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Citations for PerkinElmer :
301 - 350 of 378 citations for H D Glu amc oh since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Expression of firefly luciferase was determined 24 h after transfection using the Britelite plus Reporter Gene Assay System (PerkinElmer Inc., Milan, Italy) at a Victor X2 multilabel plate reader (PerkinElmer Inc. ...
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bioRxiv - Immunology 2020Quote: ... and allowed to refold at 18°C for 24 h in a Streptavidin-coated scintillation microplate (Flashplate PLUS, Perkin Elmer, Boston, MA). Dissociation was initiated by adding excess unlabeled β2m and placing the microplate in a scintillation counter (TopCount NXT ...
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bioRxiv - Microbiology 2021Quote: ... Viral supernatant was then used to transduce each cell line of interest for 48 h and luciferase activity was measured with Promega Steady-Glo® kit (E2550) using a Victor X3 Multilabel Reader (Perkin Elmer). Each experiment shown is representative of three independent experiments.
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bioRxiv - Immunology 2020Quote: ... The plates were further incubated for 48 h and the extent of virus neutralization was assessed by measuring relative luminescence units in a luminometer (Victor X2, PerkinElmer Life Sciences).
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bioRxiv - Immunology 2023Quote: ... Fluorescent signals were monitored in real-time with a microplate reader for 24 h within a tissue culture incubator (37 °C, 5 % CO2) on black OptiPlate 96-well (PerkinElmer, MA, USA) under 20x objective ...
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bioRxiv - Cancer Biology 2023Quote: ... Intracellular GSH and GSSG levels were determined after 48 h of FR054 and erastin in single or combined treatment by VICTOR X3 multimode plate reader (PerkinElmer, Milan, Italy) using a Total GSH / GSSG colorimetric kit (CO-K097-M ...
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bioRxiv - Cell Biology 2023Quote: ... 5 μl cAMP Eu-cryptate reagent and 5 μl of anti-cAMP-d2 working solution were added to the 384-well plates and incubated for 1 h.54 Fluorescence signals were detected at 620/665 nm using the Multimode Plate Reader (PerkinElmer EnVision 2105).55 Data were analyzed with GraphPad Prism 9.0 ...
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bioRxiv - Molecular Biology 2024Quote: ... and 2.3±0.64 pmol/mg protein (Bmax for β 2AR) and binding affinities for the radioligand [³H]CGP12,177 (specific activity 51 Ci/mmol, PerkinElmer, Rodgau, Germany) of 0.070 nM (KD for β1AR ...
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bioRxiv - Cancer Biology 2024Quote: ... The cells were then pulsed for 1 h with 200 μCi/ml of [35S]-Met(methionine)/[35S]-Cys(Cysteine) (Perkin Elmer, NEG072002MC) and chased for 0 ...
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bioRxiv - Cancer Biology 2021Quote: ... Bioluminescent imaging was performed every 3-7 days following intraperitoneal injection of D-luciferin and measured using the Xenogen IVIS Spectrum in vivo imaging system (PerkinElmer). Living Image software (PerkinElmer ...
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bioRxiv - Immunology 2021Quote: ... The luciferase signal was determined in live animals one day after virus injection and 10 minutes after intraperitoneal injection of 150 mg/kg D-luciferin (PerkinElmer) using the in vivo imaging system NightOWL II LB 983 (Berthold ...
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bioRxiv - Physiology 2020Quote: On the fifth day of EOS or PBS treatment 3H-2-deoxy-D-glucose (2DG; 10uCi/mouse; PerkinElmer, Waltham, MA) was given to 4 hour fasted individually housed mice ...
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bioRxiv - Bioengineering 2021Quote: ... We verified luciferase expression in selected stable cells after providing 150 ug/ml D-luciferin to the growth media and detecting bioluminescence using an IVIS In Vivo Imaging System (PerkinElmer).
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bioRxiv - Biochemistry 2021Quote: ... The viral infectivity was measured by quantifying luciferase activity using 150ug/ml of XenoLight D-Luciferin bioluminescent substrate (PerkinElmer # 122799). Data was plotted using GraphPad Prism software.
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bioRxiv - Cancer Biology 2021Quote: ... Bioluminescent imaging was performed weekly following intraperitoneal injection of D-luciferin and measured using the Xenogen IVIS Spectrum in vivo imaging system (PerkinElmer). Living Image software (PerkinElmer ...
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bioRxiv - Molecular Biology 2022Quote: Undifferentiated H1-OCT4-GFP-INS:tdT-luciferase hPSC and the differentiated β-like cells were incubated with 15mg/mL D-luciferin for 1 min before imaging with an IVIS Spectrum system (PerkinElmer), with an exposure of 1 min and mid-bin setting ...
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bioRxiv - Cancer Biology 2020Quote: ... Leukemia burden was serially assessed using noninvasive bioluminescence imaging by injecting mice intraperitoneally (i.p.) with 150 mg/kg D-Luciferin (PerkinElmer, 122799) in phosphate-buffered saline (PBS ...
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bioRxiv - Cancer Biology 2020Quote: ... Mice were anesthetized with 3% isoflurane before retro-orbital injection with d-luciferin (150mg/Kg) (Perkin Elmer S.L., Cat. 122796) and imaged with an IVIS Xenogen machine (Caliper Life Sciences) ...
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bioRxiv - Neuroscience 2023Quote: ... and imaged 10 minutes after D-luciferin injection with a cooled charge-coupled device (CCD) camera (In Vivo Imaging System by PerkinElmer). Mice were imaged regularly every week until week 8 and every 4 weeks until week 28 ...
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bioRxiv - Cancer Biology 2022Quote: ... as determined by administration of 150 mg/kg D-luciferin and measurement of tumour luminescence on an IVIS SpectrumCT imager (PerkinElmer). Star 3 + control siRNA (antisense ...
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bioRxiv - Immunology 2024Quote: ... Tumor engraftment was confirmed on day 6 via intraperitoneal injection of IVISbrite D-Luciferin Potassium Salt Bioluminescent Substrate (XenoLight, PerkinElmer), followed by bioluminescent imaging (BLI ...
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bioRxiv - Bioengineering 2023Quote: ... animals were imaged with IVIS Kinetic (Caliper Life Sciences) using an intraperitoneal injection of 0.2 μm filtered Xenolight D-luciferin (Perkin Elmer) at 150 mg/kg body weight ...
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bioRxiv - Cancer Biology 2023Quote: ... mice were given 10 μL/g of D-luciferin (15 mg/mL) (Gold Bio) intraperitoneally and imaged using the IVIS Spectrum imager (PerkinElmer). Luciferase signal was quantified 15 min after injection ...
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bioRxiv - Cancer Biology 2022Quote: ... after the intraperitoneal injection of luciferin (150 mg of luciferin/kg; XenoLight™ D-Luciferin K+ Salt Bioluminescent, Perkin Elmer). Metastasis development was monitored every 3-4 days until 18 days when mice were sacrificed by CO2 inhalation and their lungs were extracted ...
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bioRxiv - Cancer Biology 2023Quote: ... Luciferase measurements were taken every other day beginning from day 2 (the day after the injection) – mice were anaesthetised (isoflurane inhalation) and subcutaneously injected with Xenolight D-luciferin substrate (PerkinElmer). Mice were imaged after 5min using the IVIS Lumina II (Caliper Life Sciences) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 5E4 cells per well were seeded in 100 µL of growth medium into poly-D-lysine-coated white 96 well OptiPlates (PerkinElmer). The following day ...
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bioRxiv - Immunology 2024Quote: ... tumor implantation was confirmed by measuring tumor bioluminescence after the intraperitoneal (IP) injection of 150 mg/kg of D-luciferin using the In Vivo Imaging System (IVIS) (PerkinElmer). Randomization into the treatment groups was performed based on the BLI signal analyzed with Living Image Software (Revvity ...
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bioRxiv - Immunology 2024Quote: ... Tumor burden was monitored longitudinally twice per week (or once per week if mice reached long-term remission) by intraperitoneal injection of IVISbrite D-Luciferin substrate (PerkinElmer) at a dose of 0.15 mg per gram of body weight ...
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bioRxiv - Neuroscience 2024Quote: ... and transfected cells were plated evenly across 10 wells of poly-D-lysine-coated 96-well PhenoPlates (PerkinElmer; Cat# 6055508). Half-media changes of BMDM medium containing 50ng/mL M-CSF were performed every other day and endpoints were assessed until 14 days after transfection.
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bioRxiv - Cancer Biology 2024Quote: ... Metastatic burden was monitored by bioluminescence imaging (BLI) using retro-orbital injection of D-luciferin (150 mg/kg) and an IVIS Spectrum Xenogen instrument (PerkinElmer). Data were analyzed using Living Image software v4.5 (PerkinElmer) ...
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bioRxiv - Cell Biology 2022Quote: ... Luminescence was measured at 1 h post PathHunter© detection reagent addition using Victor3 plate reader (Perkin Elmer 0.5-s/well integration time). Data were normalized to control treated with the highest concentration of ligand (100%) ...
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bioRxiv - Cell Biology 2021Quote: ... sections were washed in TBST and incubated with Alexa Fluor® 594 AffiniPure Goat Anti-Rabbit IgG (H+L) (Abacus DX, Australia) with Spectral DAPI (FP1490) added (1/1000) (Perkin Elmer, USA) for one hour at room temperature ...
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bioRxiv - Immunology 2021Quote: ... was performed repetitively in short-term isoflurane anesthesia over a period of 24 h using the IVIS Spectrum In Vivo Imaging System (PerkinElmer, Waltham, MA, USA). Four days after the first Nb administration ...
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bioRxiv - Molecular Biology 2020Quote: ... The effects of the compounds were evaluated 24 h later using an Operetta CLS Confocal High Content Imaging System (PerkinElmer, Waltham, Massachusetts, USA) with the following parameters ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... lung tissues harvested from euthanized mice were soaked for 10 min in pre-warmed (37°C) PBS with 2% FBS and a 1:10 dilution of Xenolight RediJect Coelenterazine h (Cat# 706506, Perkin Elmer, Waltham, MA). Following a 12-15 min incubation ...
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bioRxiv - Immunology 2024Quote: ... Luciferase activity was quantified after 48–72 h by the addition of Steadylite Plus chemiluminescence substrate and analysis on a Perkin Elmer EnSight multimode plate reader (Perkin Elmer, Beaconsfield, UK). Antibody titre was then estimated by interpolating the point at which infectivity had been reduced to 50% of the value for the ‘no serum’ control samples.
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bioRxiv - Cancer Biology 2023Quote: ... and the plate was incubated in the dark for 2 h and then read in an EnSpire Alpha multimode plate reader (PerkinElmer, Waltham, MA, USA).
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bioRxiv - Immunology 2022Quote: ... bound cytokines were detected by biotinylated anti-IFN-γ (clone 4s.B3) and quantified using a Victor2 D time-resolved fluorometer (Perkin Elmer). The % activity was calculated by dividing the SI of mutated peptides by the SI of the wild-type peptide ...
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bioRxiv - Microbiology 2020Quote: ... log phase promastigotes were washed twice with PBS and resuspended at 5.0 x 107 cells/ml in PBS in the presence of 100 µM of D-[U-14C] glucose (250 mCi/mmol, PerkinElmer Inc.). The reaction mixture (200 µl each in triplicates ...
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bioRxiv - Physiology 2021Quote: ... a longer time was required to achieve steady state) a single bolus of 12 μCi of [1-14C]-2-deoxy-D-glucose (Perkin Elmer) in 96 μl of saline was administered ...
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bioRxiv - Physiology 2020Quote: On the fifth day of EOS or PBS treatment 3H-2-deoxy-D-glucose (2DG; 10 uCi/mouse; PerkinElmer, Waltham, MA) was given to 4-hour fasted individually housed mice ...
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bioRxiv - Immunology 2021Quote: ... Liver luminescence was assessed 42 hours post-challenge by intraperitoneally injecting anesthetized mice with 100 μl D-luciferin (30mg/ml) and then determining liver luminescence using an IVIS Spectrum Imaging System (Perkin Elmer). Beginning four days after challenge ...
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bioRxiv - Cell Biology 2023Quote: ... and 4,000 cells/well (for G2/M phase nocodazole treatment) with a complete media overnight onto poly-D-lysine-coated CellCarrier-Ultra-96 well Black (Perkin Elmer).
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bioRxiv - Cancer Biology 2022Quote: ... Imaging of grafted tumors was performed using an IVIS in vivo imager (LAGO) after intraperitoneal injection of RediJect D-Luciferin Bioluminescent Substrate (PerkinElmer, #770504) 13 weeks after injection ...
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bioRxiv - Cancer Biology 2022Quote: ... Imaging of grafted tumors was performed monthly using an IVIS in vivo imager (LAGO) after intraperitoneal injection of RediJect D-Luciferin Bioluminescent Substrate (PerkinElmer, #770504).
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bioRxiv - Microbiology 2023Quote: ... the challenged mice were intraperitoneally injected with 100 μL of D-luciferin (30 mg/mL) (Perkin Elmer, Waltham, MA, USA, #122799), and then anesthetized in an isoflurane chamber ...
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bioRxiv - Cancer Biology 2023Quote: ... into female C57BL/6J (JAX strain #000664) mice and tumor burden was measured by bioluminescence (PerkinElmer IVIS Spectrum In Vivo Imaging System, D-luciferin PerkinElmer #122799). Data was analyzed using Living Image software.
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bioRxiv - Physiology 2023Quote: ... the animals were fasted overnight and a bolus of 2-deoxy-D-[1,2-3H]-glucose (1mC/ml; specific activity: 20Ci/mmol; PerkinElmer, Madrid, Spain) mixed with glucose (100µCi/kg body weight ...
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bioRxiv - Immunology 2024Quote: ... Pups were injected intraperitoneally with D luciferin (150 mg/kg) at 6 hours after LNP injection and imaged using an IVIS Lumina system (Perkin Elmer). To evaluate the in vivo response to CGT ODNs ...
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bioRxiv - Cancer Biology 2023Quote: ... LN-229 cells were seeded at a density of 70,000 cells/well on poly-D-Lysine-coated ViewPlateTM-96 F TC 96-well black polystyrene clear bottom microplates (PerkinElmer, #6005182) in 100µl full medium ...