Labshake search
Citations for PerkinElmer :
2001 - 2050 of 3095 citations for Cortisone Chemiluminescent ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... or 1 μg of GST-Raf13 as the Pak1 substrate with a total volume of 5 μL in a 384-well plate (PerkinElmer, Waltham, MA, USA) at 30°C for 1 h ...
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bioRxiv - Microbiology 2022Quote: ... blood plasma collected from the tip of the tail vein was diluted with equal volume of PBS (pH 7.4) and analyzed for DX-4000-FITC concentration with a fluorescence spectrophotometer (HTS-7000 Plus-plate-reader; Perkin Elmer, Wellesley, Massachusetts, USA) at an excitation wavelength of 485 nm and emission wavelength of 535 nm ...
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bioRxiv - Genomics 2020Quote: Custom EvaluPlate Attachment Surfaces plates (BioMedTech Laboratories, Inc.) were generated by coating wells of CellCarrier-384 Ultra imaging microplates (PerkinElmer, cat. no. 6057300) with 8 different cell culture matrices ...
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bioRxiv - Physiology 2020Quote: ... sperm was exposed to exosomes or drugs and fluorescence intensity was assessed by the EnSpire® Multimode Plate Reader (PerkinElmer, Waltham, MA, USA) with excitation and emission wavelengths of 488 and 525 nm ...
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bioRxiv - Neuroscience 2021Quote: NPCs were seeded onto poly-L-ornithine/laminin-coated 6-well and cyclic olefin co-polymer-based 96-well Cell Carrier Ultra microtiter plates (Perkin Elmer, Waltham, MA) at 1×105 and 2×103 cells/well ...
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bioRxiv - Molecular Biology 2021Quote: ... The fluorescence intensity and background signals of different concentrations of the ssDNA-FQ reporters before and after the cleavage were further detected by the EnSpire Multimode Plate Reader (EnSpire, PerkinElmer, Waltham, MA, USA). Fluorescence excitation and emission wavelengths were set to 490–750 and 520– 770 nm ...
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bioRxiv - Immunology 2020Quote: ... luciferase expressing target cells were transferred to a white round-bottomed 96-well plate in the presence of Xenolight D-Luciferin (75 μg/mL; Perkin Elmer, Oslo, Norway). CAR-expressing T cells were subsequently added in the mix and cells were cultured in an incubator (37 °C in 5% CO2 ...
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bioRxiv - Cancer Biology 2024Quote: ... and the luminescence signals per well were detected with Envision Xcite 2104 plate reader with ultrasensitive luminescence detector (revvity, previously Perkin Elmer, MA, USA). Eight wells per plate without library drug served as negative controls ...
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bioRxiv - Immunology 2024Quote: ... After an additional 2 hours blood was collected and serum FITC-dextran levels were measured with a Victor V multiparameter plate reader (Perkin Elmer, Waltham, MA). Quantification of serum FITC-dextran levels was performed with the WorkOut 2.5 software by extrapolation against know concentrations of FITC-dextran standard.
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bioRxiv - Immunology 2024Quote: ... Luciferase activity was quantified after 48–72 h by the addition of Steadylite Plus chemiluminescence substrate and analysis on a Perkin Elmer EnSight multimode plate reader (Perkin Elmer, Beaconsfield, UK). Antibody titre was then estimated by interpolating the point at which infectivity had been reduced to 50% of the value for the ‘no serum’ control samples.
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bioRxiv - Physiology 2023Quote: ... and STOP solution was added before the absorbance was measured at 450 nm using EnSpire™ Multimode Plate Reader (PerkinElmer, Waltham, MA, USA).
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and the fluorescence emission (F) of Venus and CFP were measured (535/25 and 470/24 nm, respectively) in an EnVision plate reader (PerkinElmer, USA, NY, Melville). Venus emission was corrected for spillover of CFP emission into the Venus channel (72.3%) ...
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bioRxiv - Synthetic Biology 2023Quote: ... cultures were pelleted and for each test well 100 μL of supernatant added to 100 μL of fresh M9 media in a new 96 well plate and absorbance measured at 400 nm using an EnSpire 2300 Multilabel Reader (PerkinElmer, Waltham, MA, USA). For mass spectrometry analysis ...
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bioRxiv - Cancer Biology 2023Quote: ... culture plates were incubated for 3 days after which they were imaged with an Operetta CLS high-content analysis system (Perkin Elmer, LIVE configuration) with the incubation chamber set to 37 °C and 5% CO2 ...
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bioRxiv - Immunology 2023Quote: N/TERT cells or human primary keratinocytes of various genotypes were seeded at a cell density of 10,000 cells/well in a black 96-well plate (PerkinElmer, CellCarrier-96 Ultra, #6055300) or a cell density of 80,000 cells/well in black 24-well plates (Cellvis ...
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bioRxiv - Cell Biology 2023Quote: ... the cell plates were immediately started for live cell imaging on an automated spinning-disk confocal high-content imaging system (Perkin Elmer, Opera Phenix) using a 40X water immersion objective lens under 5% CO2 and 37 °C condition ...
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bioRxiv - Plant Biology 2023Quote: ... The absorbance of 100 µl of the reaction solutions at 820 nm was measured using a ARVOX3 plate reader (PerkinElmer, Waltham, MA, USA) or a BioTek Synergy H1 (BioTek Instruments Inc. ...
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bioRxiv - Immunology 2023Quote: ... luciferase activity was quantified by the addition of Steadylite Plus chemiluminescence substrate and analysis on a Perkin Elmer EnSight multimode plate reader (Perkin Elmer, Beaconsfield, UK). Antibody titre was then estimated by interpolating the point at which infectivity had been reduced to 50% of the value for the no serum control samples.
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bioRxiv - Cancer Biology 2023Quote: ... cell fluorescence was detected under the KEYENCE BZ-X800 fluorescence microscope and measured by the Multilabel Plate Reader ARVO X5 (PerkinElmer, Waltham, MA, USA) at Em 440 nm and 535 nm in response to Ex at 340 and 380 nm ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... cells were seeded at 25000 cells per well in culture medium into white bottom opaque 96 well plates (Perkin Elmer cat no. 5680). Plates were incubated at 37 °C ...
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bioRxiv - Cell Biology 2023Quote: ... Colorimetric or fluorimetric quantifications of the respective readouts were done in a multiwell plate reader (Wallac Victor 3 V, PerkinElmer, Waltham, MA, USA) and conducted in white 96-well plates.
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bioRxiv - Microbiology 2024Quote: ... Fluorescence was measured at 485 nm excitation and 528 nm emission in a VICTOR® Nivo™ plate reader (Perkin Elmer, Waltham, USA) and the IC50s values were then calculated using Prism 7 (GraphPad ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... diluted 20-fold in 1X HBSS for 20 minutes at room temperature and total luminescence read using the Microbeta2 plate reader (Perkin Elmer, Waltham, MA) detecting total light counts in one second in each well ...
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bioRxiv - Immunology 2020Quote: ... Staining of neutrophils (specifically myeloperoxidase activity) in LWT wild-type larvae was completed using TSA staining kit (Cy5-TSA Cyanine Kit, PerkinElmer, NEL705A001KT). Fish were incubated in a 1:100 ratio of Cy5-TSA:amplification diluent at 28°C for 10 minutes in the dark ...
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bioRxiv - Neuroscience 2022Quote: ... siScrambled or siAbdc1/2 Astrocytes were incubated overnight with DMEM without glucose supplemented with 1% FBS containing 1 μCi/mL 14C-Acetate ([1-14C] acetate, 58 mCi/mmol, PerkinElmer) and or α-cyclo or 50 μM C26:0 ...
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bioRxiv - Cell Biology 2021Quote: ... hand-picked human islets were dispersed in accutase for 10 min at 37°C and plated in 96-well plates (Perkin Elmer Inc., Waltham, MA) treated with Poly-D-Lysine Hydrobromide (Sigma-Aldrich ...
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bioRxiv - Immunology 2022Quote: N-T TERT cells of various genotypes were seeded at a cell density of 10000 cells/well in a black 96 well plate (PerkinElmer, CellCarrier-96 Ultra, #6055300). The next day cells were treated with chemicals and stained with 1μg/ml dilution of propidium iodide (PI ...
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bioRxiv - Synthetic Biology 2019Quote: Transient transfections had approximately 75,000 in 500 μL of complete supplemented DMEM media added to each well of a VisiPlate-24 well black plate (PerkinElmer, catalog number: 1450-605) and were first grown for 24 hours ...
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bioRxiv - Genetics 2019Quote: ... The increased fluorescence was measured (using an excitation between 530-560 nm and an emission at 590 nm) using an EnVision® multimode plate reader (Perkin Elmer, Llantrisant, UK). The biological activity was calculated for each protein from proliferation curves using GraphPad Prism version 6.0.
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bioRxiv - Neuroscience 2020Quote: ... and the fluorescent emission was read at 665 nm and 620 nm in an EnVision® Xcite Multilabel Plate Reader (PerkinElmer, Waltham, MA, USA). IP1 content was calculated based on a standard curve covering concentrations from 2.7–11,000 nM according to the IP-ONE kit protocol.
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bioRxiv - Neuroscience 2019Quote: ... Fluorescence emission intensity of GCaMP6s was recorded at 515 nm upon excitation at 488 nm on an EnSpire® Multimode Plate Reader (PerkinElmer, Boston, MO, USA) for 330 s every 5 s and 100 flashes per well ...
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bioRxiv - Biochemistry 2021Quote: ... Plates were incubated at room temperature for 2 h before measurement of the fluorescence using an EnVision Multimode Plate (Reader PerkinElmer Life and Analytical Sciences). All values were converted to cAMP concentration using cAMP standard curve performed parallel and data were subsequently normalized to the response of 100 μM forskolin in each cell line ...
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bioRxiv - Biochemistry 2021Quote: ... Ub-AMC cleavage was monitored by measuring fluorescence in real time for at least 30 min at 365 nm excitation and 460 nm emission with an EnVision plate reader (Perkin Elmer, ICCB Facility, HMS). The initial kinetics observed within the linear range was used for plotting ...
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bioRxiv - Developmental Biology 2022Quote: ... 45 μL of the resuspension was distributed to six wells of a 96-well OptiPlate black-white plate (Perkin Elmer Life Sciences, Waltham, MA). Cells were then incubated for 10 mins with 10 μL coelenterazine-h (final concentration 5 μM ...
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bioRxiv - Microbiology 2022Quote: ... and 200 μl of parasites were applied to each well of HFF cells grown in 96-well plates with transparent bottoms (Perkin Elmer cat. no. 6055300). Plates were centrifuged at 290 g for 5 minutes ...
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The CB1 receptor interacts with cereblon and drives cereblon deficiency-associated memory shortfallsbioRxiv - Neuroscience 2023Quote: Global CB1R signalling was determined by label-free technology as previously described (Costas-Insua et al, 2021; Maroto et al, 2023) by using an EnSpire® Multimode Plate Reader (PerkinElmer, Waltham, MA, USA). Briefly ...
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bioRxiv - Genomics 2023Quote: ... was measured in triplicate and averaged at 405 nm for 45-60 minutes at 25°C on a Wallac Victor 2 1420 Multilabel Counter Plate Reader (Perkin Elmer, Waltham, MA, USA). Citrate synthase activity was standardized against the protein concentration of each sample using the Bovine Serum Albumin Assay (500-0201 ...
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bioRxiv - Immunology 2023Quote: ... A chemiluminescence assay was conducted for continuous measurement of chemiluminescence counts per second (cps) at 37°C in an Envision multimode plate reader (PerkinElmer Life Sciences, Waltham, MA), recording measurements at 1 min intervals.
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The tumour microenvironment shapes dendritic cell plasticity in a human organotypic melanoma culturebioRxiv - Immunology 2019Quote: ... with the Opal seven-color IHC Kit (NEL797B001KT; PerkinElmer) containing the fluorophores ...
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bioRxiv - Genomics 2020Quote: A NEXTflex® Rapid Directional qRNA-Seq Kit (PerkinElmer) was used to sequence the whole ASFV transcriptome via a conventional short-read approach ...
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bioRxiv - Genomics 2021Quote: ... DNA was extracted using the Chemagen kit (Perkin Elmer), in batches by cohort ...
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bioRxiv - Biochemistry 2021Quote: ... cAMP signal was detected by LANCE cAMP kit (PerkinElmer) according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... Tyramide biotin amplification (TSA Plus Biotin Kit, Perkin Elmer) was performed for 10 minutes ...
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bioRxiv - Developmental Biology 2020Quote: ... The TSA-Kit secondary antibody system (Perkin Elmer, NEL763E001KT) was utilized with Lef1 immunostaining.
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bioRxiv - Genomics 2020Quote: ... The NEXTflex® Rapid Directional qRNA-Seq Kit (PerkinElmer) was used for library preparation from the rRNA-depleted sample ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... ATPLite Luminescence Assay kit was purchased from PerkinElmer (6016949). Cell Staining Buffer (420201 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... ATPLite Luminescence Assay kit was purchased from PerkinElmer (6016949). Cell Staining Buffer (420201 ...
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bioRxiv - Molecular Biology 2021Quote: ... using the BigDye Terminator cycle sequencing kit (Perkin Elmer, Applied Biosystems Division ...
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bioRxiv - Neuroscience 2021Quote: ... (TSA Fluorescence Kit, Catalog # NEL760001KT; PerkinElmer, Inc., MA, USA) was added for 30 min at 40°C ...
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bioRxiv - Neuroscience 2020Quote: ... and developed using the ECL detection kit (PerkinElmer, NEL105001EA).