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Citations for PerkinElmer :
551 - 600 of 736 citations for 6 Chloro 9 fluorobenz 9 isoquinoline 5 10 dione since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Hybridization signals were detected by fluorescent signal using peroxidase-based Tyramide Signal Amplification-Plus Fluorescein or TSA Plus Cyanine 5 (Perkinelmer, Shelton, CT). Finally ...
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bioRxiv - Biophysics 2023Quote: ... stand and a 40 nt oligo-dT at the 5′ end was labeled at the 3′ terminus with [α-32P] dCTP (Perkin Elmer) and annealed to the single-stranded DNA M13mp18(24) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Determination of Gαi and Gαs selective signaling at the 5-HT1AR was performed applying an IP accumulation assay (IP-One HTRF®, PerkinElmer, Rodgau, Germany) according to the manufacturer’s protocol and in analogy to previously described protocols (15 ...
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bioRxiv - Systems Biology 2023Quote: ... The NPCs were cultured in a 12-well dish to confluency in NPC media and then dissociated using Accutase for 5 minutes at 37°C to single cell resolution and plated on 5 µg/mL human laminin and 5µg/mL fibronectin-coated 384 well plates (Perkin Elmer Cat #6057308) at 12,000 cells/well in NPC media and 10 µM ROCKi ...
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bioRxiv - Immunology 2023Quote: ... Fluorescent signals were monitored in real-time with a microplate reader for 24 h within a tissue culture incubator (37 °C, 5 % CO2) on black OptiPlate 96-well (PerkinElmer, MA, USA) under 20x objective ...
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bioRxiv - Developmental Biology 2024Quote: ... Plates (with lid on) were placed in the Opera Phenix High-Content Screening System (Perkin Elmer; prewarmed to 37C and 5% CO2). Images were collected every 30 min for 24 hours.
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bioRxiv - Molecular Biology 2020Quote: ... 10 images of each section were randomly collected and analyzed by the Columbus v.2.4.2 software (Perkin Elmer) with an algorithm adapted for Oil Red-O stained sections ...
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bioRxiv - Cell Biology 2021Quote: ... 3 10-minute TBST washes were performed followed by Western Lightning plus ECL Enhanced Chemiluminescence (Perkin Elmer, MA). Proteins were detected on X-ray film (Fuji ...
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bioRxiv - Cell Biology 2022Quote: ... 3 mL of the hexane layer was added to 10 mL UltimaGold liquid scintillation cocktail (PerkinElmer, Waltham, USA) and measured on a TriCarb 2800TR liquid scintillation counter (PerkinElmer ...
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bioRxiv - Zoology 2019Quote: ... Three tubes (final volume of 150 µL) were prepared for each sample: two containing 160 nM cortisol (10% 1,2,6,7-3H-cortisol, Perkin Elmer, Waltham ...
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bioRxiv - Biochemistry 2020Quote: ... pH 7.4) with 10 μM 2-deoxy-Glucose and 0.5 μCi/mL 3H-2-deoxy-glucose (Perkin Elmer). Cytocholasin B (5 μM ...
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bioRxiv - Evolutionary Biology 2020Quote: ... tritiated [10-3H(N)]-JH III (specific activity 19.4 Ci/nmol, Perkin Elmer Life Sciences, Waltham, MA, USA), and synthetic juvenile hormone-III (Fluka ...
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bioRxiv - Cancer Biology 2021Quote: ... Plates were incubated for 10 minutes at room temperature and luminescence determined using an EnVision Multilabel reader (PerkinElmer) and the readings taken as the measure of cell growth ...
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bioRxiv - Cancer Biology 2021Quote: ... was added and incubated for 10 min before luminescence was measured with a multimode microplate reader (PerkinElmer, EnVision). Data were fitted into an inhibitor-response model to get IC50 values.
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bioRxiv - Cancer Biology 2022Quote: ... supplemented with 10% Fetal Bovine Serum (100 uL) in 384-well CellCarrier Ultra plates (PerkinElmer, Waltham, Massachusetts, USA). For time-lapse microscopy ...
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bioRxiv - Biochemistry 2022Quote: ... 100 μM cold ATP and 1 μCi [γ-35P] ATP (6000 Ci/mmol,10 mCi/ml, Perkin Elmer) with recombinant proteins (200-500 ng ...
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bioRxiv - Molecular Biology 2022Quote: ... 10 μL immunoprecipitated enzyme were mixed with 300 μM synthetic peptide and 50 μM [14C]-acetyl-CoA (PerkinElmer) in acetylation buffer (50 mM HEPES pH 8.5 ...
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bioRxiv - Biochemistry 2023Quote: ... and 10 mM DTT) were combined in a 384 well plate (PerkinElmer ProxiPlate-384 Plus F black, #6008260) and data gathered on a Envision 2150 plate reader (PerkinElmer ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and biotinylated-JQ1 at 20nM was added in 10 µL to 384-well plates (AlphaPlate-384, PerkinElmer, USA). Plates were spun down at 150x g ...
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bioRxiv - Cancer Biology 2023Quote: ... and imaged live for 10 hours using the Operetta High Content Screening System (Perkin Elmer, see section 3.2.6) at 37 °C and 5% CO2 at intervals of 20 mins ...
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bioRxiv - Molecular Biology 2024Quote: ... anti-FLAG donor and protein G coated acceptor beads (10 ng/μl final concentration; AS103D and AL102C, PerkinElmer). For the competitive assay with CD81-GFP and antiHER2 EVs ...
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bioRxiv - Biochemistry 2024Quote: ... 100 nM Ocr was mixed with 128 µM ATP supplemented with 10 nM [α- 32 P]-ATP (PerkinElmer) in the reaction buffer ...
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bioRxiv - Evolutionary Biology 2021Quote: ... biotin-labelled probes were visualized by streptavidin-horseradish peroxidase (HRP) conjugates with Cyanine 5 Plus Amplification Reagent in a TSA kit as the substrate (PerkinElmer, Boston, MA, USA). Sense probes labelled with DIG and biotin by SP6 RNA transcription kit were used as control ...
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bioRxiv - Genetics 2019Quote: ... with the exception that the blue and green probes were labeled with coumarin-5-dUTP (custom synthesis, Perkin Elmer Life and Analytical Sciences) and fluorescein-12-dUTP (Perkin Elmer) ...
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bioRxiv - Microbiology 2021Quote: ... The washed filters were dried for 30 min under a heat-lamp and placed in a 20-ml Borosilicate Glass Scintillation vial containing 5 ml of Ultima Gold F liquid scintillation cocktail (Perkin Elmer, MA, USA). 14C radioactivity retained on the filters was quantified using an LS6500 scintillation system (Beckman Coulter Inc. ...
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bioRxiv - Bioengineering 2022Quote: Cu and Zn ion release from PCu coupons (surface area 5 cm2) and Cu-coated SS coupons was measured using inductively coupled plasma optical emission spectrometry (ICP-OES, Perkin Elmer, Optima 8300). The PCu coupons were wet ground with a 600 grit SiC emery paper and rinsed with DI water ...
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bioRxiv - Neuroscience 2022Quote: ... or 1 μg of GST-Raf13 as the Pak1 substrate with a total volume of 5 μL in a 384-well plate (PerkinElmer, Waltham, MA, USA) at 30°C for 1 h ...
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bioRxiv - Cancer Biology 2019Quote: pWPXLD-3xflag-shank2 (5 μg) was incubated with GST-PRMT7 in the presence of S-adenosyl-methionine (SAM; 15 Ci/mmoL; PerkinElmer, Waltham, MA, USA) at 30°C for 1 h ...
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bioRxiv - Molecular Biology 2020Quote: ... The 3’TAMRA fluorescence from 5’UAR was recorded by a single molecule avalanche photodiode (SPAD) (SPCM-AQR-14, PerkinElmer Optoelectronics, Quebec, Canada), through a 600/50 band pass emission filter ...
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bioRxiv - Cell Biology 2022Quote: ... Bioluminescence imaging was performed at days 5 and 7 post engraftment using an IVIS Lumina III in vivo imaging system (Perkin Elmer, Waltham, MA). IVIS instrument exposure time was 3 seconds ...
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bioRxiv - Cell Biology 2023Quote: All the videos (2 min, 4-5 frames s-1) were acquired at 37°C using a Nikon TIRF microscopy system (Perkin Elmer, Waltham, MA) on an inverted Ti microscope equipped with a 100× objective and an ImageEM C9100-13 camera (Hamamatsu Photonics ...
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A distinctive ligand recognition mechanism by the human vasoactive intestinal polypeptide receptor 2bioRxiv - Molecular Biology 2021Quote: CHO-K1 cells were cultured in F12 medium with 10% FBS and seeded at a density of 30,000 cells/well in Isoplate-96 plates (PerkinElmer). Twenty-four hours after transfection with the WT or mutant receptors ...
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bioRxiv - Microbiology 2020Quote: ... Plates were incubated for 10 mins at room temperature and then were analyzed on the Envision plate reader (PerkinElmer). Relative light units (RLUs ...
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bioRxiv - Microbiology 2021Quote: ... incubated for 10 min at RT and luciferase signal was quantified on a VICTOR Nivo plate reader (Perkin Elmer).
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bioRxiv - Cancer Biology 2020Quote: ... the slides were then incubated at RT for 10 min with one of the following Alexa Fluorescent tyramides (PerkinElmer) included in the Opal 4 colour kit (NEL810001KT ...
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bioRxiv - Microbiology 2021Quote: ... Plates were incubated for 10 min at room temperature and then were analyzed on the Envision plate reader (PerkinElmer). Relative light units (RLUs ...
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bioRxiv - Cancer Biology 2020Quote: ... the slides were then incubated at RT for 10 min with one of the following Alexa Fluorescent tyramides (PerkinElmer) included in the Opal 4 color kit (Cat No ...
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bioRxiv - Molecular Biology 2021Quote: CHO-K1 cells were cultured in F12 medium with 10% FBS and seeded at a density of 30,000 cells/well in Isoplate-96 plates (PerkinElmer). The WT (HA-Flag-3GSA-GIPR(22-466) ...
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bioRxiv - Molecular Biology 2021Quote: ... CHO-K1 cells were cultured in F-12 medium with 10% FBS and seeded at a density of 30,000 cells/well in Isoplate-96 plates (PerkinElmer). The wild-type (WT ...
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bioRxiv - Microbiology 2022Quote: ... 10 μL of lipid extracts were mixed with 2 mL of Ultima Gold™ scintillation fluid (Perkin Elmer, USA), and radioactive counts were measured using a MicroBeta2® scintillation counter (Perkin Elmer ...
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bioRxiv - Microbiology 2022Quote: ... 10 μCi of either [1,2-3H(N)] free cholesterol or [1,2-3H(N)]cholesteryl oleate (Perkin Elmer, Boston, MA) were mixed with absolute ethanol ...
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bioRxiv - Microbiology 2022Quote: ... Reactions contained 10 μL rabbit reticulocyte lysate with 500 ng of pcDNA T7 expression plasmid and 0.5 μL [35S] methionine (PerkinElmer). Reactions were incubated at 30°C for 40 minutes before being chased with 2 μl of 50 mg / mL unlabelled methionine ...
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bioRxiv - Microbiology 2023Quote: ... Reactions contained 10 μL lysate with 250 ng of pcDNA T7 expression plasmid and 0.5 μL [35S] methionine/cysteine (PerkinElmer). Reactions were incubated at 30°C for 40 minutes chasing with 2 μL of 50 mg / mL unlabelled methionine/cysteine ...
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bioRxiv - Biochemistry 2023Quote: ... The mice were anesthetized in a ventilated anesthesia chamber with 1.5% isofluorane in oxygen and imaged 10 min after the injection with an in vivo imaging system (IVIS, PerkinElmer). Luminescence was quantified using the Living Image software (PerkinElmer) ...
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bioRxiv - Cell Biology 2022Quote: Candidate small RNA sequences were identified from raw sequence data using cutadapt (ver. 2.8)21 with parameters “-u -4 -a A(10)” as recommended by PerkinElmer NextFlex Combo-Seq kit ...
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bioRxiv - Cell Biology 2023Quote: ... quantitative microscopy was performed using the Operetta HCS imaging system with a 10×/0.3 NA objective (PerkinElmer, Courtaboeuf, France). Images were analyzed using scripts developed in Acapella software (PerkinElmer ...
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bioRxiv - Biochemistry 2023Quote: ... 10 μM purified IKK2 protein was first autophosphorylated in the presence of 100-200 nM of γ32P-ATP (PerkinElmer) in kinase assay buffer for 1 hour at 30°C ...
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bioRxiv - Cell Biology 2024Quote: ... increasing concentrations of carbachol (from 0.01 µM up to 10 µM) were used in presence of 200 pM [3H]N-Methylscopolamine ([3H]NMS; 78.9 Ci/mmol; PerkinElmer).
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bioRxiv - Cell Biology 2020Quote: ... mice were anesthetized with 2% isoflurane and renilla bioluminescence was visualized/quantified by the IVIS Spectrum In Vivo Imaging System (PerkinElmer, 5-minute medium binning) in CDP-UMCG ...
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bioRxiv - Cell Biology 2020Quote: ... the mice were anesthetized with 2% isoflurane and firefly bioluminescence was visualized/quantified using the IVIS Spectrum In Vivo Imaging System (PerkinElmer, 5-minute medium binning) in CDP-UMCG ...