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Citations for PerkinElmer :
251 - 300 of 656 citations for 6 Hydroxymethyl 4 methoxy 5 methyl nicotinic acid methyl ester since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... 500nl of 2 µM siRNAs (20nM final concentration) were distributed on 6 different 384-well imaging plates (CellCarrier Ultra, Perkin Elmer), as described in Table 3 ...
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bioRxiv - Immunology 2021Quote: ... brain and testis tissue samples from hACE2 transgenic mice challenged intranasally with SARS-CoV-2-nLuc (1 x 105 FFU; 6 dpi) were imaged after necropsy using bioluminescence imaging (IVIS, Perkin Elmer), pruned to isolate regions with high nLuc activity and immediately pre-fixed with 3 % glutaraldehyde ...
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bioRxiv - Immunology 2021Quote: M-CSF-dependent mouse bone marrow derived macrophages isolated from the long bones of C57BL/6 mice were cultured for 48 hours on devitalized bovine bone discs (0.2 mm x 6 mm) labelled with fluorescently conjugated inert bisphosphonate (Osteosense, Perkin Elmer, NEV10020EX) either in the absence or in the presence of mature osteoclasts that had been differentiated for 5-days under pro-osteoclastogenic conditions i.e ...
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bioRxiv - Cancer Biology 2021Quote: ... sections were stained for NICD1/KRT14/DNA or NICD1/Ki67/DNA and epithelial thickness measured and cell counting performed using Volocity 6 software (Perkin Elmer). Cell density was measured by counting the number of nuclei identified across the width of the epithelium on high resolution images of cryosections stained with DAPI and keratinocyte markers such as KRT4 and ITGA6 and dividing this number by the measured surface area.
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bioRxiv - Cancer Biology 2021Quote: ... Contiguous areas were analyzed for presence or absence of NOTCH1 or nuclear NICD1 staining and their length along the section measured using Volocity 6 software (Perkin Elmer). For morphological analysis of NICD1 and positive negative areas (Extended data Fig.1 f-h) ...
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bioRxiv - Cancer Biology 2021Quote: ... stained sections were imaged using Leica TCD SP8 confocal microscope at 40x objective as described in ‘confocal microscopy’ and analyzed with Volocity 6 software (Perkin Elmer). For counting Ki67 positive basal cells in the aged tissue (Fig.5d) ...
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bioRxiv - Cell Biology 2020Quote: ... subconfluent HDLECs cultured in 12-well plates were incubated with 1 ml per well EBM2 medium (containing appropriate amounts of serum and supplement) with [6-14C]-glucose (Perkin Elmer) for 6 hours ...
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bioRxiv - Developmental Biology 2023Quote: ... Paracellular permeability was assessed by mucosal-to-serosal flux (%flux/cm2/hr) of the small inert probe 51Cr-EDTA (360 Da; 6 μCi/mL) (Perkin Elmer), by taking an initial radiolabelled sample of mucosal buffer and then samples from the serosal compartment every 30 minutes for 2 hours ...
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bioRxiv - Microbiology 2024Quote: ... The filters were rinsed with 35 ‰ sodium chloride (NaCl) solution [50] and transferred into 7 mL scintillation vials containing a 6 mL scintillation cocktail (Ultima Gold ™ Cocktail, PerkinElmer). The radioactivity of the filters was determined using a Tri-Carb 3110TR liquid scintillation counter [51] ...
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bioRxiv - Immunology 2024Quote: ... Pups were injected intraperitoneally with D luciferin (150 mg/kg) at 6 hours after LNP injection and imaged using an IVIS Lumina system (Perkin Elmer). To evaluate the in vivo response to CGT ODNs ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were pulse labeled for 15 minutes with 132 μCi/6 cm dish with EasyTag Express 35S Protein Labeling Mix (Perkin Elmer). Radiolabeling was stopped by adding excess ...
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bioRxiv - Immunology 2022Quote: ... Paracellular permeability was determined by measuring mucosal-to-serosal flux (%flux/cm2/hour) of the small inert probe 51Cr-EDTA (360 Da; 6 μCi/mL) (Perkin Elmer), by taking an initial radiolabelled sample of mucosal buffer and then samples from the serosal compartment every 30 minutes for 2 hours ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... was added to the cell suspension and cells were plated (2,500 cells, 6 μL) in a 384-well white opaque microtiter plate (OptiPlates, Perkin Elmer, 6007299). Cells were incubated with serial dilutions (3 μL ...
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bioRxiv - Synthetic Biology 2020Quote: ... Radioactively labelled protein samples were then precipitated using trichloroacetic acid (TCA) and their radioactive counts measured by liquid scintillation using a MicroBeta2 (PerkinElmer, Waltham, MA) to quantify soluble and total luciferase yields as previously reported (Jewett et al. ...
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bioRxiv - Molecular Biology 2020Quote: ... Ten nanograms of crRNA was 5′-labeled with [γ-32P]-ATP (PerkinElmer) using T4 polynucleotide kinase (New England Biolabs ...
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bioRxiv - Molecular Biology 2021Quote: ... and the probes were labeled by α-32P-deoxycytidine 5’-triphosphate (PerkinElmer) using Random Primer DNA Labeling Kit Ver ...
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bioRxiv - Genomics 2020Quote: ... and labeled with TSA Cyanine 5 (Perkin Elmer #NEL705A001KT, 1:1000 dilution). Next ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... every 5 min for 50 min in a Victor X5 (Perkin Elmer) multimode plate reader ...
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bioRxiv - Neuroscience 2020Quote: ... The RNA was then 5’-end-labeled with γ-(32P) ATP (PerkinElmer) using T4 polynucleotide kinase (New England Biolabs ...
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bioRxiv - Biochemistry 2021Quote: ... The oligos were 5’-end radiolabeled with gamma-[32P]-ATP (Perkin Elmer) using T4 polynuclotide kinase (NEB ...
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bioRxiv - Plant Biology 2020Quote: ... 1 μM cold ATP and 5 μCi [γ32P] - ATP (Perkin Elmer, USA). The reaction was incubated for 30 minutes at 30°C and stopped by adding Laemmli buffer and boiling at 95°C for 5 minutes ...
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bioRxiv - Plant Biology 2021Quote: ... covered with 5 ml of Ultima Gold XR scintillation cocktail (Perkin Elmer), and the radioactivity was counted by using a LS6500 multi-purpose scintillation counter (Beckman) ...
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bioRxiv - Cancer Biology 2021Quote: ... 5 µCi of [1-14C] acetate (NEC084H001MC, Perkin Elmer, Waltham, MA, USA) per mL was added and the cells were cultured for four more hours ...
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bioRxiv - Biochemistry 2020Quote: ... The RNA was subsequently 5’-end labeled with [γ32-P] ATP (PerkinElmer) using T4 polynucleotide kinase (PNK ...
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bioRxiv - Biochemistry 2020Quote: ... Oligonucleotides were radioactively [5’-32P]-labelled using [γ-32P] ATP (Perkin Elmer) using T4 polynucleotide kinase (Fermentas ...
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bioRxiv - Microbiology 2020Quote: [5′-α-32P]-pppGpp was synthesized from [α-32P]-GTP (Perkin Elmer) and ATP using RelSeq (1-385 ...
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bioRxiv - Neuroscience 2020Quote: ... Images acquired at 5× were stitched using the Harmony software (Perkin Elmer) to generate whole brain section images.
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bioRxiv - Biochemistry 2021Quote: The 3xTEL oligo was 5⍰ radiolabeled with [γ-32P]ATP (NEG035C005MC, PerkinElmer) using a standard T4 polynucleotide kinase labeling protocol (M0201L ...
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bioRxiv - Neuroscience 2021Quote: ... and a cyanin-5 substrate kit (CY5, TSA-Plus system, Perkin Elmer). Nuclei were counterstained with 4’ ...
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bioRxiv - Cell Biology 2024Quote: ... 5 µl lysate was added to each 384 well Alpha plates (PerkinElmer) together with 10 µl Tem1 coated donor beads and 10 µl 50 µg/µl AlphaLISA Anti-GFP Acceptor Beads (PerkinElmer AL133C ...
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bioRxiv - Microbiology 2024Quote: Triplicate samples (50 mL) were spiked with 5 µCi NaH14CO3 (NEC086H005MC, PerkinElmer) and incubated for 6 h under dark or ambient light conditions ...
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bioRxiv - Biochemistry 2024Quote: ... The oligonucleotides were 5’-end labelled with [γ-32P]-ATP (PerkinElmer, France) or labelled with fluorescent dye Cy5 (Cyanine5 ...
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bioRxiv - Plant Biology 2024Quote: ... 5 pmol of dephosphorylated RNAs were labeled with 32P-γ-ATP (PerkinElmer) by T4 Polynucleotide Kinase (New England Biolabs) ...
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bioRxiv - Cell Biology 2024Quote: ... buffered with CO2 (5%) with the Opera Phenix™ microscope (Perkin Elmer). The objective used is a x20 water objective ...
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bioRxiv - Plant Biology 2021Quote: ... The product 4-methylumbelliferon (MU) was quantified using a fluorimeter (Perkin Elmer LS55, USA). Protein concentration was determined by Bradford assay (Bio-Rad ...
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bioRxiv - Plant Biology 2021Quote: ... and 125 nCi of [4-14C]isopentenyl pyrophosphate (IPP) (Perkin Elmer, 50.6 mCi/mmol) in deionized water ...
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bioRxiv - Molecular Biology 2022Quote: ... then lysed 4 hours later in 100 uL of SteadyLite Plus luciferase reporter (PerkinElmer). As an internal control ...
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bioRxiv - Molecular Biology 2020Quote: ... and then punched into scintillation vials with 4 ml Ultima Gold scintillant (Perkin Elmer). Radioligand binding was quantified by scintillation counting with a Tri-Carb Liquid Scintillation Analyser (Perkin Elmer ...
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bioRxiv - Cancer Biology 2023Quote: ... slides were incubated with biotinylated tyramide (Perkin Elmer; Opal 4-color Fluorescent IHC kit) diluted at 1:50 in amplification buffer for 10 minutes ...
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bioRxiv - Plant Biology 2024Quote: 4 μg of each protein were incubated with 200 nCi [γ32-P] ATP (PerkinElmer) in 5 mM Tris-HCl pH 8.0 ...
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bioRxiv - Molecular Biology 2020Quote: ... 5 µl of the virus or virus-antibody complex was added to each well in triplicates and allowed to incubate for 6 hrs before the cells were lysed with 50 µl/well of steadylite plus luciferase reagent (Perkin Elmer; 6066751). The luciferase activity was quantified using a BMG PHERAstar FS plate reader.
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bioRxiv - Genetics 2020Quote: ... 50 U of DNA polymerase I (M0209S) and 30 μM of each dNTP including 6 μM of Fluorescein-12-dATP (Perkin Elmer, NEL465001EA) or 6 μM of Texas Red-5-dATP (Perkin Elmer ...
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bioRxiv - Molecular Biology 2024Quote: ... 6 animals/genotype) were used to acquire FTIR spectral imaging data using Spectrum Spotlight 400 FTIR Imaging system (Perkin Elmer, Shelton, CT), operating in the mid-IR region of 4,000 - 850 cm−1 at a spectral resolution of 8 cm−1 and spatial resolution of 25 μm ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... [U-14C] labeled acid-soluble β-oxidation products were measured in a Tri-Carb 4910TR liquid scintillation counter (PerkinElmer, Villebon-Sur-Yvette, France). Results were normalized to total protein content and expressed as count per minute (CPM)/ µg of proteins.
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bioRxiv - Biophysics 2021Quote: ... pH7.4) containing 3nM [3H]5-CT (44-158 Ci/mmol, PerkinElmer, Waltham, MA), serial dilutions of competitor (100μM to 0.01nM) ...
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bioRxiv - Cancer Biology 2020Quote: We incubated 5′-labeled 32P-miR-1236 (0.5 nM; PerkinElmer, Boston, MA, USA) with increasing concentrations of in vitro-transcribed WT or 402C>G FOXL2 mRNAs (0 ...
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bioRxiv - Systems Biology 2022Quote: ... equipped with a climatisation control maintaining 37°C and 5%CO2 (Perkin Elmer). For the duration of 2 hours ...
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bioRxiv - Biochemistry 2020Quote: ... pH 5) on label-free microplates (EnSpire-LFB high sensitivity microplates, Perkin Elmer) was obtained by amine-coupling chemistry ...
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bioRxiv - Genetics 2020Quote: ... DNA oligonucleotide (100 ng) was 5’-end labeled with [γ-P32]-ATP (PerkinElmer) using polynucleotide kinase (New England Biolabs ...
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bioRxiv - Microbiology 2020Quote: ... DNA oligonucleotide probes were 5’ end-labeled with [γ-32P]ATP (PerkinElmer, NEG502A) and T4 polynucleotide kinase (Promega ...