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Citations for PerkinElmer :
401 - 450 of 1377 citations for Dengue Virus Serotype 4 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... The radioactive counts (cpms) of immunoprecipitated protein was quantified using a 96-well Microbeta Trilux liquid scintillation plate reader (Perkin Elmer). Antibody index for each sample was calculated as follows ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were pulse labeled for 15 minutes with 132 μCi/6 cm dish with EasyTag Express 35S Protein Labeling Mix (Perkin Elmer). Radiolabeling was stopped by adding excess ...
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bioRxiv - Cell Biology 2023Quote: ... or ECL anti-rabbit antibodies (NA934V; 17434242) (Amersham) were used for detection of probed proteins using enhanced chemiluminescence (ECL)(Perkin Elmer). Chemiluminescence was detected using an iBright FL1500 (ThermoFisherScientific ...
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bioRxiv - Cancer Biology 2023Quote: ... then each well was incubated for 30 min at 37 °C with 5 μl/well EXPRE35S35S Protein Labeling Mix (PerkinElmer NEG072002MC), after which cells were lysed in RIPA lysis buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: The recruitment of β-arrestin2 by MOR agonists after G protein activation was assessed using the Cisbio HTRF-based β-arrestin2 recruitment assays (Cisbio Assays, PerkinElmer), which monitored the interaction between β-arrestin2 and AP2 ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were pulsed with 0.1 mCi/ml 35S-L-methionine and 35S-L-cysteine mix (EasyTag™ EXPRESS35S Protein Labeling Mix, Perkin Elmer) in methionine- and cysteine-free DMEM for 15 min ...
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bioRxiv - Molecular Biology 2021Quote: ... HeLa cells (5×104 cells/ml) were adhered in an Cellcarrier Ultra optical 96-well plate (PerkinElmer). Cells were washed three times with ice-cold PBS and fixed in 4% (vol/vol ...
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bioRxiv - Cancer Biology 2020Quote: ... 5 × 102 cells were seeded on cell culture-coated 96-well white-walled CulturePlates (Perkin Elmer #6005688). Cells were lysed with CellTiter-Glo® 2.0 Reagent (Promega) ...
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bioRxiv - Molecular Biology 2022Quote: CHO-K1 cells were seeded at a density of 30,000 cells/well in Isoplate-96 plates (PerkinElmer). The WT GIPR or SVs were transiently transfected using Lipofectamine 2000 transfection reagent ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were then seeded at 2000 cells per well in CellCarrier Spheroid Ultra-low attachment microplates (PerkinElmer) in enriched pseudoislet media as described59 ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell segmentation and phenotyping of the cell subpopulations were performed using the inForm software (PerkinElmer Inc., USA). The frequency of all immune cell populations analyzed and the cartographic coordinates of each stained cell type were obtained ...
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bioRxiv - Molecular Biology 2024Quote: siRNA-transfected or Dexamethasone-synchronized cells were plated in 96-well Cell Carrier Ultra plates (Perkin Elmer). At the end of DSB inducing treatment ...
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bioRxiv - Evolutionary Biology 2019Quote: ... animals were incubated overnight with peroxidase-conjugated antibodies at 4°C (anti-DIG-POD, Roche 1:500 dilution and anti-DNP, Perkin Elmer, 1:200 dilution) followed by the amplification of the signal with fluorophore-conjugated tyramides (1:100 TSA reagent diluents ...
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bioRxiv - Biochemistry 2021Quote: ... an optimized set of 4 small interfering RNAs (siRNAs; SMARTPool) targeted against human Furin were purchased from Horizon Discoveries (Perkin Elmer, Lafayette, LA, USA) and transfections of HeLa cells were carried out using INTERFERin (PolyPlus ...
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bioRxiv - Cancer Biology 2021Quote: ... For multiplexed immunohistochemistry signals were detected using Opal™ 4-Plex Kit according to the manufacturer’s protocol (catalog no. NEL810001KT; Perkin Elmer, Waltham, MA, USA). For more details see Supplemental Materials section.
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bioRxiv - Cancer Biology 2020Quote: ... proteins were first labeled for 48 h with 0.25 μM Ci ml-1 L-[U-14C] VALINE (Perkin Elmer, Waltham, MA, USA) in GIBCO-RPMI 1640 medium (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... The precipitates proteins were resolved by SDS-PAGE and [32P]-labeled proteins was visualized using a Super Resolution screen (Perkin Elmer and quantified using a Cyclone Plus Storage Phosphor System (Perkin Elmer). The signal was normalized to the relative cellular content as determined by immunoblotting of whole cell lysates probed with antibodies.
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bioRxiv - Cell Biology 2021Quote: ... Horseradish peroxidase (HRP) conjugated secondary antibody was added in order for labelled proteins to be detected by Western Lightning Plus-ECL (PerkinElmer, 203-170071). Primary and secondary antibodies used are listed in Supplementary Table 5.
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bioRxiv - Cell Biology 2022Quote: ... HEK293T cells transfected with FTTTRA25T and were labeled for 30 min with EasyTag Express [35S] Protein Labeling Mix (0.1 mCi/mL; Perkin Elmer CAT# NEG772014MC) in DMEM lacking both Cys and Met (Gibco CAT# 21013024 ...
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bioRxiv - Molecular Biology 2022Quote: ... Membranes were finally washed three times with PBST for 5 min and protein levels were visualized by enhanced chemiluminescence (Perkin Elmer, NEL104001EA) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... Membranes were washed at room temperature 3 times 10 min in PBST before using chemiluminescence to reveal protein bands with the Western Lightning® Plus-ECL Enhanced Chemiluminescence reagent (PerkinElmer Inc.) as per the manufacturer’s instructions.
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bioRxiv - Cell Biology 2022Quote: Confluent 6-well dishes of FRT cells (approximately 1×106/well) were metabolically labeled in DMEM depleted of methionine and cysteine and supplemented with EasyTag 35S Protein Labeling Mix (Perkin Elmer, NEG772007MC), glutamine ...
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bioRxiv - Molecular Biology 2023Quote: ... 6 ng/ µl recombinant TopBP1 protein or buffer were added to TopBP1 immunodepleted interphase Xenopus egg extract containing 50 nCi/µl α32P-dCTP (Perkin Elmer NEG513A250UC). To start the replication reaction the egg extracts were supplemented with 5 ng sperm DNA (kindly provided by the lab of O ...
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bioRxiv - Cell Biology 2019Quote: ... Live cell imaging of cells was performed with a Nikon/Perkin Elmer Ultra VIEW VoX system (Perkin Elmer). Mitochondrial morphology of bafilomycin-treated cells was assessed as described (Fonseca et al. ...
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bioRxiv - Neuroscience 2021Quote: ... cells were seeded in either 12-well plates (0.1× 106 cells) or 96-well CellCarrier Ultra plates (8,000 cells, PerkinElmer) before transfecting with 40 nM ONTARGETplus Non-targeting oligo siRNA (NT1 siRNA ...
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bioRxiv - Biophysics 2022Quote: ... Cells were plated at 15.000 cells/well (for 24h seeding) or 10.000 cells/well (for 48h seeding) in 96-well PhenoPlates (PerkinElmer) that were coated with poly-L-lysine (Sigma ...
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bioRxiv - Systems Biology 2024Quote: ... The resuspended cells were then seeded at 1000 cells per well in 384-well white Optiplates (Perkin Elmer) and incubated with PTH(1–34 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... cells in CellCarrier-96 Ultra Plates (PerkinElmer) were incubated for 4 h with pseudotyped viral stock at the desired MOI in media ...
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bioRxiv - Immunology 2021Quote: ... with the Unifilter-96 Cell Harvester (PerkinElmer). 20 μL of MicroScint-20 (PerkinElmer ...
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bioRxiv - Immunology 2021Quote: ... with the Unifilter-96 Cell Harvester (PerkinElmer). 20 μL of MicroScint-20 (PerkinElmer ...
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bioRxiv - Bioengineering 2023Quote: ... cells were lysed using BriteLite (Perkin Elmer) reagent and luminescence was measured using a BioTek Synergy HT Microplate Reader (BioTek) ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were imaged with Opera Phenix (PerkinElmer) at 40X ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were then mounted with PermaFluor (PerkinElmer) prior to imaging ...
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bioRxiv - Neuroscience 2023Quote: Cells cultures in 96 well PhenoPlates (PerkinElmer) were fixed in 4% PFA in PBS for fifteen minutes at RT ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were imaged using the OperaPhenix (PerkinElmer).
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bioRxiv - Genetics 2019Quote: ... were mounted on infrared windows where spectral images were collected at a 4 cm-1 spectral resolution and ~7 μm spatial resolution from a Spotlight 400 Imaging system (Perkin Elmer Instruments, Shelton, CT USA). Background spectra were collected under identical conditions from clear Ba2F windows and subtracted from sample data by instrumental software ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then incubated with a D1R antagonist for 1h at 4°C in a solution equivalent to the pre-incubation buffer with the addition of 1nM [3H]SCH23390 (Perkin Elmer, USA, 85 Ci/mmol). Non-specific binding was determined by incubating sections under the same conditions with excess amount of the D1R ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then incubated with a D2R antagonist for 1h at 4°C in a solution equivalent to the pre-incubation buffer with the addition of 4nM [3H]raclopride (Perkin Elmer, USA, 60.1 Ci/mmol). Non-specific binding was determined by incubating sections under the same conditions with excess amount of the D2R ...
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bioRxiv - Genomics 2019Quote: ... Anti-rabbit or anti-mouse HRP conjugated antibodies were used to detect protein by chemiluminescence using ECL (Perkin Elmer, Western Lightning Plus ECL). Images were visualized by GeneSnap image analysis software (SynGene ...
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bioRxiv - Cancer Biology 2020Quote: ... containing 10% BSA and 3 µl [35S] EasyTag Express protein labelling mix (7 mCi, 259 MBq, 1175 Ci/mmol in 632 ml; Perkin Elmer, Espoo, Finland). After 2 h of incubation at 37 °C cells were washed twice with PBS ...
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bioRxiv - Biochemistry 2023Quote: ... Ten μl of these dilutions or 10 μl of G protein storage buffer without nucleotides were pipetted into the corner of a white opaque 96-well plate (Opti-Plate, PerkinElmer Life Sciences, 6005290). Ten 10 μl of 0.1 mM Coelenterezine 400a diluted in BRET buffer (140 mM NaCl ...
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bioRxiv - Microbiology 2020Quote: ... 293FT cells (2 × 104 cells) were plated in 96-well View Plates (Perkin Elmer Life Sciences, Waltham, MA, USA). Five hours later ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Live cell staining for high content image analysis: cells were added to a CellCarrier-96 Ultra Microplates (Perkin Elmer), 1000 cells/well ...
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bioRxiv - Immunology 2021Quote: ... Vero E6 cells were seeded at 20,000 cells/well in culture medium into white 96-well plates (PerkinElmer, 6005688) and cultured overnight at 37°C 5% CO2 ...
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bioRxiv - Cell Biology 2023Quote: Cells were seeded at 4,000 cells/well onto poly-D-lysine-coated CellCarrier-Ultra-96 well Black (Perkin Elmer). Phenol red-free DMEM/F12 (#11039-021 ...
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bioRxiv - Cancer Biology 2022Quote: ... Cell viability was determined by incubating the cell with a luciferin solution (150 μg/mL; Perkin Elmer, MA, USA) and quantification of the bioluminescent signal emitted in an EnVision plate reader (Perkin Elmer).
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bioRxiv - Cell Biology 2023Quote: ... INS-1E cells were seeded at 20,000 cells per well in a CellCarrier Ultra 96-well plate (Perkin Elmer). Cells were incubated for 24 h ...
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bioRxiv - Neuroscience 2023Quote: ... 5000 cells from each cell line were plated in a 96-well plate (PhenoPlate 96-well, black, Perkin Elmer) according to the layout in Figure 3A ...
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bioRxiv - Cancer Biology 2024Quote: ... Mesothelial clearance was performed by seeding MeT5A-GFP cells (15) at the density of 1.3x 104 cells/well into 384-well CellCarrier plates (PerkinElmer), pre-coated with 10μg/ml collagen type I (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2023Quote: ... The experiments were initiated by placing the choroid plexus in HEPES-aCSF containing 1 μCi/ml 86Rb+ (022-105721-00321-0001, POLATOM, as a tracer for K+ transport) and 4 μCi/ml 3H-mannitol (Perkin Elmer, as an extracellular marker [26]) at 37°C for 2 min in the absence or presence of 2 mM ouabain (O3125 ...