England Biolabs) ...
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bioRxiv - Biophysics 2023Quote: ... CFTR variants were pulse-labeled with 0.1-0.2 mCi/ml 35S-methionine and 35S-cysteine (Easy Tag Express, Perkin Elmer) in Cys- and Met-free DMEM (Gibco™ 21013024 ...
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bioRxiv - Molecular Biology 2024Quote: ... from synthetic oligonucleotides (0.2 µM) and trace-labeled with 32P alpha-ATP (800 Ci/mmol, 12.5 µM, Perkin Elmer) in a 20 µl reaction according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... TSA Plus HRP-Fluorescence kits were used for both Cy3 and Cy5 fluorescence channels (PerkinElmer NEL752001KT). For simultaneous SOX9 immunofluorescence ...
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bioRxiv - Cancer Biology 2019Quote: ... After incubation in HRP-linked secondary antibody followed by ECL reagent (Western Lightning Plus-ECL (PerkinElmer) or SuperSignal West Femto (ThermoFisher Scientific) ...
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bioRxiv - Immunology 2022Quote: ... the primary antibody was detected using Opal Polymer HRP Ms + Rb (PerkinElmer Opal Multicolor IHC system). The PerkinElmer Opal Multicolor IHC system was utilized to visualize antibody staining per manufacturer protocol ...
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bioRxiv - Cell Biology 2020Quote: ... and labeled for 5 min with with 1 μL per OD of cells of EXPRESS 35S Protein Labeling Mix (PerkinElmer) for 5 or 10 min ...
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bioRxiv - Genomics 2019Quote: ... FISH experiments were performed using human fosmids containing non-duplicated region directly labeled by nick-translation with Cy3-dUTP (PerkinElmer), Cy5-dUTP (PerkinElmer) ...
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bioRxiv - Biochemistry 2019Quote: RAW264.7 cells in 24-well plates were labeled by overnight incubation with DMEM containing 1% fetal bovine serum and 0.5 μCi/mL [3H]cholesterol (Perkin Elmer). The next day ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were then treated with a mixture of [3H]-labeled steroids (final concentration, 10 nM T and 10 nM progesterone; ~1,000,000 cpm/well; PerkinElmer, Waltham, MA) at 37 °C ...
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bioRxiv - Biochemistry 2020Quote: ... newly synthesized proteins in the cells were labeled by treating with 150 μCi/ml of 35S-methionine/cysteine (EXPRE35S35S Protein Labeling Mix, NEG772, PerkinElmer) apically for 45 min (pulse labeling) ...