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Citations for PerkinElmer :
101 - 150 of 500 citations for 7 Hydroxy 4 methylcoumarin 3 acetic acid succinimidyl ester since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: GBM2 cells were plated at 2000 cells/well and exposed to Prestwick compounds (3 µM; Table 6) for 3 days in 384-well optical bottom assay plates (PerkinElmer). Cells were then fixed and stained with rabbit polyclonal anti-H3K27ac and mouse monoclonal anti-H3K27me3 antibodies followed by AlexaFluor-488- or AlexaFluor-555-conjugated secondary antibodies ...
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bioRxiv - Developmental Biology 2021Quote: ... 3-D reconstruction was performed with Volocity (PerkinElmer).
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bioRxiv - Cell Biology 2021Quote: ... TSA® Plus Cyanine 3 (PerkinElmer, 1:1,500) was used as a secondary fluorophore for Hbs1l-C2 probes.
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bioRxiv - Microbiology 2021Quote: ... which contained trace amount of 32P-labeled orthophosphoric acid (∼1 µCi, Perkin Elmer) and different concentrations of cold PO4 (from 0.02 μM to 20 μM) ...
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bioRxiv - Microbiology 2022Quote: ... Nucleic acids were collected on filters with a cell harvester (PerkinElmer Life Sciences). After addition of MicroScint O (PerkinElmer Life Sciences) ...
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bioRxiv - Cancer Biology 2021Quote: ... Multispectral immunohistochemistry was performed using the Opal 7-Color Manual IHC Kit (Akoya Biosciences/PerkinElmer, NEL811001KT, Marlborough, MA) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... The medium was removed and 125I-GLP-1(7-36)NH2 (60 pM) or 125I-GCG (40 pM) (PerkinElmer) and increasing concentrations unlabeled tirzepatide or peptide 20 were added for overnight incubation at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... A fragmentation step was carried out for 6.5 minutes at 94°C and 7 μM NextFlex DNA barcodes (PerkinElmer) were used for adapter ligation ...
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bioRxiv - Cell Biology 2023Quote: ... by incubating 200 ng of DNA with 7 μL of TNT-T7 reticulocyte lysate mix and 0.5 µL of 35S-labelled methionine (NEG709A500UC, PerkinElmer) for 1 h at 30 °C at 600 rpm ...
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bioRxiv - Biochemistry 2023Quote: ... The samples were then separated on a HayeSep column (7’ HayeSep N 1/8’’ Sf; PerkinElmer Inc., Waltham, USA), followed by molecular sieve (9’ Molecular Sieve 13x 1/8’’ Sf ...
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bioRxiv - Neuroscience 2024Quote: ... were collected from the CA1 SR from ≥5-7 sections per slice and processed for iterative deconvolution (99% confidence; Volocity 4.0, PerkinElmer). Three dimensional (3-D ...
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bioRxiv - Biochemistry 2021Quote: ... The lysate was mixed with Optiphase Hisafe 3 (PerkinElmer), and radioisotope activity was monitored by LSC-8000 β-scintillation (Hitachi) ...
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bioRxiv - Neuroscience 2020Quote: ... then incubated in TSA plus cyanine 3 (Perkin Elmer) for 10 min ...
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bioRxiv - Cancer Biology 2019Quote: ... A Vectra 3 pathology imaging system microscope (PerkinElmer, Inc.) was used to obtain images ...
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bioRxiv - Neuroscience 2020Quote: ... The luminescent intensity was measured with VICTOR 3 (PerkinElmer), and the values were normalized to the MAO B protein level measured by Western blotting.
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bioRxiv - Physiology 2020Quote: ACSL initial rates were measured with 50 μM [1-14C]palmitic acid (Perkin Elmer), 10 mM ATP ...
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bioRxiv - Microbiology 2021Quote: ... cultures were grown with 2.5 μCi/mL of 32P orthophosphoric acid (32P) (Perkin Elmer) and lipid A was extracted ...
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bioRxiv - Developmental Biology 2022Quote: ... The composition of fatty acids was analyzed by gas chromatography (Perkin Elmer Clarus 680) employing a flame ionization detector with an SP2300 fused silica capillary column (30 m × 0.25 mm × 0.2 μm ...
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bioRxiv - Bioengineering 2022Quote: ... Volatile fatty acid (VFA) analysis was done in gas chromatography (PerkinElmer LLC, MA, USA) equipped with flame ionization detector (FID ...
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bioRxiv - Genetics 2024Quote: ... Total nucleic acid was extracted from the tissues using the Chemagic 360 instrument (PerkinElmer) and quantified with Qubit DNA High Sensitivity Kit (Catalogue Number ...
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bioRxiv - Pathology 2021Quote: ... primary hepatocytes were serum-starved for 3 hours and incubated for 3-hour with [1-14C] acetate (1 μCi/ml; Perkin Elmer, Boston, MA) and 5.5 mM of non-labeled (cold ...
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bioRxiv - Cancer Biology 2021Quote: ... Bioluminescent imaging was performed every 3-7 days following intraperitoneal injection of D-luciferin and measured using the Xenogen IVIS Spectrum in vivo imaging system (PerkinElmer). Living Image software (PerkinElmer ...
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bioRxiv - Immunology 2022Quote: ... 0.2 µM kinase was incubated with 7 µM substrate and 0.2 mM ATP containing 0.45 mCi [32P]-γ-ATP/mL (Perkin Elmer) in kinase buffer (50 mM HEPES pH 7.6 ...
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bioRxiv - Immunology 2023Quote: ... The bioluminescent signal emitted by the disseminated tumors was measured at 7-day intervals for 2 months using the IVIS Spectrum In Vivo Imaging System (PerkinElmer). The weight of the mice was recorded before each measurement.
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bioRxiv - Molecular Biology 2023Quote: ... Each condition was imaged from duplicated wells and a minimum of 7 fields/well using Operetta high-content imaging system 1483 (PerkinElmer). The measurement of p16 and p21 fluorescence intensity per area (normalised to the number of cells ...
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bioRxiv - Neuroscience 2021Quote: ... after 1 hour at 37°C the reaction was stopped with 0.25 M glycine buffer pH 10.4 and the fluorescence emission of the 4-methylumbelliferyl generated (4-MU) by the reaction was read with a fluorimeter (EnSpire Plate Reader, PerkinElmer) selecting an excitation wavelength of 365 nm and an emission wavelength of 445 nm ...
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bioRxiv - Biochemistry 2023Quote: ... in the presence of 20 µM of the corresponding L-[14C]-amino acid (Perkin Elmer). AaRSs were diluted in 100 mM HEPES-KOH pH 7.5 ...
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bioRxiv - Cancer Biology 2019Quote: ... the Opal 4-colour IHC kit (Perkin Elmer) was used per manufacturer’s protocol.
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bioRxiv - Molecular Biology 2022Quote: ... 4 µl of [γ-32P] ATP (PerkinElmer, #BLU502A001MC), and 10 units of T4 polynucleotide kinase (PNK ...
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bioRxiv - Biochemistry 2022Quote: ... 4 μL of the acceptor beads (Perkin Elmer) diluted in PBS was added to each well in the dark and incubated for 90 mins ...
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bioRxiv - Biochemistry 2022Quote: ... 4 μL of the donor beads (Perkin Elmer) diluted in PBS was added to each well to give a total volume of 25 μL and incubated for 2 hours ...
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bioRxiv - Neuroscience 2020Quote: ... and a Wallac 1420 VICTOR 3 V luminometer (Perkin Elmer) according to the manufacturer’s protocol.
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bioRxiv - Neuroscience 2020Quote: ... TSA® Plus Cyanine 3 (1:1000, Perkin Elmer NEL744E001KT), and TSA® Plus Cyanine 5 (1:1000 ...
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bioRxiv - Developmental Biology 2020Quote: ... and DLX5 were carried out using Cyanine 3 (PerkinElmer NEL752001KT) diluted 1:500 in RNAscope Multiplex TSA Buffer ...
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bioRxiv - Cell Biology 2020Quote: ... A TSA Cyanine 3 Signal Amplification System (#SAT704A001EA, Perkin Elmer) was used for signal detection according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... and TSA Plus Cyanine 3 System (Perkin Elmer, 1:50). Nuclear staining was obtained using DAPI (Sigma-Aldrich ...
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bioRxiv - Immunology 2020Quote: ... TSA Cyanine 3 or Cyanine 5 amplification kits (Perkin Elmer) were used according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... Cyanine-3 (Cy3 Direct FISH; PerkinElmer Life Sciences, Waltham, MA) was used to visualize labeled cells and nuclei were counterstained with DAPI (ThermoFisher Scientific).
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bioRxiv - Microbiology 2023Quote: ... The filters were soaked in 3 mL ULTIMA Gold (PerkinElmer) overnight before liquid scintillation counting ...
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bioRxiv - Neuroscience 2022Quote: ... TSA fluorophores for cyanine 3 and cyanine 5 (Perkin Elmer) were used at a concentration of 1:1000 ...
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bioRxiv - Cell Biology 2023Quote: ... and AKT 1/2/3 (p-S473) Assay Kits (PerkinElmer). Cell lysis and SureFire Assay were performed following the manufacturer’s instructions.
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bioRxiv - Neuroscience 2023Quote: ... before gamma counting using a gamma counter (Wizard 3, PerkinElmer). [18F]-FDG uptake was normalized by quantity of protein for each well.
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bioRxiv - Neuroscience 2023Quote: ... or TSA Plus Cyanine 3 (Cy3; 1:100; PerkinElmer; #NEL744001KT). Sections were imaged on an Olympus AX70 microscope with a Zeiss AxioCam HRcRv.2 camera ...
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bioRxiv - Microbiology 2019Quote: ... After enzymatic conversion the fluorescent signals corresponding to the amount of released 4-methylumbelliferone (4-MU) were quantified in a microplate reader (Victor X2, Perkin Elmer) with the excitation and emission wavelengths of 355 and 460 nm ...
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bioRxiv - Biochemistry 2021Quote: ... Thirty μL of tissue homogenate supernatant were mixed with 370 μL of the oxidation reaction mixture containing 7% BSA/5 mM palmitate/0.01 μCi/μL 14C-palmitate (PerkinElmer, Waltham, MA) and incubated at 37 °C for 30 min ...
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bioRxiv - Developmental Biology 2021Quote: ... Successfully mounted embryos were then imaged starting between 2 and 7 ss using a 20X objective on a Perkin-Elmer UltraVIEW Vox spinning disk confocal microscope (Perkin Elmer) while maintained at 32°C ...
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bioRxiv - Biochemistry 2021Quote: ... The proteins were separated on 7% tris-glycine or 8% tricine gels by SDS-PAGE and transferred to a PVDF membrane (Perkin Elmer). The proteins were revealed using a V5-monoclonal antibody (V5-mAb V2660 ...
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bioRxiv - Neuroscience 2022Quote: ... was diluted 1:500 in carbonate buffer pH 9.7 (25 mM sodium bicarbonate, 25 Mm sodium carbonate) and coated in flat-bottom white plates (OptiPlate 96F-HB, Perkin Elmer) overnight at 4°C under agitation ...
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bioRxiv - Microbiology 2022Quote: ... The production of LepB–SecM and LepB–CutF variants was induced by the simultaneous addition of 0.1 M IPTG and 2 µl of L-[35S] methionine-cysteine (7 mCi/ ml, PerkinElmer Life Sciences) (38) ...
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bioRxiv - Neuroscience 2023Quote: ... in the presence of 7 increasing concentrations (0.15 – 10 nM) of [3H]-raclopride (specific activity 82.8Ci/mmol, Perkin Elmer, #NET975250UC, Canada) for D2 binding ...