Labshake search
Citations for PerkinElmer :
251 - 300 of 466 citations for Mouse Anti Hepatitis C Virus NS4b Antibody 1858 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2021Quote: ... cells were washed and incubated at 37 °C in phosphate buffered saline (PBS) containing 2 μCi/ml [3H]-glycine (1.6 TBq/mmol; PerkinElmer Life Sciences) isotopically diluted to reach 10 μM final glycine concentration ...
-
bioRxiv - Molecular Biology 2020Quote: ... cells were incubated at restrictive temperature (37°C) for 3 h and finally transfer to 384-well CellCarrier plates (PerkinElmer, 6007550) for imaging on an Opera QEHS high-content screening microscope (PerkinElmer) ...
-
Peripheral neuropathy linked mRNA export factor GANP reshapes gene regulation in human motor neuronsbioRxiv - Neuroscience 2021Quote: ... were hybridized for 2 h at 40°C followed by signal amplification and developing the HRP channel with TSA Plus fluorophore Cyanine 3 (1:1500) (NEL744001KT, Perkin Elmer) according to the manual ...
-
bioRxiv - Molecular Biology 2020Quote: ... 1 µCi 3H-SAM and ∼1 µg of enzyme) for 1 hour at 30° C following which they are transferred to FlashPlates (Perkin Elmer) and incubated for 30 min for peptide capture ...
-
bioRxiv - Cell Biology 2019Quote: ... GST-Aq-NF-κB or GST-Aq-NF-κB-ALA C-terminal peptides and 5 μCi [γ-32P] ATP (Perkin Elmer) in kinase reaction buffer (25 mM Tris-HCl ...
-
bioRxiv - Microbiology 2019Quote: The membrane was hybridized overnight at 50°C with DNA oligo probes labeled with [gamma- 32P]-ATP (6000 Ci/mmol) (Perkin Elmer). 20 pmol of the DNA oligo was mixed with 7 μl of [gamma- 32P]-ATP ...
-
bioRxiv - Neuroscience 2020Quote: ... and incubated 10 minutes at 37 °C by addition of 1 μCi/ml of L-[3,4-3H]-Glutamic acid (Perkin Elmer, NET490) and 10µM L-glutamate (Sigma) ...
-
bioRxiv - Microbiology 2020Quote: ... Cells were incubated for 16–18 hours at 37°C before Fluc activity in cell lysates was detected using a plate luminometer (EnSpire 2300 Multilabel reader by Perkin Elmer) and the Bright-Glo Luciferase Assays System (Promega) ...
-
bioRxiv - Neuroscience 2020Quote: ... Imaging was performed at 37°C and 3% CO2 using an Opera Phenix™ high-content confocal microscopy system (Perkin Elmer). Images were acquired at 40x magnification using a 425 nm laser for excitation of CFP and emissions detected at 435-515 nm (CFP ...
-
bioRxiv - Microbiology 2019Quote: ... 100 μL each of sample and Reagent B were combined in each well of a culture plate and the plate was incubated at 60 °C for 10 minutes before reading absorbance at 432 nm on a Wallac 1420 Victor2 multilabel plate reader (Perkin Elmer). A clean plate was used for each assay ...
-
Surveying the landscape of tRNA modifications by combining tRNA sequencing and RNA mass spectrometrybioRxiv - Biochemistry 2019Quote: ... at 42 °C for 30 min followed by hybridization overnight at 42 °C with 4 pmol DNA probes radiolabeled with [γ-32P]ATP (PerkinElmer) and T4 Polynucleotide kinase (New England Biolabs) ...
-
bioRxiv - Microbiology 2021Quote: ... Liquid cultures were incubated for seven days at 29 °C in the dark and the bioluminescence signal was measured daily with an EnVision plate reader (Perkin Elmer) three times for three seconds per well ...
-
bioRxiv - Microbiology 2021Quote: ... 200 µl of the diluted cultures were transferred onto 96-well plate and OD490 at 37 °C was measured at fixed interval using Victor X4 plate reader (Perkin Elmer). For manual growth curve and CFU enumeration ...
-
bioRxiv - Microbiology 2022Quote: ... at 42 °C for 30 min followed by hybridization overnight at 42°C with 4 pmol probes (Table S3) that were radiolabeled using [γ-32P] ATP (PerkinElmer) and T4 Polynucleotide kinase (New England Biolabs) ...
-
bioRxiv - Synthetic Biology 2022Quote: ... 200 μL of these dilutions were then pipetted into opaque 96-well microplates and incubated at 30°C in a Victor X3 multilabel microplate reader (Perkin Elmer) that periodically measured absorbance (OD600 ...
-
bioRxiv - Synthetic Biology 2022Quote: ... cells were incubated with the AAVs for 24 hours at 37°C and assayed with Britelite plus (Perkin Elmer, cat#6066766) following the manufacturer’s protocol.
-
bioRxiv - Neuroscience 2023Quote: ... The choroid plexus was subsequently swiftly rinsed in cold isotope-free HEPES-aCSF containing 2 mM ouabain (4°C) and transferred to a scintillation vial and dissolved in 100 μl Solvable (6NE9100, Perkin Elmer). For the light and dark phase influx experiment ...
-
bioRxiv - Microbiology 2023Quote: Growth of bacterial cultures to study phage infection or toxicity of SAMase was carried at 37°C in a 96-well format using EnSpire Multimode Plate Reader (Perkin Elmer). Overnight bacterial cultures were diluted 100-fold in LB medium with appropriate antibiotics and grown at 37°C in 10 mL of LB ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... The 96-well plate was incubated for 24h at 37 °C in a Victor3 microtiter plate reader (PerkinElmer, Waltham, MA, USA), and absorbance was measured every three minutes ...
-
bioRxiv - Biochemistry 2023Quote: ... Binding reactions were incubated for 2 hours and then terminated by washing the solutions over a PEI-coated GF/C filter using a cell harvester (Perkin Elmer) followed by wash steps using ice-cold buffer [50 mM HEPES ...
-
bioRxiv - Immunology 2023Quote: ... B cells were lysed in water by osmotic pressure and were transferred from 96 well plates to glass fiber filtermat (8×12 Filtermat A, GF/C, 100/pk, #1450-421, Perkin Elmer). The filters were rinsed with water and sealed in plastic bags with a scintillation cocktail (OptiScint® LLT ...
-
bioRxiv - Cancer Biology 2023Quote: ... then each well was incubated for 30 min at 37 °C with 5 μl/well EXPRE35S35S Protein Labeling Mix (PerkinElmer NEG072002MC), after which cells were lysed in RIPA lysis buffer (50 mM Tris-HCl pH 7.5 ...
-
bioRxiv - Neuroscience 2023Quote: ... Bioluminescence was recorded every 30 min in DD at 25°C and 65% humidity using a TopCount Multiplate Reader (Perkin Elmer). An sCMOS camera (DMK33UX178 ...
-
bioRxiv - Biochemistry 2023Quote: ... Filter-bound [3H]VUF15485 was measured by adding 25 µL Microscint-O per well to the dried GF/C-plate and radioactivity was consecutively quantified using the Wallac Microbeta counter (Perkin Elmer). The sigmoidal dose-dependent displacement curves of [3H]VUF15485 binding by unlabelled ligands were analysed using Graphpad Prism 8 ...
-
bioRxiv - Biochemistry 2023Quote: ... Each binding reaction was allowed to reach equilibrium at 30 °C for 5 min prior to fluorescence polarization measurements in a Victor X5 Multilabel Plate Reader (Perkin Elmer). Each Kd measurement was calculated according to our previous work (45) ...
-
bioRxiv - Biochemistry 2023Quote: ... Reactions are incubated at 30 °C for 20 min prior to luminescence measurement for 10 s on the Victor X5 Multilabel Plate Reader (Perkin Elmer) by addition of 75 μl Renilla luciferase substrate (Promega) ...
-
bioRxiv - Biochemistry 2023Quote: ... Incubations were done at room temperature (24°C) for 4h and the end-point fluorescence reading obtained with a multimode microplate reader (Envision or EnSight, Perkin Elmer) equipped with excitation 405 nm and emission 535 nm filters ...
-
bioRxiv - Cell Biology 2023Quote: ... and 1% antibiotic/antimycotic at 37°C for 30 min and then labeled with Express [35S] Labeling Mix (PerkinElmer, NEG072-007) for 15 min ...
-
bioRxiv - Cell Biology 2024Quote: ... Cells were then maintained at constant 37°C for 36 hours before moving to an Opera Phenix Plus (Revvity Perkin Elmer, at the UCSC Chemical Screening Center RRID SCR_021114) ...
-
bioRxiv - Biophysics 2023Quote: ... and their proliferation was recorded at 37 °C with a Nikon Ti inverted microscope using an Ultraview spinning disk confocal system (Perkin Elmer). For determining TEM opening ...
-
bioRxiv - Genomics 2019Quote: ... followed by blocking with antibody diluent/blocker (Perkin Elmer, Waltham, MA) for 30 min ...
-
bioRxiv - Developmental Biology 2019Quote: ... and detected with a tyramine-amplified fluorescent antibody (Perkin Elmer, NEL753001KT).
-
bioRxiv - Neuroscience 2021Quote: ... antibodies followed by fluorescent staining with Tyramide Signal Amplification system (PerkinElmer). After mounting sections in VectaShield ...
-
bioRxiv - Microbiology 2020Quote: ... and acceptor antibodies were conjugated to Alpha Acceptor Beads (PerkinElmer #6760137M).
-
bioRxiv - Cell Biology 2023Quote: ... or peroxidase-conjugated primary antibodies using Western Lightning Plus-ECL (PerkinElmer) and the chemiluminescent system AI680 (GE Healthcare ...
-
bioRxiv - Molecular Biology 2019Quote: ... 0.5% Sarkosyl, 2.5 mM MgCl2, 0.5 mM DTT, 0.05#mM biotin-A/C/G/UTP [Perkin Elmer], 0.4 u/μl RNase inhibitor). The total RNA was isolated with Trizol LS (Invitrogen) ...
-
bioRxiv - Cancer Biology 2021Quote: ... cells were fixed with 2% PFA at RT for 30 min and stained with the Hoechst 33342 dye (1 μg/ml) at 37 °C and 5% CO2 before imaging with the Operetta™ high content imaging system (PerkinElmer Inc.). Forty fields were acquired from each well using appropriate filter sets ...
-
bioRxiv - Cell Biology 2022Quote: PDE activity was measured at 30°C with the two-step method described by [48] using [3H]cGMP (Perkin Elmer, MA, USA). Aliquots of eluted fraction from SEC were incubated in 60 mM HEPES pH 7.2 assay buffer containing 0.1 mM EGTA ...
-
bioRxiv - Neuroscience 2021Quote: Live-cell imaging was performed at 37°C and with 3% CO2 using an Opera Phenix™ high content confocal microscopy system (Perkin Elmer). One hour prior to imaging ...
-
bioRxiv - Immunology 2020Quote: ... The pyroptotic process was monitored at 34°C and 7.5% CO2 using a fully automated spinning disk confocal microscope (PerkinElmer Technologies, OPERA QEHS) with a 40x water immersion objective (Aulner et al. ...
-
bioRxiv - Immunology 2020Quote: ... and allowed to refold at 18°C for 24 h in a Streptavidin-coated scintillation microplate (Flashplate PLUS, Perkin Elmer, Boston, MA). Dissociation was initiated by adding excess unlabeled β2m and placing the microplate in a scintillation counter (TopCount NXT ...
-
bioRxiv - Plant Biology 2020Quote: ... The reaction was incubated for four hours at 37°C and the results were visualised using the LabChip GX Touch 24 (PerkinElmer, Waltham, USA).
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... The membrane mixture was then filtered over a 0.3% PEI pre-treated GF-B/C plate (#6005174, Perkin Elmer, Waltham, MA, USA) with a cell harvester system ...
-
bioRxiv - Immunology 2022Quote: ... and the reaction was continued for 24 hours at 37 °C before FP measurement using a Victor X5 Multilabel plate reader (PerkinElmer, Shelton, CT). DP4.1-Clip (250 nM) ...
-
bioRxiv - Microbiology 2023Quote: ... in 96-well plates in 28°C with shaking and the OD600 measurements were performed hourly using an EnVision Multilabel Reader (Perkin Elmer, USA).
-
bioRxiv - Cell Biology 2022Quote: ... Nascent proteins are labeled during translation reactions of 15-30 min at 32°C in RRL by incorporation of radioactive 35S-methionine (Perkin Elmer, USA). Nascent TA proteins were released from the ribosome with 1 mM puromycin and then incubated with 5% (v/v ...
-
bioRxiv - Immunology 2023Quote: ... Fluorescent signals were monitored in real-time with a microplate reader for 24 h within a tissue culture incubator (37 °C, 5 % CO2) on black OptiPlate 96-well (PerkinElmer, MA, USA) under 20x objective ...
-
bioRxiv - Molecular Biology 2024Quote: ... This RNA unwinding assay was performed at 37 °C in 384-well plates using a Victor Nivo plate reader (PerkinElmer, Basel, Switzerland). The excitation wavelength was set to 640 nM and the emission was measured at 685 nm ...
-
bioRxiv - Immunology 2021Quote: ... with a PBS solution containing 3mg of luciferin/mouse (XenoLight D-Luciferin - K+ Salt, PerkinElmer, cat. 122799) and imaged using an In Vivo Imaging system (IVIS ...
-
bioRxiv - Immunology 2020Quote: ... with a PBS solution containing 3mg of luciferin/mouse (XenoLight D-Luciferin - K+ Salt, PerkinElmer, cat. 122799) and imaged using an IVIS Spectrum In Vivo Imaging system (PerkinElmer ...