Labshake search
Citations for PerkinElmer :
51 - 100 of 123 citations for 6 Amino m ethylphenol hydrochloride since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2020Quote: Morphological measurements of lumen volume (Figure 6) were performed using Volocity 6.3.1 (Perkin Elmer) where an object is defined by use of the built-in “findobjects” method and selecting an intensity value to set the threshold for volumetric regions based on the channel used ...
-
bioRxiv - Cell Biology 2021Quote: ... Images were acquired at 1 frame/6-10 s and analyzed using Volocity (PerkinElmer) and NIH ImageJ ...
-
bioRxiv - Cell Biology 2021Quote: AlphaLISA™ Immunoassay was used to assess the IL-6 cytokine release (PerkinElmer, #AL223) in the media in 2D and 3D fibroblast cell cultures ...
-
bioRxiv - Cancer Biology 2022Quote: ... Image analysis was performed using Volocity 6 or 6.3 image processing software (Perkin Elmer).
-
bioRxiv - Cell Biology 2019Quote: ... 6 μM mRNA and 0.3 μM [35S]-fMet-tRNAfMet ([35S]-methionine from Perkin Elmer). An aliquot (4.5 μl ...
-
bioRxiv - Cell Biology 2021Quote: ... washed cells were pelleted, resuspended in 226 μl of Digestion Solution (200 μl 1.2 M sorbitol-citrate, 20 μl glusulase (PerkinElmer), 6 μl 10 mg/ml zymolyase (AMS Biotechnology) ...
-
bioRxiv - Biochemistry 2020Quote: ... Cells were lysed in 0.5 mL of 0.05 M NaOH and 0.4 mL were measured by scintillation counting with a Tri-Carb2910TR counter (Perkin Elmer). The remaining cell lysate was used to determine protein content using a Bradford protein assay ...
-
bioRxiv - Plant Biology 2020Quote: ... Two 100-μl fractions were collected in 50 μl of 1.5 M Tris-HCl (pH 8.0) and 1 ml of Ultima Gold LLT scintillation cocktail (Perkin Elmer). Radioactivity was measured using a liquid scintillation analyser (Packard).
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... In order to quantify IL-6 and TNF-α LANCE® Ultra Detection Kits (PerkinElmer, Inc ...
-
bioRxiv - Cell Biology 2023Quote: ... Imaging was performed on an Ultraview Vox spinning disk confocal system running Volocity 6 (PerkinElmer) using a standard inverted microscope (IX81 ...
-
bioRxiv - Cancer Biology 2019Quote: ... 10 nmol of chemerin-9 in 25 μL iodination buffer (0.5 M sodium phosphate, pH 7.4) were mixed with 1 mCi carrier-free Na125I (NEZ033L010MC, PerkinElmer, Waltham, USA) in an HPLC glass vial with microvolume insert ...
-
bioRxiv - Cell Biology 2021Quote: ... buffer and 1.2 M sorbitol citrate solution, and then digested by 1.4 mg/ml 20T zymolyase (amsbio, 120491) and glusulase (PerkinElmer, NEE154001EA). Cells were spread on poly-L-lysine (Sigma ...
-
bioRxiv - Molecular Biology 2020Quote: ... A LANCE Ultra TR-FRET kinase assay was used for measurements of enzyme activities that included an-Ulight labeled (Thr-1342) peptide substrate (catalog #TRF0130-M, PerkinElmer) and a Europium-labeled anti-phospho peptide antibody (catalog # TRF0218-M) ...
-
bioRxiv - Molecular Biology 2022Quote: ... RNA samples were separated on a 7 M urea denaturing polyacrylamide gel at 4°C at 100V and transferred onto a charged nylon transfer membrane (PerkinElmer), followed by cross-linking using a UV Stratalinker and drying at 80°C on a Gel Dryer (Bio-Rad).
-
bioRxiv - Microbiology 2023Quote: ... Samples were mixed with the DNA absorption solution (for one sample: 50 uL 5 M NaCl, 15 ul magnetic beads (GMG-252-A-100mL – PerkinElmer), 250 uL 7.8 M guanidium chloride ...
-
bioRxiv - Cell Biology 2020Quote: ... Cells were cultured for 6 days and cell proliferation was measured using ATPlite Luminescence Assay (PerkinElmer).
-
Dimeric prion protein ligand activates Adgrg6 but does not rescue myelinopathy of PrP-deficient micebioRxiv - Neuroscience 2020Quote: ... The reaction was stopped with 0.5 M H2SO4 and absorbance at 450 nm was measured in a plate reader (Perkin Elmer, EnVision). The experiment was performed with technical duplicates.
-
bioRxiv - Biophysics 2020Quote: ... 100 nM hNatB was mixed with a saturating concentration of “MDVF” peptide substrate (500 μM) and varying concentrations (1.95 μM to 1 mM) of acetyl-CoA (14C-labeled, 4 mCi mmol−1; PerkinElmer Life Sciences) for 10-minute reactions ...
-
bioRxiv - Cancer Biology 2021Quote: ... with 15 µl of sodium phosphate buffer (0.5 M, pH 7.6) was mixed with 37 MBq carrier-free Na125I (NEZ033L010MC, Perkin Elmer, Waltham, US). 4 μl chloramine T (1 mg/ml ...
-
bioRxiv - Microbiology 2022Quote: ... The production of LepB–SecM and LepB–CutF variants was induced by the simultaneous addition of 0.1 M IPTG and 2 µl of L-[35S] methionine-cysteine (7 mCi/ ml, PerkinElmer Life Sciences) (38) ...
-
bioRxiv - Biochemistry 2023Quote: ... 5 μL of 40 μM SFK peptide was added to each well and raw fluorescence units were measured immediately on an Envision fluorometer (Perkin Elmer) with an excitation wavelength of 344 nm and an emission wavelength of 405 nm in real time for the first 15 min at 15 s intervals ...
-
bioRxiv - Cell Biology 2023Quote: ... and 4,000 cells/well (for G2/M phase nocodazole treatment) with a complete media overnight onto poly-D-lysine-coated CellCarrier-Ultra-96 well Black (Perkin Elmer).
-
bioRxiv - Microbiology 2023Quote: ... the labeled sialyl-lactose was eluted with 1 M ammonium formate and quantified by scintillation counting using a Tri-Carb 2810 TR counter (Perkin Elmer).
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... with 15 μL of sodium phosphate buffer (0.5 M, pH 7.6) was mixed with 37 MBq carrier-free Na125I (NEZ033L010MC, Perkin Elmer, Waltham, US). 4 μl chloramine T (1 mg/mL ...
-
bioRxiv - Immunology 2023Quote: ... Iodine-125 (125I) labeling was performed by incubating DTPA-bsAbs in iodogen coated Eppendorf tubes with Na125I (10−5 M NaOH, Perkin Elmer) in 0.5 M phosphate buffer (pH 7.4 ...
-
Transcription factor LSF facilitiates lysine methylation of α-tubulin by microtubule-associated SET8bioRxiv - Biochemistry 2019Quote: ... or recombinant MBP-β-tubulin were incubated with 6 μM radioactively labeled [3H] AdoMet (Perkin Elmer #NET155V001MC) in 1X HMT buffer containing 5 mM Tris pH 8.0 ...
-
bioRxiv - Pathology 2021Quote: ... 6 min prior to imaging using an in-vivo imaging system (IVIS; Perkin Elmer, Waltham, MA, USA) as mentioned in our work (46).
-
bioRxiv - Cell Biology 2021Quote: Time-lapse or live-cell fluorescence microscopy was performed using a Zeiss Axiovert 200 M microscope (Plan Apochromat 1006, 1.4NA objective) with an Ultra-View RS-3 spinning disk confocal system (PerkinElmer Inc., United States) which equipped with a CSU21 confocal optical scanner ...
-
bioRxiv - Cancer Biology 2021Quote: ... the PFA-fixed sections were blocked and permeabilized in TNBT (0.1 M Tris, pH 7.4, 150 mM NaCl, 0.5% blocking reagent from Perkin Elmer, 0.5% Triton X-100) for 4h at room temperature ...
-
bioRxiv - Neuroscience 2020Quote: ... Reaction termination and detection were performed using 1X LANCE detection buffer with 20 mM EDTA and 2 nM LANCE Ultra Europium anti-phospho Glycogen Synthase (Ser641) (Perkin Elmer #TRF0220-M).
-
bioRxiv - Cancer Biology 2020Quote: ... pR248W concentration was determined by absorbance measurements at 280 nm (ε = 5690 cm−1 M−1 for tryptophan) on a Lambda 25 UV/Vis Spectrophotometer (PerkinElmer, Waltham, MA) using quartz cuvettes (1 cm path-length).
-
bioRxiv - Cancer Biology 2022Quote: ... Addition of a Europium-labelled antibody specific to the phosphopeptide (LANCE Ultra Europium-anti-phospho-Crosstide (anti-GSK-3α Ser21, Perkin Elmer, TRF0202-M) allows proximity-dependent energy transfer from the Europium donor to the Ultra UlightTM acceptor ...
-
bioRxiv - Cancer Biology 2021Quote: ... Number of basal and suprabasal cells in each clone was counted using Volocity 6 Image Analysis software (PerkinElmer).
-
bioRxiv - Neuroscience 2020Quote: HUVECs were incubated for 6 hours in EGM-2 containing 0.4 μCi/ml [5-3H]-D-glucose (PerkinElmer). Supernatant was transferred into glass vials containing perchloric acid and sealed with rubber stoppers ...
-
bioRxiv - Cell Biology 2022Quote: ... Hepatocytes were seeded into 6-well (300,000 per well) and 384-well microplates (1,000 per well, CulturPlate, PerkinElmer) precoated with collagen (rat tail ...
-
bioRxiv - Microbiology 2020Quote: ... A subsample of oven-dried soil was sieved with a 500 μm mesh and analyzed for total carbon and total nitrogen (TC, TN) using an elemental analyzer (2400 CHNS Analyzer; Perkin Elmer; Waltham, Massachusetts, USA) at the Environmental and Agricultural Testing Service laboratory (Department of Crop and Soil Sciences at NC State) ...
-
bioRxiv - Cell Biology 2019Quote: ... with a 60X 1.42 NA oil-immersion objective coupled to an Ultraview Vox spinning disk confocal system running Volocity 6 (PerkinElmer). Images were captured with a EMCCD camera (C9100-13 ...
-
bioRxiv - Bioengineering 2019Quote: ... and 6 post-infection was performed via radiographs with fluorescent overlays using the IVIS Lumina XRMS II system (PerkinElmer).
-
bioRxiv - Biochemistry 2019Quote: ... Plates were then washed 6 times with PBST and buffer containing PBS and 0.2 µg/ml Eu3+-streptavidin (PerkinElmer) and incubated at room temperature for 1 hour ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... The cells were lysed with NaOH (1 M, 1 mL) and transferred to RIA tubes for counting in a γ-counter (Perkin Elmer, Wallac Wizard 1480). The uptake and internalized fraction of the radiotracers were expressed as the percentage of total added activity to each well.
-
bioRxiv - Cancer Biology 2020Quote: ... the two replicates of the 6 h treatment time point for the H1299 cell line were acquired on an equivalent Opera Phenix imaging system (PerkinElmer). Quantitative analysis of the data was carried out using the metrics and analysis methods detailed below ...
-
bioRxiv - Molecular Biology 2019Quote: ... For micro-CT irradiation cells in 6 cm culture dishes or in 1.5 ml microcentrifuge tubes were scanned (Quantum FX, Perkin Elmer) in the low resolution mode (73 mm f.o.v. ...
-
bioRxiv - Biochemistry 2021Quote: ... and 640 μM) were added along with 10 μM of non-radiolabeled UTP plus 6 mCi of [α32P]-UTP (PerkinElmer). Upon addition of nucleotides ...
-
bioRxiv - Neuroscience 2020Quote: ... 3 and 6 hr later cells were labeled for 5 min with 1 mCi/ml 35S Protein Labeling Mix (PerkinElmer). Lysates from 25-ml culture triplicate samples were analyzed by SDS-PAGE and autoradiography.
-
bioRxiv - Immunology 2020Quote: ... during the final 6 hours (murine MLR) or 15 hours (human MLR) of culture was measured by a TopCount (PerkinElmer).
-
bioRxiv - Cell Biology 2019Quote: GBM2 cells were plated at 2000 cells/well and exposed to Prestwick compounds (3 µM; Table 6) for 3 days in 384-well optical bottom assay plates (PerkinElmer). Cells were then fixed and stained with rabbit polyclonal anti-H3K27ac and mouse monoclonal anti-H3K27me3 antibodies followed by AlexaFluor-488- or AlexaFluor-555-conjugated secondary antibodies ...
-
bioRxiv - Cancer Biology 2021Quote: 4T1 cells were cultured for 6 h in RPMI medium containing 0.4 μCi ml−1 [5-3H]D-glucose (Perkin Elmer) after which the supernatant was transferred into glass vials sealed with rubber stoppers ...
-
bioRxiv - Microbiology 2022Quote: ... maintained at 200 °C and with N2 as the gas vector (flow rate of 6 mL·min-1) with a flame ionization detector (FID) maintained at 280 °C (PerkinElmer, USA)
-
bioRxiv - Microbiology 2022Quote: ... OD600 and luminescence measurements were taken either 1-hour post-resuspension or over 6 hours starting at 0.5-hour post-resuspension using the Victor X3 2030 multilabel plate reader (Perkin Elmer). Luminescence was measured in counts per second (CPS ...
-
bioRxiv - Microbiology 2023Quote: ... cells were cultured in 6-well plates and metabolically labeled (Easytag Express 35S-Met-Cys protein labeling mix (Perkin Elmer)) with 0.2 mCi/ml for various times ...