Labshake search
Citations for PerkinElmer :
201 - 250 of 391 citations for Transmembrane protein 88 TMEM88 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2021Quote: ... Media were replaced with fresh media supplemented with Easytag Express S35 Protein Labeling Mix (PerkinElmer, 100-200 µCi/mL) for 1 h ...
-
bioRxiv - Neuroscience 2022Quote: ... The images of the targeting proteins were analyzed by a high content screening system (Operetta Enspire, Perkin Elmer, USA). The integral optical density (IOD ...
-
bioRxiv - Microbiology 2022Quote: ... The membranes were incubated with primary and secondary HRP-conjugated antibodies in PBST (5% milk and 0.1% Tween-20) to detect the protein of interest by Western Lightning Plus ECL (PerkinElmer) and ImageQuant LAS 4000 imaging system (GE) ...
-
bioRxiv - Cell Biology 2021Quote: ... cells were metabolically labelled for 20 min with 55 μ Ci/ml 35S- methionine/cysteine protein labelling mix (PerkinElmer). Cells were washed with PBS and cultured in DMEM (Invitrogen ...
-
bioRxiv - Neuroscience 2021Quote: The level of MMP-9 in plasma was measured with an AlphaLISA kit for the human protein (#AL3138, PerkinElmer). AlphaLISA signals were measured using an Ensight Multimode Plate Reader (PerkinElmer).
-
bioRxiv - Immunology 2021Quote: ... The SARS-CoV-2 spike protein antigen (2.0µg/mL) was directly adsorbed on half-area 96-well OptiPlate (96W HB, Perkin Elmer) microplates for 2h at 37°C in a humidified incubator.
-
bioRxiv - Immunology 2020Quote: ... The cpms of immunoprecipitated protein was quantified using a 96-well Microbeta Trilux liquid scintillation plate reader (Perkin Elmer).
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... We next added 0.8 μl of FLAG-GST-transcription factor proteins to 384-well AlphaPlates using a NanoHead (PerkinElmer) and a Janus Workstation (PerkinElmer) ...
-
bioRxiv - Biochemistry 2021Quote: ... 20 μL of protein mixture was added to each well of a 1/2 area 96-well OptiPlate (PerkinElmer). Compounds were then added from DMSO stock to protein mixture (0.4 μl/well ...
-
bioRxiv - Biochemistry 2021Quote: ... 20 μL of protein mixture was added to each well of a 1/2 area 96-well OptiPlate (PerkinElmer). FKBP12F36V competitor or p300-BRD competitor was then added from DMSO stock to protein mixture (0.4 μl/well ...
-
bioRxiv - Microbiology 2021Quote: ... was used (at 1:20.000 dilution) for protein visualization in the presence of Western Lightning Plus-ECL enhanced chemiluminescence substrate (PerkinElmer). The WesternSure® Pre-stained Chemiluminescent Protein Ladder (Li-cor ...
-
bioRxiv - Microbiology 2022Quote: ... cells were metabolically labeled for 16 h with 100 μCi/mL [35S] methionine-cysteine ([35S] protein labeling mix; PerkinElmer) in Dulbecco’s modified Eagle’s medium lacking methionine and cysteine and supplemented with 10% of dialyzed fetal bovine serum and 1X GlutaMAX™ (ThermoFisher Scientific) ...
-
bioRxiv - Biochemistry 2022Quote: ... Cells then were radiolabeled for 15 minutes with 143 µCi/dish EasytagTM 35S Express Protein Labeling Mix (Perkin Elmer) and chased for indicated times in DMEM supplemented with 5 mM unlabeled methionine and cysteine ...
-
bioRxiv - Physiology 2022Quote: ... 60-100 µg protein was incubated with 100 μl TG substrate (25 nmol triolein/assay and 40,000 cpm/nmol 14C-triolein; PerkinElmer) and 35.5 μg mixed micelles of phosphatidylcholine and phosphatidylinositol (3:1 ...
-
bioRxiv - Neuroscience 2022Quote: GPCR activation assays were performed using CHO-K1 cells stably expressing the luminescent Ca2+ indicator aequorin and the promiscuous Gα16 protein (ES-000-A24 cell line, PerkinElmer). The cells were transfected with a dmsr-7/pcDNA3.1 plasmid using lipofectamine LTX and Plus reagent (Invitrogen ...
-
bioRxiv - Cell Biology 2023Quote: ... aliquots of derivatized lysates containing 15 µg of protein were spotted on a Whatman Protran nitrocellulose membrane (Perkin Elmer). Blots were blocked as above ...
-
bioRxiv - Biochemistry 2023Quote: ... 10 μM purified IKK2 protein was first autophosphorylated in the presence of 100-200 nM of γ32P-ATP (PerkinElmer) in kinase assay buffer for 1 hour at 30°C ...
-
bioRxiv - Neuroscience 2024Quote: ... ± drug for 30 minutes then slices were metabolically labelled in 35S-Methionine/Cysteine express protein labeling mix (Perkin Elmer) ± drug for 30 minutes ...
-
bioRxiv - Molecular Biology 2024Quote: ... after 20 min proteins newly synthetized in the mitochondrial compartment were labeled with 100 μCi [35S]-methionine (Perkin Elmer) for 1 h at 37 °C ...
-
bioRxiv - Biochemistry 2021Quote: ... and then incubated with anti-FLAG antibody conjugated to APC (αFLAG-APC; PerkinElmer) diluted 1:100 in PBS + 0.1% BSA at a ratio of 30 μL labeled antibody:107 cells ...
-
bioRxiv - Neuroscience 2022Quote: ... Antibody binding was detected using the enhanced chemiluminescence immunoblotting detection system (Perkin Elmer LLC WESTERN LIGHTNING PLUS-ECL ...
-
bioRxiv - Cell Biology 2022Quote: ... Bound antibodies were detected with chemiluminescent substrate (Western Lightning® Plus, Perkin Elmer) and visualized with iBrightTM CL1000 (Thermo Fisher Scientific).
-
bioRxiv - Immunology 2020Quote: ... The antibodies were visualized by incubation with Tyramide Red or Green (Perkin Elmer) for 10 minutes ...
-
bioRxiv - Genetics 2021Quote: ... horseradish peroxidase-conjugated secondary antibodies and Western Lightining® Plus-ECL (NEL105001EA, PerkinElmer) were used.
-
bioRxiv - Cancer Biology 2022Quote: ... horseradish peroxidase-conjugated secondary antibodies and Western Lightining® Plus-ECL (NEL105001EA, PerkinElmer) were used ...
-
bioRxiv - Immunology 2022Quote: ... Unspecific antibody binding on sorted cells was blocked with TSA blocking reagent (PerkinElmer) for 30 minutes ...
-
bioRxiv - Neuroscience 2024Quote: ... primary antibodies followed by HRP-conjugated secondary polymer (anti-rabbit, anti-mouse [PerkinElmer]) and HRP-reactive OPAL fluorescent reagents (PerkinElmer) ...
-
bioRxiv - Biochemistry 2024Quote: ... Acceptor mixtures were prepared by mixing ULight alpha-6xHIS acceptor antibody (Perkin Elmer) with 6xHIS-tagged Swc5 variants at a ratio of 1:20 and serially diluting across 13 concentrations in H66 buffer (20 mM HEPES pH 7.5 ...
-
bioRxiv - Cell Biology 2023Quote: ... Membranes were washed with TBST and incubated with secondary antibody (NEF812001EA, NEF822001EA, PerkinElmer) for 1 h ...
-
bioRxiv - Cell Biology 2023Quote: ... HRP-conjugated antibodies were detected using western lighting plus-ECL reagent (Perkin elmer).
-
bioRxiv - Neuroscience 2023Quote: ... membranes were incubated with goat anti-rabbit HRP- labelled antibody (Perkin Elmer, # NEF812001EA) at 1/10000 in 1 % (w/v ...
-
bioRxiv - Cancer Biology 2021Quote: ... 30 ug of protein lysate was resolved by SDS-PAGE and transferred onto a Protran pure 0.2 micron nitrocellulose (Perkin Elmer). The membranes were incubated for 1h in 5% skimmed milk and probed with specific primary antibodies (Table S1 ...
-
bioRxiv - Cell Biology 2020Quote: ... and labeled for 5 min with with 1 μL per OD of cells of EXPRESS 35S Protein Labeling Mix (PerkinElmer) for 5 or 10 min ...
-
bioRxiv - Immunology 2021Quote: ... the unbound proteins were washed and luciferase activity was measured using Nano-Glo substrate (Nanolight) in an Envision 2103 microplate luminometer (PerkinElmer).
-
bioRxiv - Genetics 2021Quote: ... Grain protein content (%) and grain yield (t/ha) was collected using a Perten DA 700 NIR analyzer (Perkin Elmer, Sweden) and Wintersteiger Nursery Master combine (Ried im Innkreis ...
-
bioRxiv - Molecular Biology 2020Quote: ... The protein pellet was then re-suspended in PBS and incubated in 4 mL of scintillation fluid (Insta-Gel Plus, PerkinElmer) for 24 h (to homogenize the samples ...
-
bioRxiv - Microbiology 2021Quote: ... 1.37 μg/mL of RBD and 5 μg/mL of Ecto) and incubated overnight in protein-binding 96-well plates (PerkinElmer). The next day ...
-
bioRxiv - Biochemistry 2020Quote: ... newly synthesized proteins in the cells were labeled by treating with 150 μCi/ml of 35S-methionine/cysteine (EXPRE35S35S Protein Labeling Mix, NEG772, PerkinElmer) apically for 45 min (pulse labeling) ...
-
bioRxiv - Biochemistry 2021Quote: ... Cells were pulse-labeled for the indicated times with 143 µCi/dish EasytagTM 35S Express Protein Labeling Mix (Perkin Elmer). After the pulse ...
-
bioRxiv - Neuroscience 2020Quote: ... 3 and 6 hr later cells were labeled for 5 min with 1 mCi/ml 35S Protein Labeling Mix (PerkinElmer). Lysates from 25-ml culture triplicate samples were analyzed by SDS-PAGE and autoradiography.
-
bioRxiv - Microbiology 2020Quote: ... MOLT-4/CCR5 cells were added on day 2 and culture supernatants were examined for the p24 viral capsid protein by ELISA (PerkinElmer) after 14 ...
-
bioRxiv - Neuroscience 2022Quote: ... The samples were incubated for 30 min at 37 °C in the presence of the EasyTag Express Protein Labeling Mix 35S (PerkinElmer), pelleted ...
-
bioRxiv - Microbiology 2020Quote: ... 200 μCi (7.4 MBq) [35S]-methionine/L-[35S] cysteine using EasyTag™ EXPRESS35S Protein Labeling Mix (PerkinElmer, Groningen, the Netherlands) was added to the medium ...
-
bioRxiv - Synthetic Biology 2020Quote: ... Radioactive counts from TCA-precipitated samples was measured by liquid scintillation to then quantify soluble and total yields of each protein produced as previously reported (MicroBeta2; PerkinElmer).27,30
-
bioRxiv - Immunology 2021Quote: ... The excitation and emission wavelengths for each protein were determined prior to the stopped-flow experiments using an LS55 luminescence spectrophotometer (PerkinElmer). The oxidant concentration range required to lead to fluorescence changes for each protein was also determined.
-
bioRxiv - Immunology 2021Quote: ... The proteins were then separated with SDS-PAGE and detected by phosphor imaging (Cyclone Plus storage phosphor system, Perkin Elmer).
-
bioRxiv - Cancer Biology 2022Quote: ... 15 or 10-20% SDS-PAGE gels and proteins were transferred onto PVDF membranes (0.45 μm, Perkin Elmer, Waltham, MA) and blocked with 5% BSA/TBS-T for 2 hours at room temperature ...
-
bioRxiv - Plant Biology 2024Quote: ... Proteins were resolved by 12% SDS-PAGE and dried gels were exposed to a Cyclone Plus Storage Phosphor Screen (PerkinElmer). The screen was scanned using a Typhoon TRIO Variable Mode Imager (GE Healthcare).
-
bioRxiv - Neuroscience 2024Quote: ... 100 µL of cell homogenate (0.05 mg protein/well) and 100 µL of [3H]8-OH-DPAT (0.5 nM final concentration, 170 Ci/mmol, Perkin Elmer) in a final volume of 1 mL ...
-
bioRxiv - Cancer Biology 2023Quote: ... Each protein (5 µL) was mixed and incubated for 1 h in a 384-well AlphaPlate (PerkinElmer, Waltham, MA, USA). After diluting Alpha Screen Protein A Acceptor Beads (PerkinElmer ...