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Citations for PerkinElmer :
251 - 300 of 308 citations for 2 9H Fluoren 2 Yldimethylsilyl Phenyl Methanol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Immuno-complexes were incubated for 20 min at 30°C in 30 μl of kinase buffer with a final concentration of 50 μM ATP and [γ-32P] ATP (1 x 10−2 μCi/pmol; PerkinElmer). Reactions were stopped by adding 5X LB ...
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bioRxiv - Neuroscience 2023Quote: ... The choroid plexus was subsequently swiftly rinsed in cold isotope-free HEPES-aCSF containing 2 mM ouabain (4°C) and transferred to a scintillation vial and dissolved in 100 μl Solvable (6NE9100, Perkin Elmer). For the light and dark phase influx experiment ...
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bioRxiv - Physiology 2023Quote: ... the animals were fasted overnight and a bolus of 2-deoxy-D-[1,2-3H]-glucose (1mC/ml; specific activity: 20Ci/mmol; PerkinElmer, Madrid, Spain) mixed with glucose (100µCi/kg body weight ...
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bioRxiv - Molecular Biology 2023Quote: ... the reaction was initiated by the addition of 3 μl of 2 mM [ψ-32P]ATP (222 TBq/mmol Perkin Elmer) to the mixture (final concentration ...
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bioRxiv - Biochemistry 2023Quote: ... 690 uL of the samples were transferred to a scintillation vial with 2 mL of Insta-Gel Plus scintillation cocktail (Perkin Elmer). Radioactivity was measured with the Scintillation counter (Perkin Elmer) ...
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bioRxiv - Microbiology 2024Quote: ... Sample volumes were then normalized to 2 mL and determined by inductively coupled plasma mass spectrometry (ICP-MS) with a NexION 300X instrument (PerkinElmer, USA). To detect intracellular iron ...
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bioRxiv - Neuroscience 2024Quote: ... The supernatant and precipitate were separated and both fractions were counted in a gamma counter (Wizard 2 2480, Perkin Elmer, USA). Following this ...
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bioRxiv - Microbiology 2024Quote: ... were reactions (final volume of 200 μl) were performed in microtiter plates and recorded with a Victor-2 plate reader (Perkin Elmer) Every experiment was done with two biological samples and three technical replicates.
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bioRxiv - Cell Biology 2024Quote: Hep G2 and U-2 OS cells are counted and seeded into 384 well plates (PhenoPlate 384-well, Perkin Elmer, 6057328) using a Biotek or Multidrop microplate dispenser ...
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bioRxiv - Physiology 2024Quote: Metabolic flux assessment using the Seahorse XFe analyser and 2-[1,2-3H(N)]-deoxy-D-glucose (3H-2DOG; Perkin Elmer, USA) uptake assays were performed on scramble and siPKD1/2/3 transfected 3T3-L1 differentiated adipocytes five days post-differentiation in 24-well plates ...
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bioRxiv - Bioengineering 2020Quote: ... In vivo and ex vivo fluorescence imaging was carried out using a planar (2-D) IVIS Spectrum device (PerkinElmer, Waltham, MA, USA). Pymol (1.7 ...
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bioRxiv - Neuroscience 2020Quote: ... were harvested in Schneider’s medium on ice and transferred to Schneider’s medium containing 35S-methionine/cysteine (2 µCi/µl; Perkin Elmer Labs Inc., NEG709A) and 30 µM chloroquine (to inhibit autophagy) ...
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bioRxiv - Cell Biology 2021Quote: ... Then it was mixed with reaction B which contain 5 μg of the GST-substrate in 5x Kinase buffer containing 2 μCi γ-32P-ATP (PerkinElmer, BLU002Z250UC) in 10-μl reactions ...
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bioRxiv - Cell Biology 2021Quote: RAW cells were incubated for 24 h with inositol-free DMEM (MP Biomedica, CA) containing 10 μCi/ml myo-[2-3H(N)] inositol (Perkin Elmer, MA), 1X insulin-transferrin-selenium-ethanolamine (Gibco) ...
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bioRxiv - Immunology 2021Quote: ... Virus-mAb/plasma-containing media was then aspirated from cells and 100 μL of a 1:2 dilution of SteadyLite Plus (Perkin Elmer, 6066759) in PBS with Ca++ and Mg++ was added to cells ...
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bioRxiv - Microbiology 2021Quote: ... 200 μL of each phase were collected and measured in a 2480 automatic Gamma counter Wizard 2 3” (PerkinElmer, Waltham, MA, USA). LogD value were calculated using Excel by dividing measured values of octanol by water and logarithmize the result ...
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bioRxiv - Microbiology 2021Quote: In all our experiments photon emission of the luminescent bacteria was recorded at regular intervals under general anesthesia (isoflurane, inspired fraction=2%) using IVIS Spectrum and analyzed with Living Image software (PerkinElmer, Richmond, CA) as previously described (Debarbieux et al. ...
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bioRxiv - Immunology 2021Quote: ... Virus-mAb/plasma-containing media was then aspirated from cells and 100 μL of a 1:2 dilution of SteadyLite Plus (Perkin Elmer, 6066759) in PBS with Ca++ and Mg++ was added to cells ...
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bioRxiv - Developmental Biology 2021Quote: ... the RNAscope Multiplex Fluorescent Reagent Kit (v.2) and TSA Plus reagents (Perkin Elmer; 1:500 dilution for Cy3 and Cy5 dyes) or Opal dyes (Akoya Biosciences ...
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bioRxiv - Microbiology 2021Quote: ... Virus-mAb-containing media was then aspirated from cells and 50 μL of a 1:2 dilution of SteadyLite Plus (Perkin Elmer, 6066759) in PBS with Ca++ and Mg++ was added to cells ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Luciferase activity was measured for 2 seconds in intact living cells using a microBeta Wallac luminometer (Perkin Elmer, Villebon sur Yvette, France). Tests were performed in quadruplicate in at least three independent experiments and data were expressed as mean ± s.e.m ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The OptiPlate-384 plate was incubated at room temperature in a Biosolutions AmbiStore™ D (HighRes) for 2 hours before the HTRF signal was measured on an EnVision® HTS plate reader (PerkinElmer). For OSM-induced CCL2 assay in hit validation step ...
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bioRxiv - Biochemistry 2022Quote: ... Detection reagents were incubated with reaction time points for 2 hours at room temperature in 384 well white microplates (PerkinElmer OptiPlate-384) then TR-FRET emission at 665 nm and 615 nm by 320 nm excitation with 50 μs delay and 100 μs integration time was measured using a Molecular Devices SpectraMax M5e plate reader ...
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bioRxiv - Physiology 2024Quote: ... Measurement of radiolabeled 2-deoxyglucose was carried out in a Tri-Carb 4910 TR liquid scintillation analyzer (Perkin Elmer, Waltham, MA, USA) by adding 200 μL of muscle lysate to 4 mL scintillation fluid (Perkin Elmer ...
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bioRxiv - Developmental Biology 2024Quote: ... the University of Liverpool’s centre of preclinical imaging provided the imaging services using the Quantum GX-2 system (PerkinElmer, Inc. Waltham, MA). Whole-body scan images were acquired with the protocol FOV72 ...
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bioRxiv - Biochemistry 2024Quote: ... whereafter lysed cells were added to 2 mL scintillation fluid and radioactivity was measured using a scintillation counter (Perkin Elmer 2450 Microbeta2). Data were analyzed using GraphPad Prism 8.0.0 and fitted to a four-parameter logistic curve from where the IC50 and the 95% confidence interval were calculated ...
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bioRxiv - Cell Biology 2023Quote: ... Plates were shaking at 999 rpm and 28°C and growth was measured by monitoring OD600 every 2 h for 10 h using a plate reader (2300 EnSpire™, Perkin Elmer).
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bioRxiv - Molecular Biology 2023Quote: ... and the amount of 2-NBDG was measured by determining the fluorescence intensities at Ex/Em = 475/550 nm using an ARVO X2 (PerkinElmer Co., Ltd.). To investigate the effect of β-hydroxybutyrate on glucose uptake ...
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bioRxiv - Cancer Biology 2024Quote: ... and the plates incubated for 10 min prior to luminescent detection in a Victor 2 microplate reader (Perkin Elmer, Waltham, MA, USA) and the signal was quantified at 595 nm ...
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bioRxiv - Microbiology 2024Quote: ... RNA transcripts from the ffh and zapA promoters were labeled with 2– 10 µCi of [α-32P] ATP or [α-32P] UTP (PerkinElmer), respectively ...
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bioRxiv - Cancer Biology 2021Quote: Bioluminescence Imaging and Quantification: Mice were anesthetized with isoflurane (2-3% in 100% oxygen) and bioluminescence images acquired with an IVIS Spectrum (Perkin Elmer, Waltham, MA) 5 minutes after intraperitoneal injection of Potassium D-Luciferin solution (Perkin Elmer ...
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bioRxiv - Immunology 2021Quote: ... were plated in 3 ml of complete medium supplemented with IL-2 (20 IU/ml) and incubated with 1 μl of 3H-labeled ROL (PerkinElmer, Boston, MA, USA) for 4 h ...
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bioRxiv - Neuroscience 2020Quote: ... Reaction termination and detection were performed using 1X LANCE detection buffer with 20 mM EDTA and 2 nM LANCE Ultra Europium anti-phospho Glycogen Synthase (Ser641) (Perkin Elmer #TRF0220-M).
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bioRxiv - Physiology 2021Quote: ... were plated per well in 96-well white microplates and 100 nM of GnRH (or vehicle) were injected using Synergy 2 Multi-Mode Microplate Reader (Bio Tek) or Victor X light plate reader (Perkin Elmer Life Sciences). Luminescence signals were recorded for 30 seconds ...
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Sequential dynein effectors regulate axonal autophagosome motility in a maturation-dependent pathwaybioRxiv - Cell Biology 2020Quote: ... All the videos (2 min, 4 frames s-1) were acquired at 37 °C using a Nikon TIRF microscopy system (Perkin Elmer, Waltham, MA) on an inverted Ti microscope equipped with a 100× objective and an ImageEM C9100-13 camera (Hamamatsu Photonics ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... After 6-24 h at 37 °C (as specified in each figure legend) the cell medium was aspirated and substituted with serum-free cell medium containing [3H]thymidine (2 μCi/ml, Perkin Elmer, Waltham, MA). The incubation was continued for 30 min at 37 °C ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... lung tissues harvested from euthanized mice were soaked for 10 min in pre-warmed (37°C) PBS with 2% FBS and a 1:10 dilution of Xenolight RediJect Coelenterazine h (Cat# 706506, Perkin Elmer, Waltham, MA). Following a 12-15 min incubation ...
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bioRxiv - Immunology 2024Quote: ... After an additional 2 hours blood was collected and serum FITC-dextran levels were measured with a Victor V multiparameter plate reader (Perkin Elmer, Waltham, MA). Quantification of serum FITC-dextran levels was performed with the WorkOut 2.5 software by extrapolation against know concentrations of FITC-dextran standard.
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bioRxiv - Cell Biology 2023Quote: All the videos (2 min, 4-5 frames s-1) were acquired at 37°C using a Nikon TIRF microscopy system (Perkin Elmer, Waltham, MA) on an inverted Ti microscope equipped with a 100× objective and an ImageEM C9100-13 camera (Hamamatsu Photonics ...
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bioRxiv - Cancer Biology 2023Quote: ... and the plate was incubated in the dark for 2 h and then read in an EnSpire Alpha multimode plate reader (PerkinElmer, Waltham, MA, USA).
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bioRxiv - Neuroscience 2023Quote: ... the same cell samples were further lysed and digested with 2% HNO3 for intracellular iron content quantification using an ICP-AES instrument (Perkin Elmer Optima 8000).
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bioRxiv - Plant Biology 2024Quote: ... five seedlings were grouped into one scintillation vial containing 2 ml Ultima Gold liquid scintillation cocktail and counted in a Tri-Carb 4910TR Liquid Scintillation counter (Perkin Elmer, Rodgau, Germany).
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bioRxiv - Biochemistry 2024Quote: ... The lysed cells were diluted 1:2 with assay buffer after which 10 μL/well was pipetted to a white 384-well OptiPlate (PerkinElmer, Waltham, MA, USA). Next ...
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bioRxiv - Cell Biology 2020Quote: ... mice were anesthetized with 2% isoflurane and renilla bioluminescence was visualized/quantified by the IVIS Spectrum In Vivo Imaging System (PerkinElmer, 5-minute medium binning) in CDP-UMCG ...
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bioRxiv - Cell Biology 2020Quote: ... the mice were anesthetized with 2% isoflurane and firefly bioluminescence was visualized/quantified using the IVIS Spectrum In Vivo Imaging System (PerkinElmer, 5-minute medium binning) in CDP-UMCG ...
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bioRxiv - Immunology 2021Quote: ... Plasma 2-[1-14C]-Deoxy-D-glucose radioactivity was directly measured in a liquid scintillation counter (Tri-Carb 2810 TR, Perkin Elmer, Waltham, MA, USA). Tissue lysates were processed through ion exchange chromatography columns (Bio Rad Laboratories Inc. ...
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bioRxiv - Plant Biology 2020Quote: ... 5 to 10 mg powder were mixed with 2 ml scintillation cocktail and counts per minute (cpm) recorded with a TRI-Carb 2810TR liquid scintillation analyzer (Perkin Elmer, Waltham, MA, USA).
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bioRxiv - Biochemistry 2021Quote: ... Plates were incubated at room temperature for 2 h before measurement of the fluorescence using an EnVision Multimode Plate (Reader PerkinElmer Life and Analytical Sciences). All values were converted to cAMP concentration using cAMP standard curve performed parallel and data were subsequently normalized to the response of 100 μM forskolin in each cell line ...
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bioRxiv - Bioengineering 2023Quote: ... for SARS-CoV-2 S protein neutralization antibody titer in the wells was observed using the High Content Analysis System (Perkin Elmer, Waltham, MA, USA) or microscope ...
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bioRxiv - Genomics 2023Quote: ... was measured in triplicate and averaged at 405 nm for 45-60 minutes at 25°C on a Wallac Victor 2 1420 Multilabel Counter Plate Reader (Perkin Elmer, Waltham, MA, USA). Citrate synthase activity was standardized against the protein concentration of each sample using the Bovine Serum Albumin Assay (500-0201 ...