Labshake search
Citations for PerkinElmer :
351 - 400 of 500 citations for BN80933 CAS 214348 10 4 98% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2024Quote: ... increasing concentrations of carbachol (from 0.01 µM up to 10 µM) were used in presence of 200 pM [3H]N-Methylscopolamine ([3H]NMS; 78.9 Ci/mmol; PerkinElmer).
-
bioRxiv - Microbiology 2023Quote: ... Reactions contained 10 μL lysate with 250 ng of pcDNA T7 expression plasmid and 0.5 μL [35S] methionine/cysteine (PerkinElmer). Reactions were incubated at 30°C for 40 minutes chasing with 2 μL of 50 mg / mL unlabelled methionine/cysteine ...
-
bioRxiv - Microbiology 2022Quote: ... Reactions contained 10 μL rabbit reticulocyte lysate with 500 ng of pcDNA T7 expression plasmid and 0.5 μL [35S] methionine (PerkinElmer). Reactions were incubated at 30°C for 40 minutes before being chased with 2 μl of 50 mg / mL unlabelled methionine ...
-
bioRxiv - Biochemistry 2023Quote: ... The mice were anesthetized in a ventilated anesthesia chamber with 1.5% isofluorane in oxygen and imaged 10 min after the injection with an in vivo imaging system (IVIS, PerkinElmer). Luminescence was quantified using the Living Image software (PerkinElmer) ...
-
bioRxiv - Cell Biology 2023Quote: ... quantitative microscopy was performed using the Operetta HCS imaging system with a 10×/0.3 NA objective (PerkinElmer, Courtaboeuf, France). Images were analyzed using scripts developed in Acapella software (PerkinElmer ...
-
bioRxiv - Biochemistry 2023Quote: ... 10 μM purified IKK2 protein was first autophosphorylated in the presence of 100-200 nM of γ32P-ATP (PerkinElmer) in kinase assay buffer for 1 hour at 30°C ...
-
bioRxiv - Molecular Biology 2024Quote: ... Engraftment was first assessed about 10 days after inoculation using an IVIS SpectrumCT Preclinical In Vivo Imaging System (PerkinElmer) for whole body luminescence imaging under isoflurane anaesthesia ...
-
bioRxiv - Microbiology 2024Quote: Overnight cultures were diluted to an OD600 of ∼0.05 in 10 mL LB Miller broth supplemented with 5 µCi/mL 32P ortho-phosphoric acid (PerkinElmer) and grown until reaching an OD600 of ∼0.6 ...
-
bioRxiv - Plant Biology 2020Quote: ... Relative quantification of transcript levels was performed using the “Delta-delta CT method” as presented by PE Applied Biosystems (Perkin Elmer, Foster City, CA, USA). Data were normalized to the cysG transcript and data are shown in relation to E ...
-
bioRxiv - Neuroscience 2023Quote: ... The experiments were initiated by placing the choroid plexus in HEPES-aCSF containing 1 μCi/ml 86Rb+ (022-105721-00321-0001, POLATOM, as a tracer for K+ transport) and 4 μCi/ml 3H-mannitol (Perkin Elmer, as an extracellular marker [26]) at 37°C for 2 min in the absence or presence of 2 mM ouabain (O3125 ...
-
bioRxiv - Evolutionary Biology 2020Quote: Bioluminescence was measured in 100 μL culture (or a 10-fold dilution series) in an Envision Multilabel Plate Reader (PerkinElmer). Clonal bioluminescence was also qualitatively assessed by examining colonies on LB agar in an AlphaImager HP light box (ProteinSimple ...
-
bioRxiv - Immunology 2021Quote: ... The luciferase signal was determined in live animals one day after virus injection and 10 minutes after intraperitoneal injection of 150 mg/kg D-luciferin (PerkinElmer) using the in vivo imaging system NightOWL II LB 983 (Berthold ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... The plates were read every 10 minutes on a fluorescence microplate reader (EnVision 2104 Multilabel Reader (Perkin Elmer, MA, USA) at an excitation wavelength of 440 nm with an emission wavelength of 484 nm ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... The plates were read every 10 minutes on a fluorescence microplate reader (EnVision 2104 Multilabel Reader (Perkin Elmer, MA, USA) at an excitation wavelength of 440nm with an emission wavelength of 484nm.
-
bioRxiv - Pharmacology and Toxicology 2020Quote: Spectra were analysed using OPUS 6.5 software (Bruker) and compared with RMIT University’s spectral library of organic compounds generated using Spectrum 10 software (Perkin Elmer). Fingerprint spectra shown in Figure 4D were prepared using GraphPad Prism.
-
bioRxiv - Biochemistry 2021Quote: ... The samples were boiled at 95°C for 10 min and subjected directly to LabChip GX II capillary gel electrophoresis (PerkinElmer) according to the manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2021Quote: ... and 640 μM) were added along with 10 μM of non-radiolabeled UTP plus 6 mCi of [α32P]-UTP (PerkinElmer). Upon addition of nucleotides ...
-
bioRxiv - Cell Biology 2021Quote: ... 10mM MgCl2 and 1 mM MnCl2) supplemented with 10 μM ATP and 2 μCi γ-32P-ATP (blu002z250uc; Perkin Elmer) in 15μl reactions ...
-
bioRxiv - Microbiology 2022Quote: ... was phosphorylated in vitro with His-MBP-PknBMtb at 0.015 μg/μl for 30 minutes at room temperature with 0.15μCi/μl ATP [γ-32P] (5×10-8 μmol) (3000Ci/mmol, 10mCi/ml, PerkinElmer BLU002A250UC) and 2mM MnCl2 in buffer (50mM Tris ...
-
bioRxiv - Cancer Biology 2022Quote: ... luciferin was added to each well (10% of total volume per well) and plates were imaged using the IVIS Spectrum In Vivo Imaging System (PerkinElmer). Cell viability was measured as total photon flux per second ...
-
bioRxiv - Neuroscience 2021Quote: ... during 2 hours at RT followed by a 10 minutes incubation at RT with TSA plus Biotin System (Biotin TSA 1:250, NEL749A001KT PerkinElmer). After several washes ...
-
bioRxiv - Neuroscience 2021Quote: ... during 2 hours at RT followed by a 10 minutes incubation at RT with TSA Biotin System (Biotin TSA 1:100, NEL700A001KT PerkinElmer). After several washes ...
-
bioRxiv - Neuroscience 2021Quote: ... The signal of CB1 receptor hybridization was revealed by a TSA reaction using cyanine 3 (Cy3)-labeled tyramide (1:100 for 10 minutes, NEL744001KT PerkinElmer). After several washes ...
-
bioRxiv - Molecular Biology 2021Quote: ... from which aliquots of 10 μL were counted in vials with 3 mL OptiPhase HiSafe3 scintillation fluid (PerkinElmer, #1200-437). The fraction of charged tRNAAsn was calculated based on the charged tritium labeled aspartate incorporated into the pellet ...
-
bioRxiv - Biochemistry 2021Quote: ... 25 mM GTP and 0.25 mM CTP) and 0.5 µl of α-32P-CTP (3000 Ci/mmol, 10 mCi/ml Perkin Elmer) were supplemented into each reaction along with 1 ng of DNA template and 100 ng of competitor DNA ...
-
bioRxiv - Physiology 2020Quote: ... sections were incubated with an HRP-conjugated secondary antibody at an optimised dilution in NGS for 30 min at room temperature followed by a 10 min incubation with Tyramide solution (PerkinElmer). Primary and secondary antibodies and associated working dilutions are given in Supplementary Table 1 ...
-
bioRxiv - Genomics 2021Quote: ... and transferred to a plastic scintillation vial containing 10 mL of OptPhase HiSafe 3 scintillation cocktail (PerkinElmer, Waltman, MA, USA). All vials were counted for 10min using a Tri-Carb 2810 TR liquid scintillation analyzer (PerkinElmer ...
-
bioRxiv - Cancer Biology 2022Quote: ... 15 or 10-20% SDS-PAGE gels and proteins were transferred onto PVDF membranes (0.45 μm, Perkin Elmer, Waltham, MA) and blocked with 5% BSA/TBS-T for 2 hours at room temperature ...
-
bioRxiv - Molecular Biology 2023Quote: ... and 10 μL of 12 nM biotinylated nucleosome (or its mutants) were mixed and incubated in OptiPlate-384 microplate (PerkinElmer) for 60 mins ...
-
bioRxiv - Biochemistry 2024Quote: ... were transferred to 1.5 mL Eppendorf tubes containing 10 μL of pre-incubated at 37°C respective radioisotope (3H methionine [0.77 μCi, Perkin Elmer] ...
-
bioRxiv - Biochemistry 2024Quote: Influx and efflux assays were initiated after mixing 20 μl of PLs solution with 180 μl of influx transport buffer (10 μM L-serine in Tris buffer with and 0.5 µCi of L-[3H]-serine (Perkin Elmer) or 4 mM cold amino acid for efflux experiments ...
-
bioRxiv - Cell Biology 2024Quote: Plasma samples were collected in two 10-ml EDTA and a 4.5-ml tube with sodium citrate and centrifuged at 2,000×g for 10 min as previously described (Durat et al, 2014) and fractionated by automation (Janus robotic liquid handlers; PerkinElmer). Typically ...
-
bioRxiv - Cancer Biology 2024Quote: Images of the drug-treated samples (10-17 images per well) were acquired using Opera Phenix High Content Screening System (PerkinElmer) with 40x objective and Andor Zyla sCMOS camera with Harmony High Content Imaging and Analysis Software (PerkinElmer) ...
-
bioRxiv - Neuroscience 2023Quote: ... and imaged 10 minutes after D-luciferin injection with a cooled charge-coupled device (CCD) camera (In Vivo Imaging System by PerkinElmer). Mice were imaged regularly every week until week 8 and every 4 weeks until week 28 ...
-
bioRxiv - Genetics 2023Quote: ... 10 µg of total RNA was polyA-selected and processed using the NEXTFLEX Rapid Direction RNA-seq Kit (NOVA-5138-10 and NOVA-5138-11; PerkinElmer) according to the provided manual ...
-
bioRxiv - Cancer Biology 2023Quote: ... mice were given 10 μL/g of D-luciferin (15 mg/mL) (Gold Bio) intraperitoneally and imaged using the IVIS Spectrum imager (PerkinElmer). Luciferase signal was quantified 15 min after injection ...
-
bioRxiv - Systems Biology 2023Quote: ... A breathable membrane was applied over the plate and cells were imaged every 10 min on the Operetta CLS high-content microscope (PerkinElmer) at 37°C and 5% CO2 for 72h using the 20x N.A ...
-
bioRxiv - Biochemistry 2023Quote: ... was mixed in 20 µl of the cytoplasmic buffer with 2- to 10-fold excess of recombinant Sm proteins and each sample was supplemented with 2 µCi of 3H-labeled SAM (PerkinElmer) for overnight methylation at 32 °C ...
-
bioRxiv - Cancer Biology 2023Quote: ... and tissues were rapidly imaged (within 10 minutes post-sacrifice) with the bioluminescence signal being assessed with the IVIS Spectrum In Vivo Imaging System (PerkinElmer). Tissue tumor burden was calculated based on the total flux (photons per second [p/s] ...
-
bioRxiv - Microbiology 2023Quote: ... Plates were read once every 30 s for 10 min at 37°C using a EnSpire Multimode Plate Reader (PerkinElmer) with excitation and emission wavelengths of 550 and 591 nm ...
-
bioRxiv - Microbiology 2023Quote: ... purified 6×His-tagged SfTIR-STING was added to the reaction to a final assay volume of 50 μL and plates were read once every 15 s for 10 min at 37°C using a EnSpire Multimode Plate Reader (PerkinElmer) with excitation and emission wavelengths of 410 and 300 nm ...
-
bioRxiv - Molecular Biology 2023Quote: ... was done using the same procedure for the cold transcription except for the use of 0.5 µl alpha-32P-labeled UTP (3000 Ci/mmol-10 mCi/ml) (Perkin Elmer) and 0.8 mM UTP (instead of 2 mM) ...
-
bioRxiv - Molecular Biology 2024Quote: ... Detection reagent was then added and incubated for 10 min at RT prior to luminescence detection using a MicroBeta2 microplate counter (PerkinElmer). Experiments were performed as biological triplicates and results were plotted using GrapPad Prism ...
-
bioRxiv - Neuroscience 2024Quote: ... and transfected cells were plated evenly across 10 wells of poly-D-lysine-coated 96-well PhenoPlates (PerkinElmer; Cat# 6055508). Half-media changes of BMDM medium containing 50ng/mL M-CSF were performed every other day and endpoints were assessed until 14 days after transfection.
-
bioRxiv - Biochemistry 2024Quote: ... 10 µg RNA samples were separated on 2% glyoxal agarose gels and transferred onto nylon membranes (GeneScreen Plus, Perkin Elmer). Complementary [32P]-labeled probes were synthesized by linear PCR and hybridized at 65°C overnight in hybridization solution (0.5 M NaP pH = 7.0 ...
-
bioRxiv - Biochemistry 2024Quote: ... then incubated with Ham’s F-10 media supplemented with 1.5 mCi/well myo[2-3H(N)] inositol (Perkin Elmer/Revvity) for 16-18 h ...
-
bioRxiv - Cell Biology 2024Quote: ... for 10 min before being imaged with an Ultra View VOX confocal laser scanning microscope (Perkin Elmer, Waltham, MA, USA).
-
bioRxiv - Cancer Biology 2024Quote: ... across 25 x 25 fields of view with a 10% overlap were acquired per well on an Opera Phenix High Content Screening System (PerkinElmer) using a 40x or 63x/1.15 NA water-immersion objective ...
-
bioRxiv - Cell Biology 2024Quote: ... 10 µg/mL of SYNs were added to hMG plated on a CellCarrier™-96 Ultra Microplate (Perkin Elmer®) and imaged using 20x objective of a Zeiss SP05 confocal microscopy ...
-
bioRxiv - Cell Biology 2021Quote: ... Cells were imaged at 1 frame/30 s for 4 h at 37°C with 5% CO2 using a spinning disk confocal microscope (UltraVIEW VoX, PerkinElmer with a 63X 1.4 N.A. oil objective), followed by addition of 50 μg/ml PI (Thermo Fisher Scientific ...