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Citations for PerkinElmer :
301 - 350 of 365 citations for Phospholipase A and Acyltransferase 4 RARRES3 PLAAT4 Antibody Biotin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... Antigen-antibody complexes were detected with West Pico Chemi luminescent substrate using standard manufactures protocol (Perkin Elmer). Imaging of all western blots was performed using an UVP BioImager 600 system equipped with VisionWorks Life Science software (UVP ...
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bioRxiv - Molecular Biology 2020Quote: ... HRP-conjugated secondary anti-rabbit and anti-mouse IgG antibodies were obtained from PerkinElmer (Waltham, MA, USA). Vectashield antifade mounting medium with 4’,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Cancer Biology 2021Quote: ... followed by primary antibody and corresponding secondary horseradish peroxidase-conjugated polymer (Zytomed Systems, Germany or Perkin Elmer). Each horseradish peroxidase-conjugated polymer mediated the covalent binding of different fluorophore using tyramide signal amplification ...
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bioRxiv - Physiology 2024Quote: ... fluorescein was detected with an anti-fluorescein antibody conjugated to horseradish peroxidase (RRID: AB_2737388; PerkinElmer, Waltham, MA) and visualized using the TSA Plus Fluorescein System (PerkinElmer) ...
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bioRxiv - Molecular Biology 2023Quote: ... Following the incubation with 2ry HRP coupled antibodies western lightening ECL Pro ECL (Perkin Elmer, Massachusetts, USA) was added ...
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bioRxiv - Neuroscience 2023Quote: ... the binding sites of the secondary antibodies were detected using Western Lightning ECL Pro (PerkinElmer, MA, USA). Chemiluminescence was visualized using the Fusion Solo S imaging system (Vilber Bio Imaging ...
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bioRxiv - Neuroscience 2023Quote: ... Fluorescein was detected with an anti-fluorescein antibody conjugated to horseradish peroxidase (RRID: AB_2737388; PerkinElmer, Waltham, MA) and visualized using the TSA Plus Fluorescein System (PerkinElmer) ...
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bioRxiv - Biochemistry 2024Quote: ... The pellets were washed with PBS pH 7.4 + 0.1% BSA and then incubated with anti-FLAG antibody conjugated to allophycocyanin (anti-FLAG:APC; PerkinElmer) (diluted 1:100 into PBS pH 7.4 + 0.1% BSA ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were imaged at 1 frame/30 s for 4 h at 37°C with 5% CO2 using a spinning disk confocal microscope (UltraVIEW VoX, PerkinElmer with a 63X 1.4 N.A. oil objective), followed by addition of 50 μg/ml PI (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2020Quote: ... Blots were then washed with TBST prior to incubation with HRP-linked anti-mouse secondary antibody (Perkin Elmer) for 1 h at room temperature ...
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bioRxiv - Immunology 2020Quote: ... The integrated signal density corresponding to labeled antigen or antibody in each LN was calculated using LivingImage (PerkinElmer) or ImageJ software and plotted using GraphPad Prism 8 ...
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bioRxiv - Cell Biology 2021Quote: ... HRP-conjugated secondary antibody to adiponectin (Table S1) was visualized with Western Lightning Plus (Perkin Elmer, Waltham, Massachusetts) and imaged using a LI-COR Odyssey Imager (LI-COR Biosciences ...
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bioRxiv - Immunology 2023Quote: ... After secondary antibody incubation cells were directly imaged and analyzed using Operetta CLS High Content Analysis System (PerkinElmer).
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bioRxiv - Biochemistry 2024Quote: ... Secondary anti-mouse HRP antibody listed in the key resources table and Western Lightning Plus-ECL (PerkinElmer NEL105001) was used to visualize bands.
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bioRxiv - Cancer Biology 2020Quote: ... overnight and detected using HRP-conjugated secondary antibodies and chemiluminescence with the Western Lightning Plus-ECL Reagent (Perkin Elmer).
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bioRxiv - Neuroscience 2020Quote: ... HRP conjugated secondary antibodies were used for ECL-mediated visualization (Western Lightening Plus-ECL, #NEL105001EA, Perkin Elmer, Waltham, MA) with film (GeneMate Blue Lite Autorad Film ...
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bioRxiv - Immunology 2022Quote: ... Antibody binding was detected using enhanced chemiluminescent substrates for horseradish peroxidase (HRP) (ECL Western blotting reagents (PerkinElmer, cat: NEL105001EA) or SuperSignal™ West Femto Maximum Sensitivity Substrate (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... Slides were washed again and incubated with the appropriate Opal tertiary antibody (1:100 in amplification diluent, Perkin Elmer) for 60 min ...
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bioRxiv - Developmental Biology 2020Quote: ... incubated 1h in blocking buffer and then incubated overnight with an anti-DNP-POD antibody (1:1000, Perkin Elmer). The next day embryos were washed in PBS-Tween ...
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bioRxiv - Immunology 2024Quote: ... Antibody binding was detected using enhanced chemiluminescent substrates for horseradish peroxidase (HRP) (ECL Western blotting reagents (PerkinElmer, cat: NEL105001EA) or SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... 10 nM full-length 6x-His-tagged human G3BP1 produced in mammalian cells was added to the reaction mix containing test compound and 0.5 nM anti-His-Tb antibody (PerkinElmer). Assays were initiated by adding 4 nM FAM-labeled PEG6-USP10 24-mer peptide probe (FAM-PEG6-GALHSPQYIFGDFSPDEFNQFFVT ...
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bioRxiv - Developmental Biology 2023Quote: ... was used to detect the anti-pH3 antibody after detection of ISH probe by using TSA plus (Perkin Elmer) green (FP1168).
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bioRxiv - Immunology 2024Quote: ... or HIS-SUMO-C-A mutant Rb 380-785 was pre-incubated with 0.4 nM HTRF donor conjugated antibodies anti-GST-Tb or anti-HIS-Tb (PerkinElmer), respectively ...
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bioRxiv - Neuroscience 2024Quote: ... HRP-conjugated secondary antibodies were used for signal detection by the Western lightning chemiluminescence reagent (PerkinElmer, Waltham, MA, USA) and exposure to X-ray films.
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bioRxiv - Immunology 2021Quote: ... Immunoreactive proteins were detected with either anti-mouse or anti-rabbit horseradish peroxidase-coupled secondary antibody followed by detection with enhanced chemiluminescence (PerkinElmer).
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bioRxiv - Neuroscience 2021Quote: ... Molecular weight bands were visualized by incubation with peroxidase-conjugated anti-mouse or anti-rabbit secondary antibody (1:5000; Jackson Immuno Research Laboratories) followed by treatment with the ECL-plus reagent (Western Lighting; PerkinElmer) and exposure on G:Box Chemi XRQ system (Syngene) ...
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bioRxiv - Neuroscience 2021Quote: ... and Lumi4-Tb cryptate conjugated anti-IP1 antibody (emission at 620 nm) was detected using an Envision plate reader (PerkinElmer). Results were calculated from the 665/620 nm ratio and normalised to the maximum response stimulated by acetylcholine.
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bioRxiv - Neuroscience 2022Quote: ... membranes and immunoblots were carried out using primary antibodies (Table S4) followed by incubation with appropriate secondary horseradish peroxidase-conjugated antibodies (Table S5) and chemoluminiscent detection (Western Lightning, PerkinElmer). All antibodies were diluted in PBS containing 5% semi-skimmed milk and 0.1% Tween-20 ...
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bioRxiv - Neuroscience 2022Quote: ... membranes were washed and incubated with the goat anti-rabbit IgG HRP-labeled secondary antibody (PerkinElmer, Waltham, MA, USA; NEF812001EA) for 1 h at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... anti-mouse or anti-rabbit secondary antibody (1:10000; Jackson Immuno Research Laboratories) followed by treatment with ECL-plus reagent (Western Lighting, PerkinElmer) and exposure to autoradiography films ...
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bioRxiv - Developmental Biology 2021Quote: ... Antisense RNA probes labeled with DNP were used in all in situ hybridizations and probe detection was performed using a HRP-coupled anti-DNP antibody (PerkinElmer) and a fluorescein coupled tyramide (Vize et al. ...
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bioRxiv - Microbiology 2022Quote: ... The membranes were then incubated with anti-IgG rabbit antibodies conjugated to horseradish peroxidase (PerkinElmer Life Sciences (Boston, MA, USA), and developed using the Western Lightning Chemiluminescence Reagent Plus (PerkinElmer) ...
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bioRxiv - Neuroscience 2022Quote: ... then incubated for 30 min at 25 °C with shaking at 400 RPM with 9 ng/well of DELFIA Eu-N1 anti-mouse antibody in DELFIA assay buffer (PerkinElmer), washed with 3 × 100 μl of PBST before enhancing with 80 μl of DELFIA Enhancement Solution (PerkinElmer) ...
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bioRxiv - Biochemistry 2022Quote: ... 5 μL time points were taken and quenched with 1.33 mM EDTA then brought to 20 μL final volume for detection using 1 nM LANCE Ultra Europium anti-H3K4me1/2 antibody (TRF0402, PerkinElmer) and 50 nM LANCE Ultra Ulight-Streptavidin (TRF0102 ...
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bioRxiv - Immunology 2022Quote: ... Virus–monoclonal antibody-containing medium was then aspirated from cells and 100Lµl of a 1:2 dilution of SteadyLite Plus (PerkinElmer, 6066759) in PBS with Ca2+ and Mg2+ was added to cells ...
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bioRxiv - Microbiology 2024Quote: ... Blots were probed with the indicated antibodies and developed using the Western Lighting plus ECL (Perkin Elmer, Waltham, MA, USA). Blots were imaged on the Bio-Rad ChemiDoc MP Imaging System (BioRad ...
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bioRxiv - Biochemistry 2023Quote: ... FLAG and HA were detected using the LI-COR system and HRP-conjugated GAPDH primary antibody was incubated with blots for 2 hours at room temperature and detected with Western Lightning Plus-ECL (PerkinElmer) using a Bio-Rad Chemidoc System.
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bioRxiv - Developmental Biology 2024Quote: ... followed by secondary antibody incubation for 30 min at room temperature with HRP conjugated Goat anti Rabbit (Perkin Elmer, NEF812001EA). The slides were then developed with Opal 780 fluor at 1:25 (Akoya ...
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bioRxiv - Cell Biology 2020Quote: ... involving the use of a biotinylated secondary antibody followed by an ABC complex and Cy3-tyramide kit (Perkin Elmer, cat#: FP1046) or an Alexa secondary antibody (1:500 ...
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bioRxiv - Immunology 2021Quote: ... Cells were immunostained with DAPI and Alexa488-conjugated anti-N antibody (CR3009; produced in-house) and imaged using an Opera Phenix (Perkin Elmer). The ratio of infected (Alexa488+ ...
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bioRxiv - Bioengineering 2020Quote: ... Images are acquired by confocal microscopy to minimize auto-fluorescence of unlabeled dye/antibody, using a conventional laser scanning confocal microscope (CLSM510Meta, Zeiss) or a high-content screening system (Opera Phenix, Perkin Elmer). Instrument specifications are found in Supplemental Information ...
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bioRxiv - Cancer Biology 2020Quote: ... membranes were washed and probed with horseradish peroxidase (HRP)-conjugated secondary antibodies for 1 hour before detection with Western Lightning Chemiluminescence (Perkin Elmer). Antibodies used included ...
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bioRxiv - Cancer Biology 2021Quote: ... was used for antibody raised in rabbit (PH3 and PHGDH) and the OPAL Polymer HRP Ms+Rb (Akoya/Perkin Elmer, ARH1001EA) was used for the antibody raised in mouse (panCK) ...
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bioRxiv - Genomics 2021Quote: ... the anti-DIG-HRP antibody was detected with the TSA-Cy3 substrate (1:50, TSATM PLUS Cy3 kit, NEL 745001KT, Perkin Elmer) for 10 min at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... The membrane was probed with a goat anti-mouse antibody conjugated to horseradish peroxidase and bands were visualized using the Western Lighting Plus-ECL reagent (Perkin Elmer). When performing the KatG expression analysis in response to C10 treatment ...
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bioRxiv - Microbiology 2022Quote: ... and serum IgA antibodies to the secreted form of S-2P were measured by europium ion-enhanced DELFIA-TRF assay (Perkin Elmer) following the supplier’s protocol.
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bioRxiv - Cell Biology 2023Quote: ... or ECL anti-rabbit antibodies (NA934V; 17434242) (Amersham) were used for detection of probed proteins using enhanced chemiluminescence (ECL)(Perkin Elmer). Chemiluminescence was detected using an iBright FL1500 (ThermoFisherScientific ...
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bioRxiv - Neuroscience 2021Quote: ... The bound primary antibodies were detected by horseradish peroxidase-conjugated secondary antibodies in combination with an enhanced chemiluminescence detection system using Western Lightning Chemiluminescence Reagent (PerkinElmer Life Sciences) or ImmunoStar (Wako Pure Chemical Industries) ...
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bioRxiv - Neuroscience 2020Quote: ... Double immunofluorescence was performed using tetramethyl rhodamine labelled secondary antibody for alpha-synuclein and NSF with the fluorescein signal amplification kit (Perkin Elmer, UK). Following adequate washes to remove background fluorescence ...
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bioRxiv - Cell Biology 2021Quote: ... Horseradish peroxidase (HRP) conjugated secondary antibody was added in order for labelled proteins to be detected by Western Lightning Plus-ECL (PerkinElmer, 203-170071). Primary and secondary antibodies used are listed in Supplementary Table 5.