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Citations for PerkinElmer :
151 - 200 of 207 citations for Sulfur Free Cobalt Metallo Organic Standard Co @ 5000 µg g in Hydrocarbon Oil since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... The metals were analysed based on APHA standards (method 2320B, Waltham, MA, USA) with atomic absorption (ICP-OES, Perkin Elmer, model Optima 8300, MA, USA). Spectrophotometric determination of ammonium ...
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bioRxiv - Molecular Biology 2024Quote: ... anti-FLAG donor and protein G coated acceptor beads (10 ng/μl final concentration; AS103D and AL102C, PerkinElmer). For the competitive assay with CD81-GFP and antiHER2 EVs ...
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bioRxiv - Cancer Biology 2023Quote: ... mice were injected intraperitoneally with 15 mg/mL (at 5 µL/g BW) of D-Luciferin (Perkin Elmer) and imaged ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... The supernatant absorbance was measured at 630 nm on a label-free EnSight Multimode Plate Reader (Perkin Elmer, USA). The values obtained from flies fed with non-labelled food were used as control and subtracted from experimental readings ...
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Secretin receptor as a target in gastrointestinal cancer: expression analysis and ligand developmentbioRxiv - Cancer Biology 2022Quote: ... to a concentration of 200 μM with an addition of 0.5 mCi carrier-free 125I-NaI in 2 μl of carrier solution (PerkinElmer). Iodination was initiated by addition of 4 μl chloramin-T solution (SigmaAldrich ...
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bioRxiv - Cell Biology 2022Quote: ... cells were incubated for 1h in methionine (Met)- and cysteine (Cys)-free DMEM supplemented with 250μCi of 35S-Met/Cys radiolabel (PerkinElmer) per condition at normoxia ...
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bioRxiv - Cell Biology 2020Quote: Fully differentiated C2C12 MS2-CAS myotubes were starved in serum-and glucose-free DMEM for 2 hrs before a 3-hr incubation in low-glucose DMEM/fatty acid-free BSA 0.2% supplemented with radiolabeled glucose (Glucose, D-[3-3H]; 1 μCi/ml; PerkinElmer). Then ...
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bioRxiv - Microbiology 2022Quote: ... 10 μCi of either [1,2-3H(N)] free cholesterol or [1,2-3H(N)]cholesteryl oleate (Perkin Elmer, Boston, MA) were mixed with absolute ethanol ...
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bioRxiv - Immunology 2023Quote: ... 5x104 of cells were resuspended in 150ul of phenol-free RPMI media and spiked with 20uM of ATP (PerkinElmer). After 30min of incubation at 37°C supernatants were collected ...
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bioRxiv - Immunology 2023Quote: ... 5x104 of cells were resuspended in 150ul of phenol free RPMI media and spiked with 20uM of ATP (PerkinElmer). After 10min of incubation at 37°C supernatants were collected and concentration of ATP/sample was measured using ATPlite Luminescence Assay System (PerkinElmer ...
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bioRxiv - Biochemistry 2024Quote: ... 10 µg RNA samples were separated on 2% glyoxal agarose gels and transferred onto nylon membranes (GeneScreen Plus, Perkin Elmer). Complementary [32P]-labeled probes were synthesized by linear PCR and hybridized at 65°C overnight in hybridization solution (0.5 M NaP pH = 7.0 ...
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bioRxiv - Cell Biology 2024Quote: ... 10 µg/mL of SYNs were added to hMG plated on a CellCarrier™-96 Ultra Microplate (Perkin Elmer®) and imaged using 20x objective of a Zeiss SP05 confocal microscopy ...
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bioRxiv - Plant Biology 2020Quote: ... 1 μg of peptides and 1 μg of GCN5-ADA2 complex were incubated in 25 μl HAT buffer containing 0.625 μCi 3H-Acetyl-CoA (PerkinElmer) at 23 °C for 2 hours ...
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bioRxiv - Neuroscience 2024Quote: ... we administered four intraperitoneal injections of 3H-Thy at 2 hrs intervals to adult mice (1.67 μL/g body weight, specific activity 5 Ci/mmol; PerkinElmer) with subsequent perfusion 2 hrs after the last injection (n = 3) ...
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bioRxiv - Genomics 2024Quote: ... and the run-on conducted in the presence of 25 μM biotin-11-A/C/G/UTP (Perkin Elmer cat nrs ...
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bioRxiv - Immunology 2024Quote: ... along with an intravenous injection of 1.5 × 107 heat-killed Escherichia coli (E. coli)/g body weight (Perkin Elmer, XEN 14 derived from the parental strain E ...
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bioRxiv - Neuroscience 2020Quote: ... PV staining was used to identify the localization and extension of the BLA and the borders were manually drawn by a neuroscientist of Lab-Inns1 using the free shape tool of the Improvision Openlab software (PerkinElmer). Boundaries were projected to the respective cFOS-immunoreactive image and cFOS-positive nuclei were counted manually inside that area by the expert of Lab-Inns1 ...
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bioRxiv - Microbiology 2022Quote: ... and at indicated times were washed and incubated for 30 mins in methionine-free DMEM before adding 50μCi of L-[35S]-methionine (Perkin Elmer) for a further 30 min ...
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bioRxiv - Systems Biology 2023Quote: ... Free amino acids were determined with the EZ:FAAST amino acid analysis kit of Phenomenex on a GC-FID (Perkin Elmer) according to supplier instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 µCi 3H-SAM and ∼1 µg of enzyme) for 1 hour at 30° C following which they are transferred to FlashPlates (Perkin Elmer) and incubated for 30 min for peptide capture ...
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bioRxiv - Molecular Biology 2022Quote: ... Then for the 6xHIS-tagged HAT module a 10 µL mixture of 5 µg/mL AlphaLISA Nickel chelate acceptors beads (PerkinElmer #AL108) and 10 µg/mL AlphaScreen donor beads (PerkinElmer #6760002) ...
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bioRxiv - Cell Biology 2024Quote: ... together with 10 µl Tem1 coated donor beads and 10 µl 50 µg/µl AlphaLISA Anti-GFP Acceptor Beads (PerkinElmer AL133C) diluted in assay buffer ...
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bioRxiv - Microbiology 2021Quote: ... 1.37 μg/mL of RBD and 5 μg/mL of Ecto) and incubated overnight in protein-binding 96-well plates (PerkinElmer). The next day ...
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bioRxiv - Cell Biology 2024Quote: Plasma samples were collected in two 10-ml EDTA and a 4.5-ml tube with sodium citrate and centrifuged at 2,000×g for 10 min as previously described (Durat et al, 2014) and fractionated by automation (Janus robotic liquid handlers; PerkinElmer). Typically ...
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bioRxiv - Cancer Biology 2023Quote: ... mice were given 10 μL/g of D-luciferin (15 mg/mL) (Gold Bio) intraperitoneally and imaged using the IVIS Spectrum imager (PerkinElmer). Luciferase signal was quantified 15 min after injection ...
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bioRxiv - Cell Biology 2023Quote: ... 50 µ g of lysate was used for the assessment of ATP concentration using an ATP Determination Kit (ATP lite assay, PerkinElmer) as described earlier (Miyoshi et al. ...
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bioRxiv - Bioengineering 2023Quote: ... The plasma was obtained by centrifugation at 500 g for 5 min and measured for MFI using IVIS (PerkinElmer, USA).
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bioRxiv - Microbiology 2020Quote: ... cells were metabolically pulse labeled with methionine- and cystine-free DMEM supplemented with 70 μCi/ml 35S-labelled Met/Cys (EasyTag EXPRESS35S, Perkin Elmer). Total protein was boiled in SDS sample buffer and then either fractionated by SDS PAGE and exposed to film or precipitated in 10% Trichloroacetic acid (TCA ...
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bioRxiv - Cancer Biology 2021Quote: ... with 15 µl of sodium phosphate buffer (0.5 M, pH 7.6) was mixed with 37 MBq carrier-free Na125I (NEZ033L010MC, Perkin Elmer, Waltham, US). 4 μl chloramine T (1 mg/ml ...
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bioRxiv - Microbiology 2023Quote: ... cells were washed twice with methionine-free DMEM and labeling media was prepared by adding 75 uCi of [35S] methionine per mL (PerkinElmer, NEG709A) of methionine-free medium (Gibco) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... with 15 μL of sodium phosphate buffer (0.5 M, pH 7.6) was mixed with 37 MBq carrier-free Na125I (NEZ033L010MC, Perkin Elmer, Waltham, US). 4 μl chloramine T (1 mg/mL ...
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bioRxiv - Biochemistry 2024Quote: ... Fluorescence intensity of this protein-free promoter sample was continuously measured for 120 s with a LS-55 fluorescence spectrofluorometer (Perkin Elmer) at 24°C ...
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bioRxiv - Molecular Biology 2023Quote: ... GLuc production was measured as a surrogate for IFN-β promoter activation by adding 25 µl coelenterazine-solution (1 µg/ml in H2O) was to 25 µl supernatant.reader (Perkin Elmer, Waltham, USA) and luminescence was measured ...
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bioRxiv - Systems Biology 2024Quote: ... The NPCs were cultured in a 12-well dish to confluency in NPC media and then dissociated using Accutase for 5 minutes at 37°C to single cell resolution and plated on 5 µg/mL human laminin and 5µg/mL fibronectin-coated 384 well plates (Perkin Elmer Cat #6057308) at 12,000 cells/well in NPC media and 10 µM ROCKi ...
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bioRxiv - Molecular Biology 2021Quote: ... Solutions were then cleared by centrifugation at 4000 g for 10 min and the supernatant analyzed in a black 96 well microtiter plate using an EnSpire (PerkinElmer, USA) microplate reader with an Excitation/Emission wavelength of 630 nm/ 660 nm (with 9 nm band-passes around the maximum) ...
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bioRxiv - Microbiology 2020Quote: ... streptavidin donor beads were added (20 μg/ml) and the mixtures were incubated for 30 mins and were then measured with the Ensight multimode reader (Perkin Elmer) using excitation wavelength of 680 nm with donor beads and measurement of emission wavelength of 615 nm from AlphaLISA anti-HIS acceptor beads ...
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bioRxiv - Microbiology 2020Quote: ... rabbit reticulocyte lysate was incubated with 1μg of the appropriate expression plasmid and 2mL of [35S] L-methionine (1,000 Ci/mMol at 10mCi/mL) (Perkin Elmer, NEG709A) for 90 minutes at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... then centrifuged at 61 g for 10 seconds and the data were acquired in a Victor X 5 plate reader (Perkin Elmer) with an excitation wavelength of 480 nm and an emission wavelength of 535 nm ...
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bioRxiv - Molecular Biology 2023Quote: ... incubation with a 32P-labelled oligodeoxyribonucleotide probe complementary to the target RNAs (32P-labelled using T4 polynucleotide kinase and [g 32P]-ATP [Perkin Elmer]) overnight in 37°C in SES1 buffer ...
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bioRxiv - Synthetic Biology 2023Quote: ... female NMRI mice with a bodyweight of around 20 g were administered intraperitoneally with 150 mg/kg D-luciferin (PerkinElmer, 122799). After 5 min ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: The recruitment of β-arrestin2 by MOR agonists after G protein activation was assessed using the Cisbio HTRF-based β-arrestin2 recruitment assays (Cisbio Assays, PerkinElmer), which monitored the interaction between β-arrestin2 and AP2 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: WT or ARE-LUC expressing HepG2 were grown in either 24-well or 96-well plates to approximately 70% confluence when the medium was changed to serum-free medium supplemented with 100 nM 75Se (University of Missouri Research Reactor; specific activity of ∼500 Ci/g) or 50 uCi/ml 35S Met/Cys (Perkin Elmer). For drug or control treatments ...
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bioRxiv - Cancer Biology 2024Quote: Luminescence imaging was carried out on days indicated using an IVIS luminescence imaging system 10 min after mice were injected with 10 µl/g of body weight 15 mg/mL in vivo grade D-Luciferin (PerkinElmer, 122799). Mice were sacrificed when reaching AAALAC-defined endpoints by CO2 asphyxiation followed by cervical dislocation ...
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bioRxiv - Plant Biology 2021Quote: ... Seedlings were metabolically labelled with radioactive phosphate by incubating them overnight for ~16-20 hrs with 10 μL (5-10 μCi) carrier-free 32P-PO43- (32Pi; PerkinElmer, The Netherlands) in labelling buffer ...
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bioRxiv - Cell Biology 2021Quote: RAW cells were incubated for 24 h with inositol-free DMEM (MP Biomedica, CA) containing 10 μCi/ml myo-[2-3H(N)] inositol (Perkin Elmer, MA), 1X insulin-transferrin-selenium-ethanolamine (Gibco) ...
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bioRxiv - Cell Biology 2021Quote: ... media was removed and replaced with serum-free Modified Eagles Medium (MEM; Mediatech) containing 1 µCi/ml of 3H-myo-inositol (PerkinElmer Life Sciences). Forty-eight hrs post-infection ...
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bioRxiv - Biochemistry 2021Quote: ... metabolic labeling was started by replacing the medium with phosphate-free DMEM containing 10% dialyzed FBS and 1mCi/mL 32P orthophosphate (PerkinElmer, Waltham, MA). After labeling for 8 hours ...
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bioRxiv - Cell Biology 2024Quote: ... transfected cells were harvested using phenol red-free DMEM and seeded at a density of 1.0 × 104 cells/well onto CellCarrierTM-96 Ultra microplates (Perkin Elmer, Waltham, MA). Then ...
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bioRxiv - Cancer Biology 2024Quote: ... Mice were injected IP with 10 µL/g of body weight of 15 mg/mL in vivo grade D-Luciferin (luciferin; PerkinElmer, Waltham, MA), anesthetized with 2-3% isoflurane in an induction chamber ...
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bioRxiv - Cancer Biology 2024Quote: ... Equal amounts of cell lysate proteins (20-50 μg) or proteins eluted from agarose beads were separated on SDS-PAGE and transferred to polyvinylidene difluoride (PVDF) membranes (PerkinElmer Life Sciences). Membranes were blocked 1 h with 3% BSA/TBST and incubated with primary antibodies overnight at 4°C ...