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Citations for PerkinElmer :
301 - 350 of 944 citations for Human Immunodeficiency Virus GP120 Protein HIV 1 Clade A KNH1144 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... cells were starved for cysteine and methionine for 15-30 min and pulse labeled for 5 min with 55 µCi/ 35-mm dish of Easytag express 35S protein labeling mix (Perkin Elmer). Where indicated (+DTT) ...
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bioRxiv - Microbiology 2021Quote: ... After these incubations the membranes were washed again with PBS-T and proteins were visualized by Western Lightning Chemiluminescence Reagent Plus (Perkin Elmer).
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bioRxiv - Immunology 2021Quote: ... The radioactive counts (cpms) of immunoprecipitated protein was quantified using a 96-well Microbeta Trilux liquid scintillation plate reader (Perkin Elmer). Antibody index for each sample was calculated as follows ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were pulse labeled for 15 minutes with 132 μCi/6 cm dish with EasyTag Express 35S Protein Labeling Mix (Perkin Elmer). Radiolabeling was stopped by adding excess ...
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bioRxiv - Cell Biology 2023Quote: ... or ECL anti-rabbit antibodies (NA934V; 17434242) (Amersham) were used for detection of probed proteins using enhanced chemiluminescence (ECL)(Perkin Elmer). Chemiluminescence was detected using an iBright FL1500 (ThermoFisherScientific ...
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bioRxiv - Cancer Biology 2023Quote: ... then each well was incubated for 30 min at 37 °C with 5 μl/well EXPRE35S35S Protein Labeling Mix (PerkinElmer NEG072002MC), after which cells were lysed in RIPA lysis buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: The recruitment of β-arrestin2 by MOR agonists after G protein activation was assessed using the Cisbio HTRF-based β-arrestin2 recruitment assays (Cisbio Assays, PerkinElmer), which monitored the interaction between β-arrestin2 and AP2 ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were pulsed with 0.1 mCi/ml 35S-L-methionine and 35S-L-cysteine mix (EasyTag™ EXPRESS35S Protein Labeling Mix, Perkin Elmer) in methionine- and cysteine-free DMEM for 15 min ...
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bioRxiv - Developmental Biology 2021Quote: ... Opal570 (1:500) and Opal650 (1:1500) using the Opal 7 Kit (NEL797001KT; PerkinElmer).
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bioRxiv - Developmental Biology 2021Quote: ... The reaction with tyramide-Cy3 (1:50 dilution in 1 ×Plus Amplification Diluent (PerkinElmer), followed by 1:100 dilution with DW ...
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bioRxiv - Genomics 2020Quote: ... Prox-1 probes were visualized by Opal 650 reagents (1:1500 dilution, Perkin Elmer). Slides were additionally co-stained with rabbit anti-human vWF polyclonal antibody (Dako ...
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bioRxiv - Immunology 2021Quote: ... 1 μCi α32P-ATP (PerkinElmer)) for 90 min at 37°C ...
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bioRxiv - Immunology 2019Quote: ... 1 s per well (PerkinElmer). The ID50 was calculated as the dilution that resulted in a 50% reduction in relative luminescence units (RLU ...
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bioRxiv - Developmental Biology 2021Quote: ... Opal570 (1:500) and Opal650 (1:1500) using the Opal 7 Kit (NEL797001KT; Perkin Elmer).
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bioRxiv - Immunology 2021Quote: ... The NIT-1 cells were then labeled with 1 μCi/well of 51Cr (Perkin Elmer) for 3 hours at 37°C and co-cultured with AI4 T cells at a E:T ratio of 20:1 for 16 hours ...
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bioRxiv - Cell Biology 2020Quote: ... 1 μCi of 2-Deoxy-D-[1-2-3H] glucose (PerkinElmer, Boston, MA, USA # NET328A001MC) and 10μM non-radioactive 2-DG (Sigma Aldrich ...
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bioRxiv - Developmental Biology 2020Quote: ... Opal570 (1:500) and Opal650 (1:1500) using the Opal 7 Kit (NEL797001KT; Perkin Elmer).
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bioRxiv - Neuroscience 2023Quote: ... Samples were diluted 1/5 or 1/10 in 1X Immunoassay buffer (Perkin Elmer, #AL000F). A 2-fold serial dilution of imiglucerase (Genzyme ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: Samples were diluted 1/5 or 1/10 in 1X Immunoassay buffer (Cat.#AL000F; PerkinElmer). For calibration ...
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bioRxiv - Microbiology 2020Quote: ... The precipitates proteins were resolved by SDS-PAGE and [32P]-labeled proteins was visualized using a Super Resolution screen (Perkin Elmer and quantified using a Cyclone Plus Storage Phosphor System (Perkin Elmer). The signal was normalized to the relative cellular content as determined by immunoblotting of whole cell lysates probed with antibodies.
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bioRxiv - Cell Biology 2021Quote: ... Horseradish peroxidase (HRP) conjugated secondary antibody was added in order for labelled proteins to be detected by Western Lightning Plus-ECL (PerkinElmer, 203-170071). Primary and secondary antibodies used are listed in Supplementary Table 5.
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bioRxiv - Cell Biology 2022Quote: ... HEK293T cells transfected with FTTTRA25T and were labeled for 30 min with EasyTag Express [35S] Protein Labeling Mix (0.1 mCi/mL; Perkin Elmer CAT# NEG772014MC) in DMEM lacking both Cys and Met (Gibco CAT# 21013024 ...
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bioRxiv - Molecular Biology 2022Quote: ... Membranes were finally washed three times with PBST for 5 min and protein levels were visualized by enhanced chemiluminescence (Perkin Elmer, NEL104001EA) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... Membranes were washed at room temperature 3 times 10 min in PBST before using chemiluminescence to reveal protein bands with the Western Lightning® Plus-ECL Enhanced Chemiluminescence reagent (PerkinElmer Inc.) as per the manufacturer’s instructions.
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bioRxiv - Cell Biology 2022Quote: Confluent 6-well dishes of FRT cells (approximately 1×106/well) were metabolically labeled in DMEM depleted of methionine and cysteine and supplemented with EasyTag 35S Protein Labeling Mix (Perkin Elmer, NEG772007MC), glutamine ...
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bioRxiv - Biophysics 2020Quote: ... HEK cell membrane proteins (100 μg/ml final concentration) were incubated with 3 nM [3H]muscimol (30 Ci/mmol; PerkinElmer Life Sciences), neurosteroid (3 nM–30 μM ...
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bioRxiv - Molecular Biology 2023Quote: ... 6 ng/ µl recombinant TopBP1 protein or buffer were added to TopBP1 immunodepleted interphase Xenopus egg extract containing 50 nCi/µl α32P-dCTP (Perkin Elmer NEG513A250UC). To start the replication reaction the egg extracts were supplemented with 5 ng sperm DNA (kindly provided by the lab of O ...
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bioRxiv - Plant Biology 2021Quote: ... 10 µM cycloheximide and 10 µCi ml-1 Na-14C acetate (PerkinElmer: 56.6 mCi mM-1) were then added to the culture ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: [3H]-DHA (1-[4,6-propyl-3H]dihydroalprenolol, specific activity 91 Ci·mmol-1) was obtained from PerkinElmer Life and Analytical Sciences (Beaconsfield ...
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bioRxiv - Microbiology 2021Quote: ... spiked with 500 nmol 3H-leucine L-1 (Perkin Elmer, specific activity 100 Ci mmol- 1) and incubated in the dark for 4 h ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and ATPlite 1 Step (Perkin Elmer). The main instruments used were the microplate reader SpectraMax 340PC (Molecular Device ...
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bioRxiv - Developmental Biology 2022Quote: ... Opal 650 (1:1500, PerkinElmer, FP1496001KT), Opal 690 (1:1500 ...
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bioRxiv - Developmental Biology 2022Quote: ... Opal 690 (1:1500, PerkinElmer, FP1497001KT). Following signal amplification of the target probes ...
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bioRxiv - Cell Biology 2022Quote: ... 1:750 TSA Cy3 (#NEL704A001KT, PerkinElmer) and TSA Cy5 (#NEL75A001KT ...
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bioRxiv - Neuroscience 2021Quote: ... Opal 570 (1:1500, Perkin Elmer), and Opal 690 (1:1000 ...
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bioRxiv - Biochemistry 2019Quote: ... 1 Ci/mol [14C]-benzylamine (PerkinElmer) and 1xPBS ...
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bioRxiv - Cell Biology 2021Quote: ... 1 μCi [14C]glycerol (Perkin Elmer) + 50 μM glycerol to stimulate glycerolipid synthesis ...
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bioRxiv - Biophysics 2023Quote: ... 1 μM 3H-L-Asp (PerkinElmer), and 0.5 μM valinomycin ...
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bioRxiv - Biophysics 2023Quote: ... 1 μL γ-32P-ATP (PerkinElmer) and 10 U T4 polynucleotide kinase (New England Biolabs ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 μM [3H]-SAM (Perkin Elmer), 1 μM full-length C ...
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bioRxiv - Biochemistry 2024Quote: ... 1 μL γ-32P-ATP (PerkinElmer) and 10 U T4 polynucleotide kinase (New England Biolabs ...
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bioRxiv - Genomics 2019Quote: ... Anti-rabbit or anti-mouse HRP conjugated antibodies were used to detect protein by chemiluminescence using ECL (Perkin Elmer, Western Lightning Plus ECL). Images were visualized by GeneSnap image analysis software (SynGene ...
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bioRxiv - Cancer Biology 2020Quote: ... containing 10% BSA and 3 µl [35S] EasyTag Express protein labelling mix (7 mCi, 259 MBq, 1175 Ci/mmol in 632 ml; Perkin Elmer, Espoo, Finland). After 2 h of incubation at 37 °C cells were washed twice with PBS ...
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bioRxiv - Biochemistry 2023Quote: ... Ten μl of these dilutions or 10 μl of G protein storage buffer without nucleotides were pipetted into the corner of a white opaque 96-well plate (Opti-Plate, PerkinElmer Life Sciences, 6005290). Ten 10 μl of 0.1 mM Coelenterezine 400a diluted in BRET buffer (140 mM NaCl ...
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bioRxiv - Genomics 2020Quote: ... LETR1 and Malat-1 RNAscope probes were visualized by Opal 570 reagents (1:1500 dilution, Perkin Elmer); Prox-1 probes were visualized by Opal 650 reagents (1:1500 dilution ...
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bioRxiv - Microbiology 2019Quote: ... 50μM ATP) with 200 µCi ml−1 (65 nM) [γ-33P]ATP (PerkinElmer, 3000 Ci mmol−1) for 30 min at 37 °C ...
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bioRxiv - Bioengineering 2023Quote: ... for SARS-CoV-2 S protein neutralization antibody titer in the wells was observed using the High Content Analysis System (Perkin Elmer, Waltham, MA, USA) or microscope ...
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bioRxiv - Cell Biology 2020Quote: ... 2DG transport was measured together with 1 mM 2DG during the last 10 min of the contraction stimulation period using 0.60-0.75 µCi mL-1 [3H]-2DG and 0.180-0.225 µCi mL-1 [14C]-mannitol radioactive tracers (Perkin Elmer) as described previously 31 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... amplified by a streptavidin-horseradish peroxidase conjugate (1:300) and detected with TSA tyramid-Cy3 (1:50, PerkinElmer). All sections were counterstained with DAPI (1:1000 ...
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bioRxiv - Microbiology 2022Quote: ... Samples were amended with 100 nmol of leucine L-1 (Perkin Elmer, specific activity 156 Ci mmol-1) and incubated for 4 h in the dark under ambient surface sea water temperature (∼19 °C) ...