Labshake search
Citations for PerkinElmer :
201 - 250 of 1200 citations for 6β Fluoro 21 hydroxypregna 1 4 9 11 16 tetraene 3 20 dione 21 acetate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biophysics 2023Quote: ... 100 µL of MicroScint-20 (Perkin Elmer, #6013621) were added to each well and the plate was shaken at room temperature for 60-90 min ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... 4 μL/well of ATPLite (PerkinElmer) was added to assay plates and incubated for 15 min at room temperature ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... 4 μL/well of ATPLite (PerkinElmer) was dispensed ...
-
bioRxiv - Cell Biology 2020Quote: ... 4 μl/well of ATPLite (PerkinElmer) was added to assay plates and incubated for 15 min at room temperature ...
-
bioRxiv - Cell Biology 2019Quote: GBM2 cells were plated at 2000 cells/well and exposed to Prestwick compounds (3 µM; Table 6) for 3 days in 384-well optical bottom assay plates (PerkinElmer). Cells were then fixed and stained with rabbit polyclonal anti-H3K27ac and mouse monoclonal anti-H3K27me3 antibodies followed by AlexaFluor-488- or AlexaFluor-555-conjugated secondary antibodies ...
-
bioRxiv - Neuroscience 2020Quote: ... TR-FRET readout was performed after 1 h incubation at 4°C using an EnVision 2105 Multimode Plate Reader (PerkinElmer) with previously defined measurement parameters53 ...
-
bioRxiv - Neuroscience 2022Quote: ... 022-105721-00321-0001, POLATOM, as a marker for K+ transport, and 4 μCi ml-1 3H-mannitol as an extracellular marker, PerkinElmer) for 2min (influx ...
-
bioRxiv - Molecular Biology 2021Quote: HDL-sRNA libraries were generated using the NEXTFlex Small RNA-Seq Kit v3 with 1:4 adapter dilution and 22 PCR cycles (PerkinElmer). Following PCR amplification ...
-
bioRxiv - Molecular Biology 2022Quote: ... was next added and incubated with the mixture at 4 °C for 1 hour before the measurements performed on Envision Readers (PerkinElmer). The fluorescently-labeled m6A-containing mRNA was used to adjust the gain factor ...
-
bioRxiv - Molecular Biology 2024Quote: ... membranes were blocked with 5% BSA in Tris / HCl-buffered saline / 0.05% Tween (TBST) for 24 h at 4 °C and afterwards membranes were incubated for 1 h with HRP-Streptavidin (PerkinElmer; #NEL750001EA ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... 1,200 cells/16 μL/well of CCD-18co cells were seeded in CellCarrier-384 Ultra microplates (Perkin Elmer, Cat #: 6057302) with an EL406 combination microplate washer dispenser (EL406 ...
-
bioRxiv - Developmental Biology 2021Quote: ... 3-D reconstruction was performed with Volocity (PerkinElmer).
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... 3-plex positive control probe or with a cocktail of Abcb5-C1 and Abcb4-C2 probes with a 1:750 dilution of TSA-Fluorescein Plus and TSA-Cyanine 3 Plus (PerkinElmer, Shelton, CT), respectively ...
-
bioRxiv - Cancer Biology 2024Quote: ... 1:3 dilution steps) using a Janus Gripper with 384-channel Modular Dispense Technology dispensing head (revvity, previously Perkin Elmer, MA, USA). Afterwards ...
-
bioRxiv - Immunology 2021Quote: ... were plated in 3 ml of complete medium supplemented with IL-2 (20 IU/ml) and incubated with 1 μl of 3H-labeled ROL (PerkinElmer, Boston, MA, USA) for 4 h ...
-
bioRxiv - Cancer Biology 2022Quote: ... Organoid/hydrogel cultures were serum starved overnight before treatment with EGF (20 ng/mL) and promegestone/R5020 (1, 10, 100 nM; Perkin Elmer, Cat. #: NLP00400) and 10 nM R5020 with or without the addition of FAK (1 μM ...
-
bioRxiv - Developmental Biology 2021Quote: ... Samples were washed again in PBS with 0.1% Tween-20 six times for 1 hour and incubated in fluorescein tyramide reagent (Perkin Elmer, Cat. No. SAT701001EA) diluted 1:70 in amplification diluent for 50 minutes at room temperature ...
-
bioRxiv - Molecular Biology 2021Quote: ... 2µl (80U) RNasin and 20 µCi γ32P-ATP (PerkinElmer) were added to the beads pellet and incubated 60 min at 37°C ...
-
bioRxiv - Neuroscience 2022Quote: ... 50 μl of scintillation fluid (MicroScint™-20; PerkinElmer) was added ...
-
bioRxiv - Biochemistry 2022Quote: ... cells were harvested with 20 μL Britelite Plus (PerkinElmer), and luminescence was measured on a BioTek Synergy Neo multimode plate reader ...
-
bioRxiv - Molecular Biology 2024Quote: ... dGTP and 20 μCi of α-32P-dATP (PerkinElmer) and incubated for 2h at 37°C as reported in 13 ...
-
bioRxiv - Neuroscience 2023Quote: ... of incubation in an isotope solution containing rubidium (86Rb+) (1 μCi/ml, 022-105721-00321-0001, POLATOM) and 3H-mannitol (4 μCi/ml, NET101, Perkin Elmer). 86Rb+ acts as a K+ congener ...
-
bioRxiv - Neuroscience 2023Quote: ... Thawed serum samples were diluted with 1xPBS 1:4 and assayed in triplicates using a fluorometer (PerkinElmer, VICTOR3V 1420 Multilabel counter) with excitation/emission of 485 nm / 535 nm (25 nm band-pass filter) ...
-
bioRxiv - Cancer Biology 2023Quote: ... Following an HRP conjugation step a TSA Opal 570 1:150 (Tyramide Signal Amplification Opal 4-Color Manual IHC Kit, Perkin Elmer) was linked to the IRF7 antibody previously bound to the slide overnight ...
-
bioRxiv - Immunology 2020Quote: ... often from an ImmPRESS® polymer detection kit followed by colour development using a tyramide signal amplification system kit with cyanine (Cy)3 or fluorescein (1:50 dilution; PerkinElmer, Waltham, MA, USA). For detection of the second antigen in dual immunofluorescence ...
-
bioRxiv - Molecular Biology 2022Quote: U10 and CUGCUGUUUU (20 pmol) were chemically synthesized (Integrated DNA Technologies (IDT)) and 20 pmol RNA was radioactively labeled using [γ-32P]-ATP (PerkinElmer, 10mCi/ml) and 10 units T4 Polynucleotide Kinase (PNK ...
-
bioRxiv - Biochemistry 2021Quote: ... The lysate was mixed with Optiphase Hisafe 3 (PerkinElmer), and radioisotope activity was monitored by LSC-8000 β-scintillation (Hitachi) ...
-
bioRxiv - Neuroscience 2020Quote: ... then incubated in TSA plus cyanine 3 (Perkin Elmer) for 10 min ...
-
bioRxiv - Cancer Biology 2019Quote: ... A Vectra 3 pathology imaging system microscope (PerkinElmer, Inc.) was used to obtain images ...
-
bioRxiv - Neuroscience 2020Quote: ... The luminescent intensity was measured with VICTOR 3 (PerkinElmer), and the values were normalized to the MAO B protein level measured by Western blotting.
-
bioRxiv - Neuroscience 2019Quote: ... Confluent cultures were changed by quiescent medium for 24 h before they were labelled for 4 h with 1 μCi/ml [3H] arachidonic acid (PerkinElmer Life Sciences). Washing cells once with PBS containing 0.1% free fatty acid albumin and twice with PBS alone ...
-
bioRxiv - Biophysics 2021Quote: ... 50 nM SpNatC (WT or mutants) was mixed with 50 μM Ac-CoA (14C-labeled, 4 mCi mmol-1; PerkinElmer Life Sciences) and varying peptide concentrations (ranging from 1.95 μM to 500 μM ...
-
bioRxiv - Neuroscience 2023Quote: ... The quality of the RNA in a randomly selected representative number of wells (16 out of 92 wells) was analyzed by microcapillary electrophoresis on a Labchip GX Touch instrument (PerkinElmer Catalog# CLS138162), and plates with RNA samples with a RIN value consistently ≥7 and free of DNA contamination were used for cDNA synthesis ...
-
bioRxiv - Neuroscience 2021Quote: ... Sections were incubated for 10 min in Cy3 Plus Amplification Reagent diluted 1:300 in 1x Plus Amplification Diluent (TSA Plus Cyanine 3 System, Perkin Elmer, Waltham, MA, NEL744001KT), washed several times in TBS with 0.3% Triton X-100 and 0.05% Tween 20 ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... Ligand-stimulated cAMP accumulation in the presence or absence of 0.5 mM 3-isobutyl-1-methylxanthine (IBMX) was measured after the indicated times of stimulation using LANCE® cAMP Detection Kit (Perkin Elmer Life Sciences) and a Mithras LB 940 multimode microplate reader ...
-
bioRxiv - Biophysics 2019Quote: ... cells were harvested with 20 μL Britelite Plus (Perkin Elmer), and luminescence was measured on a BioTek Synergy Neo multimode plate reader ...
-
bioRxiv - Genomics 2021Quote: ... was labelled with γ-32P ATP (20 μCi) (Perkin Elmer) in a 25 μL reaction containing 1X PNK buffer and 15 units of polynucleotide kinase (New England Biolabs ...
-
The membrane-cytoplasmic linker defines activity of FtsH proteases in Pseudomonas aeruginosa clone CbioRxiv - Biochemistry 2023Quote: ... 20 µCi/mL EasyTag Express 35S Protein Labeling Mix (PerkinElmer) was added to cultures and temperature was reduced to 30°C ...
-
bioRxiv - Neuroscience 2021Quote: ... after 1 hour at 37°C the reaction was stopped with 0.25 M glycine buffer pH 10.4 and the fluorescence emission of the 4-methylumbelliferyl generated (4-MU) by the reaction was read with a fluorimeter (EnSpire Plate Reader, PerkinElmer) selecting an excitation wavelength of 365 nm and an emission wavelength of 445 nm ...
-
bioRxiv - Cancer Biology 2019Quote: ... the Opal 4-colour IHC kit (Perkin Elmer) was used per manufacturer’s protocol.
-
bioRxiv - Molecular Biology 2022Quote: ... 4 µl of [γ-32P] ATP (PerkinElmer, #BLU502A001MC), and 10 units of T4 polynucleotide kinase (PNK ...
-
bioRxiv - Biochemistry 2022Quote: ... 4 μL of the acceptor beads (Perkin Elmer) diluted in PBS was added to each well in the dark and incubated for 90 mins ...
-
bioRxiv - Biochemistry 2022Quote: ... 4 μL of the donor beads (Perkin Elmer) diluted in PBS was added to each well to give a total volume of 25 μL and incubated for 2 hours ...
-
bioRxiv - Neuroscience 2020Quote: ... and a Wallac 1420 VICTOR 3 V luminometer (Perkin Elmer) according to the manufacturer’s protocol.
-
bioRxiv - Developmental Biology 2020Quote: ... and DLX5 were carried out using Cyanine 3 (PerkinElmer NEL752001KT) diluted 1:500 in RNAscope Multiplex TSA Buffer ...
-
bioRxiv - Cell Biology 2020Quote: ... A TSA Cyanine 3 Signal Amplification System (#SAT704A001EA, Perkin Elmer) was used for signal detection according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2020Quote: ... TSA Cyanine 3 or Cyanine 5 amplification kits (Perkin Elmer) were used according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2022Quote: ... Cyanine-3 (Cy3 Direct FISH; PerkinElmer Life Sciences, Waltham, MA) was used to visualize labeled cells and nuclei were counterstained with DAPI (ThermoFisher Scientific).
-
bioRxiv - Microbiology 2023Quote: ... The filters were soaked in 3 mL ULTIMA Gold (PerkinElmer) overnight before liquid scintillation counting ...
-
bioRxiv - Neuroscience 2022Quote: ... TSA fluorophores for cyanine 3 and cyanine 5 (Perkin Elmer) were used at a concentration of 1:1000 ...