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Citations for PerkinElmer :
301 - 350 of 569 citations for DNA Mismatch Repair Protein Msh3 MSH3 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... The final Western blotting bands were visualized using an enhanced chemiluminescence protein detection system (PerkinElmer Life Sciences, Boston, MA).
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bioRxiv - Molecular Biology 2021Quote: ... 50 μL/well) was captured overnight at 4°C onto high-protein-binding ELISA plates (Spectraplate 96HB, Perkin Elmer). Plates were washed in PBS/Tween (0.05% v/v ...
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bioRxiv - Cancer Biology 2020Quote: ... shRNA expression with surrogate red fluorescent protein (RFP) was tracked by fluorescence (IVIS Spectrum In Vivo Imaging Station, PerkinElmer). For chloroquine xenograft experiments ...
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bioRxiv - Cell Biology 2021Quote: ... Media were replaced with fresh media supplemented with Easytag Express S35 Protein Labeling Mix (PerkinElmer, 100-200 µCi/mL) for 1 h ...
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bioRxiv - Neuroscience 2022Quote: ... The images of the targeting proteins were analyzed by a high content screening system (Operetta Enspire, Perkin Elmer, USA). The integral optical density (IOD ...
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bioRxiv - Microbiology 2022Quote: ... The membranes were incubated with primary and secondary HRP-conjugated antibodies in PBST (5% milk and 0.1% Tween-20) to detect the protein of interest by Western Lightning Plus ECL (PerkinElmer) and ImageQuant LAS 4000 imaging system (GE) ...
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bioRxiv - Cell Biology 2021Quote: ... cells were metabolically labelled for 20 min with 55 μ Ci/ml 35S- methionine/cysteine protein labelling mix (PerkinElmer). Cells were washed with PBS and cultured in DMEM (Invitrogen ...
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bioRxiv - Neuroscience 2021Quote: The level of MMP-9 in plasma was measured with an AlphaLISA kit for the human protein (#AL3138, PerkinElmer). AlphaLISA signals were measured using an Ensight Multimode Plate Reader (PerkinElmer).
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bioRxiv - Immunology 2021Quote: ... The SARS-CoV-2 spike protein antigen (2.0µg/mL) was directly adsorbed on half-area 96-well OptiPlate (96W HB, Perkin Elmer) microplates for 2h at 37°C in a humidified incubator.
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bioRxiv - Immunology 2020Quote: ... The cpms of immunoprecipitated protein was quantified using a 96-well Microbeta Trilux liquid scintillation plate reader (Perkin Elmer).
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... We next added 0.8 μl of FLAG-GST-transcription factor proteins to 384-well AlphaPlates using a NanoHead (PerkinElmer) and a Janus Workstation (PerkinElmer) ...
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bioRxiv - Biochemistry 2021Quote: ... 20 μL of protein mixture was added to each well of a 1/2 area 96-well OptiPlate (PerkinElmer). Compounds were then added from DMSO stock to protein mixture (0.4 μl/well ...
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bioRxiv - Biochemistry 2021Quote: ... 20 μL of protein mixture was added to each well of a 1/2 area 96-well OptiPlate (PerkinElmer). FKBP12F36V competitor or p300-BRD competitor was then added from DMSO stock to protein mixture (0.4 μl/well ...
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bioRxiv - Microbiology 2021Quote: ... was used (at 1:20.000 dilution) for protein visualization in the presence of Western Lightning Plus-ECL enhanced chemiluminescence substrate (PerkinElmer). The WesternSure® Pre-stained Chemiluminescent Protein Ladder (Li-cor ...
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bioRxiv - Neuroscience 2022Quote: GPCR activation assays were performed using CHO-K1 cells stably expressing the luminescent Ca2+ indicator aequorin and the promiscuous Gα16 protein (ES-000-A24 cell line, PerkinElmer). The cells were transfected with a dmsr-7/pcDNA3.1 plasmid using lipofectamine LTX and Plus reagent (Invitrogen ...
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bioRxiv - Biochemistry 2023Quote: ... 10 μM purified IKK2 protein was first autophosphorylated in the presence of 100-200 nM of γ32P-ATP (PerkinElmer) in kinase assay buffer for 1 hour at 30°C ...
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bioRxiv - Microbiology 2022Quote: ... cells were metabolically labeled for 16 h with 100 μCi/mL [35S] methionine-cysteine ([35S] protein labeling mix; PerkinElmer) in Dulbecco’s modified Eagle’s medium lacking methionine and cysteine and supplemented with 10% of dialyzed fetal bovine serum and 1X GlutaMAX™ (ThermoFisher Scientific) ...
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bioRxiv - Biochemistry 2022Quote: ... Cells then were radiolabeled for 15 minutes with 143 µCi/dish EasytagTM 35S Express Protein Labeling Mix (Perkin Elmer) and chased for indicated times in DMEM supplemented with 5 mM unlabeled methionine and cysteine ...
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bioRxiv - Physiology 2022Quote: ... 60-100 µg protein was incubated with 100 μl TG substrate (25 nmol triolein/assay and 40,000 cpm/nmol 14C-triolein; PerkinElmer) and 35.5 μg mixed micelles of phosphatidylcholine and phosphatidylinositol (3:1 ...
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bioRxiv - Cell Biology 2023Quote: ... aliquots of derivatized lysates containing 15 µg of protein were spotted on a Whatman Protran nitrocellulose membrane (Perkin Elmer). Blots were blocked as above ...
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bioRxiv - Molecular Biology 2024Quote: ... after 20 min proteins newly synthetized in the mitochondrial compartment were labeled with 100 μCi [35S]-methionine (Perkin Elmer) for 1 h at 37 °C ...
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bioRxiv - Neuroscience 2024Quote: ... ± drug for 30 minutes then slices were metabolically labelled in 35S-Methionine/Cysteine express protein labeling mix (Perkin Elmer) ± drug for 30 minutes ...
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bioRxiv - Biochemistry 2021Quote: ... and then incubated with anti-FLAG antibody conjugated to APC (αFLAG-APC; PerkinElmer) diluted 1:100 in PBS + 0.1% BSA at a ratio of 30 μL labeled antibody:107 cells ...
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bioRxiv - Neuroscience 2022Quote: ... Antibody binding was detected using the enhanced chemiluminescence immunoblotting detection system (Perkin Elmer LLC WESTERN LIGHTNING PLUS-ECL ...
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bioRxiv - Cell Biology 2022Quote: ... Bound antibodies were detected with chemiluminescent substrate (Western Lightning® Plus, Perkin Elmer) and visualized with iBrightTM CL1000 (Thermo Fisher Scientific).
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bioRxiv - Immunology 2020Quote: ... The antibodies were visualized by incubation with Tyramide Red or Green (Perkin Elmer) for 10 minutes ...
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bioRxiv - Genetics 2021Quote: ... horseradish peroxidase-conjugated secondary antibodies and Western Lightining® Plus-ECL (NEL105001EA, PerkinElmer) were used.
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bioRxiv - Immunology 2022Quote: ... Unspecific antibody binding on sorted cells was blocked with TSA blocking reagent (PerkinElmer) for 30 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... primary antibodies followed by HRP-conjugated secondary polymer (anti-rabbit, anti-mouse [PerkinElmer]) and HRP-reactive OPAL fluorescent reagents (PerkinElmer) ...
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bioRxiv - Biochemistry 2024Quote: ... Acceptor mixtures were prepared by mixing ULight alpha-6xHIS acceptor antibody (Perkin Elmer) with 6xHIS-tagged Swc5 variants at a ratio of 1:20 and serially diluting across 13 concentrations in H66 buffer (20 mM HEPES pH 7.5 ...
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bioRxiv - Neuroscience 2023Quote: ... membranes were incubated with goat anti-rabbit HRP- labelled antibody (Perkin Elmer, # NEF812001EA) at 1/10000 in 1 % (w/v ...
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bioRxiv - Cancer Biology 2022Quote: ... horseradish peroxidase-conjugated secondary antibodies and Western Lightining® Plus-ECL (NEL105001EA, PerkinElmer) were used ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were washed with TBST and incubated with secondary antibody (NEF812001EA, NEF822001EA, PerkinElmer) for 1 h ...
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bioRxiv - Cell Biology 2023Quote: ... HRP-conjugated antibodies were detected using western lighting plus-ECL reagent (Perkin elmer).
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bioRxiv - Evolutionary Biology 2021Quote: ... with 15 cycles of PCR and using 1:125 dilution of NEXTflex DNA barcodes (Perkin Elmer,NOVA-514104). The resulting libraries were purified using the Agencourt AMPure XP system (Beckman Coulter ...
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bioRxiv - Biochemistry 2020Quote: ... Labeling reactions were designed to use equimolar amounts of DNA and γ-32P-ATP (Perkin Elmer cat. # NEG035C001MC) and consisted of 37.5 pmol (7.5 μl of 5 μM ...
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bioRxiv - Neuroscience 2020Quote: ... DNA samples and blank were measured in duplicate using EnSpire® Multimode Plate Reader (PerkinElmer Inc, Waltham, USA). By plotting the measured fluorescence versus DNA ...
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bioRxiv - Genomics 2020Quote: DNA from saliva collected with mouthwash samples was extracted on a chemagic platform (PerkinElmer chemagen Technologie GmbH, Germany). DNA collected with Oragene OG500 Kits (DNA Genotek Inc. ...
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bioRxiv - Genetics 2020Quote: ... and fragmented DNA was ligated to index adapters and amplified (BioO NEXTflex qRNA-Seq Kit v2, Perkin Elmer). Indexed DNAs smaller than 500 bp were gel purified and subjected to next generation sequencing (HiSeq 4000 ...
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bioRxiv - Immunology 2023Quote: ... Libraries were QC’ed using the DNA High Sensitivity Assay on the LabChip GX Touch (Perkin Elmer, Waltham, MA). Library concentrations were also assessed by qPCR using the KAPA Library Quantification Kit (Complete ...
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bioRxiv - Genomics 2022Quote: ... was used for NGS library construction using a NEXTflex Rapid DNA-Seq Kit 2.0 (PerkinElmer, Inc., Waltham, MA). Exome regions were enriched via hybridization with an IDTexome panel (Integrated DNA Technologies ...
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bioRxiv - Microbiology 2024Quote: ... Viral RNAs were detected with DNA oligonucleotides (Table S2) end-labelled with [32P] γ-adenosine-triphosphate (Perkin Elmer) using T4 polynucleotide kinase (Roche) ...
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bioRxiv - Physiology 2024Quote: ... and size pattern is controlled with the DNA High Sensitivity Reagent kit on a LabChip GX (Perkin Elmer). Libraries are pooled at an equimolar ratio (i.e ...
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bioRxiv - Cell Biology 2024Quote: ... with a dsDNA High-Sensitivity Assay kit and LabChip GX Touch using a High-Sensitivity DNA chip (PerkinElmer). Libraries were then pooled at equimolar concentrations and quantified by qPCR using a KAPA library quantification kit (Roche) ...
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bioRxiv - Cancer Biology 2021Quote: ... 30 ug of protein lysate was resolved by SDS-PAGE and transferred onto a Protran pure 0.2 micron nitrocellulose (Perkin Elmer). The membranes were incubated for 1h in 5% skimmed milk and probed with specific primary antibodies (Table S1 ...
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bioRxiv - Cell Biology 2020Quote: ... and labeled for 5 min with with 1 μL per OD of cells of EXPRESS 35S Protein Labeling Mix (PerkinElmer) for 5 or 10 min ...
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bioRxiv - Immunology 2021Quote: ... the unbound proteins were washed and luciferase activity was measured using Nano-Glo substrate (Nanolight) in an Envision 2103 microplate luminometer (PerkinElmer).
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bioRxiv - Genetics 2021Quote: ... Grain protein content (%) and grain yield (t/ha) was collected using a Perten DA 700 NIR analyzer (Perkin Elmer, Sweden) and Wintersteiger Nursery Master combine (Ried im Innkreis ...
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bioRxiv - Molecular Biology 2020Quote: ... The protein pellet was then re-suspended in PBS and incubated in 4 mL of scintillation fluid (Insta-Gel Plus, PerkinElmer) for 24 h (to homogenize the samples ...
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bioRxiv - Microbiology 2021Quote: ... 1.37 μg/mL of RBD and 5 μg/mL of Ecto) and incubated overnight in protein-binding 96-well plates (PerkinElmer). The next day ...