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Citations for PerkinElmer :
201 - 250 of 467 citations for Borrelia burgdorferi C p Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Adaptive translational pausing is a hallmark of the cellular response to severe environmental stressbioRxiv - Molecular Biology 2020Quote: ... followed by incubation with 35S-Met/Cys (30 mCi/mL EXPRESS Protein Labeling Mix, PerkinElmer) for an additional 30 min ...
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bioRxiv - Cell Biology 2019Quote: ... Viral titer was determined using p24 ELISA test measuring viral capsid protein p24 (Perkin Elmer, performed in Biomedicum Functional Genomics Unit) ...
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bioRxiv - Cell Biology 2020Quote: ... protein signals were detected via enhanced chemiluminescence (Western Lightning Plus ECL, Perkin Elmer, Waltham, MA) followed by exposure to film (CL-XPosure ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were detected with the chemiluminescence detection reagent Perkin Western Lightning plus ECL (Perkin Elmer) and visualized using a radiology film processor (Curix 60 ...
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bioRxiv - Microbiology 2022Quote: ... in the presence of EasyTag EXPRESS 35S Protein Labelling Mix containing [35S] methionine (Perkin Elmer) (0.533 MBq ...
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bioRxiv - Molecular Biology 2023Quote: ... in total volume of 18 μl using Protein A acceptor and Streptavidin donor beads (PerkinElmer). Varying amounts of the prefusion and postfusion form of RSV F protein in PBS supplemented with mucin (10 mg/L) ...
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bioRxiv - Cell Biology 2023Quote: ... Protein detection from the membranes was performed with Western Lightning ECL Pro substrate (PerkinElmer NEL121001EA).
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bioRxiv - Cell Biology 2023Quote: ... Protein expression was detected using the Western Lighting Plus-ECL substrate from PerkinElmer (Waltham, MA) on a G:BOX Chemi XX6 gel doc system (Syngene) ...
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bioRxiv - Immunology 2024Quote: ... The target proteins were visualized by Western Lightning PLUS-ECL or Ultra kit (Perkin Elmer). The images were acquired by Azure Biosystems C300 or Thermo Fisher Scientific iBright FL1000 ...
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bioRxiv - Genetics 2019Quote: ... and then subjected to spectrophotometric analysis for isolated respiratory chain complex activities at 37°C using a spectrophotometer (PerkinElmer). Complex II activity was measured at 600 nm (ε = 19.1 mmol-1cm-1 ...
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bioRxiv - Biophysics 2020Quote: ... depending on the mutant) were incubated for 1.5 hr at 30°C with [3H]-spiperone (Perkin Elmer, Waltham, MA) (0.02–12 nM ...
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bioRxiv - Microbiology 2022Quote: ... one ml of culture was labelled for 4 min at 30 °C using 30 μCi γ-[32P]ATP (PerkinElmer). Samples were incubated with 6% sucrose ...
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bioRxiv - Developmental Biology 2022Quote: ... Relative luminescence units (RLU) were detected on a pre-warmed 37°C EnVision plate reader (Perkin Elmer, Waltham, MA) for 30 min to reach the maximum value ...
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bioRxiv - Immunology 2019Quote: ... Measurements of intracellular calcium were carried out at 37°C in a PerkinElmer fluorescence spectrophotometer (LC50, Perkin Elmer, USA), with excitation wavelengths of 340 and 380 nm ...
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bioRxiv - Microbiology 2021Quote: ... After 48 hours of incubation at 37°C, luciferase activity was measured (Fluoroskan Accent, Labsystems) using Brite-Lite (PerkinElmer). Uninfected TZM-bl cells were used as a negative control ...
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bioRxiv - Molecular Biology 2022Quote: ... A fragmentation step was carried out for 6.5 minutes at 94°C and 7 μM NextFlex DNA barcodes (PerkinElmer) were used for adapter ligation ...
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bioRxiv - Neuroscience 2022Quote: ... the incubations were terminated by rapid filtration through 0.5% (v/v) polyethylenimine pre-soaked GF/C filterplates using a 96-well Filtermate harvester (PerkinElmer), followed by four rapid washes with ice-cold binding buffer ...
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bioRxiv - Cell Biology 2023Quote: ... for 1 h at 37 °C and imaged with the Opera Phenix High Content Screening System (#HH14000000, Perkin Elmer). Number of counted nuclei was determined with the image analysis software Harmony (PerkinElmer ...
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bioRxiv - Developmental Biology 2023Quote: ... Relative luminescence units (RLU) were detected on a pre-warmed 37°C EnVision plate reader (Perkin Elmer, Waltham, MA) for 30 min to reach the maximum value ...
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bioRxiv - Neuroscience 2023Quote: ... for 30 min at 37°C with 1000 rpm shaking using a DELFIA plate shaker (Perkin Elmer, Waltham, MA) and placed in a 37°C incubator ...
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bioRxiv - Cell Biology 2020Quote: ... and medium was exchanged for Leibovitz L-15 CO2-independent medium for imaging at 37°C on a spinning disc confocal system (Ultraview Vox, PerkinElmer) with a 100 × 1.4 NA oil-immersion objective ...
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bioRxiv - Microbiology 2020Quote: ... the plates containing infected macrophages were incubated at 37°C with 5% CO2 for 18 h in an Operetta High-Content Imaging System (PerkinElmer). During this 18 h incubation ...
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bioRxiv - Microbiology 2019Quote: ... which were incubated with various concentration of synthetic biotinylated TAR-RNA (biotin 5’-dT/GGU CUC UCU GGU UAG ACC AGA UCU GAG CCU GGG AGC UCU CUG GCU AAC UAG GGA ACC/3’-biotin dT) (Integrated DNA Technologies) at 4°C for 3 h in the AlphaLISA buffer (PerkinElmer). Anti-His Donor and Streptavidin Acceptor AlphaLISA beads (PerkinElmer ...
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bioRxiv - Developmental Biology 2021Quote: ... the cells were incubated for 5 minutes at 37°C with 55µCi/well of 35S-L-methionine and 35S-L-cysteine Promix (Perkin Elmer) in a minimal volume of culture medium ...
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bioRxiv - Cell Biology 2022Quote: Images of embryos were acquired at 23°C using a PerkinElmer ERS spinning disk confocal system mounted on a Zeiss Axiovet 200M microscope using Volocity software (PerkinElmer). A 63X ...
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bioRxiv - Biophysics 2022Quote: ... incubated for 4 h at 37°C and fluorescent signal intensity was quantified using the EnSpire Multimode Plate Reader (PerkinElmer) at excitation and emission wavelengths of 540 and 612 nm respectively.
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bioRxiv - Cell Biology 2022Quote: ... cell motility was acquired using temperature (37°C) and CO2 (5%) controlled Operetta CLS High-Content Analysis System (PerkinElmer, HH16000000) (non-confocal ...
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bioRxiv - Immunology 2019Quote: ... 0.1% BSA) and subsequently incubated for 30 min at 25°C with Nickel Chelate acceptor beads (Perkin Elmer, Waltham, MA) and Streptavidin conjugated donor beads (Perkin Elmer) ...
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bioRxiv - Cell Biology 2019Quote: ... and media exchanged for Leibovitz L-15 CO2-independent medium for imaging at 37 °C on a spinning disc confocal system (Ultraview Vox; PerkinElmer) with a 100× 1.4 NA oil-immersion objective ...
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bioRxiv - Molecular Biology 2019Quote: ... Reactions containing 200 ng of mRNA and 0.1 mM of D-luciferin were carried out in 5 μL aliquots at 37°C for 15 minutes and the activity of in vitro synthesized luciferase was measured by VictorX5 (PerkinElmer) every 30 seconds.
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bioRxiv - Biochemistry 2021Quote: ... Fluorescence intensity (Ex = 485 nm/Em = 535 nm) was monitored at 37 °C with a microplate reader VictorIII (Perkin Elmer). A calibration curve was created by measuring multiple concentrations (from 0.001 to 5 μM ...
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bioRxiv - Biochemistry 2021Quote: ... The samples were boiled at 95°C for 10 min and subjected directly to LabChip GX II capillary gel electrophoresis (PerkinElmer) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... TR-FRET readout was performed after 1 h incubation at 4°C using an EnVision 2105 Multimode Plate Reader (PerkinElmer) with previously defined measurement parameters53 ...
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bioRxiv - Cancer Biology 2020Quote: ... Live-cell imaging was performed in a humidified environment at 37°C using a spinning-disc confocal microscopy system (UltraVIEW Vox; PerkinElmer) and a charge-coupled device camera (ORCA-R2 ...
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bioRxiv - Cancer Biology 2021Quote: Bioluminescence signals in exosomes and MVs (3 × 107 EVs/µL) carrying PalmReNL were measured at room temperature (25°C) using a VICTOR Nivo Microplate Reader (PerkinElmer). EVs were incubated in the presence or absence of 1% Triton X-100 for 30 min at 37°C ...
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bioRxiv - Molecular Biology 2020Quote: ... with the confocal setting at 40X magnification at 37°C and 5% CO2 and analyzed with Columbus Image Analysis System (PerkinElmer). Each well was excited with 425 nm light and emissions detected at 434-515 nm for CFP and 500-550 nm for YFP ...
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bioRxiv - Neuroscience 2022Quote: ... To quantify α1-3;5GABAAR79 sections were incubated for 60 minutes at 4°C in 1nM [3H]flumazenil (Perkin Elmer, NET757001MC), or in 1nM [3H]flumazenil with 10 µM flunitrazepam (Sigma Aldrich ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and 20 μl of the reaction mixture was incubated at 26°C for 1 h in a 384-well AlphaPlate (PerkinElmer). Subsequently ...
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bioRxiv - Biochemistry 2021Quote: ... microsomes prepared from HEK293 cells expressing RyR2 were incubated for 1 h at 25 °C with 5 nM [3H]ryanodine (PerkinElmer) in reaction media containing 0.17 M NaCl ...
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bioRxiv - Cell Biology 2020Quote: Embryos and third instar larval larval brains were imaged at 21°C on a Perkin Elmer ERS spinning disk (Volocity software version 6.3, PerkinElmer Inc.) mounted on a Zeiss Axiovert 200M microscope using a 63X/1.4-NA oil immersion objective and an Orca ER CCD camera (Hamamatsu Photonics) ...
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Identification and biochemical characterization of a novel eukaryotic-like Ser/Thr kinase in E. colibioRxiv - Microbiology 2020Quote: ... Reactions were incubated at 25 °C for 10mins followed by addition of 5 μCi of [γ-32P] ATP(Perkin Elmer). Reactions were incubated for additional 30 mins at 37 °C and were stopped using 3X Laemmli buffer ...
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bioRxiv - Microbiology 2022Quote: ... maintained at 200 °C and with N2 as the gas vector (flow rate of 6 mL·min-1) with a flame ionization detector (FID) maintained at 280 °C (PerkinElmer, USA)
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bioRxiv - Bioengineering 2022Quote: ... The volatiles were desorbed again by heating (200°C) in an Automatic Thermal Desorber (ATD-400, Perkin Elmer, Norwalk, CN), cryofocused on a cold trap (-30 °C) ...
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bioRxiv - Biochemistry 2022Quote: ... Steady-state binding signal was obtained after incubation of reactions for 1 hr at 25° C using a EnSight Multimode plate reader (PerkinElmer) with a 30 ms excitation at 680 nm and emission measured at 615 nm after 140 ms ...
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bioRxiv - Molecular Biology 2022Quote: ... RNA samples were separated on a 7 M urea denaturing polyacrylamide gel at 4°C at 100V and transferred onto a charged nylon transfer membrane (PerkinElmer), followed by cross-linking using a UV Stratalinker and drying at 80°C on a Gel Dryer (Bio-Rad).
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Plates were incubated in the dark for 1 hr at 37°C followed by measurement of fluorescence using an Envision plate reader (PerkinElmer) with standard AlphaScreenTM settings ...
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bioRxiv - Cell Biology 2022Quote: ... The plate was sealed with parafilm and placed in a pre-heated (37°C) Opera Phenix microscope with 40x or 60x water-immersion lens (PerkinElmer) with 5% CO2.
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bioRxiv - Molecular Biology 2022Quote: ... was next added and incubated with the mixture at 4 °C for 1 hour before the measurements performed on Envision Readers (PerkinElmer). The fluorescently-labeled m6A-containing mRNA was used to adjust the gain factor ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were washed with PBS and then stored in PBS at 4°C until imaged in the Opera Phenix (PerkinElmer) at 40x magnification and images analysed using a Harmony software to assess protein expression and morphology (see supplementary).
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bioRxiv - Genomics 2022Quote: ... nuclei were extracted from ~2×107 cells and run-on reactions were carried on at 37°C for 3 minutes using 3μl of each 11-biotin-labelled ribonucleotide stocks (Perkin Elmer). Following real time PCR amplification in the presence of EvaGreen dye as above ...