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Citations for PerkinElmer :
201 - 250 of 339 citations for SARS CoV 2 Nucleoprotein His tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... 293FT cells (2 × 104 cells) were plated in 96-well View Plates (Perkin Elmer Life Sciences, Waltham, MA, USA). Five hours later ...
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Secretin receptor as a target in gastrointestinal cancer: expression analysis and ligand developmentbioRxiv - Cancer Biology 2022Quote: ... to a concentration of 200 μM with an addition of 0.5 mCi carrier-free 125I-NaI in 2 μl of carrier solution (PerkinElmer). Iodination was initiated by addition of 4 μl chloramin-T solution (SigmaAldrich ...
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bioRxiv - Immunology 2021Quote: ... The SARS-CoV-2 spike protein antigen (2.0µg/mL) was directly adsorbed on half-area 96-well OptiPlate (96W HB, Perkin Elmer) microplates for 2h at 37°C in a humidified incubator.
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bioRxiv - Cell Biology 2020Quote: ... placed in polistor tubes with 2 ml of Ultima gold™XR liquid scintillation cocktail (Perkin Elmer, Massachusetts, USA) and counted in a scintillation counter ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were split on day 2 and 10,000 cells were seeded in each well (96 well-plate; CellCarrier-96 Ultra Microplates, Perkinelmer). At 12-14 h after cell seeding ...
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bioRxiv - Biochemistry 2021Quote: ... bound to albumin (1:1 molar ratio) or 0.1 μM [1-14C]oleic acid (2 μCi/dish Perkin Elmer) bound to albumin (1:1 molar ratio ...
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bioRxiv - Biochemistry 2021Quote: ... 20 μL of protein mixture was added to each well of a 1/2 area 96-well OptiPlate (PerkinElmer). Compounds were then added from DMSO stock to protein mixture (0.4 μl/well ...
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bioRxiv - Biochemistry 2021Quote: ... 20 μL of protein mixture was added to each well of a 1/2 area 96-well OptiPlate (PerkinElmer). FKBP12F36V competitor or p300-BRD competitor was then added from DMSO stock to protein mixture (0.4 μl/well ...
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bioRxiv - Biochemistry 2021Quote: ... pH 7.4) supplemented with 10% dialyzed FBS in the presence of 10 μCi of myo-[2-3H]inositol (PerkinElmer) for 10 hours ...
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bioRxiv - Microbiology 2022Quote: ... 10 μL of lipid extracts were mixed with 2 mL of Ultima Gold™ scintillation fluid (Perkin Elmer, USA), and radioactive counts were measured using a MicroBeta2® scintillation counter (Perkin Elmer ...
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bioRxiv - Microbiology 2023Quote: ... assays were carried out by incubating 500ng of methyltransferases Rv2067c or DOT1L with 1–2 μg of recombinant mammalian histones or MtHU and 80μCi 3H-SAM (PerkinElmer) in a buffer containing 50 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Biochemistry 2023Quote: ... 2-[1,2-3H(N)]-deoxy-D-glucose and [1,2-14C]-acetic acid were purchased from PerkinElmer (Waltham, MA, USA). PPARγ ...
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bioRxiv - Neuroscience 2023Quote: Mice were imaged for 2 minutes using a cooled charged-coupled device camera (IVIS Lumina; Perkin Elmer, Coventry, UK). Dams were anaesthetised with isoflurane as above.
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bioRxiv - Developmental Biology 2023Quote: ... ORO was measured by extracting lipids with a petrol ether/isopropanol mixture (3:2) and quantified for their absorbance at 490 nm for 0.1 s with Victor Spectrophotometer (PerkinElmer). Standard curve was applied and quantification was expressed in µg of pure ORO powder.
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bioRxiv - Bioengineering 2023Quote: ... FTIR of CNPs was measured in ATR (attenuated total reflectance) mode using a Spectrum 2 FTIR spectrometer from PerkinElmer. The scanning range for the measurements was 1000 cm-1 to 4000 cm-1.
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bioRxiv - Physiology 2023Quote: ... Measurement of radiolabelled 2-deoxyglucose was carried out in a Tri-Carb 4910 TR liquid scintillation analyzer (Perkin Elmer) by adding 200 μl of muscle lysate to 4 ml scintillation fluid (Ultima Gold ...
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bioRxiv - Immunology 2023Quote: ... Samples were incubated in triplicate (106 per sample) with 1 µCi of 2-[1,2-3H]-deoxyglucose (20 Ci/mmol; PerkinElmer) in uptake buffer for 4 min at room temperature and immediately spun through a layer of bromo-dodecane into a layer of 20% perchloric acid/8% sucrose to stop the reaction and separate cells from unincorporated 2-[3H]-deoxyglucose ...
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bioRxiv - Immunology 2024Quote: ... Tumor progression was subsequently monitored every 2-3 days using caliper measurement and the IVIS Spectrum imaging system (PerkinElmer) to measure bioluminescent signal after intraperitoneal administration of 0.15 mg of luciferin substrate per gram of body weight (PerkinElmer 122799) ...
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bioRxiv - Cell Biology 2024Quote: ... cells were incubated in 24-well plates for 2 h with 0.25 ml/well of fresh growth medium containing 5 μCi/ml [3H]adenine (PerkinElmer). The medium was then replaced with 0.5 ml/well of DMEM containing 20 mM HEPES ...
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bioRxiv - Cancer Biology 2021Quote: ... 50% and 25% MIA PaCa-2 cells) were grown in CellCarrier Spheroid ULA 96-well Microplates (6055330, PerkinElmer, Waltham, MA), as described previously ...
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bioRxiv - Cancer Biology 2021Quote: ... the Mia PaCa-2 cells and PCAFs were seeded in CellCarrier Spheroid ULA 96-well Microplates (6055330, PerkinElmer, Waltham, MA) with 0% ...
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bioRxiv - Molecular Biology 2020Quote: ... the reactions were started by adding 5× NTP mixture (1 mM for ATP, CTP and GTP, 0.1 mM for UTP) containing 2 µCi of [α−32P] UTP (PerkinElmer). Transcription reactions were performed at 37°C for 20 min ...
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bioRxiv - Plant Biology 2021Quote: ... leaf discs excised from the transformed leaves were incubated with 200 µM D-luciferin (Wako) for 2 h in white 24-well plates (PerkinElmer). Then ...
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bioRxiv - Microbiology 2021Quote: ... Plates were incubated in the dark for 2 h at RT before measuring fluorescence using the EnVision Multimode Plate Reader (PerkinElmer) with excitation and emission wavelengths of FITC 485 / FITC 535 ...
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bioRxiv - Genomics 2022Quote: ... The DNA extraction was performed at Hospital Universitari Vall d’Hebron (Supplementary Table 2) from blood samples using chemagic DNA Blood400 Kit LH (Perkin Elmer). The DNA from oral mucosa swabs ...
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bioRxiv - Microbiology 2019Quote: ... was achieved by addition of GDP- or UDP-GloTM nucleotide detection reagent following manufacturers’ instructions and analyzed via luminescence using a Victor 2 microplate reader (PerkinElmer).
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bioRxiv - Biophysics 2019Quote: ... After sharing at 600 rpm for 2 h at room temperature the plate transferred to a MicroBeta2 scintillation counter (PerkinElmer) left for 4 h to allow the SPA-beads to settle before quantifying 125I radioactivity ...
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bioRxiv - Physiology 2020Quote: On the fifth day of EOS or PBS treatment 3H-2-deoxy-D-glucose (2DG; 10uCi/mouse; PerkinElmer, Waltham, MA) was given to 4 hour fasted individually housed mice ...
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bioRxiv - Biochemistry 2021Quote: ... The filter paper was air dried for 1 hour and then placed into 2 ml of scintillation liquid (Ultima Gold, PerkinElmer), and radioactivity was counted using a scintillation counter (Tri-Carb B2810TR ...
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bioRxiv - Biochemistry 2021Quote: ... 50 nM of hNatD was mixed with 2 μM of radiolabeled [14C]acetyl-CoA (4 mCi/mmol; PerkinElmer Life Sciences), 20 μM of the peptide ...
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bioRxiv - Biochemistry 2020Quote: ... was counted using a PerkinElmer Tri-Carb 2800RT liquid scintillation counter after dissolving the filter in 2 ml of scintillation liquid (Emulsifier Scintillator Plus, PerkinElmer).
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bioRxiv - Cell Biology 2021Quote: ... buffer and 1.2 M sorbitol citrate solution, and then digested by 1.4 mg/ml 20T zymolyase (amsbio, 120491) and glusulase (PerkinElmer, NEE154001EA). Cells were spread on poly-L-lysine (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... 10mM MgCl2 and 1 mM MnCl2) supplemented with 10 μM ATP and 2 μCi γ-32P-ATP (blu002z250uc; Perkin Elmer) in 15μl reactions ...
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bioRxiv - Cancer Biology 2021Quote: ... we used initially the RNAscope Multiplex Fluorescent Assay version 1 with the fluorophores Alexa 488 and Atto 550 and in later experiments the RNAscope Multiplex Fluorescent Assay version 2 with the fluorophores Opal 570 and Opal 650 (PerkinElmer) according the manufacturer’s protocol for multiplex fluorescent in situ hybridization kit ...
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Cancer causes dysfunctional insulin signaling and glucose transport in a muscle-type specific mannerbioRxiv - Physiology 2021Quote: ... A fraction (50 μl) of the lysate was dissolved in 2 ml of β-scintillation liquid (Ultima Gold, Perkin Elmer) for measurement of 2-DG transport using [14C]mannitol to estimate extracellular space using β-scintillation counting ...
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bioRxiv - Cell Biology 2021Quote: ... The AlphaLISA™ assay was in 20 μL reactions (requiring only 2 μL cell culture media sample) in 384-well plate (6007290, PerkinElmer) following standard two-step no-wash protocol without any modifications ...
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AMPK is elevated in human cachectic muscle and prevents cancer-induced metabolic dysfunction in micebioRxiv - Molecular Biology 2022Quote: ... 2-deoxyglucose (2DG) uptake in tissues in vivo were measured by the retro-orbital injection of [3H]2DG (Perkin Elmer) diluted in saline ...
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bioRxiv - Cancer Biology 2022Quote: ... Images were acquired with an AxioObserver 2 mot plus fluorescence microscope (Zeiss, Munich, Germany) or with an Operetta high- content imaging system (PerkinElmer) at 5x magnification ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were then washed with Krebs-Ringer bicarbonate HEPES (KRBH) buffer and then given 400 μL with KRBH buffer loaded with 0.8 μCi/well [3H]-2-deoxyglucose (PerkinElmer, NET549A001MC) and 0.5 mM unlabeled 2-deoxyglucose and incubated at 37°C for 30 minutes ...
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bioRxiv - Microbiology 2020Quote: ... MOLT-4/CCR5 cells were added on day 2 and culture supernatants were examined for the p24 viral capsid protein by ELISA (PerkinElmer) after 14 ...
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bioRxiv - Cell Biology 2019Quote: GBM2 cells were plated at 4000 cells/well and exposed to epigenetic compounds (Table 2) at 10 µM for 1 day in 384-well optical bottom assay plates (PerkinElmer). Negative control was DMSO (0.1%) ...
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bioRxiv - Genetics 2019Quote: ... PRMT7 wild-type and mutants were incubated with putative substrates in PBS with 2 μCi of S-adenosyl-[methyl-3H]methionine ([3H]AdoMet) (55-85 Ci/mmol, PerkinElmer) for 18 h at 26°C ...
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bioRxiv - Microbiology 2019Quote: ... and 500 µl were transferred to 2 ml Eppendorf tubes and spiked with 2.5 µCi of 32P-orthophosphoric acid (Perkin Elmer). The cultures were grown for two generations (OD600 0.2 ...
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bioRxiv - Molecular Biology 2019Quote: ... was next added to the cultures and cells were labeled for 2 min with [2,8-3H]-adenine (NET06300 Perkin Elmer) at 0 ...
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bioRxiv - Bioengineering 2020Quote: Bioprinted SK-N-BE(2) spheroids were prepared as described earlier in a 96-well plate (Cell-Carrier, Perkin Elmer). After incubating the plate for 3 days ...
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bioRxiv - Molecular Biology 2021Quote: ... Plates were incubated in the dark for 2 h at room temperature before measuring fluorescence using the EnVision Multimode Plate Reader (PerkinElmer), as described previously74 ...
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bioRxiv - Molecular Biology 2021Quote: ... 6 pmole trypSL (1.25 µl from a 50µM stock was kinased with 2 µl 32PgammaATP (25 µM, 6000Ci/mmol, 150 mCi/ml, Perkin Elmer) with 10 U PNK in 10 µl with 20 U RNasin (Roche) ...
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bioRxiv - Microbiology 2021Quote: ... TZM-bl reporter cells were seeded at a density of 2 × 104 cells/well in 96-well luminometer-compatible tissue culture plates (PerkinElmer) 24 h before infection ...
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bioRxiv - Biochemistry 2022Quote: ... 5 μL time points were taken and quenched with 1.33 mM EDTA then brought to 20 μL final volume for detection using 1 nM LANCE Ultra Europium anti-H3K4me1/2 antibody (TRF0402, PerkinElmer) and 50 nM LANCE Ultra Ulight-Streptavidin (TRF0102 ...
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bioRxiv - Immunology 2022Quote: ... Virus–monoclonal antibody-containing medium was then aspirated from cells and 100Lµl of a 1:2 dilution of SteadyLite Plus (PerkinElmer, 6066759) in PBS with Ca2+ and Mg2+ was added to cells ...