Labshake search
Citations for PerkinElmer :
1851 - 1900 of 3046 citations for Cortisol ELISA Kit 1 Strip plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Approximately 100 µL of resuspended monolayer squares were then transferred into the wells of black-walled thin-bottom 96-well plates (Greiner µClear or PerkinElmer PhenoPlate) preincubated at 37 °C for 30 minutes with 35 µL of 100% Geltrex solution in each well.
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bioRxiv - Cancer Biology 2023Quote: ... Calcein AM was assessed by fluorescence intensity measurement by well scan from bottom with excitation at 494 nm and emission at 517 nm on Ensight plate reader (Perkinelmer, #HH34000000).
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bioRxiv - Cancer Biology 2023Quote: ... Dissociated cells were resuspended in 7.5 mg/mL BME-2 matrix gel at 2-5×104 cells/mL and seeded in 10 μL droplets to individual wells of 96-well Cell Carrier Ultra plates (Perkin Elmer). Once settled ...
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bioRxiv - Immunology 2023Quote: Vero E6 cells were grown in DMEM supplemented with 10% FBS and seeded into white-walled 96 well plates (PerkinElmer, 6005688) at a density of 20,000 cells per well ...
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bioRxiv - Molecular Biology 2023Quote: ... After 24h the luciferase assay was performed in white microplates and luminescence was measured with the EnVision 2104 Multilable plate reader (Perkin Elmer). GLuc production was measured as a surrogate for IFN-β promoter activation by adding 25 µl coelenterazine-solution (1 µg/ml in H2O ...
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bioRxiv - Neuroscience 2023Quote: ... 50 μl of media from each sample and 50 μl of the enzyme and substrate mix was pipetted in a well of the imaging plate (PerkinElmer, 6055300). The plate was briefly mixed and incubated for 1 h at RT avoiding exposure to light ...
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bioRxiv - Biochemistry 2023Quote: ... Filter-bound [3H]VUF15485 was measured by adding 25 µL Microscint-O per well to the dried GF/C-plate and radioactivity was consecutively quantified using the Wallac Microbeta counter (Perkin Elmer). The sigmoidal dose-dependent displacement curves of [3H]VUF15485 binding by unlabelled ligands were analysed using Graphpad Prism 8 ...
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bioRxiv - Microbiology 2023Quote: ... effector cells were detached by pipetting and 16,000 effector cells/well were reseeded into a 96 well black plate (PerKinElmer, Cat# 6005225), and target cells were reseeded at a density of 1 × 106 cells/2 ml/well in a 6 well plate ...
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bioRxiv - Cancer Biology 2023Quote: A total of 1 × 106 Bmal1::luciferase MAFs or Per2::luciferase PDA cells were seeded per well into 24-well black-sided imaging plates (1450-606, Perkin Elmer). Cells were treated with DMSO ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and 100 nL of compound in DMSO from stock plates were added by pin transfer using a Janus Workstation (PerkinElmer, USA). Streptavidin-coated donor beads (15 µg/ml final concentration ...
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bioRxiv - Genetics 2023Quote: ... was added in unison to the lysates and luminescence activity in counts per second were immediately recorded on a Victor X4 plate reader (Perkin Elmer). Using a multichannel pipet ...
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bioRxiv - Cell Biology 2023Quote: ... dissociated MNs were plated in complete MN media at a density of 50,000 cells/well (unless otherwise indicated) in the inner 60 wells of borate/poly-ornithine/laminin/fibronectin coated 96-well plates (Perkin Elmer). Outer wells were filled with water to avoid evaporation effects ...
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bioRxiv - Biochemistry 2023Quote: ... Each binding reaction was allowed to reach equilibrium at 30 °C for 5 min prior to fluorescence polarization measurements in a Victor X5 Multilabel Plate Reader (Perkin Elmer). Each Kd measurement was calculated according to our previous work (45) ...
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bioRxiv - Biochemistry 2023Quote: ... Reactions are incubated at 30 °C for 20 min prior to luminescence measurement for 10 s on the Victor X5 Multilabel Plate Reader (Perkin Elmer) by addition of 75 μl Renilla luciferase substrate (Promega) ...
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bioRxiv - Neuroscience 2023Quote: ... and the emitted light corresponding to the amount of ATP was measured in a Victor® XLight Multimode Plate Reader (PerkinElmer). For each sample ...
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bioRxiv - Immunology 2024Quote: ... was added and live target cells were quantified according to the manufacturer’s protocol with a Victor Nivo® multimode plate reader (Perkin Elmer). An equal number of the target cells alone was used to denote ‘hundred percent luminescence activity’.
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bioRxiv - Neuroscience 2024Quote: The analysis of the nuclear translocation of endogenous TFEB has been performed as follow: 10k cells per well were plated in 96 well plates (PerkinElmer, 6055302) and transfected in suspension using Lipofectamine LTX (Invitrogen ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... was added to the cell suspension and cells were plated (2,500 cells, 6 μL) in a 384-well white opaque microtiter plate (OptiPlates, Perkin Elmer, 6007299). Cells were incubated with serial dilutions (3 μL ...
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bioRxiv - Molecular Biology 2020Quote: 1 μl ml-1 of 0.1 mCi ml-1 14C glucose (PerkinElmer, Cat# NEC042V250UC, Waltham, MA, USA) was added to differentiation medium 48 h before the end of the experiments ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 µl of 3H-labelled SAM (1 mCi/ml; PerkinElmer) was added to the mixture and then incubated at 30°C for 1 h with gentle shaking ...
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bioRxiv - Microbiology 2020Quote: ... France).The bioluminescence signal of Nluc donor and the YFP acceptor emission were acquired using an EnVision Multimode Plate Reader (Perkin Elmer, USA) sequentially using a low and high band pass filter (460/25nm ...
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bioRxiv - Molecular Biology 2021Quote: ... plates were washed thrice with PBS-T and thrice with PBS and 100 µL of DELFIA Enhancement Solution (Perkin Elmer, 4001-0010) was added to each well ...
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bioRxiv - Molecular Biology 2021Quote: ... The plates were read at a wavelength of 460 nm using the VICTOR 3 V 1420 Multi-Label Microplate Reader (Perkin Elmer, USA). All reactions were carried out in triplicate using both antigenic peptides in separate experiments ...
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bioRxiv - Immunology 2021Quote: ... 50μl supernatant was harvested from each well and placed in 96-well Luma Plates containing a dry scintillant (Perkin Elmer, Waltham, MA). Dried plates were counted on a MicroBeta counter (Perkin Elmer ...
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bioRxiv - Cell Biology 2019Quote: ... The library was diluted to a 5 μM stock with 1x siRNA buffer (GE Dharmacon, B-002000-UB-100) and arrayed onto black 384-well PerkinElmer Cell Carrier plates (Perkin Elmer, 6007550) using the Echo Liquid Handler (Labcyte) ...
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bioRxiv - Bioengineering 2020Quote: A spheroid of 4434 BRAF mutant mouse melanoma cells embedded in Matrigel in one well of a plastic bottomed 96-well plate (PerkinElmer, CellCarrier, #6005550) was imaged with the dOPM system ...
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bioRxiv - Cell Biology 2021Quote: ... The screening was performed at same early cell passages for both replicate experiments according to the optimized amount of cells seeded in 384-well plates (ViewPlate-384 Black Perkin Elmer, #6007460) using a Multidrop Combi (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... 15 ul probe mix was added to the corresponding wells of 384-well plate prepared using a JANUS liquid handler workstation (PerkinElmer, Waltham, MA). Probes were denatured at 85°C for 7 min ...
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bioRxiv - Neuroscience 2020Quote: ... Donor fluorescence was measured at 615 nm and acceptor fluorescence at 665 nm with EnVision 2105 multimode plate reader unit (PerkinElmer, Waltham, USA). For quantification ...
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bioRxiv - Microbiology 2021Quote: ... 50 μl of the concentrated bacterial culture was added to two wells of a vitronectin-coated Opera CellCarrier Ultra-96 plate (Perkin Elmer, 6055302), and the plates were incubated static at 37 °C for 10 min ...
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bioRxiv - Microbiology 2021Quote: ... 200 μL of each mixed culture was aliquoted into a 96-well plate and GFP fluorescence and optical density (at 600 nm) was estimated in a multi-mode plate reader (Varioskan-Thermo Scientific or Enlight-Perkin Elmer). For each individual experiment 2 controls were maintained ...
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bioRxiv - Cell Biology 2021Quote: ... The ATPLite™ assay followed standard recommended no wash one step protocol and was read on an EnSight™ Multimode Plate Reader (PerkinElmer), without any deviation from the manufacturers protocol ...
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bioRxiv - Cell Biology 2021Quote: ... after which Stop & Glo was added to measure renilla luciferase activity (R-luc) on an EnSpire Multimode Plate Reader (Perkin Elmer, USA). F-luc was normalised to R-luc to account for transfection efficiency ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Total cell counting assays were performed by seeding MDA-MB-231 cells (WT and ClpP-KO; 4000 cells/well) or SUM159 (WT and ClpP-KO; 1000 cells/well) in a 96-well plate (Perkin Elmer, 6005050) and allowed to adhere overnight ...
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bioRxiv - Neuroscience 2020Quote: ... and old (25-day-old) flies were placed individually in every other well of a 96-well white microtiter plate (Perkin Elmer, USA) in which each well contained 200 µl of a 5 % sucrose ...
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bioRxiv - Biochemistry 2020Quote: ... 20 μL of lysate was added in triplicate to a 384-well white-walled plate (ProxiPlate-384 Plus, shallow-well microplate, PerkinElmer, Waltham, MA) and read on an EnVision plate reader (PerkinElmer ...
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bioRxiv - Cancer Biology 2022Quote: ... and luminescence was measured on a BioTek plate reader using the Britelite Plus Reporter Gene Assay System (Perkin Elmer 6066761, Waltham, MA). Each assay included triplicates of the treatment conditions as well as a no drug control and a B16F10 cell only control ...
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bioRxiv - Cancer Biology 2021Quote: ... Plates sat at room temperature for an additional 10 min in the dark before samples were read on an EnVision XCite plate reader (PerkinElmer # 2105-0020).
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bioRxiv - Microbiology 2019Quote: ... Nluc activity was measured from cell lysates within 10 min of lysis (or substrate addition) by transferring lysate into white OptiPlate 96-well plates (PerkinElmer, Waltham, MA) and measured in a Berthold Centro XS3 LB 960 luminescent plate reader with readings integrated over 0.2 sec.
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bioRxiv - Microbiology 2020Quote: ... Plates were incubated for >30 min at room-temperature to allow the reaction to reach equilibrium and read using an EnVision plate reader (PerkinElmer, Waltham, Massachusetts). Luminescence from uninfected wells was determined for each treatment and subtracted from infected wells to account for signal not derived from parasites.
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bioRxiv - Microbiology 2020Quote: The sensor cells were seeded on the day of the experiment into tissue culture-treated polystyrene 96-well plates with light-tight walls and translucent bottom (ViewPlate-96, PerkinElmer, Cat#6005181) as 60,000 cells per well in 180 μl of complete medium ...
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bioRxiv - Synthetic Biology 2021Quote: ... The colonies were grown in 160 μL of TAP medium for 5 days in black clear bottom 96 well plates (CellCarrier-96 Black, 6005550, PerkinElmer Inc, USA), sealed with a paper tape ...
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bioRxiv - Immunology 2021Quote: ... Separated lipoproteins were quantitated and results are presented as optical density (OD) measurements analyzing the absorbance at 492 nm using a plate reader (Perkin Elmer Enspire).
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bioRxiv - Cell Biology 2020Quote: ... The resultant formazan product was dissolved in DMSO (200 μl/well) and absorbance was measured at 492 nm by EnVision Multimode Plate Reader (Perkin Elmer, USA).
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bioRxiv - Cell Biology 2020Quote: ... The alpha-glucosidase activity was determined by measuring the yellow-colored para-nitrophenol released from pNPG at 405 nm (EnVision Multimode plate reader, Perkin Elmer, USA). The results were expressed as the percentage of the blank.
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bioRxiv - Neuroscience 2020Quote: ... Embryoid body differentiation was initiated on day 0 by detaching 70-80% confluent iPSCs with 0.5 mM EDTA and seeding 9 000 cells/well on ultra-low attachment U-bottom 96-well plates (CellCarrier-96 Spheroid ULA, Perkin Elmer 6055330) in 150μl of E8+0.5% P/S supplemented with 20 μM ROCK inhibitor ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The membrane mixture was then filtered over a 0.3% PEI pre-treated GF-B/C plate (#6005174, Perkin Elmer, Waltham, MA, USA) with a cell harvester system ...
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bioRxiv - Cancer Biology 2019Quote: 1000 U2OS-KDM4B expressing cells in 25 μl of media were plated into each well of a poly-D-lysine coated Perkin Elmer 384-well View plates (Perkin Elmer 6007710) with a Thermo Scientific Wellmate ...
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bioRxiv - Developmental Biology 2022Quote: ... 100 μL of resuspension was added to three wells of a 96-well OptiPlate black plate (Perkin Elmer Life Sciences, Waltham, MA). The emission was then read using the filter 640/680 at a gain of 1000 using a PHERAstar FS microplate reader (BMG LABTECH ...
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bioRxiv - Developmental Biology 2022Quote: ... was used to generate a standard curve and color change was then quantified using the EnSight multimode plate reader (Perkin Elmer, USA) and analyzed using the proprietary software ...