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Citations for PerkinElmer :
201 - 250 of 260 citations for Phosphatidylinositol N acetylglucosaminyltransferase subunit Q PIGQ Antibody HRP since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2024Quote: ... Horseradish peroxidase or biotin-conjugated secondary antibodies and a Tyramide signal amplification kit (PerkinElmer) were used after incubating the primary antibodies ...
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bioRxiv - Immunology 2024Quote: ... 1:20000 Eu3+ cryptate conjugated to a His6-specific antibody (HTRF donor, PerkinElmer AD0402) and 1:500 Streptavidin-XL665 (HTRF acceptor ...
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bioRxiv - Cancer Biology 2021Quote: ... Endogenous peroxidase was then blocked by incubation in Antibody Diluent/Block (PerkinElmer #72424205, Massachusetts, USA) for 10 minutes ...
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bioRxiv - Microbiology 2020Quote: ... and the bound antibodies were detected using enhanced chemiluminescence solution (Perkin Elmer, Waltham, MA, USA). Images were acquired using the ChemoCam 6.0 ECL system (INTAS Science Imaging ...
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bioRxiv - Microbiology 2022Quote: ... Horseradish peroxidase-conjugated goat anti-rabbit polyclonal and anti-mouse antibodies were purchased from PerkinElmer Life Sciences (Boston ...
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bioRxiv - Cancer Biology 2020Quote: ... and bound primary antibodies were visualized using Western Lightning Plus-ECL (PerkinElmer Inc., Waltham, MA). The primary antibodies used in this study were as follows ...
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bioRxiv - Developmental Biology 2021Quote: ... RRID: AB_2340590) were used as secondary antibody and blots were detected using ECL detection (PerkinElmer (PE), NEL105001EA) ...
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bioRxiv - Neuroscience 2020Quote: ... POD-conjugated antibody labeling of mRNA was proceeded with TSA Plus DNP signal amplification (Perkin Elmer) and a subsequently labeling with anti-DNP-KLH-488 antibodies (1:1000 ...
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bioRxiv - Developmental Biology 2022Quote: ... Then those sections were stained with anti-MYC antibody with TSA plus fluorescein system (Perkin Elmer) followed by antigen retrieval to remove antibodies ...
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bioRxiv - Microbiology 2021Quote: ... the membrane was probed with the appropriate antibodies and detected with Western Lightning Plus-ECL (PerkinElmer) using a Gbox (SYNGENE) ...
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bioRxiv - Cancer Biology 2022Quote: ... Blots were probed overnight at 4 °C with indicated antibodies and developed by chemiluminescence (Perkin Elmer).
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bioRxiv - Physiology 2024Quote: ... Fluorescein was detected with a horseradish peroxidase-conjugated anti-fluorescein antibody (RRID: AB_2737388; PerkinElmer, Waltham, MA) and visualized by using the TSA Plus Fluorescein System (PerkinElmer) ...
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bioRxiv - Developmental Biology 2023Quote: ... The Lmx1a antibody detection capability was improved using the Tyramide Signal Amplification kit (TSA, Perkin Elmer), in accordance with manufacturers instructions.
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bioRxiv - Developmental Biology 2020Quote: ... Signals from anti-OAZ and Lmx1a antibodies were amplified using the Tyramide Signal Amplification Kit (Perkin Elmer), according to manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... The mean positive area was determined using appropriate isotype control antibodies and quantified using Volocity (Perkin Elmer) and Fiji (NIH ...
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bioRxiv - Neuroscience 2020Quote: ... For CC3 staining secondary antibody was followed by signal amplification using TSA Plus Cyanine 3 System (PerkinElmer). Following the incubation with the secondary antibody ...
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bioRxiv - Cancer Biology 2021Quote: ... Antigen-antibody complexes were detected with West Pico Chemi luminescent substrate using standard manufactures protocol (Perkin Elmer). Imaging of all western blots was performed using an UVP BioImager 600 system equipped with VisionWorks Life Science software (UVP ...
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bioRxiv - Cancer Biology 2021Quote: ... followed by primary antibody and corresponding secondary horseradish peroxidase-conjugated polymer (Zytomed Systems, Germany or Perkin Elmer). Each horseradish peroxidase-conjugated polymer mediated the covalent binding of different fluorophore using tyramide signal amplification ...
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bioRxiv - Physiology 2024Quote: ... fluorescein was detected with an anti-fluorescein antibody conjugated to horseradish peroxidase (RRID: AB_2737388; PerkinElmer, Waltham, MA) and visualized using the TSA Plus Fluorescein System (PerkinElmer) ...
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bioRxiv - Neuroscience 2023Quote: ... the binding sites of the secondary antibodies were detected using Western Lightning ECL Pro (PerkinElmer, MA, USA). Chemiluminescence was visualized using the Fusion Solo S imaging system (Vilber Bio Imaging ...
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bioRxiv - Neuroscience 2023Quote: ... Fluorescein was detected with an anti-fluorescein antibody conjugated to horseradish peroxidase (RRID: AB_2737388; PerkinElmer, Waltham, MA) and visualized using the TSA Plus Fluorescein System (PerkinElmer) ...
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bioRxiv - Biochemistry 2024Quote: ... The pellets were washed with PBS pH 7.4 + 0.1% BSA and then incubated with anti-FLAG antibody conjugated to allophycocyanin (anti-FLAG:APC; PerkinElmer) (diluted 1:100 into PBS pH 7.4 + 0.1% BSA ...
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bioRxiv - Immunology 2020Quote: ... The integrated signal density corresponding to labeled antigen or antibody in each LN was calculated using LivingImage (PerkinElmer) or ImageJ software and plotted using GraphPad Prism 8 ...
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bioRxiv - Immunology 2023Quote: ... After secondary antibody incubation cells were directly imaged and analyzed using Operetta CLS High Content Analysis System (PerkinElmer).
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bioRxiv - Neuroscience 2021Quote: ... Slides were washed again and incubated with the appropriate Opal tertiary antibody (1:100 in amplification diluent, Perkin Elmer) for 60 min ...
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bioRxiv - Developmental Biology 2020Quote: ... incubated 1h in blocking buffer and then incubated overnight with an anti-DNP-POD antibody (1:1000, Perkin Elmer). The next day embryos were washed in PBS-Tween ...
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bioRxiv - Cell Biology 2023Quote: ... 10 nM full-length 6x-His-tagged human G3BP1 produced in mammalian cells was added to the reaction mix containing test compound and 0.5 nM anti-His-Tb antibody (PerkinElmer). Assays were initiated by adding 4 nM FAM-labeled PEG6-USP10 24-mer peptide probe (FAM-PEG6-GALHSPQYIFGDFSPDEFNQFFVT ...
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bioRxiv - Developmental Biology 2023Quote: ... was used to detect the anti-pH3 antibody after detection of ISH probe by using TSA plus (Perkin Elmer) green (FP1168).
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bioRxiv - Immunology 2024Quote: ... or HIS-SUMO-C-A mutant Rb 380-785 was pre-incubated with 0.4 nM HTRF donor conjugated antibodies anti-GST-Tb or anti-HIS-Tb (PerkinElmer), respectively ...
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bioRxiv - Immunology 2021Quote: ... Immunoreactive proteins were detected with either anti-mouse or anti-rabbit horseradish peroxidase-coupled secondary antibody followed by detection with enhanced chemiluminescence (PerkinElmer).
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bioRxiv - Neuroscience 2021Quote: ... Molecular weight bands were visualized by incubation with peroxidase-conjugated anti-mouse or anti-rabbit secondary antibody (1:5000; Jackson Immuno Research Laboratories) followed by treatment with the ECL-plus reagent (Western Lighting; PerkinElmer) and exposure on G:Box Chemi XRQ system (Syngene) ...
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bioRxiv - Neuroscience 2021Quote: ... and Lumi4-Tb cryptate conjugated anti-IP1 antibody (emission at 620 nm) was detected using an Envision plate reader (PerkinElmer). Results were calculated from the 665/620 nm ratio and normalised to the maximum response stimulated by acetylcholine.
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bioRxiv - Neuroscience 2022Quote: ... membranes and immunoblots were carried out using primary antibodies (Table S4) followed by incubation with appropriate secondary horseradish peroxidase-conjugated antibodies (Table S5) and chemoluminiscent detection (Western Lightning, PerkinElmer). All antibodies were diluted in PBS containing 5% semi-skimmed milk and 0.1% Tween-20 ...
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bioRxiv - Neuroscience 2020Quote: ... anti-mouse or anti-rabbit secondary antibody (1:10000; Jackson Immuno Research Laboratories) followed by treatment with ECL-plus reagent (Western Lighting, PerkinElmer) and exposure to autoradiography films ...
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bioRxiv - Microbiology 2022Quote: ... The membranes were then incubated with anti-IgG rabbit antibodies conjugated to horseradish peroxidase (PerkinElmer Life Sciences (Boston, MA, USA), and developed using the Western Lightning Chemiluminescence Reagent Plus (PerkinElmer) ...
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bioRxiv - Neuroscience 2022Quote: ... then incubated for 30 min at 25 °C with shaking at 400 RPM with 9 ng/well of DELFIA Eu-N1 anti-mouse antibody in DELFIA assay buffer (PerkinElmer), washed with 3 × 100 μl of PBST before enhancing with 80 μl of DELFIA Enhancement Solution (PerkinElmer) ...
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bioRxiv - Biochemistry 2022Quote: ... 5 μL time points were taken and quenched with 1.33 mM EDTA then brought to 20 μL final volume for detection using 1 nM LANCE Ultra Europium anti-H3K4me1/2 antibody (TRF0402, PerkinElmer) and 50 nM LANCE Ultra Ulight-Streptavidin (TRF0102 ...
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bioRxiv - Immunology 2022Quote: ... Virus–monoclonal antibody-containing medium was then aspirated from cells and 100Lµl of a 1:2 dilution of SteadyLite Plus (PerkinElmer, 6066759) in PBS with Ca2+ and Mg2+ was added to cells ...
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bioRxiv - Microbiology 2024Quote: ... Blots were probed with the indicated antibodies and developed using the Western Lighting plus ECL (Perkin Elmer, Waltham, MA, USA). Blots were imaged on the Bio-Rad ChemiDoc MP Imaging System (BioRad ...
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bioRxiv - Biochemistry 2023Quote: ... FLAG and HA were detected using the LI-COR system and HRP-conjugated GAPDH primary antibody was incubated with blots for 2 hours at room temperature and detected with Western Lightning Plus-ECL (PerkinElmer) using a Bio-Rad Chemidoc System.
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bioRxiv - Cell Biology 2020Quote: ... involving the use of a biotinylated secondary antibody followed by an ABC complex and Cy3-tyramide kit (Perkin Elmer, cat#: FP1046) or an Alexa secondary antibody (1:500 ...
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bioRxiv - Bioengineering 2020Quote: ... Images are acquired by confocal microscopy to minimize auto-fluorescence of unlabeled dye/antibody, using a conventional laser scanning confocal microscope (CLSM510Meta, Zeiss) or a high-content screening system (Opera Phenix, Perkin Elmer). Instrument specifications are found in Supplemental Information ...
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bioRxiv - Microbiology 2023Quote: ... The membrane was probed with a goat anti-mouse antibody conjugated to horseradish peroxidase and bands were visualized using the Western Lighting Plus-ECL reagent (Perkin Elmer). When performing the KatG expression analysis in response to C10 treatment ...
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bioRxiv - Microbiology 2022Quote: ... and serum IgA antibodies to the secreted form of S-2P were measured by europium ion-enhanced DELFIA-TRF assay (Perkin Elmer) following the supplier’s protocol.
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bioRxiv - Cell Biology 2023Quote: ... or ECL anti-rabbit antibodies (NA934V; 17434242) (Amersham) were used for detection of probed proteins using enhanced chemiluminescence (ECL)(Perkin Elmer). Chemiluminescence was detected using an iBright FL1500 (ThermoFisherScientific ...
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bioRxiv - Neuroscience 2021Quote: ... The bound primary antibodies were detected by horseradish peroxidase-conjugated secondary antibodies in combination with an enhanced chemiluminescence detection system using Western Lightning Chemiluminescence Reagent (PerkinElmer Life Sciences) or ImmunoStar (Wako Pure Chemical Industries) ...
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bioRxiv - Neuroscience 2020Quote: ... Double immunofluorescence was performed using tetramethyl rhodamine labelled secondary antibody for alpha-synuclein and NSF with the fluorescein signal amplification kit (Perkin Elmer, UK). Following adequate washes to remove background fluorescence ...
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bioRxiv - Neuroscience 2021Quote: ... the sections were incubated with a horseradish peroxidase-conjugated anti-fluorescein antibody (diluted 1:500 with DIG-1, PerkinElmer, Foster City, CA) for 1 h and washed twice with DIG-1 for 5 min each time ...
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bioRxiv - Biochemistry 2022Quote: ... Detection reagents were added stepwise with 30 min incubation of anti-H3K4me3 antibody and Ulight-Streptavidin with reaction time points followed by 1 hr incubation with Europium anti-rabbit antibody in 384 well white microplates (PerkinElmer OptiPlate-384). TR-FRET emission at 665 nm and at 615 nm by 320 nm excitation with 50 μs delay and 100 μs integration time was measured using a Molecular Devices SpectraMax M5e plate reader ...
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bioRxiv - Immunology 2024Quote: ... Five-micrometer serial sections were stained with a human-specific anti-COXIV antibody and scanned using the Vectra® Polaris Automated Imaging System (PerkinElmer, USA) at 20×magnification ...