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Citations for PerkinElmer :
301 - 319 of 319 citations for Recombinant Human LDLR protein GST tagged since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Horseradish peroxidase (HRP) conjugated secondary antibody was added in order for labelled proteins to be detected by Western Lightning Plus-ECL (PerkinElmer, 203-170071). Primary and secondary antibodies used are listed in Supplementary Table 5.
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bioRxiv - Cell Biology 2022Quote: ... HEK293T cells transfected with FTTTRA25T and were labeled for 30 min with EasyTag Express [35S] Protein Labeling Mix (0.1 mCi/mL; Perkin Elmer CAT# NEG772014MC) in DMEM lacking both Cys and Met (Gibco CAT# 21013024 ...
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bioRxiv - Molecular Biology 2022Quote: ... Membranes were finally washed three times with PBST for 5 min and protein levels were visualized by enhanced chemiluminescence (Perkin Elmer, NEL104001EA) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... Membranes were washed at room temperature 3 times 10 min in PBST before using chemiluminescence to reveal protein bands with the Western Lightning® Plus-ECL Enhanced Chemiluminescence reagent (PerkinElmer Inc.) as per the manufacturer’s instructions.
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bioRxiv - Biophysics 2020Quote: ... HEK cell membrane proteins (100 μg/ml final concentration) were incubated with 3 nM [3H]muscimol (30 Ci/mmol; PerkinElmer Life Sciences), neurosteroid (3 nM–30 μM ...
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bioRxiv - Molecular Biology 2024Quote: ... 6 ng/ µl recombinant TopBP1 protein or buffer were added to TopBP1 immunodepleted interphase Xenopus egg extract containing 50 nCi/µl α32P-dCTP (Perkin Elmer NEG513A250UC). To start the replication reaction the egg extracts were supplemented with 5 ng sperm DNA (kindly provided by the lab of O ...
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bioRxiv - Cell Biology 2024Quote: Confluent 6-well dishes of FRT cells (approximately 1×106/well) were metabolically labeled in DMEM depleted of methionine and cysteine and supplemented with EasyTag 35S Protein Labeling Mix (Perkin Elmer, NEG772007MC), glutamine ...
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bioRxiv - Immunology 2024Quote: ... Cells were then starved in Met/Cys-free medium at 37°C for 30 minutes with dialyzed FBS before replacing the medium with 35S-Cys/Met enriched medium (11 µCi/µL, EasyTag™ EXPRESS35S Protein Labeling Mix, Perkin Elmer) for 5 hours at 37°C ...
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bioRxiv - Molecular Biology 2024Quote: ... and 2.3±0.64 pmol/mg protein (Bmax for β 2AR) and binding affinities for the radioligand [³H]CGP12,177 (specific activity 51 Ci/mmol, PerkinElmer, Rodgau, Germany) of 0.070 nM (KD for β1AR ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: Biotinylated human recombinant KRAS WT, G12C, G12D, and G12V proteins (Amid Bioscience, Santa Clara, CA) were pretreated with Terbium-labeled streptavidin (Tb-SA) (PerkinElmer, Waltham, MA) and BODIPY-FL-GDP (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2024Quote: Capillary electrophoresis sodium dodecyl sulfate (CE-SDS) was performed on a LabChip GX II instrument using Protein Express 200 (Perkin Elmer, #760499) and Protein Express Assay Reagent Kit (Perkin Elmer ...
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bioRxiv - Cancer Biology 2024Quote: ... Equal amounts of cell lysate proteins (20-50 μg) or proteins eluted from agarose beads were separated on SDS-PAGE and transferred to polyvinylidene difluoride (PVDF) membranes (PerkinElmer Life Sciences). Membranes were blocked 1 h with 3% BSA/TBST and incubated with primary antibodies overnight at 4°C ...
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bioRxiv - Cancer Biology 2020Quote: ... containing 10% BSA and 3 µl [35S] EasyTag Express protein labelling mix (7 mCi, 259 MBq, 1175 Ci/mmol in 632 ml; Perkin Elmer, Espoo, Finland). After 2 h of incubation at 37 °C cells were washed twice with PBS ...
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bioRxiv - Biochemistry 2023Quote: ... Ten μl of these dilutions or 10 μl of G protein storage buffer without nucleotides were pipetted into the corner of a white opaque 96-well plate (Opti-Plate, PerkinElmer Life Sciences, 6005290). Ten 10 μl of 0.1 mM Coelenterezine 400a diluted in BRET buffer (140 mM NaCl ...
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bioRxiv - Immunology 2021Quote: ... cells were pulse labelled with 50µCi of 35S-methionine and -cysteine for 1 min (EasyTag™ EXPRESS35S Protein Labeling Mix, Perkin Elmer, Waltham MA, USA) and immediately transferred into 10x volume of chase medium containing excess amounts of unlabelled cysteine (500 µg/ml) ...
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bioRxiv - Bioengineering 2023Quote: ... for SARS-CoV-2 S protein neutralization antibody titer in the wells was observed using the High Content Analysis System (Perkin Elmer, Waltham, MA, USA) or microscope ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: The level of phosphorylated extracellular signal-regulated protein kinase 1/2 (pERK1/2) was detected using the AlphaScreenTM SureFire Kit (PerkinElmer Life and Analytical Sciences). Briefly ...
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bioRxiv - Physiology 2023Quote: ... the medium was exchanged for DMEM medium supplemented with emetine and 35S-Protein Labelling Mix (35S-Met and 35S-Cys, Perkin Elmer NEG072; 100 µCi/mL). Cells were incubated for 3 h at 37 °C ...
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bioRxiv - Molecular Biology 2024Quote: DBS were acquired from consenting healthy volunteers via a finger prick onto blood sampling papers (ADVANTEC PKU-S; ADVANTEC; Whatman 903 Protein Saver Cards, Whatman; PerkinElmer 226 Spot Saver Card; PerkinElmer) and dried overnight.