Labshake search
Citations for PerkinElmer :
151 - 200 of 529 citations for Recombinant Human GPC3 protein His tagged R PE labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2020Quote: ... LH-I-9 was labeled with europium using a Delfia Eu-labeling kit (PerkinElmer). Anti-rabbit gamma globulin goat serum was prepared in our laboratory and purified by ammonium sulfate precipitation ...
-
bioRxiv - Developmental Biology 2020Quote: ... the Tyramide labeled with a fluorophore (Opal 520, Opal 570 or Opal 650, PerkinElmer) was added for a final 10 min ...
-
bioRxiv - Neuroscience 2020Quote: ... Labeled transcripts were visualized with Cy3 (TSA Cyanine 3 System, NEL744A001KT, PerkinElmer, Waltham, MA) and sections were counter-stained with DAPI ...
-
bioRxiv - Molecular Biology 2020Quote: The 5′-end of the RNAs were labeled with [gamma-32P] ATP (Perkin Elmer) and T4 polynucleotide kinase (ATP ...
-
bioRxiv - Neuroscience 2019Quote: ... The fluorescein-labeled probe was visualized by using the TSA Plus Fluorescein System (PerkinElmer); the DIG-labeled probe was visualized by using alkaline phosphatase-conjugated anti-DIG antibody (Roche Diagnostics ...
-
bioRxiv - Immunology 2020Quote: ... K562 or GSCs target cells were labeled with 51Cr (PerkinElmer Life Sciences, Boston, MA) at 50 μCi/5 × 105 cells for 2 hours ...
-
bioRxiv - Biochemistry 2021Quote: ... 35S-pulse-labeled for 10 - 30 min at 37°C (Tran35S label, Perkin Elmer) and chased in complete growth media ...
-
bioRxiv - Biochemistry 2020Quote: ... Radio-labeled nucleotides (α32P-ATP, γ32P-GTP, 35S-GTPγS) were purchased from Perkin Elmer.
-
bioRxiv - Microbiology 2021Quote: ... It was labeled with [γ-32 P] dATP-6000 Ci/mmol (PerkinElmer Life Sciences) using T4 polynucleotide kinase ...
-
bioRxiv - Microbiology 2020Quote: ... cells were pulse labeled with 30 μCi ml−1 [35S] methionine (Perkin Elmer #NEG009T) and 30 μCi ml−1 [35S] cysteine (Perkin Elmer #NEG022T ...
-
bioRxiv - Molecular Biology 2019Quote: ... Primers were labeled with 32P gamma-ATP (6000 Ci/ mmol, 25 mM, Perkin Elmer) with PNK (NEB ...
-
bioRxiv - Immunology 2020Quote: Metabolic labeling was performed using S35-labeled Cysteine/Methionine (Perkin Elmer, Waltham, MA, USA). Cells were seeded to 60%-70% confluence in 6-well plates ...
-
bioRxiv - Molecular Biology 2023Quote: ... purified mitochondria were labeled with 30 μCi of alpha-32P UTP (Perkin Elmer BLU007H250UC) in transcription buffer (10 mM Tris pH 7.5 ...
-
bioRxiv - Microbiology 2023Quote: ... Secondary antibodies used were 1:10,000 of HRP-labeled α-mouse (for Flag) (PerkinElmer) or α-rabbit (PerkinElmer ...
-
bioRxiv - Cancer Biology 2023Quote: ... The probe was labeled on the 5′ end with [γ-32P] ATP (Perkin Elmer) using T4 polynucleotide kinase (NEB M0201 ...
-
bioRxiv - Genetics 2023Quote: ... and end-labeled with (γ-P32) adenosine triphosphate (MP Biomedicals or Perkin Elmer, #NEG002A250U) using T4 polynucleotide kinase (NEB ...
-
bioRxiv - Cancer Biology 2020Quote: ... Signals from luciferase-tagged cells were monitored at day 0 after injection and weekly by in vivo imaging using IVIS Lumina II (Perkin Elmer) for 5 weeks ...
-
bioRxiv - Molecular Biology 2022Quote: ... Then for the 6xHIS-tagged HAT module a 10 µL mixture of 5 µg/mL AlphaLISA Nickel chelate acceptors beads (PerkinElmer #AL108) and 10 µg/mL AlphaScreen donor beads (PerkinElmer #6760002) ...
-
bioRxiv - Cell Biology 2023Quote: ... 10 nM full-length 6x-His-tagged human G3BP1 produced in mammalian cells was added to the reaction mix containing test compound and 0.5 nM anti-His-Tb antibody (PerkinElmer). Assays were initiated by adding 4 nM FAM-labeled PEG6-USP10 24-mer peptide probe (FAM-PEG6-GALHSPQYIFGDFSPDEFNQFFVT ...
-
bioRxiv - Physiology 2022Quote: ... with 0.33mM R,S-[2-14C]mevalonic acid (53mCi/mmol; Perkin Elmer) hydrolysed from the lactone form ...
-
bioRxiv - Cell Biology 2020Quote: ... K562 cells (5×106/ml) were labeled with 200 μCi of 51Cr (PerkinElmer, Wellesley, MA). After labeling ...
-
bioRxiv - Molecular Biology 2021Quote: ... and 100 µL of 1 µg/mL of Europium-labeled Streptavidin (Perkin Elmer, 1244-360) in 0.1% BSA was added ...
-
bioRxiv - Developmental Biology 2020Quote: ... After detection of digoxigenin-labeled probes by deposition of TSA Plus fluorescein solution (Perkin Elmer), embryos were incubated with an AlexaFluor 647 goat anti-mouse IgG secondary antibody (Invitrogen ...
-
bioRxiv - Biochemistry 2020Quote: ... the 25-nt oligonucleotide was labeled at the 5’ end by γ-32P ATP (PerkinElmer) using T4 polynucleotide kinase (NEB).
-
bioRxiv - Immunology 2021Quote: ... The NIT-1 cells were then labeled with 1 μCi/well of 51Cr (Perkin Elmer) for 3 hours at 37°C and co-cultured with AI4 T cells at a E:T ratio of 20:1 for 16 hours ...
-
bioRxiv - Cancer Biology 2022Quote: ... The sections were labeled by using the Opal 7-Color fIHC Kit (PerkinElmer, Waltham, MA). The antibody fluorophores were grouped into a panel of 5 antibodies ...
-
bioRxiv - Microbiology 2023Quote: ... The template was labeled with the kit by adding 10 μCi alpha-CTP (Perkin Elmer) to the reaction for four hours at 37°C ...
-
bioRxiv - Genetics 2023Quote: ... Fluorescence was measured every 60 s using a multi-labeled microplate detector (Perkin Elmer Envision). Depolymerizing activities associated with the test proteins were identified by comparing the depolymerization curves ...
-
bioRxiv - Developmental Biology 2021Quote: ... RRID: AB_2340590) were used as secondary antibody and blots were detected using ECL detection (PerkinElmer (PE), NEL105001EA) ...
-
bioRxiv - Genetics 2020Quote: Mice were anesthetized with isoflurane and put into the Quantum FX system (PerkinElmer, PE Quantum FX) then scanned with X-ray ...
-
bioRxiv - Immunology 2022Quote: ... The quality and average fragment size of the final libraries were determined using DNA 1K/12K/Hi Sensitivity Assay LabChip and DNA High Sensitivity Reagent Kit (PerkinElmer). Libraries with 16 unique dual indexes were pooled and sequenced on a NextSeq 500 benchtop sequencer (Illumina ...
-
bioRxiv - Molecular Biology 2023Quote: ... The average fragment size of the final libraries was determined using DNA 1K/12K/Hi Sensitivity Assay LabChip (760517, PerkinElmer)) and DNA High Sensitivity Reagent Kit (CLS760672 ...
-
bioRxiv - Cell Biology 2022Quote: ... recombinant Ism1 or Igf1 with the addition of 0.5 μCi [35S]-methionine (#NEG009L005MC, PerkinElmer, Waltham, MA, USA) for 48h ...
-
bioRxiv - Immunology 2023Quote: ... using anti-human IFN-γ capture antibody (Pharmingen, 551221), biotinylated anti-human IFN-γ detection antibody (Pharmingen, 554550) and DELFIA Europium-labelled streptavidin (PerkinElmer), for fluorescent detection and quantification.
-
The conserved membrane-proximal domain of Sbh1/ Sec61β guides signal peptides into the Sec61 channelbioRxiv - Biochemistry 2024Quote: ... Proteins were labelled for 5 mins (for soluble proteins) or 15 mins (for membrane proteins) with 30 µCi of Express Protein Labelling Mix (Perkin Elmer) per sample at their restrictive temperature ...
-
bioRxiv - Bioengineering 2023Quote: ... Cardiomyocyte area and cardiomyocyte number were quantified by identifying cell membranes through staining tissue samples with fluorescent-tagged wheat germ agglutinin (WGA) and quantified using Mantra™ quantitative pathology workstation (PerKinElmer, Waltham, MA)17 ...
-
bioRxiv - Developmental Biology 2020Quote: ... Probes were labeled with digoxigenin and detected by deposition of TSA Plus fluorescein solution (Perkin Elmer). Stained embryos were washed in PBT and mounted in SlowFade Gold anti-fade reagent with DAPI (Molecular Probes ...
-
bioRxiv - Microbiology 2020Quote: ... 1 mL cells were labeled with 50 µCi of 32P orthophosphate (900 mCi/mmol; Perkin Elmer) or 2-3 generations before treatment or sampling ...
-
bioRxiv - Molecular Biology 2020Quote: ... 1/20th of the annealed substrates were 5′-end-labeled with [γ-32P]-ATP (Perkin Elmer) and T4 polynucleotide kinase (New England Biolabs) ...
-
bioRxiv - Developmental Biology 2020Quote: ... The restriction fragments of each region were then gel purified and labeled with [α32P]-dGTP (PerkinElmer) using the large fragment of DNA polymerase I (Invitrogen) ...
-
bioRxiv - Microbiology 2022Quote: ... Northern probes were 5’ 32P-end labeled with 0.3 mCi of y-32P ATP (Perkin Elmer) with 10 U of T4 Polynucleotide kinase (NEB ...
-
bioRxiv - Molecular Biology 2022Quote: ... blots were hybridized with RNA probes in vitro transcribed with 32P-labeled UTP (PerkinElmer Life Sciences). Radioactive signals were detected with a phosphorimager and signals were quantified with Quantity ONE software (Bio-Rad).
-
bioRxiv - Neuroscience 2023Quote: ... The DIG-labeled probes were amplified with horseradish peroxidase using Cy3.5 tyramide signal amplification kit (PerkinElmer) for 5 to 10 min.
-
bioRxiv - Developmental Biology 2023Quote: ... The annealed synthetic oligonucleotide DNA was labeled with [α-32P]dCTP (Perkin Elmer NEG013H250UC, 3000Ci/mmol) by Klenow polymerase ...
-
bioRxiv - Biophysics 2019Quote: AlphaScreen-based peptide recruitments assays were performed using streptavidin coated donor beads and anti-His-tag antibody coated AlphaLisa acceptor beads (Perkin Elmer). The assays were very sensitive to the distance of the N-terminus to the LXXLL NR-box motif and the use of a flexible Ahx linker ...
-
bioRxiv - Molecular Biology 2021Quote: ... metabolically labeled for 20 min with 35S-Met (50 μCi 35S-Met, 1175 Ci/mmol, Perkin Elmer) and then lysed in SDS-lysis buffer (25 mM Tris-HCl pH 7.5 ...
-
bioRxiv - Genomics 2020Quote: ... at 37°C for 1 hr and then labeled with 0.5μl of gamma 32P-ATP (PerkinElmer, BLU502A250UC) using 20U T4 polynucleotide kinase (NEB ...
-
bioRxiv - Cancer Biology 2020Quote: Imaging of fluorescently labeled tissue sections was performed utilizing Nuance-FX multispectral imaging system (Caliper LifeSciences, PerkinElmer), using full capabilities of its Tunable Liquid Crystal imaging module ...
-
bioRxiv - Biochemistry 2021Quote: ... 100 pmol of RNA were 5’-end labeled with [γ- 32P]-ATP (3000 Ci/mmol, Perkin Elmer) using T4 polynucleotide kinase (NEB ...
-
bioRxiv - Molecular Biology 2022Quote: ... 10mM sodium butyrate and 0.25 µCi/µl (4.9 Ci/mmol) of [3H]-labeled Acetyl-CoA (Perkin Elmer) or 0.15mM unlabeled Acetyl-CoA (Sigma) ...