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Citations for PerkinElmer :
251 - 300 of 411 citations for ATP citrate lyase Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2021Quote: ... Probes were end-labelled [1 pmol] using 10 μCi of [γ-32P]-ATP (PerkinElmer, Waltham, MA) and 10 U polynucleotide kinase (New England Biolabs ...
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bioRxiv - Molecular Biology 2019Quote: ... DNA probes were 5’-phosphorylated with T4 polynucleotide kinase (PNK, TOYOBO) and [γ-32P] ATP (PerkinElmer). Northern blotting of tRNAs was performed using PerfectHyb (Toyobo ...
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bioRxiv - Cell Biology 2021Quote: ... Phosphate incorporation was visualized by adding 1 µCi 32P-γ-ATP (200 cpm/pmol, Perkin Elmer), plus autoradiography or a PhosphorImager system (Molecular Dynamics) ...
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Identification and biochemical characterization of a novel eukaryotic-like Ser/Thr kinase in E. colibioRxiv - Microbiology 2020Quote: Autophosphorylation of YegI was performed by addition of 5 μCi of [γ-32P] ATP (Perkin Elmer) to different concentrations of purified YegI (0.0625,0.125,0.25,0.5,1,2 μM ...
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Identification and biochemical characterization of a novel eukaryotic-like Ser/Thr kinase in E. colibioRxiv - Microbiology 2020Quote: Autophosphorylation of YegI was performed by addition of 5 μCi of [γ-32P] ATP (Perkin Elmer) to 1 μM of purified YegI in 10 μl of reaction buffer containing 50 mM Tris pH 7.5 ...
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bioRxiv - Molecular Biology 2020Quote: ... 1/20th of the annealed substrates were 5′-end-labeled with [γ-32P]-ATP (Perkin Elmer) and T4 polynucleotide kinase (New England Biolabs) ...
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bioRxiv - Molecular Biology 2021Quote: 1.5 μg RNA probes (5xAAAAUU or 5xGGGGCC) (Dharmacon) were end-radiolabelled with γ[32P]-ATP (PerkinElmer) using T4 polynucleotide kinase (PNK ...
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bioRxiv - Microbiology 2021Quote: ... in buffer A and [γ-P32]-ATP (10 mCi/mL, 3,000 Ci/mmol-Perkin Elmer # BLU002A). The labelled RNA was gel-purified as described above.
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bioRxiv - Molecular Biology 2020Quote: ATP luminescence was determined in triplicates using the ATPlite luminescence assay (Perkin Elmer, Waltham, MA, USA) with a ClarioStar Platereader (BMG ...
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bioRxiv - Microbiology 2022Quote: ... Northern probes were 5’ 32P-end labeled with 0.3 mCi of y-32P ATP (Perkin Elmer) with 10 U of T4 Polynucleotide kinase (NEB ...
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bioRxiv - Evolutionary Biology 2023Quote: ... the kinase was autophosphorylated for 1 h at 30 °C with [γ-32P]ATP (Perkin Elmer) before being combined with RRs at a 1:4 ratio (10-μl reactions contained 1 μM EnvZ and 4 μM RR) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... [3H]-α,β-methylene ATP concentrations were checked on Tri Carb scintillation beta counters (PerkinElmer, USA). Data analysis was conducted using GraphPad Prism v9 ...
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bioRxiv - Cell Biology 2021Quote: ... washed cells were pelleted, resuspended in 226 μl of Digestion Solution (200 μl 1.2 M sorbitol-citrate, 20 μl glusulase (PerkinElmer), 6 μl 10 mg/ml zymolyase (AMS Biotechnology) ...
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bioRxiv - Developmental Biology 2021Quote: Bulk levels of ATP were measured using a luciferase-based biochemical assay (ATPlite Luminescence Assay System, PerkinElmer). Wing discs from third instar upcrawling larvae were dissected in culture medium ...
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bioRxiv - Genomics 2020Quote: ... at 37°C for 1 hr and then labeled with 0.5μl of gamma 32P-ATP (PerkinElmer, BLU502A250UC) using 20U T4 polynucleotide kinase (NEB ...
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bioRxiv - Biochemistry 2021Quote: ... 100 pmol of RNA were 5’-end labeled with [γ- 32P]-ATP (3000 Ci/mmol, Perkin Elmer) using T4 polynucleotide kinase (NEB ...
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bioRxiv - Microbiology 2020Quote: ... and 2.5 μCi of [γ32P]-ATP (stock of 6000 Ci/mmol 10 mCi/mL, from Perkin Elmer). Autophosphorylation reactions were performed at 30 °C ...
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bioRxiv - Physiology 2022Quote: ... and 0.01% Triton X-100) in the presence of 10 μCi [32P]-γ-ATP (Perkin Elmer Company) with a final volume of 20 μl ...
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bioRxiv - Molecular Biology 2022Quote: DNA probes (U1-SH, U4-14B, and U6-SH) were end labeled with [γ-32P]-ATP (PerkinElmer) using T4 PNK (Thermo Scientific ...
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bioRxiv - Molecular Biology 2020Quote: Aptamers (15 pmol) and DNA double stranded oligonucleotides were end-labelled with [γ-32P] ATP (Perkin Elmer) and T4 polynucleotide kinase (New England Biolabs) ...
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bioRxiv - Microbiology 2019Quote: ... was prepared and labeled according to manufacturer’s guidelines using [gamma- 32P]-ATP (6,000 Ci/mmol) (Perkin Elmer) and T4 Polynucleotide Kinase (PNK ...
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bioRxiv - Biophysics 2021Quote: ... The primer was labeled on its 5’end with (γ 32P)-ATP (Perkin Elmer, 3000 Ci.mmol−1) and T4 polynucleotide kinase (Thermofisher) ...
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bioRxiv - Plant Biology 2022Quote: ... The membranes were UV cross-linked and hybridized with miRNA probes labeled with [γ-32P] ATP (PerkinElmer), with U6 served as loading control ...
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bioRxiv - Biochemistry 2024Quote: Radiolabeled 32P-ATP (3000 Ci/mmol) used for synthesis of 32P-cGAMP was purchased from Perkin Elmer. 1 μM purified sscGAS was incubated with 20 mM Tris-HCl pH 7.4 ...
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bioRxiv - Cell Biology 2021Quote: ... buffer and 1.2 M sorbitol citrate solution, and then digested by 1.4 mg/ml 20T zymolyase (amsbio, 120491) and glusulase (PerkinElmer, NEE154001EA). Cells were spread on poly-L-lysine (Sigma ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.5 mM DTT, 0.5 % Sarkosyl, 0.4 u/μl RNase inhibitor, 0.025 mM biotin-ATP/CTP/GTP/UTP [Perkin Elmer]). Equal amounts of nuclei extracted from Drosophila S2 cells were used as spike-in material in run-on reactions ...
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bioRxiv - Biochemistry 2020Quote: ... Labeling reactions were designed to use equimolar amounts of DNA and γ-32P-ATP (Perkin Elmer cat. # NEG035C001MC) and consisted of 37.5 pmol (7.5 μl of 5 μM ...
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bioRxiv - Microbiology 2021Quote: ... of which one was [5’-32P] single-end labelled with (γ 32P)-ATP (Perkin Elmer, 3000 Ci.mmol-1) and T4 polynucleotide kinase (Thermofisher ...
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bioRxiv - Bioengineering 2021Quote: ... ATP concentrations were then measured using 1.0 second luminescence exposure on a Wallac3 Victor plate reader (PerkinElmer, USA). For the live/dead assay ...
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bioRxiv - Microbiology 2020Quote: ... was used for hybridization with oligonucleotides 5’-end labeled with 6,000 Ci/mmol γ-32P ATP (Perkin Elmer) using T4 polynucleotide kinase (NEB) ...
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bioRxiv - Bioengineering 2022Quote: ATP levels in live cells were assessed by bioluminescence on the IVIS Spectrum in vivo imaging system (PerkinElmer) after adding 150 μg/mL of D-luciferin (PerkinElmer) ...
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bioRxiv - Cancer Biology 2023Quote: ... 0.5 mM DTT, 0.5% Sarkosyl, 0.4 U/µl RNase inhibitor, 0.025 mM biotin-ATP/CTP/GTP/UTP [Perkin Elmer]). Total RNA was extracted using Trizol and ethanol precipitation ...
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bioRxiv - Cancer Biology 2020Quote: ... SORE6>GFP sensor expressing MDA-MB-231 primary tumor FFPE sections were treated with 1X citrate pH 6.0 buffer (PerkinElmer, cat# AR6001) and stained with chicken anti-GFP (Novus ...
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bioRxiv - Biochemistry 2019Quote: ... Oligonucleotides were 5’-end-labeled with [γ-32P]-ATP (10 mCi ml-1, 3000 Ci mmol-1; Perkin Elmer) using polynucleotide kinase (Thermo Scientific) ...
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bioRxiv - Microbiology 2019Quote: ... primer (5’-AGUUGUUGAUC) was 32P-labeled at the 5′ position upon incubation with [γ-32P] ATP (PerkinElmer, Waltham, MA) and T4 polynucleotide kinase (NEB ...
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bioRxiv - Physiology 2021Quote: ... immunoprecipitates were analyzed for kinase activity in presence of phosphatidylinositol (PI) and γ-32P-ATP (PerkinElmer, Santa Clara, CA) followed a published method 91 ...
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bioRxiv - Microbiology 2022Quote: ... one ml of culture was labelled for 4 min at 30 °C using 30 μCi γ-[32P]ATP (PerkinElmer). Samples were incubated with 6% sucrose ...
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bioRxiv - Cancer Biology 2022Quote: ... ATP was measured in supernatants according to the protocol described by the ATPlite 1 step Luminescence Assay System (PerkinElmer). Luminescence readings were obtained from a Bioteck Synergy HT plate reader ...
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bioRxiv - Biochemistry 2021Quote: ... The reactions that monitored [32P]AMP formation were supplemented with [α-32P]ATP (0.01-0.1 mCi/mL) (Perkin Elmer) while the reactions that monitored AA-[32P]tRNA were supplemented with [32P]tRNA (0.01-0.1 mCi/mL) ...
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Identification and biochemical characterization of a novel eukaryotic-like Ser/Thr kinase in E. colibioRxiv - Microbiology 2020Quote: Autophosphorylation of YegI and point mutants was performed by addition of 5 μCi of [γ-32P] ATP (Perkin Elmer) to 0.2 μM of purified YegI or YegI point mutants in 10 μl of reaction buffer containing 50 mM Tris pH 7.5 ...
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bioRxiv - Microbiology 2022Quote: ... oligonucleotides were 5’-end-labelled with [γ-32P]-ATP (10 mCi ml-1, 3000 Ci mmol-1, Perkin Elmer) with polynucleotide kinase (Thermo Scientific) ...
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bioRxiv - Microbiology 2022Quote: ... Extracted crRNAs were end-labeled with T4 Polynucleotide Kinase in a reaction containing γ-[32P]-ATP (PerkinElmer, MA, USA), and resolved on an 8% Urea PAGE ...
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bioRxiv - Neuroscience 2023Quote: ... Emitted light corresponding to the number of ATPs was measured in a Victor® XLight Multimode Plate Reader (PerkinElmer). For each sample ...
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bioRxiv - Immunology 2023Quote: ... 5x104 of cells were resuspended in 150ul of phenol-free RPMI media and spiked with 20uM of ATP (PerkinElmer). After 30min of incubation at 37°C supernatants were collected ...
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bioRxiv - Molecular Biology 2023Quote: ... with a total length of 109 ntb was prepared by 5′-end-labelling using [γ-32P]- ATP (Perkin Elmer) and T4 polynucleotide kinase (Thermo Scientific ...
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bioRxiv - Immunology 2023Quote: ... 5x104 of cells were resuspended in 150ul of phenol free RPMI media and spiked with 20uM of ATP (PerkinElmer). After 10min of incubation at 37°C supernatants were collected and concentration of ATP/sample was measured using ATPlite Luminescence Assay System (PerkinElmer ...
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bioRxiv - Neuroscience 2023Quote: ... CaMKII activity was assessed by its ability to transfer a radioactive phosphate from ψ32P-ATP (Perkin Elmer, Shelton, CT) onto the highly selective peptide substrate Autocamtide-2 (amino acid sequence KKALRRQETVDAL ...
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bioRxiv - Biochemistry 2023Quote: ... 10 μM purified IKK2 protein was first autophosphorylated in the presence of 100-200 nM of γ32P-ATP (PerkinElmer) in kinase assay buffer for 1 hour at 30°C ...
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bioRxiv - Molecular Biology 2024Quote: ... from synthetic oligonucleotides (0.2 µM) and trace-labeled with 32P alpha-ATP (800 Ci/mmol, 12.5 µM, Perkin Elmer) in a 20 µl reaction according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... the reactions were started by adding 5× NTP mixture (1 mM for ATP, CTP and GTP, 0.1 mM for UTP) containing 2 µCi of [α−32P] UTP (PerkinElmer). Transcription reactions were performed at 37°C for 20 min ...