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Citations for PerkinElmer :
51 - 100 of 109 citations for 8 Cyano octanoic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2024Quote: ... Total nucleic acid was extracted from the tissues using the Chemagic 360 instrument (PerkinElmer) and quantified with Qubit DNA High Sensitivity Kit (Catalogue Number ...
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bioRxiv - Immunology 2021Quote: ... with the last 8 hours in the presence of [3H] thymidine (1 mCi/well, NET027001MC; PerkinElmer). Incorporated [3H] thymidine was counted by a BetaPlate scintillation counter (Wallac ...
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bioRxiv - Biochemistry 2023Quote: ... in the presence of 20 µM of the corresponding L-[14C]-amino acid (Perkin Elmer). AaRSs were diluted in 100 mM HEPES-KOH pH 7.5 ...
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bioRxiv - Cancer Biology 2021Quote: ... Signal detection was performed by 8 min incubation with the PerkinElmer TSA Plus Cyanine 3 kit (PerkinElmer, NEL744E001KT ...
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bioRxiv - Biochemistry 2021Quote: Eluted protein was separated on an 8% polyacrylamide gel and transferred onto PolyScreen PVDF transfer membrane (Perkin Elmer). Membranes were blocked overnight in TBS containing 5% Bovine Serum Albumin (Sigma Aldrich) ...
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bioRxiv - Cell Biology 2022Quote: ... the mice were subjected to inhalational anesthesia using an XGI-8 Gas Anesthesia System (PerkinElmer, Waltham, MA, USA) containing a mixture of 4% isoflurane (ISOTROY 100 ...
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bioRxiv - Genomics 2020Quote: ... 3 times 5 min washes with TNT and signal amplification for 8 min using Cy3 (NEL704A001KT, Perkin Elmer) 1:50 in amplification diluent ...
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bioRxiv - Microbiology 2020Quote: ... 100 μCi ml−1 [32P] phosphoric acid added to label newly synthesized RNA (Perkin Elmer #NEX053H). RNA was harvested in Trizol (Thermofisher #15596018 ...
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bioRxiv - Biochemistry 2019Quote: ... 100 mM acetic acid (Roth) and 0.1% Triton X-100) using a plate reader (Victor3V, PerkinElmer) with excitation and emission wavelengths of 340 nm and 614 nm ...
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bioRxiv - Physiology 2020Quote: ... to be read using a LabChip GX Touch Nucleic Acid Analyser (Perkin Elmer, Cat. No. CLS138162). Reverse transcription and amplification of RNA purified from isolated cells was performed with the NuGEN Ovation RNA-Seq System V2 (NuGEN Cat ...
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bioRxiv - Biochemistry 2023Quote: ... The samples were then separated on a HayeSep column (7’ HayeSep N 1/8’’ Sf; PerkinElmer Inc., Waltham, USA), followed by molecular sieve (9’ Molecular Sieve 13x 1/8’’ Sf ...
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DIFFERENTIAL BIOENERGETICS IN ADULT RAT CARDIOMYOCYTES ISOLATED FROM THE RIGHT VERSUS LEFT VENTRICLEbioRxiv - Cell Biology 2020Quote: Fatty acid oxidation was measured using freshly isolated cardiomyocytes incubated in suspension with 14C-palmitate (Perkin Elmer) conjugated to BSA ...
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bioRxiv - Microbiology 2021Quote: ... and Tris buffer containing trace amount of 32P-labeled orthophosphoric acid (∼1.2 pmol and ∼1 µCi, PerkinElmer) and different concentrations of cold phosphate (NaH2PO4 ...
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bioRxiv - Molecular Biology 2021Quote: FA β-oxidation were measured by the conversion of [9,10-3H(N)]-Palmitic Acid (PerkinElmer, Waltham, MA) to 3H2O ...
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bioRxiv - Microbiology 2023Quote: ... An aliquot of the supernatant was processed for total nucleic-acid extraction using Chemagic 360 (Perkin Elmer). Nucleic acid preparations from wastewater samples are known to contain PCR inhibitors that interfere with their accurate quantification using PCR-based methods ...
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bioRxiv - Immunology 2022Quote: ... the reaction was stopped with 1M phosphoric acid and OD450 was measured by multimode microplate reader (PerkinElmer EnVision 2105 ...
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bioRxiv - Immunology 2023Quote: ... cells were labeled with 0.5 uCi of 56 mCi/mmol [14C] acetic acid (Perkin Elmer Cat# NEC084H001MC) per well overnight ...
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bioRxiv - Microbiology 2022Quote: ... was phosphorylated in vitro with His-MBP-PknBMtb at 0.015 μg/μl for 30 minutes at room temperature with 0.15μCi/μl ATP [γ-32P] (5×10-8 μmol) (3000Ci/mmol, 10mCi/ml, PerkinElmer BLU002A250UC) and 2mM MnCl2 in buffer (50mM Tris ...
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bioRxiv - Microbiology 2020Quote: ... Uptake was measured at room temperature by mixing 100 µl of cells with 100 µl of transport buffer containing 8 µM of [14C] glycerol (0.125 µCi each in triplicates, PerkinElmer Inc.). Incorporation of radioactivity was determined as described above.
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bioRxiv - Cancer Biology 2024Quote: ... was calculated for each mouse using a circular region of interest with the mouse in a prone position and normalized to the value obtained at the same area before starting treatment administrations (day 8) using the IVIS® Spectrum Living Image® 4.3.1 software (PerkinElmer). Tumor growth was also monitored thrice a week from day 15 by measuring tumor volume with a Vernier caliper using the modified ellipsoid volume formula ...
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bioRxiv - Pathology 2023Quote: ... double immunohistochemistry for paraffin sections (8 μm) was conducted by using the Opal 4 color manual immunohistochemistry (IHC) kit (NEL810001KT, PerkinElmer). The signal of p-MLC2 was amplified by the Opal 4 color manual IHC kit (NEL810001KT ...
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bioRxiv - Neuroscience 2024Quote: ... 100 µL of cell homogenate (0.05 mg protein/well) and 100 µL of [3H]8-OH-DPAT (0.5 nM final concentration, 170 Ci/mmol, Perkin Elmer) in a final volume of 1 mL ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were then changed to 600 μl medium containing 1 μCi [9,10(n)-3H]palmitic acid (Perkin Elmer) and 1 mM carnitine for 2 hours ...
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bioRxiv - Plant Biology 2022Quote: ... dissolved in 0.1% nitric acid and 4 mL Optima Gold XR scintillant (Perkin Elmer, Glen Waverley, VIC, Australia) was added prior to counting for two minutes using a Liquid Scintillation Counter (LS6500 ...
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bioRxiv - Cancer Biology 2023Quote: ... Total nucleic acid was extracted from FFPE tissue sections using Chemagic 360 sample-specific extraction kits (Perkin Elmer). Percent tumor cellularity as a ratio of tumor to normal nuclei was verified against pathologist-derived assessment ...
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bioRxiv - Neuroscience 2021Quote: ... or 3.3 mM (Figure 5-figure supplement 1 and 8) ATP and 5 µM ProfilerPro Kinase Peptide Substrate 11 5-FAM-KKLNRTLSVA-COOH (PerkinElmer, U.S.A.), in the presence or absence of 0.66 µM CaM (Sigma-Aldrich ...
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bioRxiv - Genomics 2020Quote: ... washing (3 times 5 min in TNT) and 8 min incubation using Fluorescein Tyramide (for pimonidazole NEL701A001KT, perkin Elmer) or Cy3 (NEL704A001KT, Perkin Elmer) 1:50 in amplification diluent.
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bioRxiv - Molecular Biology 2019Quote: HUVEC cells were seeded in 6 cm2 plates (4 x 105 cells/well) and 8 hours after plating were pulsed for 2 hours with tritiated uridine (Uridine [5,6-3H]; Perkin Elmer; NET367001MC) at final concentration of 2 µCi/ml ...
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bioRxiv - Biochemistry 2021Quote: ... The proteins were separated on 7% tris-glycine or 8% tricine gels by SDS-PAGE and transferred to a PVDF membrane (Perkin Elmer). The proteins were revealed using a V5-monoclonal antibody (V5-mAb V2660 ...
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bioRxiv - Immunology 2023Quote: ... B cells were lysed in water by osmotic pressure and were transferred from 96 well plates to glass fiber filtermat (8×12 Filtermat A, GF/C, 100/pk, #1450-421, Perkin Elmer). The filters were rinsed with water and sealed in plastic bags with a scintillation cocktail (OptiScint® LLT ...
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T-cell development and activation in humanized mice lacking mouse major histocompatibility complexesbioRxiv - Immunology 2024Quote: ... mice were analyzed at week 8 and 12 post-HCT by BLI analyses using the IVIS SpectrumCT (PerkinElmer, Waltham, MA, USA) as described (Theobald et al ...
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bioRxiv - Cell Biology 2020Quote: Fully differentiated C2C12 MS2-CAS myotubes were starved in serum-and glucose-free DMEM for 2 hrs before a 3-hr incubation in low-glucose DMEM/fatty acid-free BSA 0.2% supplemented with radiolabeled glucose (Glucose, D-[3-3H]; 1 μCi/ml; PerkinElmer). Then ...
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bioRxiv - Microbiology 2021Quote: ... The reaction was then stopped with the addition of 0.16 N sulfuric acid and the absorbance measured at 450 nM on an EnSight multimode plate reader (PerkinElmer). Data were in-column normalized using cells containing serum only or no serum with buffer addition were used as 100% and 0% signal ...
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bioRxiv - Biochemistry 2021Quote: ... bound to albumin (1:1 molar ratio) or 0.1 μM [1-14C]oleic acid (2 μCi/dish Perkin Elmer) bound to albumin (1:1 molar ratio ...
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bioRxiv - Biochemistry 2021Quote: ... The radioactivity of the acid-insoluble pellet was measured through the addition of 50μL of MicroScint PS (Perkin Elmer), vortexing ...
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bioRxiv - Cell Biology 2021Quote: ... [9,10(n)-3H]oleic acid (OA) (54.6 Ci/mmol) and [14C]glycerol (250 μCi/mmol) were from Perkin Elmer. Inhibitors of DGAT1 (PF-04620110) ...
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bioRxiv - Biochemistry 2023Quote: ... 2-[1,2-3H(N)]-deoxy-D-glucose and [1,2-14C]-acetic acid were purchased from PerkinElmer (Waltham, MA, USA). PPARγ ...
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bioRxiv - Bioengineering 2023Quote: ... with primers from IDT (Leuven, Belgium) and analyzed on a LabChip® GX Touch Nucleic Acid Analyzer (Perkin Elmer). Plasmids with correct fragment size were amplified by PCR using Q5® High-Fidelity DNA polymerase (NEB ...
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bioRxiv - Microbiology 2021Quote: ... 1-3ng/μl of the PCR amplifier of the HPV L1 gene was mixed with 8 μL of ABI Prism BigDye Terminator Cycle Sequencing Ready Reaction kit version 1.1(Perkin Elmer Biosystems, USA), 2 pmol of primer ...
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bioRxiv - Plant Biology 2019Quote: ... Absorption was measured at 213.8 nm for Zn and 248.3 nm for Fe with an atomic absorption spectrometer (model 11003, manufactured by PerkinElmer, Waltham, MA, USA). A blank was subtracted from the measured values and the mineral concentration of every sample was calculated by using fitting standard solutions.
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bioRxiv - Microbiology 2019Quote: ... and 500 µl were transferred to 2 ml Eppendorf tubes and spiked with 2.5 µCi of 32P-orthophosphoric acid (Perkin Elmer). The cultures were grown for two generations (OD600 0.2 ...
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bioRxiv - Biochemistry 2021Quote: Structural models of polyenoyl tetramic acids 3 and 4 were built and energy minimized using Chem3D v.16.0 (Perkin Elmer). The terminal region of polyenes ...
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bioRxiv - Cancer Biology 2020Quote: ... The cells were then washed twice with PBS and incubated with either H-H or L-L medium supplemented with 1μCi per well of L-[U-14C]-Aspartic acid (PerkinElmer, NEC268E050UC) for 40 h ...
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bioRxiv - Physiology 2020Quote: ... The integrity of resultant cDNA was assessed using a LabChip GX Touch 24 Nucleic Acid Analyzer as per manufacturer’s instructions (Perkin Elmer).
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bioRxiv - Immunology 2022Quote: ... Quality and quantity were checked using a Caliper LabChip GX Touch HT Nucleic Acid Analyzer (PerkinElmer, Waltham, MA 02452 USA). The PCR product library was quantitated and subjected to sequencing on an Illumina MiSeq sequencer (Illumina ...
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bioRxiv - Immunology 2022Quote: ... using the RNA Assay and DNA 5K/RNA/CZE HT Chip on a LabChip GX Touch HT Nucleic Acid Analyzer (PerkinElmer). TruSeq Stranded mRNA Library Prep kit (Illumina ...
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bioRxiv - Microbiology 2022Quote: ... DNA quantification and quality check was done using a LabChip GX Touch HT Nucleic Acid Analyzer (PerkinElmer, Waltham, MA, USA). Libraries were prepared as detailed on the Illumina® support website (http://support.illumina.com ...
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bioRxiv - Biochemistry 2023Quote: ... Protein biosynthesis rates were determined by measuring the incorporation of radioactive methionine in auranofin-treated and untreated samples after protein precipitation with 20 % (w/v) trichloro acetic acid using a scintillation counter (Tri-Carb 2800TR, PerkinElmer). 55 µg of protein from pulse-labeled samples for analytical gels and 300 µg of protein from unlabeled samples for preparative gels were rehydrated into IPG strips (24 cm ...
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bioRxiv - Neuroscience 2023Quote: ... The saturation binding assay was conducted using an 8-point dose-dependent concentration of [3H] ketanserin (maximal concentration 20 nM) (Perkin Elmer, Waltham, MA) with brain crude membranes (25 μg membrane proteins ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were then treated for 10 minutes with indicated compounds before addition of 20 μCi of 32P-ortho-phosphoric acid (Perkin Elmer). Cells were incubated for 1 hour with media containing drugs and 32P-ortho-phosphoric acid ...