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Citations for PerkinElmer :
201 - 250 of 716 citations for 5 2 Chloronicotinoyl 2 furoic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... leaf discs excised from the transformed leaves were incubated with 200 µM D-luciferin (Wako) for 2 h in white 24-well plates (PerkinElmer). Then ...
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bioRxiv - Microbiology 2021Quote: ... Plates were incubated in the dark for 2 h at RT before measuring fluorescence using the EnVision Multimode Plate Reader (PerkinElmer) with excitation and emission wavelengths of FITC 485 / FITC 535 ...
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bioRxiv - Genomics 2022Quote: ... The DNA extraction was performed at Hospital Universitari Vall d’Hebron (Supplementary Table 2) from blood samples using chemagic DNA Blood400 Kit LH (Perkin Elmer). The DNA from oral mucosa swabs ...
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bioRxiv - Physiology 2020Quote: On the fifth day of EOS or PBS treatment 3H-2-deoxy-D-glucose (2DG; 10uCi/mouse; PerkinElmer, Waltham, MA) was given to 4 hour fasted individually housed mice ...
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bioRxiv - Biochemistry 2021Quote: ... The filter paper was air dried for 1 hour and then placed into 2 ml of scintillation liquid (Ultima Gold, PerkinElmer), and radioactivity was counted using a scintillation counter (Tri-Carb B2810TR ...
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bioRxiv - Biochemistry 2021Quote: ... 50 nM of hNatD was mixed with 2 μM of radiolabeled [14C]acetyl-CoA (4 mCi/mmol; PerkinElmer Life Sciences), 20 μM of the peptide ...
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bioRxiv - Biochemistry 2020Quote: ... was counted using a PerkinElmer Tri-Carb 2800RT liquid scintillation counter after dissolving the filter in 2 ml of scintillation liquid (Emulsifier Scintillator Plus, PerkinElmer).
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bioRxiv - Cell Biology 2021Quote: ... buffer and 1.2 M sorbitol citrate solution, and then digested by 1.4 mg/ml 20T zymolyase (amsbio, 120491) and glusulase (PerkinElmer, NEE154001EA). Cells were spread on poly-L-lysine (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... 10mM MgCl2 and 1 mM MnCl2) supplemented with 10 μM ATP and 2 μCi γ-32P-ATP (blu002z250uc; Perkin Elmer) in 15μl reactions ...
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bioRxiv - Cancer Biology 2021Quote: ... we used initially the RNAscope Multiplex Fluorescent Assay version 1 with the fluorophores Alexa 488 and Atto 550 and in later experiments the RNAscope Multiplex Fluorescent Assay version 2 with the fluorophores Opal 570 and Opal 650 (PerkinElmer) according the manufacturer’s protocol for multiplex fluorescent in situ hybridization kit ...
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Cancer causes dysfunctional insulin signaling and glucose transport in a muscle-type specific mannerbioRxiv - Physiology 2021Quote: ... A fraction (50 μl) of the lysate was dissolved in 2 ml of β-scintillation liquid (Ultima Gold, Perkin Elmer) for measurement of 2-DG transport using [14C]mannitol to estimate extracellular space using β-scintillation counting ...
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bioRxiv - Cell Biology 2021Quote: ... The AlphaLISA™ assay was in 20 μL reactions (requiring only 2 μL cell culture media sample) in 384-well plate (6007290, PerkinElmer) following standard two-step no-wash protocol without any modifications ...
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AMPK is elevated in human cachectic muscle and prevents cancer-induced metabolic dysfunction in micebioRxiv - Molecular Biology 2022Quote: ... 2-deoxyglucose (2DG) uptake in tissues in vivo were measured by the retro-orbital injection of [3H]2DG (Perkin Elmer) diluted in saline ...
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bioRxiv - Cancer Biology 2022Quote: ... Images were acquired with an AxioObserver 2 mot plus fluorescence microscope (Zeiss, Munich, Germany) or with an Operetta high- content imaging system (PerkinElmer) at 5x magnification ...
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bioRxiv - Microbiology 2020Quote: ... MOLT-4/CCR5 cells were added on day 2 and culture supernatants were examined for the p24 viral capsid protein by ELISA (PerkinElmer) after 14 ...
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bioRxiv - Bioengineering 2020Quote: Bioprinted SK-N-BE(2) spheroids were prepared as described earlier in a 96-well plate (Cell-Carrier, Perkin Elmer). After incubating the plate for 3 days ...
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bioRxiv - Molecular Biology 2021Quote: ... Plates were incubated in the dark for 2 h at room temperature before measuring fluorescence using the EnVision Multimode Plate Reader (PerkinElmer), as described previously74 ...
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bioRxiv - Molecular Biology 2021Quote: ... 6 pmole trypSL (1.25 µl from a 50µM stock was kinased with 2 µl 32PgammaATP (25 µM, 6000Ci/mmol, 150 mCi/ml, Perkin Elmer) with 10 U PNK in 10 µl with 20 U RNasin (Roche) ...
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bioRxiv - Microbiology 2021Quote: ... TZM-bl reporter cells were seeded at a density of 2 × 104 cells/well in 96-well luminometer-compatible tissue culture plates (PerkinElmer) 24 h before infection ...
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bioRxiv - Biochemistry 2022Quote: ... 5 μL time points were taken and quenched with 1.33 mM EDTA then brought to 20 μL final volume for detection using 1 nM LANCE Ultra Europium anti-H3K4me1/2 antibody (TRF0402, PerkinElmer) and 50 nM LANCE Ultra Ulight-Streptavidin (TRF0102 ...
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bioRxiv - Immunology 2022Quote: ... Virus–monoclonal antibody-containing medium was then aspirated from cells and 100Lµl of a 1:2 dilution of SteadyLite Plus (PerkinElmer, 6066759) in PBS with Ca2+ and Mg2+ was added to cells ...
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bioRxiv - Microbiology 2023Quote: ... A total well volume of approximately 112.5 μL was incubated at room temperature for 2 minutes prior to measuring luminescence and OD600 readings on an EnVision plate reader (PerkinElmer).
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bioRxiv - Immunology 2023Quote: ... The bioluminescent signal emitted by the disseminated tumors was measured at 7-day intervals for 2 months using the IVIS Spectrum In Vivo Imaging System (PerkinElmer). The weight of the mice was recorded before each measurement.
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bioRxiv - Cancer Biology 2023Quote: ... cells were imaged for 2 hr prior to and 12 hr following TNFα addition using the Opera Phenix Plus High-Content Screening System (PerkinElmer), with two biological replicates (n = 2) ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were imaged for 2 hr prior to and 48 hr following TNFα addition using the Opera QEHS imaging system (PerkinElmer). Nuclear RELA and PCNA intensities were measured using Nuclitrack software (Cooper et al. ...
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Cancer-associated fibroblasts produce matrix-bound vesicles that influence endothelial cell functionbioRxiv - Cancer Biology 2023Quote: ... 25-45 images were acquired on an Opera Phenix high-content imaging system (objective 20x, z-stacks of 2 μm, PerkinElmer). Image analysis was performed using Harmony imaging analysis software (PerkinElmer ...
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bioRxiv - Biochemistry 2023Quote: cKO cells grown in the presence or absence of acetamide normalized to OD600 = 1 were incubated for 2 hours at 37 °C in 7H9 media containing [14C]-acetate (final concentration 0.5 μCi/ml; Perkin Elmer). Labeled cells were harvested by centrifugation at 5,000 x g for 3 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... Luciferase measurements were taken every other day beginning from day 2 (the day after the injection) – mice were anaesthetised (isoflurane inhalation) and subcutaneously injected with Xenolight D-luciferin substrate (PerkinElmer). Mice were imaged after 5min using the IVIS Lumina II (Caliper Life Sciences) ...
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bioRxiv - Biochemistry 2023Quote: ... was mixed in 20 µl of the cytoplasmic buffer with 2- to 10-fold excess of recombinant Sm proteins and each sample was supplemented with 2 µCi of 3H-labeled SAM (PerkinElmer) for overnight methylation at 32 °C ...
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bioRxiv - Immunology 2023Quote: ... Virus–monoclonal antibody-containing medium was then aspirated from cells and 50 or 100 µl of a 1:2 dilution of SteadyLite Plus (PerkinElmer) or BioGlo (Promega ...
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bioRxiv - Biochemistry 2023Quote: ... FLAG and HA were detected using the LI-COR system and HRP-conjugated GAPDH primary antibody was incubated with blots for 2 hours at room temperature and detected with Western Lightning Plus-ECL (PerkinElmer) using a Bio-Rad Chemidoc System.
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bioRxiv - Cell Biology 2024Quote: ... The plate was scanned and images were acquired in real-time every 2 hours from 0 to 24 h post-treatment by Opera Phenix High Content Screening System by PerkinElmer, Inc ...
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bioRxiv - Biochemistry 2024Quote: ... 10 µg RNA samples were separated on 2% glyoxal agarose gels and transferred onto nylon membranes (GeneScreen Plus, Perkin Elmer). Complementary [32P]-labeled probes were synthesized by linear PCR and hybridized at 65°C overnight in hybridization solution (0.5 M NaP pH = 7.0 ...
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bioRxiv - Cancer Biology 2024Quote: ... mice are anestatized with a flow rate of 1-2% and imaged using IVIS Spectrum In Vivo Imaging System (PerkinElmer). Mice were sacrificed when tumor size reached 2000 mm3.
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bioRxiv - Molecular Biology 2024Quote: ... 1-2 μg of total RNA was used to isolate poly-A RNA with NEXTFLEX Poly(A) beads 2.0 (Perkin Elmer), while libraries were prepared using the NEXTFLEX Rapid Directional RNA-seq Kit 2.0 (PerkinElmer) ...
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bioRxiv - Microbiology 2024Quote: ... plates were incubated in the dark at 37°C + 5% CO2 before their fluorescent signal was measured at 2 hours post-incubation at 485/535nm using the Victor3V microplate reader (PerkinElmer). Background signal from unstimulated cells were subtracted from zymosan stimulated samples for analysis ...
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bioRxiv - Bioengineering 2024Quote: ... was injected subcutaneously (Figure 2) or intraperitoneally (Figure 4) into mice and bioluminescence imaging was performed using IVIS Lumina series III (PerkinElmer). During the measurement ...
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bioRxiv - Biochemistry 2024Quote: ... then incubated with Ham’s F-10 media supplemented with 1.5 mCi/well myo[2-3H(N)] inositol (Perkin Elmer/Revvity) for 16-18 h ...
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bioRxiv - Biochemistry 2024Quote: ... Radioactivity was measured (counts per minute, CPM) using a scintillation counter (MicroBeta 2 Plate Counter) with a scintillation cocktail (OptiPhase SuperMix, PerkinElmer). Data were analyzed via nonlinear regression in GraphPad Prism v10.
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bioRxiv - Physiology 2023Quote: ... and 125 uM of 14C-labeled palmitic acid (PerkinElmer). 14C-labeled products were separated from the reactions by extraction with chloroform/methanol as previously described (32) ...
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bioRxiv - Zoology 2022Quote: ... Volatile fatty acids were analysed by gas chromatography using crotonic acid as internal standard on a Perkin Elmer Clarus 580 GC (Perkin Elmer, Courtaboeuf, France) equipped with a Stabilwax-DA column (30 m by 0.53 mm i.d. ...
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bioRxiv - Biophysics 2022Quote: ... Fluorescence polarization measurements were carried out in black 96-well plates measured on a Wallac Victor 2 Plate Reader (Perkin Elmer). For all measurements peptide was held at concentrations of 50 nm (or 100nM ...
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bioRxiv - Biophysics 2022Quote: ... Samples were dried for at least 30 mins at 95 °C to remove unfixed 14C before re-suspending the fixed 14C pellets with ultra-pure water and adding 2 mL of scintillation cocktail (Ultima Gold XR, PerkinElmer, US). Radioactivity measurements were then performed using a scintillation counter (Tri-Carb ...
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bioRxiv - Cell Biology 2021Quote: ... 500nl of 2 µM siRNAs (20nM final concentration) were distributed on 6 different 384-well imaging plates (CellCarrier Ultra, Perkin Elmer), as described in Table 3 ...
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bioRxiv - Microbiology 2022Quote: ... Cytotoxicity was measured by adding resazurin [alamar blue (ThermoFisher)] diluted to 1x in DMEM according to the manufacturor’s protocol and incubated on cells for 2-4hr and reading fluorescence on a Victor 1420 Multilabel plate reader (Perkin Elmer) with excitation at 560 nM and emission at 590 nM.
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bioRxiv - Neuroscience 2021Quote: ... cells were washed and incubated at 37 °C in phosphate buffered saline (PBS) containing 2 μCi/ml [3H]-glycine (1.6 TBq/mmol; PerkinElmer Life Sciences) isotopically diluted to reach 10 μM final glycine concentration ...
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bioRxiv - Neuroscience 2020Quote: ... cells were incubated in a solution containing an isotopic dilution of 2 μCi/ml [3H] glycine (1.6 TBq/mmol; PerkinElmer Life Sciences) in PBS ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and the amount of tritiated acetate in the organic phase was measured by liquid scintillation counting for 2 min (Tri-Carb 2900TR, Perkin Elmer). Each sample was assayed in triplicate and counts were corrected for background by subtraction of counts obtained using whole blood samples devoid of cholinesterase activity and treated in a similar fashion ...
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bioRxiv - Physiology 2021Quote: ... a longer time was required to achieve steady state) a single bolus of 12 μCi of [1-14C]-2-deoxy-D-glucose (Perkin Elmer) in 96 μl of saline was administered ...
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bioRxiv - Immunology 2021Quote: ... brain and testis tissue samples from hACE2 transgenic mice challenged intranasally with SARS-CoV-2-nLuc (1 x 105 FFU; 6 dpi) were imaged after necropsy using bioluminescence imaging (IVIS, Perkin Elmer), pruned to isolate regions with high nLuc activity and immediately pre-fixed with 3 % glutaraldehyde ...