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Citations for PerkinElmer :
351 - 400 of 920 citations for Mouse NLR Family CARD Domain Containing 4 NLRC4 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... 4 µL of RNA was 3’-end-labeled with 32pCp (prepared by incubating 16.5 µl of γ-32P-ATP (PerkinElmer), 1 µl of T4 polynucleotide Kinase ...
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bioRxiv - Molecular Biology 2022Quote: ... RNA samples were separated on a 7 M urea denaturing polyacrylamide gel at 4°C at 100V and transferred onto a charged nylon transfer membrane (PerkinElmer), followed by cross-linking using a UV Stratalinker and drying at 80°C on a Gel Dryer (Bio-Rad).
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Aerrane (Isoflurane, Baxter) inhalation was performed for anesthesia using the RAS-4 Rodent Anesthesia System (Perkin Elmer, Waltham, MA, USA) for experiments with IVIS Spectrum CT living imaging system (Perkin Elmer ...
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bioRxiv - Molecular Biology 2022Quote: ... was next added and incubated with the mixture at 4 °C for 1 hour before the measurements performed on Envision Readers (PerkinElmer). The fluorescently-labeled m6A-containing mRNA was used to adjust the gain factor ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were washed with PBS and then stored in PBS at 4°C until imaged in the Opera Phenix (PerkinElmer) at 40x magnification and images analysed using a Harmony software to assess protein expression and morphology (see supplementary).
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bioRxiv - Bioengineering 2024Quote: ... The plates were incubated for 4 h at 37°C and then the supernatant was removed and incubated with scintillation fluid (PerkinElmer) overnight at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... membranes were blocked with 5% BSA in Tris / HCl-buffered saline / 0.05% Tween (TBST) for 24 h at 4 °C and afterwards membranes were incubated for 1 h with HRP-Streptavidin (PerkinElmer; #NEL750001EA ...
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bioRxiv - Neuroscience 2020Quote: ... Mouse recPrP23-231 at 65 nM in PBS was coated to 384 well HB plates (Perkin Elmer), overnight at 4°C ...
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bioRxiv - Cancer Biology 2020Quote: ... and incubated for 10 min at room temperature with Opal Polymer HRP Mouse Plus Rabbit secondaries (PerkinElmer). After another wash in PBS ...
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bioRxiv - Cancer Biology 2020Quote: ... and incubated for 10 min at room temperature with Opal Polymer HRP Mouse Plus Rabbit secondaries (PerkinElmer). After another wash in PBS ...
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bioRxiv - Molecular Biology 2020Quote: ... HRP-conjugated secondary anti-rabbit and anti-mouse IgG antibodies were obtained from PerkinElmer (Waltham, MA, USA). Vectashield antifade mounting medium with 4’,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Microbiology 2021Quote: ... The washed filters were dried for 30 min under a heat-lamp and placed in a 20-ml Borosilicate Glass Scintillation vial containing 5 ml of Ultima Gold F liquid scintillation cocktail (Perkin Elmer, MA, USA). 14C radioactivity retained on the filters was quantified using an LS6500 scintillation system (Beckman Coulter Inc. ...
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bioRxiv - Biochemistry 2021Quote: ... alone or in the presence of 100 μM Na2S was assayed for its ability to autophosphorylate in a reaction containing 50 µCi of [γ-32P]-labeled ATP (6000 Ci/mmol, PerkinElmer Health Sciences), 100 mM Tris-HCl ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... After 6-24 h at 37 °C (as specified in each figure legend) the cell medium was aspirated and substituted with serum-free cell medium containing [3H]thymidine (2 μCi/ml, Perkin Elmer, Waltham, MA). The incubation was continued for 30 min at 37 °C ...
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bioRxiv - Cell Biology 2020Quote: In vitro kinase reactions were developed in a final volume of 50 μl containing 0.02 mM [γ-32P]ATP (1 µCi per tube, Perkin Elmer, Massachusetts, USA), 50 mM Tris-HCl ...
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bioRxiv - Neuroscience 2023Quote: ... Reactions were initiated by adding 2.5 µM ATP at 27°C and were terminated after 60 min with 10 µL of 2.5X Stop Solution/Detection Mix containing 20mM EDTA and 4nM LANCE Ultra Europium-anti-phospho-PKC (Ala25Ser) peptide antibody (Perkin Elmer, Waltham, MA). After incubation at 27°C for another 60 min ...
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bioRxiv - Cancer Biology 2024Quote: ... then separated in an 1% formaldehyde-containing agarose gel and transferred onto a GeneScreen Plus hybridization transfer membrane (Perkin Elmer, Waltham, MA, #NEF987001PK). RNAs on the membrane were crosslinked by exposing to UV light ...
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bioRxiv - Cancer Biology 2020Quote: ... amplified with RNAscope 2.5 HD Reagent Kit (Advanced Cell Diagnostics; 322300) and developed with the TSA Plus kit (PerkinElmer; NEL760001KT) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... and the Western Lightning ECL Pro kit (PerkinElmer). The signals were quantified with a ChemiDoc MP imaging system (Bio-Rad).
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bioRxiv - Pathology 2022Quote: ... amplified with TSA Cy3 kit (PerkinElmer Cat. FP1170), αSMC actin-Alexa Fluor 660 (Invitrogen Cat ...
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bioRxiv - Developmental Biology 2022Quote: ... the NEXTFLEX Rapid XP DNA-Seq kit (PerkinElmer) and xGen Lockdown probes (Integrated DNA Technologies ...
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bioRxiv - Neuroscience 2021Quote: NEXTflex small RNA-seq Kit v3 (Perkin Elmer) was used to ligate 5’ and 3’ adapters to purified RPF fragments ...
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bioRxiv - Molecular Biology 2023Quote: ... and DNA High Sensitivity Reagent Kit (CLS760672, PerkinElmer). Libraries with unique dual indexes were pooled and sequenced on partial lanes targeting ∼50M reads per sample on a HiSeq or a NovaSeq 6000 sequencer (Illumina ...
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bioRxiv - Cell Biology 2023Quote: ... using DNA High Sensitivity Reagent Kit (PerkinElmer: CLS760672) and LabChip GX Touch 24 (PerkinElmer ...
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bioRxiv - Immunology 2024Quote: DELFIA EuTDA Cytotoxicity Reagents kit (PerkinElmer, Waltham, MA) was used for ADCC assay as previously described (25 ...
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bioRxiv - Genetics 2023Quote: ... Nextflex Small RNA-Seq Kit v3 (Perkin Elmer) was used for all samples ...
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bioRxiv - Genetics 2023Quote: ... or NextFlex small RNA sequencing kit (Perkin Elmer) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... Plates were stored in the dark at 4°C prior to imaging using a 40x objective mounted on an Operetta High-Content Imaging System (Perkin Elmer) controlled by Harmony 4.1 software.
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bioRxiv - Cell Biology 2021Quote: ... diluted in buffer A to a final concentration of 4 nM) was added to a 384-well microplate (OptiPlateTM-384 Plus, Perkin Elmer) followed by the addition of 15 μL diluted proteins and 5 μL of the GFP antibody-conjugated acceptor beads (5x) ...
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bioRxiv - Biophysics 2020Quote: ... 100 nM hNatB was mixed with a saturating concentration of “MDVF” peptide substrate (500 μM) and varying concentrations (1.95 μM to 1 mM) of acetyl-CoA (14C-labeled, 4 mCi mmol−1; PerkinElmer Life Sciences) for 10-minute reactions ...
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bioRxiv - Plant Biology 2022Quote: ... 5-mm root segments away from the apical 2 mm root tip and 2 mm away from the root-shoot junction were carefully cut and soaked overnight in 4 ml of liquid scintillation fluid (Ultima Gold, PerkinElmer, USA), and the radioactivity was measured with a scintillation counter (model LS6500 ...
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bioRxiv - Physiology 2020Quote: ... Each filter was then placed in a Sterilin™ scintillation vial and radioactivity determined by: addition of 4 mL of Ultima Gold XR liquid scintillant (PerkinElmer), overnight incubation at room temperature and the retained radioactivity determined using a Beckman Coulter LS 6500 Multi-purpose scintillation counter (Beckman Coulter Inc. ...
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bioRxiv - Immunology 2019Quote: ... After 4-6 washes with 1x TBST the membrane was treated with chemiluminescent substrate Western Lightning ECL Pro (Perkin Elmer; NEL120001EA) according to manufacturer’s instructions ...
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Surveying the landscape of tRNA modifications by combining tRNA sequencing and RNA mass spectrometrybioRxiv - Biochemistry 2019Quote: ... at 42 °C for 30 min followed by hybridization overnight at 42 °C with 4 pmol DNA probes radiolabeled with [γ-32P]ATP (PerkinElmer) and T4 Polynucleotide kinase (New England Biolabs) ...
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bioRxiv - Plant Biology 2019Quote: ... 5 mm root segments away from the apical 2 mm were carefully cut and soaked for overnight in 4 mL liquid scintillation fluid (ULTIMA GOLD, PerkinElmer, USA), and the radioactivity was measured with a scintillation counter (model LS6500 ...
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bioRxiv - Microbiology 2022Quote: ... at 42 °C for 30 min followed by hybridization overnight at 42°C with 4 pmol probes (Table S3) that were radiolabeled using [γ-32P] ATP (PerkinElmer) and T4 Polynucleotide kinase (New England Biolabs) ...
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bioRxiv - Neuroscience 2023Quote: ... Thawed serum samples were diluted with 1xPBS 1:4 and assayed in triplicates using a fluorometer (PerkinElmer, VICTOR3V 1420 Multilabel counter) with excitation/emission of 485 nm / 535 nm (25 nm band-pass filter) ...
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bioRxiv - Molecular Biology 2023Quote: ... Transcription was initiated with addition of 12 pmol rrnBP1 PCR promoter fragment pre-mixed with 1 mM ATP and 4 μCi α-[32P]-CTP (3,000 Ci mmol−1; Perkin Elmer) at 37 °C ...
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bioRxiv - Neuroscience 2024Quote: ... and counted for 2 min after a 4 hr delay on a Perkin Elmer Betaplate 1205 liquid scintillation counter (Perkin Elmer). Nonspecific binding was determined with 1.0 µM dihydroergotamine.
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bioRxiv - Cancer Biology 2024Quote: ... 150 μL 14C-labelled lipids from the denser organic fraction were combined with 4 mL OptiPhase HiSafe 3 liquid scintillation cocktail (PerkinElmer; 1200.437) and radio-labeling was quantified using a Tri-Carb 2910 TR Liquid Scintillation Analyzer (PerkinElmer) ...
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bioRxiv - Physiology 2024Quote: ... media was changed to low-glucose DMEM supplemented with an additional 25 μM unlabelled phenylalanine and 4 μL of [14C]phenylalanine (NEC284E050UC; Perkin Elmer) per mL for 6 h ...
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bioRxiv - Molecular Biology 2023Quote: ... Fluorescently labeled RNA was obtained by replacing 1/4 volume of CTP with Cyanine 3-CTP (Cy3-CTP) or Cyanine 5-CTP (Cy5-CTP) (PerkinElmer NEL581001EA) in the in vitro transcription reaction.
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bioRxiv - Microbiology 2019Quote: ... Cells were resuspended in 0.1 mL PBS and the assay started by the addition of 0.1 mL of the transport mixture containing [3H]-proline (PerkinElmer’s NEN® Radiochemicals; 0.4 μCi) in the presence of different concentrations of the indicated drug ...
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bioRxiv - Developmental Biology 2021Quote: Mouse adult skeletons were revealed after micro-CT scanning using the Quantum GX2 micro-CT Imaging System (PerkinElmer) at the small animal preclinical imaging platform of the Medical Faculty of the University of Geneva ...
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bioRxiv - Physiology 2020Quote: ... Blots were then washed with TBST prior to incubation with HRP-linked anti-mouse secondary antibody (Perkin Elmer) for 1 h at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... SmFISH stained mouse spinal cord slides were imaged on an Operetta CLS high-content screening microscope (Perkin Elmer). Image acquisition was controlled using Perkin Elmer’s Harmony software ...
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bioRxiv - Microbiology 2019Quote: ... anti-rabbit or anti-mouse secondary antibodies conjugated to horseradish peroxidase were used at 1:10,00 dilution (PerkinElmer). Immunoblots were developed using PerkinElmer Western Lightning Plus-ECL and imaged with a GE Healthcare Amersham Imager 600 ...
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bioRxiv - Immunology 2020Quote: ... Staining of neutrophils (specifically myeloperoxidase activity) in LWT wild-type larvae was completed using TSA staining kit (Cy5-TSA Cyanine Kit, PerkinElmer, NEL705A001KT). Fish were incubated in a 1:100 ratio of Cy5-TSA:amplification diluent at 28°C for 10 minutes in the dark ...
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bioRxiv - Neuroscience 2019Quote: ... Confluent cultures were changed by quiescent medium for 24 h before they were labelled for 4 h with 1 μCi/ml [3H] arachidonic acid (PerkinElmer Life Sciences). Washing cells once with PBS containing 0.1% free fatty acid albumin and twice with PBS alone ...
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bioRxiv - Microbiology 2022Quote: ... were performed in a HORIBA JOBIN YVON Spectrofluorometer (FluoroMax – 4) (HORIBA Scientific, Edison, New Jersey, USA) in a fluorescence cuvette of path length 5 mm (Perkin Elmer B0631123), after putting 0.5ml of the sample solution in it ...