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Citations for PerkinElmer :
351 - 400 of 401 citations for IL 10 Mouse since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Dissociated cells were resuspended in 7.5 mg/mL BME-2 matrix gel at 2-5×104 cells/mL and seeded in 10 μL droplets to individual wells of 96-well Cell Carrier Ultra plates (Perkin Elmer). Once settled ...
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bioRxiv - Immunology 2023Quote: Vero E6 cells were grown in DMEM supplemented with 10% FBS and seeded into white-walled 96 well plates (PerkinElmer, 6005688) at a density of 20,000 cells per well ...
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bioRxiv - Molecular Biology 2024Quote: ... and 50 ng was used for random priming reactions in the presence of the radiolabeled nucleotide [α-32P]-dCTP (3,000 Ci/mmol, 10 mCi/ml, Perkin Elmer) using a random primer DNA labeling kit (TaKaRa ...
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bioRxiv - Molecular Biology 2023Quote: ... 50 ng of heat-denatured DNA was incubated for 30 min at 37°C in a total volume of 25 μl containing random primer and 5 μl of [α-32P]-dCTP (3,000 Ci/mmol, 10 mCi/ml; Perkin Elmer). After the reaction ...
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bioRxiv - Microbiology 2023Quote: ... The plate was incubated in the dark for 10-15 minutes before luminescence was read on a Victor3V 1420 Multilabel Counter (Perkin Elmer).
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bioRxiv - Molecular Biology 2023Quote: ... were cultured at 6000 cells per well in DMEM (+10% FBS, +1% P/S) on Perkin Elmer LLC CellCarrier-96 Ultra Microplates (Perkin Elmer) coated with PLO overnight ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were briefly vortexed and added to 20 mL liquid scintillation vials to which 10 mL of Ultima Gold was added (Perkin Elmer). The amount of glycosylated Rac1 was determined by liquid scintillation counting of each sample for 1 min using a Tri-carb 2910TR scintillation counter (Perkin Elmer) ...
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bioRxiv - Biochemistry 2023Quote: ... Reactions are incubated at 30 °C for 20 min prior to luminescence measurement for 10 s on the Victor X5 Multilabel Plate Reader (Perkin Elmer) by addition of 75 μl Renilla luciferase substrate (Promega) ...
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bioRxiv - Cancer Biology 2023Quote: ... Immuno-complexes were incubated for 20 min at 30°C in 30 μl of kinase buffer with a final concentration of 50 μM ATP and [γ-32P] ATP (1 x 10−2 μCi/pmol; PerkinElmer). Reactions were stopped by adding 5X LB ...
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bioRxiv - Biochemistry 2024Quote: ... For competition binding experiments cell membranes expressing h5-HT3A WT or mutant receptors were incubated in 250 µL 10 mM HEPES buffer (pH 7.4) containing 0.3 nM [3H]-GR65630 (Perkin Elmer, Waltham, MA) and varying concentrations of VTX or analogues ...
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bioRxiv - Molecular Biology 2024Quote: ... the remaining 20% of the beads (supplemented with 40 µl of 2X ROTI®Load and boiled at 95°C for 10 min) were subjected to SDS-PAGE for immunoblotting together with 1% input samples using HRP-streptavidin conjugate (PerkinElmer; #NEL750001EA) or anti-p65 (Santa Cruz Biotechnology ...
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bioRxiv - Neuroscience 2024Quote: ... washed one time with PBS and one time with PBS+DAPI (1:10 000) and subsequently imaged on the Opera Phenix High Content Screening System (Perkin Elmer) on a 40X water objective ...
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bioRxiv - Cell Biology 2024Quote: ... for 30 minutes before changing to MOPS-buffered ‘Air medium’ with 10% serum but lacking luciferin and moving to an Opera Phenix Plus (Revvity Perkin Elmer, at the UCSC Chemical Screening Center RRID SCR_021114) ...
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bioRxiv - Cancer Biology 2024Quote: ... and incubated for 10 minutes at room temperature before images were taken using IVIS Spectrum In Vivo Imaging System (Perkin Elmer). Wnt-activation signal was calculated as the bioluminescent signal captured by the IVIS Spectrum normalized to the tumor volume recorded per animal ...
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bioRxiv - Cancer Biology 2024Quote: ... From each well was transferred 10 µL of the lysates to a 384-well Optiplate™ (Perkin Elmer, catalog no. 6007290). To each well was added Acceptor Mix (5 µL ...
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bioRxiv - Biophysics 2023Quote: Laser surgery was performed on a Zeiss Axio Imager M2m microscope equipped with a Yokogawa CSU-10 spinning disk confocal head (Perkin Elmer), a Hamamatsu EM-CCD camera and a Zeiss 40X ...
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bioRxiv - Developmental Biology 2022Quote: ... anti-mouse Chd7 primary antibody was followed by signal amplification with goat anti-rabbit IgG horseradish peroxidase (1: 500) (Perkin Elmer Inc: NEF812001EA, Waltham, MA). The tyramide signal amplification plus Cy3 Kit (1:1500 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Animals were injected intravenously 24h after the last pharmacological treatment with Hypoxisense 680 (2 nmol/100 uL per mouse, Perkin Elmer, Life Sciences, Zaventem, Belgium), and the Hypoxisense 680 was measured in vivo 24h post-injection using the IVIS Spectrum (Perkin Elmer ...
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bioRxiv - Cancer Biology 2021Quote: ... the mice underwent BLI beginning at 10 days after implantation and every week thereafter with the In Vivo Imaging System (IVIS, PerkinElmer, Waltham, MA), and luminescence flux was recorded to assess tumor growth ...
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bioRxiv - Cell Biology 2020Quote: ... was added into the cells for incubation in the dark for 10 min prior to reading on an Enspire 2300 multilable reader (Perkin Elmer, USA). Measurements were done at least in triplicates and relative luciferase units (RLU ...
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bioRxiv - Cell Biology 2019Quote: ... Plates were left at room temperature for 10 min before imaging the ATP-coupled luminescence using a ViewLux microplate imager (PerkinElmer, Waltham, MA).
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bioRxiv - Cell Biology 2021Quote: RAW cells were incubated for 24 h with inositol-free DMEM (MP Biomedica, CA) containing 10 μCi/ml myo-[2-3H(N)] inositol (Perkin Elmer, MA), 1X insulin-transferrin-selenium-ethanolamine (Gibco) ...
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bioRxiv - Cancer Biology 2020Quote: Resulting cells were ultra-concentrated in 10 μl of DPBS and plated onto wells of a CellCarrier-96 Ultra Microplates (PerkinElmer, Cat# 6055302). Plates were then centrifuged at 1800g for 10 minutes to allow the cells stick to the surface of the wells ...
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bioRxiv - Microbiology 2022Quote: ... an appropriate concentration of DSF/BDSF/trans-BDSF was mixed with the RpfC liposomes and the resulting solution was incubated with 100 µM ATP containing 10 µCi [γ-32P]ATP (PerkinElmer, USA) in autophosphorylation buffer (50 mM Tris-HCl ...
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bioRxiv - Microbiology 2019Quote: ... Parasite numbers were correlated with bioluminescence intensity by plating a two-fold serial dilution of cells.10 Plates were read with an EnVision Multilabel Plate Reader (Perkin Elmer, USA) after incubation at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: ... Plates sat at room temperature for an additional 10 min in the dark before samples were read on an EnVision XCite plate reader (PerkinElmer # 2105-0020).
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bioRxiv - Microbiology 2019Quote: ... Nluc activity was measured from cell lysates within 10 min of lysis (or substrate addition) by transferring lysate into white OptiPlate 96-well plates (PerkinElmer, Waltham, MA) and measured in a Berthold Centro XS3 LB 960 luminescent plate reader with readings integrated over 0.2 sec.
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bioRxiv - Biochemistry 2021Quote: ... metabolic labeling was started by replacing the medium with phosphate-free DMEM containing 10% dialyzed FBS and 1mCi/mL 32P orthophosphate (PerkinElmer, Waltham, MA). After labeling for 8 hours ...
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bioRxiv - Cell Biology 2023Quote: ... Plates were shaking at 999 rpm and 28°C and growth was measured by monitoring OD600 every 2 h for 10 h using a plate reader (2300 EnSpire™, Perkin Elmer).
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bioRxiv - Physiology 2023Quote: ... was prepared by mixing 40 µL homogenate with an equal volume Nanoluc reaction buffer (PBS: NanoGlo Buffer: NanoGlo substrate furimazine, 30: 10: 0.2) in a black perkin elmer plate (6065400, PerkinElmer, Waltham, MA, USA). Within 2 min of buffer addition ...
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bioRxiv - Microbiology 2023Quote: ... The 3280 compounds were tested in a final concentration of 10 µM with the rhlA-gfp [30] reporter strain in 96 wells microtiter dish (Black Isoplate®, Perkin Elmer). The cells were added in a final concentration of OD600=0.1 and final volume of 200 µl in ABGC medium and incubated at 37°C with shaken (200 rpm) ...
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bioRxiv - Cancer Biology 2024Quote: ... Mice were injected IP with 10 µL/g of body weight of 15 mg/mL in vivo grade D-Luciferin (luciferin; PerkinElmer, Waltham, MA), anesthetized with 2-3% isoflurane in an induction chamber ...
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bioRxiv - Bioengineering 2021Quote: ... The volume of samples was adjusted to 10 mL by adding ultra-pure water and Gd concentration in each sample was measured using ICP-Mass Spectrometer (PerkinElmer, NexION® 2000). A similar procedure was used for monolayer cell culture (the cells were rinsed with PBS (3x 5 min ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 80-120 mg/kg body weight) / xylazine (Xy, 5-10 mg/kg body weight) cocktail) using an IVIS Spectrum In Vivo Imaging System (Perkin Elmer, Rodgau, Germany) with images acquired using the Living Image® software package (Perkin Elmer).
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bioRxiv - Plant Biology 2021Quote: ... Fluorescence spectra of solutions diluted 10-fold were recorded with 3.5 mL Suprasil® quartz optical cells of 10 mm pathlength using a LS-50B spectrofluorimeter monitored with UVWinlab 5.1 software (Perkin Elmer, Waltham USA). For each compound ...
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bioRxiv - Plant Biology 2020Quote: ... detection limit 5 ng.L−1) and K+ content by ICP-AES (-Atomic Emission Spectrometry, OPTIMA 8300, Perkin Elmer, quantification limit 10 μg.L−1).
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bioRxiv - Cancer Biology 2022Quote: ... Organoid/hydrogel cultures were serum starved overnight before treatment with EGF (20 ng/mL) and promegestone/R5020 (1, 10, 100 nM; Perkin Elmer, Cat. #: NLP00400) and 10 nM R5020 with or without the addition of FAK (1 μM ...
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bioRxiv - Plant Biology 2019Quote: ... 10mm root tip from 10 seedlings were collected for each sample and placed into the scintillation vial with 500µL MicroScint 40 (Perkin Elmer, Inc., USA, www.perkinelmer.com). The uptake of 137Cs+ was determined with a NaI scintillation counter (ARC-300 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... lung tissues harvested from euthanized mice were soaked for 10 min in pre-warmed (37°C) PBS with 2% FBS and a 1:10 dilution of Xenolight RediJect Coelenterazine h (Cat# 706506, Perkin Elmer, Waltham, MA). Following a 12-15 min incubation ...
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bioRxiv - Genomics 2020Quote: Monocytes from three unrelated healthy participants were plated in 30 µL of RPMI 1640 and 10% FBS on CellCarrier-384 well Ultra Microplates coated with PDL (PerkinElmer; cat. no. 6057500) at a concentration of 100,000 cells/well ...
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bioRxiv - Microbiology 2020Quote: ... 20 μCi of radioactive Pi solution (10 μL from a 2 mCi K2H32PO4 at a concentration of 2 mM of K2H32PO4, PerkinElmer cat. no. NEX055) was added to 1 mL of cell suspension ...
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bioRxiv - Genetics 2019Quote: ... evaporated under nitrogen gas (Organomation Associates, Inc., Berlin, MA, USA) and resuspended in 10 mL Insta-Gel Plus scintillation fluid (Perkin Elmer, Cat#6013399), followed by counting with a Beckman LS6500 scintillation counter ...
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bioRxiv - Cancer Biology 2023Quote: ... was injected intraperitoneally at a dose of 150 μg/g and incubated for 10 min before scanning in the IVIS® Spectrum In Vivo Imaging System (PerkinElmer, Waltham, MS).
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bioRxiv - Molecular Biology 2023Quote: ... The PCR tube was incubated at 80 °C for 10 minutes before cooling to 12 °C using a GeneAmp 2400 thermal cycler (Perkin Elmer, Waltham MA).
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bioRxiv - Genomics 2024Quote: ... size-selected sample was reamplified with 25 μL of NEXTFLEX PCR Master Mix 2.0 and 2 μL of 1:10 diluted NEXTFLEX Primer Mix 2.0 as described in the NEXTFLEX Cell free DNA-Seq Kit (V2) (Perkin Elmer, Pittsburgh, Pennsylvania, USA), step C PCR amplification ...
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bioRxiv - Cell Biology 2021Quote: ... hand-picked human islets were dispersed in accutase for 10 min at 37°C and plated in 96-well plates (Perkin Elmer Inc., Waltham, MA) treated with Poly-D-Lysine Hydrobromide (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2021Quote: ... and 10 μL of 1/5000 dilution of Eu-W8044 labeled streptavidin and Biotin-cAMP in the Detection Buffer (PerkinElmer Life and Analytical Sciences) were added in reduced lighting conditions ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and 10 μL of 1/5000 dilution of Eu-W8044 labeled streptavidin and Biotin-cAMP in the Detection Buffer (PerkinElmer Life and Analytical Sciences) were added in reduced lighting conditions ...
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bioRxiv - Biophysics 2020Quote: ... and SYBR Gold to the specified dye concentrations (0.03 to 60 μM) and analyzed the sample in a 10 × 2 mm2 cuvette (Perkin Elmer UV/VIS Spectroscopy Cell B0631122) for 5 min for each condition ...
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bioRxiv - Molecular Biology 2019Quote: ... 0.375 μL of 0.1 M DTT and 1 μL of UTP (0.9 μL of 3 μM cold UTP, plus 0.1 μL of 3 μM [α-33P] UTP, Perkin Elmer, 3000 Ci /mmol, 10 μCi/μL), at a final volume of 18.75 μL ...