Labshake search
Citations for PerkinElmer :
201 - 250 of 255 citations for Strat M Membrane Transdermal Diffusion Test Model since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2024Quote: ... cell membranes (30 μg protein/tube) were prepared and incubated with 0.05 nM of [35S]GTPγS (PerkinElmer) and varying concentrations (10−11 - 10−5 M ...
-
bioRxiv - Biochemistry 2024Quote: ... the clarified mixture was filtered through a 0.22 µm membrane and analyzed using ICP-MS (NexlON, PerkinElmer). The structures of other GAGases were predicted using RoseTTAfold (Baek ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... The cells were lysed with NaOH (1 M, 1 mL) and transferred to RIA tubes for counting in a γ-counter (Perkin Elmer, Wallac Wizard 1480). The uptake and internalized fraction of the radiotracers were expressed as the percentage of total added activity to each well.
-
bioRxiv - Biochemistry 2021Quote: Eluted protein was separated on an 8% polyacrylamide gel and transferred onto PolyScreen PVDF transfer membrane (Perkin Elmer). Membranes were blocked overnight in TBS containing 5% Bovine Serum Albumin (Sigma Aldrich) ...
-
bioRxiv - Neuroscience 2022Quote: ... 25-30 μg of membrane protein was combined with a fixed concentration (0.57-5.18 nM) of 3H-diprenorphine (PerkinElmer) and concentration curves of KUNB106 or positive control (naloxone for mu and delta ...
-
Adaptive translational pausing is a hallmark of the cellular response to severe environmental stressbioRxiv - Molecular Biology 2020Quote: ... GENIE® blotter (Idea Scientific, 4003) was used to transfer to GeneScreen Plus Hybridization Transfer Membrane (PerkinElmer, NEF986001PK) for 30 min in 1X TBE buffer (0.089 M Tris Base ...
-
bioRxiv - Neuroscience 2021Quote: ... The membranes were treated with the Western Lightning Plus ECL detection reagent (PerkinElmer Life Sciences, catalog no. NEL103001EA) or Clarity Western ECL substrate (Bio-Rad) ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... 30-40 μg of membrane protein was incubated with varying concentrations of compound and 0.3 nM [3H]-CP55,940 (PerkinElmer) for 90 min at room temperature ...
-
bioRxiv - Cell Biology 2020Quote: ... Extracts (15 μg protein) were electrophoresed on SDS-PAGE and then transferred to polyvinylidene difluoride membranes (Perkin Elmer). The membranes were incubated overnight at 40C on rocker with primary antibodies in 5% milk dissolved in 1x PBS tween ...
-
bioRxiv - Cancer Biology 2023Quote: ... a breathable membrane was applied to the plate and cells were imaged on the Operetta HCS microscope (PerkinElmer) at 37 °C ...
-
bioRxiv - Molecular Biology 2022Quote: ... Membranes were washed three times in TBST prior to incubation in ECL solution (Western Lightning Plus, Chemiluminescent Substrate, PerkinElmer) for 5 min and membrane development.
-
bioRxiv - Cell Biology 2020Quote: ... The membranes were then developed with the ECL Plus™ Western blotting detection system (PerkinElmer Life Sciences, Massachusetts, USA).
-
bioRxiv - Biochemistry 2021Quote: ... The amount of bound [35S]GTPγS was quantitated by assaying the membrane with a liquid scintillation counter (Tri-Carb 2910 TR; PerkinElmer).
-
bioRxiv - Cell Biology 2022Quote: ... After thorough wash with TBST for 30 min membranes were treated with Lightning™ Plus Chemiluminescence Reagent (PerkinElmer, NEL104001EA) after mixing the Enhanced Luminol Reagent and the Oxidizing Reagent 1:1 ...
-
bioRxiv - Cell Biology 2022Quote: ... The membranes were then rinsed in TBST 3 times and treated with Western Lightning Plus ECL reagent (PerkinElmer, NEL105001EA). Membranes were exposed to GE Healthcare Hyperfilm ECL (28906839 ...
-
bioRxiv - Cell Biology 2023Quote: ... aliquots of derivatized lysates containing 15 µg of protein were spotted on a Whatman Protran nitrocellulose membrane (Perkin Elmer). Blots were blocked as above ...
-
bioRxiv - Cancer Biology 2023Quote: ... and the membrane was developed using ECL substrate (Western Lightning™ Ultra, Perkin Elmer or Clarity Western ECL Substrate) and scanned using Fusion FX Vilber Lourmat scanner ...
-
bioRxiv - Immunology 2024Quote: ... Clone 2C.4 was screened against human cardiac myosin and the β-adrenergic receptor (βAR) enriched membranes (Perkin Elmer) by ELISA ...
-
bioRxiv - Cell Biology 2022Quote: ... The membranes were washed in TBST at least 3 times and probed with Western Lightning Plus ECL reagent (PerkinElmer, NEL105001EA) and exposed to GE Healthcare Hyperfilm ECL (28906839).
-
bioRxiv - Biophysics 2021Quote: ... GTP loading was initiated by addition of a premixture of cell membranes and WGA-SPA PVT beads (PerkinElmer, Waltham, MA) to a final concentration of 0.25mg beads/well and 15,000cpm 5-HT5AR-Gi1/well (as determined by [3H]-LSD binding) ...
-
bioRxiv - Neuroscience 2020Quote: ... The membranes were then rinsed in TBST three times and then treated with Western Lightning Plus ECL reagent (PerkinElmer, NEL105001EA) and exposed to GE Healthcare Hyperfilm ECL (28906839 ...
-
bioRxiv - Neuroscience 2022Quote: ... membranes were washed and incubated with the goat anti-rabbit IgG HRP-labeled secondary antibody (PerkinElmer, Waltham, MA, USA; NEF812001EA) for 1 h at room temperature ...
-
bioRxiv - Genetics 2022Quote: ... the membrane was transferred to a fresh falcon tube and incubated with 1ml of Western LightningPlus-ECL chemiluminescent reagent (PerkinElmer). Proteins bands were visualised with an iBright instrument (ThermoFisher) ...
-
bioRxiv - Cancer Biology 2022Quote: ... 15 or 10-20% SDS-PAGE gels and proteins were transferred onto PVDF membranes (0.45 μm, Perkin Elmer, Waltham, MA) and blocked with 5% BSA/TBS-T for 2 hours at room temperature ...
-
bioRxiv - Cancer Biology 2022Quote: ... the membranes were washed 5 min 3 times and developed using enhanced chemiluminiscence reagent (Western Lightning Plus-ECL; Perkin Elmer). The following primary antibodies were used ...
-
bioRxiv - Microbiology 2022Quote: ... The membranes were then incubated with anti-IgG rabbit antibodies conjugated to horseradish peroxidase (PerkinElmer Life Sciences (Boston, MA, USA), and developed using the Western Lightning Chemiluminescence Reagent Plus (PerkinElmer) ...
-
bioRxiv - Microbiology 2022Quote: Radioactivity of cell wall and membrane isolates was measured by scintillation counting using a Liquid Scintillation Analyzer Tri-Carb 4910 TR (PerkinElmer). To do this ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: Binding experiments were performed on membranes prepared from wild-type and HA-MOP-transfected HEK293 cells using [3H]DAMGO (51.7 Ci/mmol) (PerkinElmer NET902250UC) as described previously52 ...
-
bioRxiv - Molecular Biology 2024Quote: ... membranes were blocked with 5% BSA in Tris / HCl-buffered saline / 0.05% Tween (TBST) for 24 h at 4 °C and afterwards membranes were incubated for 1 h with HRP-Streptavidin (PerkinElmer; #NEL750001EA ...
-
bioRxiv - Neuroscience 2023Quote: ... the membrane was washed three times and Fixer-Developer (1:1) solution from Western Lightning Chemiluminescence Reagent Plus (PerkinElmer, NEL104001EA) enhanced chemiluminescence (ECL ...
-
bioRxiv - Developmental Biology 2023Quote: ... membrane staining was added to single wells immediately before imaging on an Opera Phenix™ High-Content Screening System (PerkinElmer).
-
bioRxiv - Neuroscience 2024Quote: ... The membranes were washed in TBST at least three times and probed with Western Lightning Plus ECL reagent (PerkinElmer, NEL105001EA) and exposed to GE Healthcare Hyperfilm ECL (28906839) ...
-
bioRxiv - Biochemistry 2024Quote: ... 10 µg RNA samples were separated on 2% glyoxal agarose gels and transferred onto nylon membranes (GeneScreen Plus, Perkin Elmer). Complementary [32P]-labeled probes were synthesized by linear PCR and hybridized at 65°C overnight in hybridization solution (0.5 M NaP pH = 7.0 ...
-
bioRxiv - Cell Biology 2024Quote: ... and membranes were incubated 1 min with in a 1:1 mixture of ECL solutions A and B (PerkinElmer, USA). Excess ECL solution was removed ...
-
bioRxiv - Neuroscience 2024Quote: ... the membranes were incubated for 90 seconds in 1 mL of enhanced chemiluminescence (ECL Pro) reagent (catalog number NEL122001EA, PerkinElmer Health Sciences Canada Inc. ...
-
bioRxiv - Biophysics 2021Quote: ... add 10 mL scintillation solution and measured the radioactivity retained on the membranes in liquid scintillation counter Tri-carb 4910TR (Perkin Elmer) [44].
-
bioRxiv - Cell Biology 2020Quote: ... the PVDF membrane was washed with TBST and incubated with appropriate secondary antibodies (horseradish peroxidase-conjugated goat anti-mouse or anti-rabbit IgG), followed by enhanced chemiluminescence (ECL; Visual protein, Taipei, Taiwan) and the membrane was examined by ECL spectrophotometry (Perkin Elmer, Geliance 600 Camera Imaging system ...
-
bioRxiv - Plant Biology 2020Quote: ... 10.5 μg of gDNA was digested with the restriction enzymes EcoRV and XbaI from New England Biolabs (http://www.neb.com) and blotted onto a nylon membrane (GeneScreenPlus; Perkin Elmer, http://www.perkinelmer.com) according to the manufacturer’s instructions.
-
bioRxiv - Pharmacology and Toxicology 2020Quote: Radioligand binding assays were performed with membranes prepared from cells expressing human CB2 or CB1 or mouse CB2 receptor using [3H]-CP55940 (Perkin Elmer) as a radioligand ...
-
bioRxiv - Biochemistry 2021Quote: ... The proteins were separated on 7% tris-glycine or 8% tricine gels by SDS-PAGE and transferred to a PVDF membrane (Perkin Elmer). The proteins were revealed using a V5-monoclonal antibody (V5-mAb V2660 ...
-
bioRxiv - Molecular Biology 2021Quote: ... total RNA was separated in 2% glyoxal agarose gels and blotted onto a positively charged nylon membrane (GeneScreen Plus, Perkin Elmer). [32P]-labelled probes specific for each transcript were generated by linear PCR ...
-
bioRxiv - Microbiology 2021Quote: ... After these incubations the membranes were washed again with PBS-T and proteins were visualized by Western Lightning Chemiluminescence Reagent Plus (Perkin Elmer).
-
bioRxiv - Microbiology 2022Quote: ... Samples were separated by SDS-PAGE using 10% or 15% (w/v) polyacrylamide gels and transferred to Protran nitrocellulose membranes (Perkin Elmer) using the Mini-Trans blot systems (BioRad ...
-
bioRxiv - Microbiology 2024Quote: ... Cultures were shaken for 24 hours at 250 RPM and at 37°C whereupon the Breathe Easy sealing membranes were removed and cell density was measured using OD600 in a Victor NIVO microplate reader (Perkin Elmer). OD600 of cell-free medium was removed from all cell density values ...
-
The conserved membrane-proximal domain of Sbh1/ Sec61β guides signal peptides into the Sec61 channelbioRxiv - Biochemistry 2024Quote: ... Proteins were labelled for 5 mins (for soluble proteins) or 15 mins (for membrane proteins) with 30 µCi of Express Protein Labelling Mix (Perkin Elmer) per sample at their restrictive temperature ...
-
bioRxiv - Molecular Biology 2022Quote: ... Membranes were finally washed three times with PBST for 5 min and protein levels were visualized by enhanced chemiluminescence (Perkin Elmer, NEL104001EA) according to the manufacturer’s instructions ...
-
bioRxiv - Biophysics 2020Quote: ... HEK cell membrane proteins (100 μg/ml final concentration) were incubated with 3 nM [3H]muscimol (30 Ci/mmol; PerkinElmer Life Sciences), neurosteroid (3 nM–30 μM ...
-
bioRxiv - Molecular Biology 2021Quote: ... HRP-conjugated secondary antibodies were diluted 1:5000 in TBS-T with 5% milk and incubated with the membrane at room temperature for an hour before performing the chemiluminescence reaction (Western Lightning Plus-ECL, Enhanced Chemiluminescence Substrate, Perkin Elmer NEL105001EA). Signal was captured with either X-ray films (CL-XPosure™ Film ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... The membrane mixture was then filtered over a 0.3% PEI pre-treated GF-B/C plate (#6005174, Perkin Elmer, Waltham, MA, USA) with a cell harvester system ...
-
bioRxiv - Bioengineering 2024Quote: ... and PCL-PNIPAAm-Cur membranes were characterised using a attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectrophotometer (Spectrum One, PerkinElmer, Llanstrisant, UK). The software Time Base (Spectrum ...