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Citations for PerkinElmer :
401 - 450 of 1387 citations for 4 Methoxy 2 3 3 4 5 Pentacb 13C12 99% 50 Ug Ml In Toluene since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: ... 5 nM CFTR was incubated with 0.5 mg/ml YSi streptavidin SPA beads (PerkinElmer) in the presence of varying concentrations of lumacaftor (at 1:1 molar ratio of cold and [3H] lumacaftor (6.4 Ci/mmol ...
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bioRxiv - Cell Biology 2021Quote: ... A mixture of 10 μl of 5 μg/ml Nickel-chelate acceptor beads (PerkinElmer) and 20 μg/ml streptavidin donor beads (PerkinElmer ...
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bioRxiv - Cell Biology 2020Quote: ... 5 microcuries (μCi) of [γ-32P]-ATP (3000 Ci/mmol, 10 mCi/ml) (PerkinElmer) in kinase buffer (20mM HEPES pH 7.5 ...
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bioRxiv - Molecular Biology 2023Quote: ... 5 μl of [α-32P]-dCTP (3,000 Ci/mmol, 10 mCi/ml; Perkin Elmer), 0.5 μM primer ...
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bioRxiv - Cancer Biology 2021Quote: ... For each PI3K isoform a 3 µL mixture (1:1) of enzyme/lipid substrate solution was added to a 384 well white assay plate (Perkin Elmer #6007299) containing 50 µL of test compound or DMSO only for untreated controls ...
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bioRxiv - Cell Biology 2020Quote: ... The blot was washed in TTBS for 3 times and the signal was developed using Western Lightening Plus-ECL kit (PerkinElmer, Waltham, MA) and detected on HyBlot CL X-ray films (Denville).
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bioRxiv - Molecular Biology 2021Quote: ... The plates were read at a wavelength of 460 nm using the VICTOR 3 V 1420 Multi-Label Microplate Reader (Perkin Elmer, USA). All reactions were carried out in triplicate using both antigenic peptides in separate experiments ...
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bioRxiv - Cell Biology 2021Quote: ... Imaging involving mNG-Bud8 (Figs. 3 & S2) was performed using a spinning disk confocal microscope (Ultra-VIEW VoX CSU-X1 system; Perkin Elmer-Cetus) equipped with a 100x /1.45 NA Plan-Apochromat Lambda oil immersion objective lens (Nikon) ...
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bioRxiv - Cell Biology 2021Quote: Time-lapse or live-cell fluorescence microscopy was performed using a Zeiss Axiovert 200 M microscope (Plan Apochromat 1006, 1.4NA objective) with an Ultra-View RS-3 spinning disk confocal system (PerkinElmer Inc., United States) which equipped with a CSU21 confocal optical scanner ...
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bioRxiv - Neuroscience 2021Quote: Live-cell imaging was performed at 37°C and with 3% CO2 using an Opera Phenix™ high content confocal microscopy system (Perkin Elmer). One hour prior to imaging ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 3-plex positive control probe or with a cocktail of Abcb5-C1 and Abcb4-C2 probes with a 1:750 dilution of TSA-Fluorescein Plus and TSA-Cyanine 3 Plus (PerkinElmer, Shelton, CT), respectively ...
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bioRxiv - Cancer Biology 2024Quote: ... 1:3 dilution steps) using a Janus Gripper with 384-channel Modular Dispense Technology dispensing head (revvity, previously Perkin Elmer, MA, USA). Afterwards ...
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bioRxiv - Microbiology 2019Quote: ... Collected cell batches on polycarbonate filters were directly transferred into 6 ml scintillation vials and mixed with 5 ml UltimaGold XR (Perkin Elmer) scintillation cocktail ...
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bioRxiv - Immunology 2022Quote: ... or 2 µCi/mL (66.67 nM final concentration) [9,10-3H]palmitate (Perkin Elmer) for assessment of glycolytic or FAO flux ...
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bioRxiv - Cancer Biology 2023Quote: ... blots were developed in 2 mL Western Lighting Plus chemiluminescent substrate (Perkin Elmer) and imaged on the ImageQuant LAS4000 (Cytiva).
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bioRxiv - Cancer Biology 2021Quote: ... 50% and 25% MIA PaCa-2 cells) were grown in CellCarrier Spheroid ULA 96-well Microplates (6055330, PerkinElmer, Waltham, MA), as described previously ...
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bioRxiv - Immunology 2023Quote: ... Virus–monoclonal antibody-containing medium was then aspirated from cells and 50 or 100 µl of a 1:2 dilution of SteadyLite Plus (PerkinElmer) or BioGlo (Promega ...
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bioRxiv - Biophysics 2021Quote: ... Cell fixation was performed 24h after transfection using 4% formaldehyde and cellular imaging was performed using an Operetta CLS (PerkinElmer). Three individual plate preparations were used as independent experiments for statistical significance (n=3) ...
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bioRxiv - Biophysics 2022Quote: ... incubated for 4 h at 37°C and fluorescent signal intensity was quantified using the EnSpire Multimode Plate Reader (PerkinElmer) at excitation and emission wavelengths of 540 and 612 nm respectively.
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bioRxiv - Neuroscience 2020Quote: ... TR-FRET readout was performed after 1 h incubation at 4°C using an EnVision 2105 Multimode Plate Reader (PerkinElmer) with previously defined measurement parameters53 ...
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bioRxiv - Neuroscience 2022Quote: ... To quantify α1-3;5GABAAR79 sections were incubated for 60 minutes at 4°C in 1nM [3H]flumazenil (Perkin Elmer, NET757001MC), or in 1nM [3H]flumazenil with 10 µM flunitrazepam (Sigma Aldrich ...
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bioRxiv - Molecular Biology 2021Quote: HDL-sRNA libraries were generated using the NEXTFlex Small RNA-Seq Kit v3 with 1:4 adapter dilution and 22 PCR cycles (PerkinElmer). Following PCR amplification ...
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bioRxiv - Molecular Biology 2022Quote: ... RNA samples were separated on a 7 M urea denaturing polyacrylamide gel at 4°C at 100V and transferred onto a charged nylon transfer membrane (PerkinElmer), followed by cross-linking using a UV Stratalinker and drying at 80°C on a Gel Dryer (Bio-Rad).
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Aerrane (Isoflurane, Baxter) inhalation was performed for anesthesia using the RAS-4 Rodent Anesthesia System (Perkin Elmer, Waltham, MA, USA) for experiments with IVIS Spectrum CT living imaging system (Perkin Elmer ...
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bioRxiv - Molecular Biology 2022Quote: ... was next added and incubated with the mixture at 4 °C for 1 hour before the measurements performed on Envision Readers (PerkinElmer). The fluorescently-labeled m6A-containing mRNA was used to adjust the gain factor ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were washed with PBS and then stored in PBS at 4°C until imaged in the Opera Phenix (PerkinElmer) at 40x magnification and images analysed using a Harmony software to assess protein expression and morphology (see supplementary).
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bioRxiv - Molecular Biology 2024Quote: ... membranes were blocked with 5% BSA in Tris / HCl-buffered saline / 0.05% Tween (TBST) for 24 h at 4 °C and afterwards membranes were incubated for 1 h with HRP-Streptavidin (PerkinElmer; #NEL750001EA ...
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bioRxiv - Pathology 2023Quote: ... double immunohistochemistry for paraffin sections (8 μm) was conducted by using the Opal 4 color manual immunohistochemistry (IHC) kit (NEL810001KT, PerkinElmer). The signal of p-MLC2 was amplified by the Opal 4 color manual IHC kit (NEL810001KT ...
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bioRxiv - Bioengineering 2024Quote: ... The plates were incubated for 4 h at 37°C and then the supernatant was removed and incubated with scintillation fluid (PerkinElmer) overnight at room temperature ...
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bioRxiv - Plant Biology 2023Quote: ... resuspended in 2 N NaOH and mixed with 5 volumes scintillation fluid (Ultima Gold™ XR, PerkinElmer) for 14C-CPM counting in a TRI-CARB liquid scintillation counter (PerkinElmer ...
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bioRxiv - Cancer Biology 2019Quote: ... cancer cells (1,000 per well) were incubated with 50 μl of 51Cr solution (activity 1 mCi/ml, PerkinElmer) plus 50 μl of RPMI medium with 10% FBS for 2 h at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... Cultures were grown until OD600 ∼ 0.02 and labeled with 50 μCi/ml 32P-orthophosphate (900 mCi/mmol; PerkinElmer). When OD600 reached 0.2-0.4 ...
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bioRxiv - Systems Biology 2023Quote: ... The NPCs were cultured in a 12-well dish to confluency in NPC media and then dissociated using Accutase for 5 minutes at 37°C to single cell resolution and plated on 5 µg/mL human laminin and 5µg/mL fibronectin-coated 384 well plates (Perkin Elmer Cat #6057308) at 12,000 cells/well in NPC media and 10 µM ROCKi ...
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bioRxiv - Bioengineering 2019Quote: ... were analysed based on the Mehlich-3 extraction procedure (Mehlich, 1984) preceding inductively coupled plasma optical emission spectroscopy (ICP-OES, Optima 800, Winlab 5.5, PerkinElmer Inc.,Waltham, MA, USA). Exchangeable acidity (H + Al ...
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bioRxiv - Microbiology 2021Quote: ... The volumes of internalised DNA in GFP expressing bacteria were estimated by 3-D image analysis performed using Volocity software (Perkin Elmer, Waltham, USA).
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bioRxiv - Cell Biology 2021Quote: ... 41.3 Ci mmol−1) and radiocarbon-labelled serotonin binoxalate (14C-serotonin; 54.0 mCi mmol−1) were purchased from Perkin Elmer (Waltham, Massachusetts, USA). Serotonin creatinine sulphate ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Donor and acceptor emission were measured at 3 min intervals (during a total of 66 min) at 37°C with an EnVision 2014 Multilabel Reader (PerkinElmer, Waltham, MA, USA) using a 340/60 nm excitation filter and 520/8 nm (acceptor ...
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bioRxiv - Developmental Biology 2020Quote: ... RNA hybridization were performed on dechorionated 20 hpf embryos with the tyramide amplification kit (TSA Plus Cyanine 3 System, Perkin Elmer, Boston, MA). Briefly ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... were generated in 3 dimensions and minimized by applying the MM2 energy-minimization method in ChemBio3D (ChemDraw® Professional v20, PerkinElmer Informatics, Inc). The minimized structures were overlaid by steric fields and the similarity was calculated in Discovery Studio Visualizer v21.1 (Dassault Systèmes Biovia Corp).
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bioRxiv - Cancer Biology 2023Quote: ... culture plates were incubated for 3 days after which they were imaged with an Operetta CLS high-content analysis system (Perkin Elmer, LIVE configuration) with the incubation chamber set to 37 °C and 5% CO2 ...
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bioRxiv - Cell Biology 2023Quote: ... Colorimetric or fluorimetric quantifications of the respective readouts were done in a multiwell plate reader (Wallac Victor 3 V, PerkinElmer, Waltham, MA, USA) and conducted in white 96-well plates.
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bioRxiv - Cell Biology 2020Quote: ... K562 cells (5×106/ml) were labeled with 200 μCi of 51Cr (PerkinElmer, Wellesley, MA). After labeling ...
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bioRxiv - Cell Biology 2019Quote: ... transferred to scintillation vials containing 5 ml of Opti-Fluor scintillation liquid (6013199, Perkin Elmer) and subjected to LSC.
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bioRxiv - Microbiology 2022Quote: ... The supernatant was mixed with 5 mL of Ultima Gold XR (Perkin Elmer, Waltham, Massachusetts). The count-per-minute was determined for 5 min for each sample ...
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bioRxiv - Cancer Biology 2023Quote: ... Immuno-complexes were incubated for 20 min at 30°C in 30 μl of kinase buffer with a final concentration of 50 μM ATP and [γ-32P] ATP (1 x 10−2 μCi/pmol; PerkinElmer). Reactions were stopped by adding 5X LB ...
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bioRxiv - Microbiology 2021Quote: ... The washed filters were dried for 30 min under a heat-lamp and placed in a 20-ml Borosilicate Glass Scintillation vial containing 5 ml of Ultima Gold F liquid scintillation cocktail (Perkin Elmer, MA, USA). 14C radioactivity retained on the filters was quantified using an LS6500 scintillation system (Beckman Coulter Inc. ...
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bioRxiv - Immunology 2020Quote: ... Plates were stored in the dark at 4°C prior to imaging using a 40x objective mounted on an Operetta High-Content Imaging System (Perkin Elmer) controlled by Harmony 4.1 software.
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bioRxiv - Cell Biology 2021Quote: ... diluted in buffer A to a final concentration of 4 nM) was added to a 384-well microplate (OptiPlateTM-384 Plus, Perkin Elmer) followed by the addition of 15 μL diluted proteins and 5 μL of the GFP antibody-conjugated acceptor beads (5x) ...
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bioRxiv - Biophysics 2020Quote: ... 100 nM hNatB was mixed with a saturating concentration of “MDVF” peptide substrate (500 μM) and varying concentrations (1.95 μM to 1 mM) of acetyl-CoA (14C-labeled, 4 mCi mmol−1; PerkinElmer Life Sciences) for 10-minute reactions ...
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bioRxiv - Immunology 2019Quote: ... After 4-6 washes with 1x TBST the membrane was treated with chemiluminescent substrate Western Lightning ECL Pro (Perkin Elmer; NEL120001EA) according to manufacturer’s instructions ...