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Citations for PerkinElmer :
401 - 450 of 738 citations for 3 hydroxy 20 oxopregn 5 en 21 yl acetate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: Huh-7 and Calu-3 cells were seeded in 96-well plate 96-CellCarrierUltra plates (Perkin Elmer, Hamburg, Germany), treated with ARN726 30 μM or GW6471 1μM and infected with SARS-CoV-2 1 MOI and 0.1 MOI respectively ...
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bioRxiv - Molecular Biology 2024Quote: Mice were deprived of food for 3 h before intravenous injection of L-[4,5-3H(N)]leucine (NET1166, PerkinElmer) at a dose of 1 μCi/g ...
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bioRxiv - Microbiology 2024Quote: ... Zn2+ and Mn2+ amounts were calculated from standard curves made with Pure Plus Multi-Element Calibration Standard 3 (0.5-100ppb, PerkinElmer). Metals amounts detected in the 30% HNO3 blank were subtracted from all samples ...
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bioRxiv - Cell Biology 2020Quote: ... K562 cells (5×106/ml) were labeled with 200 μCi of 51Cr (PerkinElmer, Wellesley, MA). After labeling ...
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bioRxiv - Biochemistry 2020Quote: ... transferred to a 7 ml scintillation vial with 5 ml scintillation fluid (Ultima Gold, PerkinElmer), inverted several times to mix ...
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bioRxiv - Molecular Biology 2020Quote: ... DNA or RNA oligonucleotides were 5′ radiolabeled with [γ-32P]ATP (PerkinElmer, 3000 Ci/mmol), using T4 Polynucleotide Kinase (New England Biolabs ...
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bioRxiv - Biochemistry 2020Quote: ... the 25-nt oligonucleotide was labeled at the 5’ end by γ-32P ATP (PerkinElmer) using T4 polynucleotide kinase (NEB).
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bioRxiv - Neuroscience 2021Quote: ... d(CH2)5 [Tyr(Me)2,Thr4,Orn8,(125I)Tyr9-NH2] (125I-OVTA, PerkinElmer, Inc.). An adjacent series of slides ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... cell lysate (5 μL/well) was transferred to a 384-well ProxiPlate (PerkinElmer, Melbourne, Australia) and added to the donor and acceptor reaction mix (2.5 μL/ well ...
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bioRxiv - Molecular Biology 2020Quote: ... oligos were either 5’-end labelled with [γ-32P]-ATP (3000 Ci/mmol, Perkin Elmer) and T4 PNK (Thermo Fisher ...
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bioRxiv - Cancer Biology 2022Quote: ... Plates were shaken for 5 minutes before enhanced luminescence readout (EnVision, Perkin Elmer, Shelton, CT). All values were normalized by internal controls contained on each plate and analyzed for EC20 determination using Pipeline Pilot ...
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bioRxiv - Microbiology 2022Quote: ... Images were collected during 5 min using an IVIS (In Vivo Imaging System) Spectrum (PerkinElmer). Mice were imaged at days 5 ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant was mixed with 5 mL of Ultima Gold XR (Perkin Elmer, Waltham, Massachusetts). The count-per-minute was determined for 5 min for each sample ...
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bioRxiv - Molecular Biology 2023Quote: ... 5 mM MgCl2 and 1 mM DTT) supplemented with 0.76 μM radioactively labelled AdoMet (PerkinElmer) for 4 h at 37 °C or overnight at 25 °C ...
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bioRxiv - Systems Biology 2024Quote: ... Plates were shaken for 5 minutes before enhanced luminescence readout (EnVision, Perkin Elmer, Shelton, CT). All values were normalized by internal DMSO controls contained on each plate and analyzed for EC20 determination using Pipeline Pilot ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: Samples were diluted 1/5 or 1/10 in 1X Immunoassay buffer (Cat.#AL000F; PerkinElmer). For calibration ...
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bioRxiv - Microbiology 2023Quote: ... The p24 concentrations in the culture supernatants were measured on day 5 post infection (PerkinElmer).
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bioRxiv - Biophysics 2024Quote: ... Preincubated membrane vesicles (30-40 μg) were allowed to bind 5 nM [3H]ryanodine (PerkinElmer) in a buffer containing 50 mM MOPS (pH 7.4) ...
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bioRxiv - Synthetic Biology 2023Quote: ... left at room temperature for 5 minutes after which 100 μL SteadyLite Plus (PerkinElmer, 6066759) was added to each well ...
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bioRxiv - Molecular Biology 2023Quote: ... 5 mM MgCl2 and 1 mM DTT) supplemented with 0.76 μM radioactively labelled AdoMet (PerkinElmer) for 4 h at 37 °C or overnight at 25 °C ...
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bioRxiv - Biochemistry 2023Quote: ... 100 pmol of DNA was incubated with 2 μL 5 mCi [γ32-P]ATP (PerkinElmer) and 4 μL T4 PNK (New England BioLabs ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were diluted 1/5 or 1/10 in 1X Immunoassay buffer (Perkin Elmer, #AL000F). A 2-fold serial dilution of imiglucerase (Genzyme ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... [3H]5-HT and [3H]1-methyl-4-phenylpyridinium ([3H]MPP+) were obtained from PerkinElmer, Inc (Waltham ...
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bioRxiv - Physiology 2024Quote: Prdx4 RNA oligo was labeled with [32P]ATP (adenosine 5’-triphospate, [γ-32P]; NEG002A; PerkinElmer) using T4 polynucleotide kinase (M0201 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: AMPK (α1β1γ1 or α1β2γ1) phosphorylation of FAM SAMS peptide (5’-FAM-HMRSAMSGLHLVKRR-COOH – Perkin Elmer) was followed using a Caliper EZ reader (Perkin Elmer) ...
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bioRxiv - Biochemistry 2024Quote: ... the linearized substrates were labeled at both 5′ ends using [γ-32P]-ATP (Perkin Elmer) and T4 polynucleotide kinase (NEB).
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bioRxiv - Cell Biology 2021Quote: ... The AlphaLISA™ assay was in 20 μL reactions (requiring only 2 μL cell culture media sample) in 384-well plate (6007290, PerkinElmer) following standard two-step no-wash protocol without any modifications ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The plates were shaken on an orbital shaker at 300 rpm for 20 min and then read on an Envision 2104 Multilabel reader (PerkinElmer). Experiments were repeated in triplicate and data was analyzed with Graphpad Prism version 8.00 ...
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bioRxiv - Microbiology 2020Quote: ... Plates were shaken and incubated in darkness for 20 minutes and the luminescence value of each well was read by a luminometer (EnVision, PerkinElmer). Percent inhibition was calculated by subtracting the luminescence values of each experimental data point from the average minimum signal obtained from positive control values and dividing by the difference between the average maximum signal negative control and the positive control ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Cells were then incubated for 18 h and harvested for luciferase activity quantified using Britelite Plus (Perkin Elmer; 20 µL) on a Synergy Neo plate reader (Biotek) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and 20 μl of the reaction mixture was incubated at 26°C for 1 h in a 384-well AlphaPlate (PerkinElmer). Subsequently ...
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bioRxiv - Microbiology 2020Quote: ... The membrane was washed in PBS containing 0.1% Tween-20 and then exposed to developing solution (Western Lighting Chemiluminescence kit, Perkin Elmer) according to manufacturer’s guidelines ...
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bioRxiv - Immunology 2021Quote: ... cell lysate was loaded onto a 96-well plate (Grenier-BioOne) and mixed with MICROSCINTTM-20 scintillation fluid (Perkin Elmer). Samples were then incubated at room temperature for 2 hours and incorporated radioactivity analyzed on a 1450 MicroBeta TriLux Microplate Scintillation and Luminescence counter (Perkin Elmer).
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bioRxiv - Cancer Biology 2022Quote: ... 15 or 10-20% SDS-PAGE gels and proteins were transferred onto PVDF membranes (0.45 μm, Perkin Elmer, Waltham, MA) and blocked with 5% BSA/TBS-T for 2 hours at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... The plate was incubated for 20 min at room temperature and read in a Victor X5 plate reader (Perkin Elmer), in luminescent mode ...
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bioRxiv - Biochemistry 2022Quote: ... 5 μL time points were taken and quenched with 1.33 mM EDTA then brought to 20 μL final volume for detection using 1 nM LANCE Ultra Europium anti-H3K4me1/2 antibody (TRF0402, PerkinElmer) and 50 nM LANCE Ultra Ulight-Streptavidin (TRF0102 ...
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bioRxiv - Genomics 2022Quote: ... 20 µl of the lysate was transferred in duplicate in a well of an OptiPlate-96 HB (PerkinElmer, Waltham, MA) and 100 µl of Luciferase Assay Reagent II was added in each well ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The plates were further incubated for 20 min at room temperature and counted using the Wallac TriLux Microbeta counter (PerkinElmer). The results were analysed using GraphPad Prism 9.1 ...
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bioRxiv - Biochemistry 2023Quote: ... Protein biosynthesis rates were determined by measuring the incorporation of radioactive methionine in auranofin-treated and untreated samples after protein precipitation with 20 % (w/v) trichloro acetic acid using a scintillation counter (Tri-Carb 2800TR, PerkinElmer). 55 µg of protein from pulse-labeled samples for analytical gels and 300 µg of protein from unlabeled samples for preparative gels were rehydrated into IPG strips (24 cm ...
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bioRxiv - Neuroscience 2024Quote: Free-floating sections were rinsed 3 times for 2 min each in 0.01 M PBS with 0.2% Triton X-100 and then were blocked for 20 min in TSA Blocking Reagent (Perkin Elmer). Sections were incubated with primary antibodies (Supplementary Table 2 ...
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bioRxiv - Physiology 2024Quote: ... 4 µL of homogenate was briefly incubated with each drug treatment (eight-fold serial dilution ranging from 8 µM to 62.5 nM) in a 0.1% DMSO/50 ppm tween-20/E2 solution in 96-well plates (Perkin Elmer 6005299). A solution of 30 µL PBS ...
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bioRxiv - Cell Biology 2022Quote: ... The membranes were washed in TBST at least 3 times and probed with Western Lightning Plus ECL reagent (PerkinElmer, NEL105001EA) and exposed to GE Healthcare Hyperfilm ECL (28906839).
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bioRxiv - Cancer Biology 2021Quote: Bioluminescence signals in exosomes and MVs (3 × 107 EVs/µL) carrying PalmReNL were measured at room temperature (25°C) using a VICTOR Nivo Microplate Reader (PerkinElmer). EVs were incubated in the presence or absence of 1% Triton X-100 for 30 min at 37°C ...
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bioRxiv - Neuroscience 2021Quote: ... The signal of CB1 receptor hybridization was revealed by a TSA reaction using cyanine 3 (Cy3)-labeled tyramide (1:100 for 10 minutes, NEL744001KT PerkinElmer). After several washes ...
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bioRxiv - Cancer Biology 2020Quote: ... Mice were monitored every 3-4 days by non-invasive luciferase imaging by intraperitoneal injection of 150 mg/kg Luciferin (PerkinElmer) and quantification of luciferase activity using Imaging Systems (IVIS Spectrum System ...
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bioRxiv - Neuroscience 2021Quote: ... Detection was performed using the either the TSA Cyanine 3 Plus or the TSA Plus Fluorescein Evaluation Kits (Perkin Elmer).
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bioRxiv - Molecular Biology 2021Quote: ... from which aliquots of 10 μL were counted in vials with 3 mL OptiPhase HiSafe3 scintillation fluid (PerkinElmer, #1200-437). The fraction of charged tRNAAsn was calculated based on the charged tritium labeled aspartate incorporated into the pellet ...
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bioRxiv - Synthetic Biology 2021Quote: ... Live and dead cell counts were measured daily over a 3-day period using the Operetta High Content Screening system (PerkinElmer). Prior to imaging ...
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bioRxiv - Biochemistry 2021Quote: Structural models of polyenoyl tetramic acids 3 and 4 were built and energy minimized using Chem3D v.16.0 (Perkin Elmer). The terminal region of polyenes ...
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bioRxiv - Genomics 2021Quote: ... and transferred to a plastic scintillation vial containing 10 mL of OptPhase HiSafe 3 scintillation cocktail (PerkinElmer, Waltman, MA, USA). All vials were counted for 10min using a Tri-Carb 2810 TR liquid scintillation analyzer (PerkinElmer ...