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Citations for PerkinElmer :
151 - 200 of 215 citations for 8 Benzylthio 6 oxo octanoic Acid Methyl Ester d4 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... The proteins were separated on 7% tris-glycine or 8% tricine gels by SDS-PAGE and transferred to a PVDF membrane (Perkin Elmer). The proteins were revealed using a V5-monoclonal antibody (V5-mAb V2660 ...
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T-cell development and activation in humanized mice lacking mouse major histocompatibility complexesbioRxiv - Immunology 2024Quote: ... mice were analyzed at week 8 and 12 post-HCT by BLI analyses using the IVIS SpectrumCT (PerkinElmer, Waltham, MA, USA) as described (Theobald et al ...
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bioRxiv - Immunology 2023Quote: ... B cells were lysed in water by osmotic pressure and were transferred from 96 well plates to glass fiber filtermat (8×12 Filtermat A, GF/C, 100/pk, #1450-421, Perkin Elmer). The filters were rinsed with water and sealed in plastic bags with a scintillation cocktail (OptiScint® LLT ...
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bioRxiv - Cell Biology 2020Quote: Fully differentiated C2C12 MS2-CAS myotubes were starved in serum-and glucose-free DMEM for 2 hrs before a 3-hr incubation in low-glucose DMEM/fatty acid-free BSA 0.2% supplemented with radiolabeled glucose (Glucose, D-[3-3H]; 1 μCi/ml; PerkinElmer). Then ...
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bioRxiv - Microbiology 2021Quote: ... The reaction was then stopped with the addition of 0.16 N sulfuric acid and the absorbance measured at 450 nM on an EnSight multimode plate reader (PerkinElmer). Data were in-column normalized using cells containing serum only or no serum with buffer addition were used as 100% and 0% signal ...
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bioRxiv - Biochemistry 2021Quote: ... bound to albumin (1:1 molar ratio) or 0.1 μM [1-14C]oleic acid (2 μCi/dish Perkin Elmer) bound to albumin (1:1 molar ratio ...
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bioRxiv - Biochemistry 2021Quote: ... The radioactivity of the acid-insoluble pellet was measured through the addition of 50μL of MicroScint PS (Perkin Elmer), vortexing ...
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bioRxiv - Cell Biology 2021Quote: ... [9,10(n)-3H]oleic acid (OA) (54.6 Ci/mmol) and [14C]glycerol (250 μCi/mmol) were from Perkin Elmer. Inhibitors of DGAT1 (PF-04620110) ...
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bioRxiv - Biochemistry 2023Quote: ... 2-[1,2-3H(N)]-deoxy-D-glucose and [1,2-14C]-acetic acid were purchased from PerkinElmer (Waltham, MA, USA). PPARγ ...
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bioRxiv - Bioengineering 2023Quote: ... with primers from IDT (Leuven, Belgium) and analyzed on a LabChip® GX Touch Nucleic Acid Analyzer (Perkin Elmer). Plasmids with correct fragment size were amplified by PCR using Q5® High-Fidelity DNA polymerase (NEB ...
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bioRxiv - Microbiology 2024Quote: Overnight cultures were diluted to an OD600 of ∼0.05 in 10 mL LB Miller broth supplemented with 5 µCi/mL 32P ortho-phosphoric acid (PerkinElmer) and grown until reaching an OD600 of ∼0.6 ...
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bioRxiv - Microbiology 2021Quote: ... 1-3ng/μl of the PCR amplifier of the HPV L1 gene was mixed with 8 μL of ABI Prism BigDye Terminator Cycle Sequencing Ready Reaction kit version 1.1(Perkin Elmer Biosystems, USA), 2 pmol of primer ...
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bioRxiv - Neuroscience 2024Quote: HB(T)MVECs were incubated for 6 hours in EGM-2 containing 0.4 μCi/ml [5-3H]-D-glucose (PerkinElmer). Supernatant was transferred into glass vials containing perchloric acid and sealed with rubber stoppers ...
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bioRxiv - Biochemistry 2021Quote: Structural models of polyenoyl tetramic acids 3 and 4 were built and energy minimized using Chem3D v.16.0 (Perkin Elmer). The terminal region of polyenes ...
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bioRxiv - Cancer Biology 2020Quote: ... The cells were then washed twice with PBS and incubated with either H-H or L-L medium supplemented with 1μCi per well of L-[U-14C]-Aspartic acid (PerkinElmer, NEC268E050UC) for 40 h ...
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bioRxiv - Physiology 2020Quote: ... The integrity of resultant cDNA was assessed using a LabChip GX Touch 24 Nucleic Acid Analyzer as per manufacturer’s instructions (Perkin Elmer).
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bioRxiv - Immunology 2022Quote: ... Quality and quantity were checked using a Caliper LabChip GX Touch HT Nucleic Acid Analyzer (PerkinElmer, Waltham, MA 02452 USA). The PCR product library was quantitated and subjected to sequencing on an Illumina MiSeq sequencer (Illumina ...
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bioRxiv - Immunology 2022Quote: ... using the RNA Assay and DNA 5K/RNA/CZE HT Chip on a LabChip GX Touch HT Nucleic Acid Analyzer (PerkinElmer). TruSeq Stranded mRNA Library Prep kit (Illumina ...
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bioRxiv - Microbiology 2022Quote: ... DNA quantification and quality check was done using a LabChip GX Touch HT Nucleic Acid Analyzer (PerkinElmer, Waltham, MA, USA). Libraries were prepared as detailed on the Illumina® support website (http://support.illumina.com ...
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bioRxiv - Biochemistry 2023Quote: ... Protein biosynthesis rates were determined by measuring the incorporation of radioactive methionine in auranofin-treated and untreated samples after protein precipitation with 20 % (w/v) trichloro acetic acid using a scintillation counter (Tri-Carb 2800TR, PerkinElmer). 55 µg of protein from pulse-labeled samples for analytical gels and 300 µg of protein from unlabeled samples for preparative gels were rehydrated into IPG strips (24 cm ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 3.7 MBq/mL) and [2-14C] acetic acid (2.183 GBq/mmol, 37 MBq/mL) were purchased from PerkinElmer (Waltham, MA). For the ultra[performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS ...
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bioRxiv - Microbiology 2024Quote: ... Quality and quantity were checked using a Caliper LabChip GX Touch HT Nucleic Acid Analyzer (PerkinElmer, Waltham, MA 02452 USA). The PCR product library was quantitated and subjected to sequencing on an Illumina MiSeq sequencer (Illumina ...
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bioRxiv - Microbiology 2024Quote: ... Quality and quantity were checked using a Caliper LabChip GX Touch HT Nucleic Acid Analyzer (PerkinElmer, Waltham, MA 02452 USA). The PCR product library was quantitated and subjected to sequencing on an Illumina MiSeq sequencer (Illumina ...
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bioRxiv - Neuroscience 2023Quote: ... The saturation binding assay was conducted using an 8-point dose-dependent concentration of [3H] ketanserin (maximal concentration 20 nM) (Perkin Elmer, Waltham, MA) with brain crude membranes (25 μg membrane proteins ...
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bioRxiv - Cancer Biology 2024Quote: ... After 10 minutes the mice were imaged using the In Vivo Imaging System (IVIS) Spectrum connected to XGI-8 Anaesthesia System (PerkinElmer, Waltham, MA, USA). Bioluminescence was expressed as Total Flux per second.
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bioRxiv - Cancer Biology 2020Quote: ... the two replicates of the 6 h treatment time point for the H1299 cell line were acquired on an equivalent Opera Phenix imaging system (PerkinElmer). Quantitative analysis of the data was carried out using the metrics and analysis methods detailed below ...
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bioRxiv - Biochemistry 2021Quote: ... and 640 μM) were added along with 10 μM of non-radiolabeled UTP plus 6 mCi of [α32P]-UTP (PerkinElmer). Upon addition of nucleotides ...
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bioRxiv - Neuroscience 2020Quote: ... 3 and 6 hr later cells were labeled for 5 min with 1 mCi/ml 35S Protein Labeling Mix (PerkinElmer). Lysates from 25-ml culture triplicate samples were analyzed by SDS-PAGE and autoradiography.
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bioRxiv - Immunology 2020Quote: ... during the final 6 hours (murine MLR) or 15 hours (human MLR) of culture was measured by a TopCount (PerkinElmer).
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bioRxiv - Cancer Biology 2021Quote: 4T1 cells were cultured for 6 h in RPMI medium containing 0.4 μCi ml−1 [5-3H]D-glucose (Perkin Elmer) after which the supernatant was transferred into glass vials sealed with rubber stoppers ...
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bioRxiv - Microbiology 2022Quote: ... maintained at 200 °C and with N2 as the gas vector (flow rate of 6 mL·min-1) with a flame ionization detector (FID) maintained at 280 °C (PerkinElmer, USA)
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bioRxiv - Microbiology 2022Quote: ... OD600 and luminescence measurements were taken either 1-hour post-resuspension or over 6 hours starting at 0.5-hour post-resuspension using the Victor X3 2030 multilabel plate reader (Perkin Elmer). Luminescence was measured in counts per second (CPS ...
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bioRxiv - Immunology 2024Quote: ... Tumor engraftment was confirmed on day 6 via intraperitoneal injection of IVISbrite D-Luciferin Potassium Salt Bioluminescent Substrate (XenoLight, PerkinElmer), followed by bioluminescent imaging (BLI ...
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bioRxiv - Cancer Biology 2024Quote: ... Eµ-Myc lymphoma cells grown in 6-well plates were spiked with 0.2 μCi/mL [1-14C]-acetate (PerkinElmer; NEC084H001MC) for the final 4 h of the experimental period ...
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bioRxiv - Immunology 2024Quote: ... [3H]-thymidine was added at 0.5μl (∼0.5 μCi) per well and plates incubated for 6 h before cells were harvested to UniFilter plates (Perkin Elmer) using a Tomtec 96 Harvester ...
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bioRxiv - Microbiology 2023Quote: ... cells were cultured in 6-well plates and metabolically labeled (Easytag Express 35S-Met-Cys protein labeling mix (Perkin Elmer)) with 0.2 mCi/ml for various times ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were then treated for 10 minutes with indicated compounds before addition of 20 μCi of 32P-ortho-phosphoric acid (Perkin Elmer). Cells were incubated for 1 hour with media containing drugs and 32P-ortho-phosphoric acid ...
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bioRxiv - Cancer Biology 2022Quote: ... Solid tumor total nucleic acid was extracted from these tumor regions on adjacent FFPE tissue sections using Chemagic 360 sample-specific extraction kits (Perkin Elmer) and digested by proteinase K ...
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bioRxiv - Plant Biology 2020Quote: The non-polar phase containing non-polar metabolites which included fatty acids was subjected to gas chromatography-flame ionization detection (GC-FID) (Clarus 500, Perkin Elmer). The non-polar phase was dried for fatty acid methyl esterification based on Yuan (2016) ...
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bioRxiv - Neuroscience 2020Quote: ... and incubated 10 minutes at 37 °C by addition of 1 μCi/ml of L-[3,4-3H]-Glutamic acid (Perkin Elmer, NET490) and 10µM L-glutamate (Sigma) ...
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bioRxiv - Immunology 2023Quote: ... cells were isolated ex-vivo and then incubated for six hours with 0.5 μCi 14C-palmitic acid (Perkin Elmer Cat# NEC075H050UC) in the following media ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and the supernatant was counted for [14C]-labeled acid-soluble β-oxidation products using a Tri-Carb 4910TR liquid scintillation counter (PerkinElmer). These [14C]-labeled acid-soluble β-oxidation products are deemed to be mainly ketone bodies and to a lesser extent intermediates of the tricarboxylic acid cycle (Fromenty et al ...
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bioRxiv - Cancer Biology 2024Quote: ... The supernatant was counted for [14C]-labeled acid-soluble β-oxidation products using a Tri-Carb 4910TR liquid scintillation counter (PerkinElmer). Results were normalized to cell number as described for oxygen consumption.
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bioRxiv - Biochemistry 2021Quote: ... were based on an initial output from LigPlot+48 and the chemical reaction pathway (Supplementary Fig. 6) was created in ChemDraw (PerkinElmer Informatics).
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bioRxiv - Cell Biology 2021Quote: ... 500nl of 2 µM siRNAs (20nM final concentration) were distributed on 6 different 384-well imaging plates (CellCarrier Ultra, Perkin Elmer), as described in Table 3 ...
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bioRxiv - Immunology 2021Quote: ... brain and testis tissue samples from hACE2 transgenic mice challenged intranasally with SARS-CoV-2-nLuc (1 x 105 FFU; 6 dpi) were imaged after necropsy using bioluminescence imaging (IVIS, Perkin Elmer), pruned to isolate regions with high nLuc activity and immediately pre-fixed with 3 % glutaraldehyde ...
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bioRxiv - Immunology 2021Quote: M-CSF-dependent mouse bone marrow derived macrophages isolated from the long bones of C57BL/6 mice were cultured for 48 hours on devitalized bovine bone discs (0.2 mm x 6 mm) labelled with fluorescently conjugated inert bisphosphonate (Osteosense, Perkin Elmer, NEV10020EX) either in the absence or in the presence of mature osteoclasts that had been differentiated for 5-days under pro-osteoclastogenic conditions i.e ...
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bioRxiv - Cancer Biology 2021Quote: ... sections were stained for NICD1/KRT14/DNA or NICD1/Ki67/DNA and epithelial thickness measured and cell counting performed using Volocity 6 software (Perkin Elmer). Cell density was measured by counting the number of nuclei identified across the width of the epithelium on high resolution images of cryosections stained with DAPI and keratinocyte markers such as KRT4 and ITGA6 and dividing this number by the measured surface area.
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bioRxiv - Cancer Biology 2021Quote: ... Contiguous areas were analyzed for presence or absence of NOTCH1 or nuclear NICD1 staining and their length along the section measured using Volocity 6 software (Perkin Elmer). For morphological analysis of NICD1 and positive negative areas (Extended data Fig.1 f-h) ...
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bioRxiv - Cancer Biology 2021Quote: ... stained sections were imaged using Leica TCD SP8 confocal microscope at 40x objective as described in ‘confocal microscopy’ and analyzed with Volocity 6 software (Perkin Elmer). For counting Ki67 positive basal cells in the aged tissue (Fig.5d) ...