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Citations for PerkinElmer :
251 - 300 of 1076 citations for OX7 Anti Thy 1 Mouse Monoclonal biotin labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... purified mitochondria were labeled with 30 μCi of alpha-32P UTP (Perkin Elmer BLU007H250UC) in transcription buffer (10 mM Tris pH 7.5 ...
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bioRxiv - Cancer Biology 2023Quote: ... The probe was labeled on the 5′ end with [γ-32P] ATP (Perkin Elmer) using T4 polynucleotide kinase (NEB M0201 ...
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bioRxiv - Genetics 2023Quote: ... and end-labeled with (γ-P32) adenosine triphosphate (MP Biomedicals or Perkin Elmer, #NEG002A250U) using T4 polynucleotide kinase (NEB ...
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bioRxiv - Physiology 2020Quote: ... Blots were then washed with TBST prior to incubation with HRP-linked anti-mouse secondary antibody (Perkin Elmer) for 1 h at room temperature ...
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bioRxiv - Evolutionary Biology 2019Quote: ... animals were incubated overnight with peroxidase-conjugated antibodies at 4°C (anti-DIG-POD, Roche 1:500 dilution and anti-DNP, Perkin Elmer, 1:200 dilution) followed by the amplification of the signal with fluorophore-conjugated tyramides (1:100 TSA reagent diluents ...
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bioRxiv - Cell Biology 2020Quote: ... K562 cells (5×106/ml) were labeled with 200 μCi of 51Cr (PerkinElmer, Wellesley, MA). After labeling ...
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bioRxiv - Developmental Biology 2020Quote: ... After detection of digoxigenin-labeled probes by deposition of TSA Plus fluorescein solution (Perkin Elmer), embryos were incubated with an AlexaFluor 647 goat anti-mouse IgG secondary antibody (Invitrogen ...
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bioRxiv - Biochemistry 2020Quote: ... the 25-nt oligonucleotide was labeled at the 5’ end by γ-32P ATP (PerkinElmer) using T4 polynucleotide kinase (NEB).
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bioRxiv - Cancer Biology 2022Quote: ... The sections were labeled by using the Opal 7-Color fIHC Kit (PerkinElmer, Waltham, MA). The antibody fluorophores were grouped into a panel of 5 antibodies ...
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bioRxiv - Microbiology 2023Quote: ... The template was labeled with the kit by adding 10 μCi alpha-CTP (Perkin Elmer) to the reaction for four hours at 37°C ...
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bioRxiv - Genetics 2023Quote: ... Fluorescence was measured every 60 s using a multi-labeled microplate detector (Perkin Elmer Envision). Depolymerizing activities associated with the test proteins were identified by comparing the depolymerization curves ...
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bioRxiv - Microbiology 2019Quote: ... which were incubated with various concentration of synthetic biotinylated TAR-RNA (biotin 5’-dT/GGU CUC UCU GGU UAG ACC AGA UCU GAG CCU GGG AGC UCU CUG GCU AAC UAG GGA ACC/3’-biotin dT) (Integrated DNA Technologies) at 4°C for 3 h in the AlphaLISA buffer (PerkinElmer). Anti-His Donor and Streptavidin Acceptor AlphaLISA beads (PerkinElmer ...
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bioRxiv - Genomics 2021Quote: ... Aliquots of 10E6 nuclei were subjected to 3-minute nuclear run-on reactions in the presence of Biotin-11-CTP (PerkinElmer) and PRO-seq libraries were constructed in duplicate as described (31) ...
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bioRxiv - Genomics 2022Quote: ... PRO-seq libraries for all replicates were then prepared simultaneously by subjecting one aliquot of 1e7 nuclei/sample to 3-minute nuclear run-on reactions in the presence of Biotin-11-CTP (PerkinElmer) following our previously detailed protocol (24) ...
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bioRxiv - Genomics 2022Quote: ... nuclei were extracted from ~2×107 cells and run-on reactions were carried on at 37°C for 3 minutes using 3μl of each 11-biotin-labelled ribonucleotide stocks (Perkin Elmer). Following real time PCR amplification in the presence of EvaGreen dye as above ...
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bioRxiv - Genomics 2023Quote: The nuclear run-on experiments for PRO-seq were performed in triplicate as described in 47 with the use of a mixture of rNTPs and Biotin-11-CTP (PerkinElmer), and a range of 10-20 million isolated nuclei per sample ...
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bioRxiv - Developmental Biology 2020Quote: ... Probes were labeled with digoxigenin and detected by deposition of TSA Plus fluorescein solution (Perkin Elmer). Stained embryos were washed in PBT and mounted in SlowFade Gold anti-fade reagent with DAPI (Molecular Probes ...
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bioRxiv - Microbiology 2020Quote: ... Cultures were labeled with 80 µCi/mL [35S] EasyTag™ EXPRESS35S Protein Labeling Mix (Perkin Elmer) for 10 min ...
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bioRxiv - Developmental Biology 2020Quote: ... The restriction fragments of each region were then gel purified and labeled with [α32P]-dGTP (PerkinElmer) using the large fragment of DNA polymerase I (Invitrogen) ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were then labeled with 0.1 mCi/mL EasyTag EXPRESS 35S Protein Labeling Mix (Perkin Elmer) diluted in labeling media ...
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bioRxiv - Microbiology 2022Quote: ... Northern probes were 5’ 32P-end labeled with 0.3 mCi of y-32P ATP (Perkin Elmer) with 10 U of T4 Polynucleotide kinase (NEB ...
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bioRxiv - Molecular Biology 2022Quote: ... blots were hybridized with RNA probes in vitro transcribed with 32P-labeled UTP (PerkinElmer Life Sciences). Radioactive signals were detected with a phosphorimager and signals were quantified with Quantity ONE software (Bio-Rad).
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bioRxiv - Neuroscience 2023Quote: ... The DIG-labeled probes were amplified with horseradish peroxidase using Cy3.5 tyramide signal amplification kit (PerkinElmer) for 5 to 10 min.
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bioRxiv - Developmental Biology 2023Quote: ... The annealed synthetic oligonucleotide DNA was labeled with [α-32P]dCTP (Perkin Elmer NEG013H250UC, 3000Ci/mmol) by Klenow polymerase ...
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bioRxiv - Biochemistry 2022Quote: ... 1 nM LANCE Ultra Europium anti-rabbit IgG antibody (PerkinElmer AD0082), and 50 nM LANCE Ultra Ulight-Streptavidin (PerkinElmer TRF0102 ...
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bioRxiv - Biochemistry 2024Quote: ... HRP labelled goat anti-rabbit IgG (Perkin Elmer: NEF812001EA, 1:10,000), StarBright Blue 520 goat anti-mouse IgG (Bio-Rad 64456855 ...
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bioRxiv - Neuroscience 2022Quote: ... then incubated for 30 min at 25 °C with shaking at 400 RPM with 9 ng/well of DELFIA Eu-N1 anti-mouse antibody in DELFIA assay buffer (PerkinElmer), washed with 3 × 100 μl of PBST before enhancing with 80 μl of DELFIA Enhancement Solution (PerkinElmer) ...
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bioRxiv - Molecular Biology 2021Quote: ... metabolically labeled for 20 min with 35S-Met (50 μCi 35S-Met, 1175 Ci/mmol, Perkin Elmer) and then lysed in SDS-lysis buffer (25 mM Tris-HCl pH 7.5 ...
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Transcription factor LSF facilitiates lysine methylation of α-tubulin by microtubule-associated SET8bioRxiv - Biochemistry 2019Quote: ... or recombinant MBP-β-tubulin were incubated with 6 μM radioactively labeled [3H] AdoMet (Perkin Elmer #NET155V001MC) in 1X HMT buffer containing 5 mM Tris pH 8.0 ...
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bioRxiv - Cancer Biology 2020Quote: Imaging of fluorescently labeled tissue sections was performed utilizing Nuance-FX multispectral imaging system (Caliper LifeSciences, PerkinElmer), using full capabilities of its Tunable Liquid Crystal imaging module ...
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bioRxiv - Biochemistry 2021Quote: ... 100 pmol of RNA were 5’-end labeled with [γ- 32P]-ATP (3000 Ci/mmol, Perkin Elmer) using T4 polynucleotide kinase (NEB ...
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bioRxiv - Molecular Biology 2022Quote: ... 10mM sodium butyrate and 0.25 µCi/µl (4.9 Ci/mmol) of [3H]-labeled Acetyl-CoA (Perkin Elmer) or 0.15mM unlabeled Acetyl-CoA (Sigma) ...
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bioRxiv - Molecular Biology 2022Quote: DNA probes (U1-SH, U4-14B, and U6-SH) were end labeled with [γ-32P]-ATP (PerkinElmer) using T4 PNK (Thermo Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... Riboprobes were labeled with either digoxigenin-UTP or dinitrophenyl-11-UTP (DNP; Perkin Elmer, Waltham, MA, USA). A DNP-labeled probe and a DIG-labeled probe were hybridized simultaneously ...
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bioRxiv - Microbiology 2019Quote: ... while the 14C-labeled methyladenosines were detected with a Packard radiomatic 515TR flow scintillation analyzer (Perkin Elmer).
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bioRxiv - Microbiology 2019Quote: ... was prepared and labeled according to manufacturer’s guidelines using [gamma- 32P]-ATP (6,000 Ci/mmol) (Perkin Elmer) and T4 Polynucleotide Kinase (PNK ...
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bioRxiv - Genomics 2021Quote: ... Double-stranded DNA probes were prepared by end-point PCR and labeled with dCTP [α-32P] (PerkinElmer) using the Cytvia Amersham Megaprimer labeling kit (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2020Quote: ... DNA methylation was measured by the incorporation of tritiated methyl groups from radioactively labeled SAM (Perkin Elmer) into the biotinylated substrate using an avidin–biotin methylation plate assay47 ...
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bioRxiv - Plant Biology 2022Quote: ... The membranes were UV cross-linked and hybridized with miRNA probes labeled with [γ-32P] ATP (PerkinElmer), with U6 served as loading control ...
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bioRxiv - Cell Biology 2022Quote: ... and cells were incubated for 5 min before addition of 200μCi 35S-labeled methionine/cysteine mixture (PerkinElmer) and incubation for 1 hr at 37°C ...
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bioRxiv - Immunology 2023Quote: ... cells were labeled with 0.5 uCi of 56 mCi/mmol [14C] acetic acid (Perkin Elmer Cat# NEC084H001MC) per well overnight ...
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bioRxiv - Microbiology 2023Quote: ... The membrane was probed with a goat anti-mouse antibody conjugated to horseradish peroxidase and bands were visualized using the Western Lighting Plus-ECL reagent (Perkin Elmer). When performing the KatG expression analysis in response to C10 treatment ...
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bioRxiv - Molecular Biology 2019Quote: ... 0.5% Sarkosyl, 2.5 mM MgCl2, 0.5 mM DTT, 0.05#mM biotin-A/C/G/UTP [Perkin Elmer], 0.4 u/μl RNase inhibitor). The total RNA was isolated with Trizol LS (Invitrogen) ...
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bioRxiv - Genomics 2020Quote: ... These steps were repeated starting from blocking for the second staining with primary antibody rat anti-CD8a 1:300, secondary antibody goat anti-rat (MP-7444, Vector) and opal 690 (fp1497, PerkinElmer) and the third staining with rat anti-CD45 1:1,000 ...
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bioRxiv - Molecular Biology 2019Quote: ... cells were metabolically labeled for 10 min with 10 μCi EasyTag™ EXPRESS35S Protein Labeling Mix (Perkin Elmer). Total cell lysate was harvested in RIPA buffer (70 ...
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bioRxiv - Immunology 2020Quote: ... The integrated signal density corresponding to labeled antigen or antibody in each LN was calculated using LivingImage (PerkinElmer) or ImageJ software and plotted using GraphPad Prism 8 ...
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bioRxiv - Microbiology 2020Quote: ... Cultures were grown until OD600 ∼ 0.02 and labeled with 50 μCi/ml 32P-orthophosphate (900 mCi/mmol; PerkinElmer). When OD600 reached 0.2-0.4 ...
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bioRxiv - Microbiology 2020Quote: ... was used for hybridization with oligonucleotides 5’-end labeled with 6,000 Ci/mmol γ-32P ATP (Perkin Elmer) using T4 polynucleotide kinase (NEB) ...
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bioRxiv - Biochemistry 2023Quote: ... the beads were suspended in 25 µl of cytoplasmic buffer containing 2 µCi of 3H-labeled SAM (PerkinElmer) and incubated overnight at 32 °C without shaking ...
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bioRxiv - Cell Biology 2023Quote: ... cells were labeled using an 35S Met/Cys labeling mixture at 200 µCi/ml (EasyTag Express35S; Perkin Elmer) for 30 minutes in media that contained drug treatments and that was 20 percent MEF culture medium as above and 80 percent DMEM lacking Met/Cys and with dialyzed FBS ...