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Citations for PerkinElmer :
251 - 300 of 1145 citations for Integrin beta 1 binding protein 1 ITGB1BP1 Antibody Biotin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... Leucine affinities for WT LeuT and mutants displaying WT substrate affinities (A22V, A22S) were determined by saturation binding of [3H]leucine (25 Ci/mmol) (PerkinElmer), with unspecific binding corrected for by the addition of 100 µM unlabelled leucine ...
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bioRxiv - Genomics 2024Quote: ... and the run-on conducted in the presence of 25 μM biotin-11-A/C/G/UTP (Perkin Elmer cat nrs ...
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Vaccinia virus-based vaccines confer protective immunity against SARS-CoV-2 virus in Syrian hamstersbioRxiv - Immunology 2021Quote: ... with HRP goat anti-mouse IgG Ab (31430, 1:20,000) for 1 h and developed using a Western Lightening Enhanced Chemiluminescence kit (PerkinElmer) according to the manufacturer’s protocol.
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bioRxiv - Immunology 2021Quote: ... 20 μl of a 1:1 mix of Western Lightning oxidizing and enhanced luminol reagents (Perkin Elmer Life Sciences) was added to each well ...
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bioRxiv - Microbiology 2022Quote: ... oligonucleotides were 5’-end-labelled with [γ-32P]-ATP (10 mCi ml-1, 3000 Ci mmol-1, Perkin Elmer) with polynucleotide kinase (Thermo Scientific) ...
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bioRxiv - Microbiology 2024Quote: ... seven or eight replicate bottles were filled and spiked with 50 µCi [14C]-bicarbonate (specific activity 56 mCi mmol−1 / 2.072 × 109 Bq mmol−1; PerkinElmer). To three bottles ...
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bioRxiv - Plant Biology 2024Quote: ... Detection was performed by exposition to a (1:1) solution of Western Lightning® Plus-ECL reagents (Perkin Elmer) for 1 minute and chemiluminescence imaging in a ChemiDoc™ Imaging System (BioRad).
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bioRxiv - Cell Biology 2021Quote: ... Horseradish peroxidase (HRP) conjugated secondary antibody was added in order for labelled proteins to be detected by Western Lightning Plus-ECL (PerkinElmer, 203-170071). Primary and secondary antibodies used are listed in Supplementary Table 5.
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bioRxiv - Genetics 2023Quote: ... A sample of cells were stained with 10 µg ml–1 Hoechst plus 1:500 acridine orange/propidium iodide solution and counted on an Opera Phenix confocal microscope (PerkinElmer). A total of 100,000 cells per well were seeded into neural coating medium-filled 96-well plates in 100 µl per well of neural medium comprising Brainphys medium with 1× B27 plus vitamin A ...
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bioRxiv - Neuroscience 2023Quote: ... the membrane was washed three times and Fixer-Developer (1:1) solution from Western Lightning Chemiluminescence Reagent Plus (PerkinElmer, NEL104001EA) enhanced chemiluminescence (ECL ...
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bioRxiv - Cell Biology 2024Quote: ... Fresh mouse cardiac biopsies (1 biopsy ∼1-3 mg/well) were distributed in a 96-well white microplate (OptiPlate-96; PerkinElmer) and incubated or not (basal ...
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bioRxiv - Molecular Biology 2021Quote: ... Plates were read at 450 nm and readings were processed to observe binding patterns of tested molecules (Perkin Elmer, Enspire Alpha). For every experiment ...
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bioRxiv - Biochemistry 2023Quote: MLL1C binding assays (Figure 2C) were performed as above except using Nickel-chelate acceptor beads (10 μg/mL; Perkin Elmer AL108M), streptavidin donor beads (20 μg/mL ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: V2R binding studies were based on time-resolved fluorescence resonance energy transfer (TR- FRET) measurements using Tag-Lite assays (PerkinElmer Revvity) as previously described19,34,51 ...
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bioRxiv - Genomics 2021Quote: ... Aliquots of 10E6 nuclei were subjected to 3-minute nuclear run-on reactions in the presence of Biotin-11-CTP (PerkinElmer) and PRO-seq libraries were constructed in duplicate as described (31) ...
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bioRxiv - Genomics 2022Quote: ... PRO-seq libraries for all replicates were then prepared simultaneously by subjecting one aliquot of 1e7 nuclei/sample to 3-minute nuclear run-on reactions in the presence of Biotin-11-CTP (PerkinElmer) following our previously detailed protocol (24) ...
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bioRxiv - Genomics 2023Quote: The nuclear run-on experiments for PRO-seq were performed in triplicate as described in 47 with the use of a mixture of rNTPs and Biotin-11-CTP (PerkinElmer), and a range of 10-20 million isolated nuclei per sample ...
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bioRxiv - Genomics 2022Quote: ... nuclei were extracted from ~2×107 cells and run-on reactions were carried on at 37°C for 3 minutes using 3μl of each 11-biotin-labelled ribonucleotide stocks (Perkin Elmer). Following real time PCR amplification in the presence of EvaGreen dye as above ...
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bioRxiv - Developmental Biology 2020Quote: ... A buffer containing Cy3 (PerkinElmer, Waltham, 1:50) was used to stain the samples for 40 min.
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bioRxiv - Microbiology 2021Quote: ... and 1 U of AmpliTaq polymerase (Perkin Elmer). The mixture was subjected to 40 cycles of amplification using a DNA thermal cycler 2720 (Perkin Elmer) ...
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bioRxiv - Neuroscience 2020Quote: ... TSA® Plus Cyanine 5 (PerkinElmer, 1:1,000) was used as a secondary fluorophore for C1 probes (Gtpbp2 ...
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bioRxiv - Cancer Biology 2020Quote: ... incubated for 1 min ECL (Perkin Elmer, USA) and the signal was determined with an INTAS ECL Chemocam imager (INTAS Science Imaging ...
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bioRxiv - Biophysics 2022Quote: ... and 1:125 Streptavidin-XL665 (HTRF acceptor, PerkinElmer) in assay buffer (25 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES ...
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bioRxiv - Cell Biology 2021Quote: ... ERK 1/2 Total (PerkinElmer, #ALSU-TERK-A500) and Cofilin Total (PerkinElmer ...
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bioRxiv - Cell Biology 2021Quote: ... TSA® Plus Cyanine 3 (PerkinElmer, 1:1,500) was used as a secondary fluorophore for Hbs1l-C2 probes.
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bioRxiv - Developmental Biology 2022Quote: ... and anti-DNP-HRP (PerkinElmer FP1129; 1:100) were used in combination with DyLight488-tyramide and DyLight680-tyramide ...
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bioRxiv - Biochemistry 2022Quote: ... 1 μM [3H] SAM (82.3 Ci/mmol, PerkinElmer) and various amounts of refolded RNA in 1X binding buffer ...
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bioRxiv - Developmental Biology 2022Quote: ... and Opal 690 (1:1500, PerkinElmer, Cat. FP1497001KT).
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bioRxiv - Biophysics 2024Quote: ... 1 μL γ 32P ATP (Perkin Elmer, NEG502Z250UC), 1 μg PM187B20 in a 20 μL reaction volume of 1x T4 PNK buffer (NEB ...
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The conserved membrane-proximal domain of Sbh1/ Sec61β guides signal peptides into the Sec61 channelbioRxiv - Biochemistry 2024Quote: ... Proteins were labelled for 5 mins (for soluble proteins) or 15 mins (for membrane proteins) with 30 µCi of Express Protein Labelling Mix (Perkin Elmer) per sample at their restrictive temperature ...
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bioRxiv - Biophysics 2020Quote: ... 100 nM hNatB was mixed with a saturating concentration of “MDVF” peptide substrate (500 μM) and varying concentrations (1.95 μM to 1 mM) of acetyl-CoA (14C-labeled, 4 mCi mmol−1; PerkinElmer Life Sciences) for 10-minute reactions ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 µCi 3H-SAM and ∼1 µg of enzyme) for 1 hour at 30° C following which they are transferred to FlashPlates (Perkin Elmer) and incubated for 30 min for peptide capture ...
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bioRxiv - Microbiology 2022Quote: ... an equivalent of 300 ng of Gag p24 was lysed in ELISA lysis buffer containing 1% Triton (Alliance HIV-1 p24 Antigen ELISA kit, Perkin Elmer) and protease inhibitors for 30 min on ice ...
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bioRxiv - Immunology 2022Quote: ... the medium was removed from all wells and cells were lysed by the addition of 100 µl of a 1:1 dilution of BriteLite assay readout solution (Perkin Elmer) and 1xDPBS (Gibco ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were counterstained with DAPI (1 μg ml-1) before imaging with the Opera Phenix High Content Screening System (Perkin Elmer).
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bioRxiv - Molecular Biology 2023Quote: ... Transcription was initiated with addition of 12 pmol rrnBP1 PCR promoter fragment pre-mixed with 1 mM ATP and 4 μCi α-[32P]-CTP (3,000 Ci mmol−1; Perkin Elmer) at 37 °C ...
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bioRxiv - Microbiology 2022Quote: 643nt pre-crRNA was in vitro transcribed with [a-32P]-ATP (10 mCi ml-1, 3000 Ci mmol-1, Perkin Elmer) as described above ...
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bioRxiv - Neuroscience 2024Quote: ... The probes were visualized using Opal 520 and Opal 570 reagent (1:500 and 1:1000, respectively; PerkinElmer, Woodbridge, ON, CA). PHOX2B immunoreactivity was detected using mouse monoclonal PHOX2B antibody (B-11 ...
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bioRxiv - Microbiology 2024Quote: ... 10 ml cultures were grown in the presence of [32P]-phosphate (final concentration of 1 µCi ml-1; Perkin Elmer NEX060001MC) for until OD600 reached ∼1.5-2 ...
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bioRxiv - Microbiology 2024Quote: ... 10 ml cultures were grown in the presence of [1-14C]-acetate (final concentration of 0.2 µCi ml-1; Perkin Elmer NEC084A001MC) until OD600 reached ∼0.5-0.7 ...
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bioRxiv - Microbiology 2024Quote: ... 5 ml cultures were grown in the presence of [32P]-phosphate (final concentration of 1 µCi ml-1; Perkin Elmer NEX060001MC) overnight ...
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bioRxiv - Bioengineering 2024Quote: ... Assays were read out with luciferase substrates 2 d after infection by removing the media from the wells and adding 80 uL of a 1:1 dilution of BriteLite in DPBS (BriteLite Plus, Perkin Elmer). Luminescence values were measured using a microplate reader (BioTek Synergy™ HT or Tecan M200) ...
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bioRxiv - Biophysics 2024Quote: ... A six-point standard curve from 200 ppb to 1 ppb (200, 100, 50, 10, 5, 1) was prepared from elemental standards (Perkin Elmer), plus a 0-ppb blank ...
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bioRxiv - Bioengineering 2024Quote: ... and scanned four times in the 4000 cm-1 to 650 cm-1 range using transmission mode in the FTIR software (PerkinElmer Spectrum). The peaks from the FTIR readings were analyzed via an infrared spectroscopy tables found in the literature [36].
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bioRxiv - Biochemistry 2024Quote: ... NADPH oxidation was monitored in 1-mL quartz cuvettes with a path length of 1 cm in a Lambda 650 UV/VIS spectrometer (Perkin Elmer) with attached water Peltier system set to the appropriate temperature ...
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bioRxiv - Cancer Biology 2024Quote: AlphaScreen assays for measuring protein-protein interactions were performed using EnSpire reader (PerkinElmer). Biotinylated BACH1-BTB was immobilized to streptavidin-coated AlphaScreen donor beads ...
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bioRxiv - Biochemistry 2020Quote: ... Microtiter 384-well Proxiplates for the ThermoFRET stability assay and 384-well Optiplates for association binding experiments were obtained from PerkinElmer (Beaconsfield, UK).
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bioRxiv - Neuroscience 2024Quote: ... and 50 µl of radioligand diluted in binding buffer ([3H]-N-methylspiperone: 0.4 nM final concentration, Perkin Elmer, Waltham, MA; [3H]-(R)-(+)-7-OH-DPAT ...
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bioRxiv - Bioengineering 2023Quote: ... for SARS-CoV-2 S protein neutralization antibody titer in the wells was observed using the High Content Analysis System (Perkin Elmer, Waltham, MA, USA) or microscope ...
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bioRxiv - Cancer Biology 2021Quote: ... For each PI3K isoform a 3 µL mixture (1:1) of enzyme/lipid substrate solution was added to a 384 well white assay plate (Perkin Elmer #6007299) containing 50 µL of test compound or DMSO only for untreated controls ...