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Citations for PerkinElmer :
351 - 400 of 505 citations for 7 9 Diazaspiro 4.5 decane 6 8 10 trione 7 ethyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... 100 nM Ocr was mixed with 128 µM ATP supplemented with 10 nM [α- 32 P]-ATP (PerkinElmer) in the reaction buffer ...
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A distinctive ligand recognition mechanism by the human vasoactive intestinal polypeptide receptor 2bioRxiv - Molecular Biology 2021Quote: CHO-K1 cells were cultured in F12 medium with 10% FBS and seeded at a density of 30,000 cells/well in Isoplate-96 plates (PerkinElmer). Twenty-four hours after transfection with the WT or mutant receptors ...
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bioRxiv - Biochemistry 2019Quote: ... Oligonucleotides were 5’-end-labeled with [γ-32P]-ATP (10 mCi ml-1, 3000 Ci mmol-1; Perkin Elmer) using polynucleotide kinase (Thermo Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... Plates were incubated for 10 mins at room temperature and then were analyzed on the Envision plate reader (PerkinElmer). Relative light units (RLUs ...
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bioRxiv - Microbiology 2021Quote: ... incubated for 10 min at RT and luciferase signal was quantified on a VICTOR Nivo plate reader (Perkin Elmer).
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bioRxiv - Cancer Biology 2020Quote: ... the slides were then incubated at RT for 10 min with one of the following Alexa Fluorescent tyramides (PerkinElmer) included in the Opal 4 colour kit (NEL810001KT ...
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bioRxiv - Microbiology 2021Quote: ... Plates were incubated for 10 min at room temperature and then were analyzed on the Envision plate reader (PerkinElmer). Relative light units (RLUs ...
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bioRxiv - Cancer Biology 2020Quote: ... the slides were then incubated at RT for 10 min with one of the following Alexa Fluorescent tyramides (PerkinElmer) included in the Opal 4 color kit (Cat No ...
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bioRxiv - Molecular Biology 2021Quote: CHO-K1 cells were cultured in F12 medium with 10% FBS and seeded at a density of 30,000 cells/well in Isoplate-96 plates (PerkinElmer). The WT (HA-Flag-3GSA-GIPR(22-466) ...
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bioRxiv - Molecular Biology 2021Quote: ... CHO-K1 cells were cultured in F-12 medium with 10% FBS and seeded at a density of 30,000 cells/well in Isoplate-96 plates (PerkinElmer). The wild-type (WT ...
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bioRxiv - Microbiology 2022Quote: ... oligonucleotides were 5’-end-labelled with [γ-32P]-ATP (10 mCi ml-1, 3000 Ci mmol-1, Perkin Elmer) with polynucleotide kinase (Thermo Scientific) ...
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bioRxiv - Microbiology 2022Quote: ... 10 μL of lipid extracts were mixed with 2 mL of Ultima Gold™ scintillation fluid (Perkin Elmer, USA), and radioactive counts were measured using a MicroBeta2® scintillation counter (Perkin Elmer ...
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bioRxiv - Microbiology 2022Quote: ... 10 μCi of either [1,2-3H(N)] free cholesterol or [1,2-3H(N)]cholesteryl oleate (Perkin Elmer, Boston, MA) were mixed with absolute ethanol ...
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bioRxiv - Microbiology 2022Quote: ... Reactions contained 10 μL rabbit reticulocyte lysate with 500 ng of pcDNA T7 expression plasmid and 0.5 μL [35S] methionine (PerkinElmer). Reactions were incubated at 30°C for 40 minutes before being chased with 2 μl of 50 mg / mL unlabelled methionine ...
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bioRxiv - Microbiology 2023Quote: ... Reactions contained 10 μL lysate with 250 ng of pcDNA T7 expression plasmid and 0.5 μL [35S] methionine/cysteine (PerkinElmer). Reactions were incubated at 30°C for 40 minutes chasing with 2 μL of 50 mg / mL unlabelled methionine/cysteine ...
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bioRxiv - Biochemistry 2023Quote: ... The mice were anesthetized in a ventilated anesthesia chamber with 1.5% isofluorane in oxygen and imaged 10 min after the injection with an in vivo imaging system (IVIS, PerkinElmer). Luminescence was quantified using the Living Image software (PerkinElmer) ...
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bioRxiv - Cell Biology 2022Quote: Candidate small RNA sequences were identified from raw sequence data using cutadapt (ver. 2.8)21 with parameters “-u -4 -a A(10)” as recommended by PerkinElmer NextFlex Combo-Seq kit ...
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bioRxiv - Cell Biology 2023Quote: ... quantitative microscopy was performed using the Operetta HCS imaging system with a 10×/0.3 NA objective (PerkinElmer, Courtaboeuf, France). Images were analyzed using scripts developed in Acapella software (PerkinElmer ...
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bioRxiv - Biochemistry 2023Quote: ... 10 μM purified IKK2 protein was first autophosphorylated in the presence of 100-200 nM of γ32P-ATP (PerkinElmer) in kinase assay buffer for 1 hour at 30°C ...
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bioRxiv - Cell Biology 2024Quote: ... increasing concentrations of carbachol (from 0.01 µM up to 10 µM) were used in presence of 200 pM [3H]N-Methylscopolamine ([3H]NMS; 78.9 Ci/mmol; PerkinElmer).
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bioRxiv - Evolutionary Biology 2020Quote: Bioluminescence was measured in 100 μL culture (or a 10-fold dilution series) in an Envision Multilabel Plate Reader (PerkinElmer). Clonal bioluminescence was also qualitatively assessed by examining colonies on LB agar in an AlphaImager HP light box (ProteinSimple ...
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bioRxiv - Immunology 2021Quote: ... The luciferase signal was determined in live animals one day after virus injection and 10 minutes after intraperitoneal injection of 150 mg/kg D-luciferin (PerkinElmer) using the in vivo imaging system NightOWL II LB 983 (Berthold ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The plates were read every 10 minutes on a fluorescence microplate reader (EnVision 2104 Multilabel Reader (Perkin Elmer, MA, USA) at an excitation wavelength of 440 nm with an emission wavelength of 484 nm ...
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bioRxiv - Cancer Biology 2019Quote: ... 150 mg/kg luciferin was intraperitoneally injected into mice 10 minutes prior to imaging with IVIS Spectrum Imaging Systems (Perkinelmer). Imaging settings remained the same throughout the study and luminescence intensity was measured using Living Image Software (Perkinelmer).
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bioRxiv - Cancer Biology 2019Quote: ... Luminescence imaging carried out every 2-3 days using an IVIS luminescence imaging system 10 min after mice were injected with 10 μl/g of body weight 15 mg/mL in vivo grade D-Luciferin (PerkinElmer). Mice were sacrificed by CO2 asphyxiation followed by cervical dislocation if they reached AAALAC-defined endpoints ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The plates were read every 10 minutes on a fluorescence microplate reader (EnVision 2104 Multilabel Reader (Perkin Elmer, MA, USA) at an excitation wavelength of 440nm with an emission wavelength of 484nm.
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bioRxiv - Pharmacology and Toxicology 2020Quote: Spectra were analysed using OPUS 6.5 software (Bruker) and compared with RMIT University’s spectral library of organic compounds generated using Spectrum 10 software (Perkin Elmer). Fingerprint spectra shown in Figure 4D were prepared using GraphPad Prism.
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bioRxiv - Biochemistry 2021Quote: ... The samples were boiled at 95°C for 10 min and subjected directly to LabChip GX II capillary gel electrophoresis (PerkinElmer) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2019Quote: ... membranes were incubated with increasing free concentrations of [3H] SCH 23390 (0.02 nM to 10 nM, PerkinElmer, Boston, MO, USA) or [3H]R-a-methyl histamine (0.1 nM to 20 nM [3H]RAMH ...
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bioRxiv - Cell Biology 2021Quote: ... 10mM MgCl2 and 1 mM MnCl2) supplemented with 10 μM ATP and 2 μCi γ-32P-ATP (blu002z250uc; Perkin Elmer) in 15μl reactions ...
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bioRxiv - Cancer Biology 2022Quote: ... luciferin was added to each well (10% of total volume per well) and plates were imaged using the IVIS Spectrum In Vivo Imaging System (PerkinElmer). Cell viability was measured as total photon flux per second ...
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bioRxiv - Neuroscience 2021Quote: ... during 2 hours at RT followed by a 10 minutes incubation at RT with TSA plus Biotin System (Biotin TSA 1:250, NEL749A001KT PerkinElmer). After several washes ...
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bioRxiv - Neuroscience 2021Quote: ... during 2 hours at RT followed by a 10 minutes incubation at RT with TSA Biotin System (Biotin TSA 1:100, NEL700A001KT PerkinElmer). After several washes ...
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bioRxiv - Neuroscience 2021Quote: ... The signal of CB1 receptor hybridization was revealed by a TSA reaction using cyanine 3 (Cy3)-labeled tyramide (1:100 for 10 minutes, NEL744001KT PerkinElmer). After several washes ...
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bioRxiv - Biochemistry 2019Quote: ... transcription involved the T-less template and inclusion of 10 μCi α-32P-UTP (3000 Ci/mmol) UTP (Perkin Elmer).
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bioRxiv - Cell Biology 2019Quote: GBM2 cells were plated at 4000 cells/well and exposed to epigenetic compounds (Table 2) at 10 µM for 1 day in 384-well optical bottom assay plates (PerkinElmer). Negative control was DMSO (0.1%) ...
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bioRxiv - Molecular Biology 2021Quote: ... from which aliquots of 10 μL were counted in vials with 3 mL OptiPhase HiSafe3 scintillation fluid (PerkinElmer, #1200-437). The fraction of charged tRNAAsn was calculated based on the charged tritium labeled aspartate incorporated into the pellet ...
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bioRxiv - Biochemistry 2021Quote: ... 25 mM GTP and 0.25 mM CTP) and 0.5 µl of α-32P-CTP (3000 Ci/mmol, 10 mCi/ml Perkin Elmer) were supplemented into each reaction along with 1 ng of DNA template and 100 ng of competitor DNA ...
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bioRxiv - Physiology 2020Quote: ... sections were incubated with an HRP-conjugated secondary antibody at an optimised dilution in NGS for 30 min at room temperature followed by a 10 min incubation with Tyramide solution (PerkinElmer). Primary and secondary antibodies and associated working dilutions are given in Supplementary Table 1 ...
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bioRxiv - Genomics 2021Quote: ... and transferred to a plastic scintillation vial containing 10 mL of OptPhase HiSafe 3 scintillation cocktail (PerkinElmer, Waltman, MA, USA). All vials were counted for 10min using a Tri-Carb 2810 TR liquid scintillation analyzer (PerkinElmer ...
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bioRxiv - Cancer Biology 2022Quote: ... 15 or 10-20% SDS-PAGE gels and proteins were transferred onto PVDF membranes (0.45 μm, Perkin Elmer, Waltham, MA) and blocked with 5% BSA/TBS-T for 2 hours at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... and imaged 10 minutes after D-luciferin injection with a cooled charge-coupled device (CCD) camera (In Vivo Imaging System by PerkinElmer). Mice were imaged regularly every week until week 8 and every 4 weeks until week 28 ...
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bioRxiv - Genetics 2023Quote: ... 10 µg of total RNA was polyA-selected and processed using the NEXTFLEX Rapid Direction RNA-seq Kit (NOVA-5138-10 and NOVA-5138-11; PerkinElmer) according to the provided manual ...
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bioRxiv - Cancer Biology 2023Quote: ... mice were given 10 μL/g of D-luciferin (15 mg/mL) (Gold Bio) intraperitoneally and imaged using the IVIS Spectrum imager (PerkinElmer). Luciferase signal was quantified 15 min after injection ...
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bioRxiv - Systems Biology 2023Quote: ... A breathable membrane was applied over the plate and cells were imaged every 10 min on the Operetta CLS high-content microscope (PerkinElmer) at 37°C and 5% CO2 for 72h using the 20x N.A ...
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bioRxiv - Biochemistry 2023Quote: ... was mixed in 20 µl of the cytoplasmic buffer with 2- to 10-fold excess of recombinant Sm proteins and each sample was supplemented with 2 µCi of 3H-labeled SAM (PerkinElmer) for overnight methylation at 32 °C ...
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bioRxiv - Microbiology 2023Quote: ... Plates were read once every 30 s for 10 min at 37°C using a EnSpire Multimode Plate Reader (PerkinElmer) with excitation and emission wavelengths of 550 and 591 nm ...
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bioRxiv - Microbiology 2023Quote: ... purified 6×His-tagged SfTIR-STING was added to the reaction to a final assay volume of 50 μL and plates were read once every 15 s for 10 min at 37°C using a EnSpire Multimode Plate Reader (PerkinElmer) with excitation and emission wavelengths of 410 and 300 nm ...
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bioRxiv - Cancer Biology 2023Quote: ... and tissues were rapidly imaged (within 10 minutes post-sacrifice) with the bioluminescence signal being assessed with the IVIS Spectrum In Vivo Imaging System (PerkinElmer). Tissue tumor burden was calculated based on the total flux (photons per second [p/s] ...
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bioRxiv - Molecular Biology 2023Quote: ... was done using the same procedure for the cold transcription except for the use of 0.5 µl alpha-32P-labeled UTP (3000 Ci/mmol-10 mCi/ml) (Perkin Elmer) and 0.8 mM UTP (instead of 2 mM) ...