Labshake search
Citations for PerkinElmer :
1701 - 1750 of 3095 citations for Cortisone Chemiluminescent ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2021Quote: ... was added to each well and the luminescence of the firefly luciferase was measured using EnVision plate reader (Perkin Elmer) after 20 min incubation at RT ...
-
bioRxiv - Cell Biology 2021Quote: ... Cells were washed twice with D-PBS and then resuspended in D-PBS before being seeded on 384-well plates with poly-D-lysine coating (PerkinElmer CellCarrier Ultra 384 ...
-
bioRxiv - Neuroscience 2021Quote: ... and seeded at 0.6 × 106 cells/ml on a poly-lysine/ laminin substrate in a 96 well plate (PerkinElmer #6005558). Primary cortical neurons were cultured for 5 days and then transfected using Lipofectamine 2000 (ThermoFisher # 11668019) ...
-
bioRxiv - Neuroscience 2020Quote: ... cells were plated in a 96-well plate (40,000 cells/well) and were lysed using mammalian lysis cell buffer (Perkin Elmer) and then shaken for 5 min in an orbital shaker at 700 rpm ...
-
bioRxiv - Cancer Biology 2021Quote: ... Calcein-AM fuorescence of EC-adhered Jurkat cells was immediately read on a fluorescence plate reader (Perkin Elmer EnSpire 2300) at 495/515 nm ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... The fluorescence signal was measured using an excitation wavelength of 360 nm and emission wavelength of 460 nm on an EnVision Multilabel plate reader (PerkinElmer). The SIRT-1 reactions were carried out in a 15 µL mixture containing 50 mM Tris-HCl (pH 8.0) ...
-
bioRxiv - Genetics 2022Quote: ... 7.5×104 cells in supplemented McCoy’s 5A media −/+ 500 µM auxin were seeded in 384-well plates (Perkin Elmer 6057300) and incubated at 37°C for 6 h ...
-
bioRxiv - Synthetic Biology 2022Quote: ... were cultured and transfected as described previously53 with the following modifications: transfections were carried out in suspension using 24 well plates (either black for light experiments, PerkinElmer, or transparent for dark control ...
-
bioRxiv - Microbiology 2019Quote: ... were infected with viral particles of Makona GP-A82 or V82 pseudoviruses in a 48 well plate using the same amount of p24 antigen quantified by AlphaLISA (AL291C:500 assay; PerkinElmer). After 48 h of incubation cells were washed twice with PBS and lysed for luciferase assay ...
-
bioRxiv - Synthetic Biology 2019Quote: ... 190 µl of each strain and of the media was placed in wells of a 96-well plate (Perkin Elmer). The negative feedback strain was also placed in other wells where following final concentrations of inducer aTc (in µg/ml ...
-
bioRxiv - Synthetic Biology 2020Quote: ... The sample plate was incubated for 30 min at room temperature in darkness and read on a Victor3 luminometer (PerkinElmer).
-
bioRxiv - Cell Biology 2019Quote: GBM2 cells were plated at 2000 cells/well and exposed to Prestwick compounds (3 µM; Table 6) for 3 days in 384-well optical bottom assay plates (PerkinElmer). Cells were then fixed and stained with rabbit polyclonal anti-H3K27ac and mouse monoclonal anti-H3K27me3 antibodies followed by AlexaFluor-488- or AlexaFluor-555-conjugated secondary antibodies ...
-
bioRxiv - Neuroscience 2019Quote: ... were transfected into the indicated cells (HEK293/17: 4×105/well; CHO: 2.5×104/well, BMVECs: 5×103/well) in 96-well plates (PerkinElmer, 6005680) in triplicate ...
-
bioRxiv - Bioengineering 2020Quote: Bioprinted SK-N-BE(2) spheroids were prepared as described earlier in a 96-well plate (Cell-Carrier, Perkin Elmer). After incubating the plate for 3 days ...
-
bioRxiv - Molecular Biology 2021Quote: ... Plates were incubated at RT for 40 min and the fluorescence intensity measured at 620 nm and 650 nm by an EnVision multilabel plate reader (PerkinElmer).
-
bioRxiv - Molecular Biology 2020Quote: ... The signals of time-resolved fluorescence resonance energy transfer (TR-FRET) assay were measured in an Envision plate reader (PerkinElmer) with a TR-FRET mode (Ex=320 ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... Optical density at 600 nm (OD600) was measured using a microplate reader (Victor multilabel Plate Reader, Perkin Elmer, United States).
-
bioRxiv - Molecular Biology 2021Quote: ... were added and the plates were subsequently counted for radioactivity (counts per minute, CPM) in a MicroBeta2 microplate counter (PerkinElmer).
-
bioRxiv - Cell Biology 2019Quote: ... Assay ready plates were prepared by first spotting 2 μl of a 400 nM solution of siRNA oligos at the bottom of a dry 384-well plate using the MDT module of a Janus automated liquid handler (PerkinElmer), and then air-drying under a laminar sterile air-flow for 2 hrs ...
-
Nonsense Mediated RNA Decay Is a Unique Vulnerability of Cancer Cells with SF3B1 and U2AF1 MutationsbioRxiv - Cell Biology 2021Quote: ... Interaction of donor and acceptor beads generates a luminescent signal that is measured using an EnVision plate reader (Perkin Elmer) with 680 nm excitation and 520-620 nm emission.
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... Emission intensity was recorded for 5 min using 460 nm and 530 nm filters on the EnVision or Victor X Light plate reader (PerkinElmer). Next ...
-
bioRxiv - Neuroscience 2021Quote: ... after 1 hour at 37°C the reaction was stopped with 0.25 M glycine buffer pH 10.4 and the fluorescence emission of the 4-methylumbelliferyl generated (4-MU) by the reaction was read with a fluorimeter (EnSpire Plate Reader, PerkinElmer) selecting an excitation wavelength of 365 nm and an emission wavelength of 445 nm ...
-
bioRxiv - Cancer Biology 2021Quote: ... At the end of the incubation period the uptake of the reporter was analyzed by fluorescence microscopy or measuring bioluminescence signals after adding furimazine (Fz; 25 μM) using a VICTOR Nivo Multimode Plate Reader or IVIS Lumina (PerkinElmer).
-
bioRxiv - Molecular Biology 2021Quote: ... Plates were incubated in the dark for 2 h at room temperature before measuring fluorescence using the EnVision Multimode Plate Reader (PerkinElmer), as described previously74 ...
-
bioRxiv - Microbiology 2020Quote: ... The supernatant (70 μls) was transferred to a microtiter plate and the luminescence was read in an Envision 2103 microplate luminometer (PerkinElmer). Alternatively ...
-
bioRxiv - Microbiology 2021Quote: ... duplicate twofold serial dilutions of various compounds were prepared in assay medium in sterile 96-well black opaque plates (CulturPlate-96 Black, PerkinElmer). To allow for synergy testing ...
-
bioRxiv - Microbiology 2021Quote: ... TZM-bl reporter cells were seeded at a density of 2 × 104 cells/well in 96-well luminometer-compatible tissue culture plates (PerkinElmer) 24 h before infection ...
-
bioRxiv - Immunology 2020Quote: ... luciferase activities of 150 μl lysate transferred from each well to a 96-well black solid plate were measured by a luminometer (PerkinElmer). The 50% inhibitory dose (ID50 ...
-
bioRxiv - Immunology 2020Quote: ... Reactions were incubated for 30 minutes and FP was measured on a Perkin Elmer Envision multi-mode plate reader (PerkinElmer), by exciting the FAM fluorophore of GSK306 at a wavelength of 485 nm and then measuring emission at 535 nm in both parallel and perpendicular planes ...
-
bioRxiv - Immunology 2021Quote: ... cell lysate was loaded onto a 96-well plate (Grenier-BioOne) and mixed with MICROSCINTTM-20 scintillation fluid (Perkin Elmer). Samples were then incubated at room temperature for 2 hours and incorporated radioactivity analyzed on a 1450 MicroBeta TriLux Microplate Scintillation and Luminescence counter (Perkin Elmer).
-
bioRxiv - Immunology 2022Quote: ... Lysis was transferred to chemiluminescence detection plate and the luminescence value was detected with a microplate spectrophotometer (PerkinElmer, Ensight, 6005290). IC50 was determined by a four-parameter logistic regression model.
-
bioRxiv - Genomics 2022Quote: S2R+ cells transfected with Sloth1-FLAG or Sloth2-FLAG were plated into wells of a glass-bottom 384 well plate (6007558, PerkinElmer) and allowed to adhere for 2 hours ...
-
bioRxiv - Cell Biology 2022Quote: ... Malondialdehyde (MDA) levels were obtained by detecting fluorescence at 530 nm / 550 nm in an EnVision 2104 Multilabel plate reader (PerkinElmer).
-
bioRxiv - Biochemistry 2022Quote: ... Enzyme is incubated with 25 μM compound or 1.25% DMSO in 384-well plates (Perkin Elmer Proxiplate 384F Plus #6008269) for 30 min before the addition of an equal volume of substrate (800 nM final concentration ...
-
bioRxiv - Neuroscience 2022Quote: ... Drugs were added to the plates using a 50 nL solid pin tool attached to a Janus automated workstation (PerkinElmer), for a final screening concentration of 100 nM ...
-
bioRxiv - Microbiology 2022Quote: ... OD600 and luminescence measurements were taken either 1-hour post-resuspension or over 6 hours starting at 0.5-hour post-resuspension using the Victor X3 2030 multilabel plate reader (Perkin Elmer). Luminescence was measured in counts per second (CPS ...
-
bioRxiv - Systems Biology 2022Quote: ... All ELISA and colorimetric assays were performed according to manufacturer’s instructions in combination with a microplate reader (VICTOR3 Multilabel Plate Reader, Perkin Elmer).
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... stained with Hoechst 33342 and plates were imaged on a High-Content Imaging device (Operetta HCS epifluorescence microscope, Perkin Elmer). Three fields per well were acquired at 10x magnification in blue channel (excitation ...
-
bioRxiv - Plant Biology 2022Quote: ... and the samples were pipetted in three technical replicates into a 384 well plate using an automated JANUS Workstation (PerkinElmer). According to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2023Quote: ... The tissue lysates were mixed with an equal volume of Nano-GLO Luciferase Assay Reagent (Nanolight) and luciferase activity was quantified on an Envision 2103 plate reader (PerkinElmer). Decoy concentration and half-life were determined based on a standard curve derived from the mixture of serial diluted ACE2.1mb-nLuc proteins and tissue lysate from wild-type ...
-
bioRxiv - Molecular Biology 2023Quote: ... Crystal Violet was re-solubilized in methanol and O.D.595 was measured in the VICTOR X3 Multilabel Plate Reader 2030-0030 (Perkin Elmer). GraphPad was used for statistical analysis and the value of untreated cells was set at 100% survival.
-
bioRxiv - Immunology 2023Quote: ... Signals of time resolved-fluorescence resonance energy transfer (TR-FRET) were collected by an EnVision 2105 multimode plate reader (PerkinElmer). Intracellular cAMP levels were calculated as instructed by the manufacturer.
-
bioRxiv - Immunology 2024Quote: ... the plates were centrifuged at 500 × g for 5 min and 20 µl of supernatant from each well was transferred to a 96 well DELFIA Yellow Plate (PerkinElmer) and combined with 200 µl of europium ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... The reaction was stopped with Stop Solution and the absorbance was read at 450 nm using a VICTOR 1420 Multilabel plate reader (PerkinElmer). Using the absorbance of the standard ...
-
bioRxiv - Cell Biology 2024Quote: ... HeLa mt-AEQ cells infected with lentivirus carrying the specific shRNA were seeded in white 96-well plates (PerkinElmer, 6005181) at 25000 cells/well ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... The plates were further incubated for 20 min at room temperature and counted using the Wallac TriLux Microbeta counter (PerkinElmer). The results were analysed using GraphPad Prism 9.1 ...
-
bioRxiv - Cancer Biology 2024Quote: ... Change in initial ATP concentration was measured as bioluminescence readout in counts per second on the Envision plate reader (PerkinElmer).
-
bioRxiv - Cancer Biology 2024Quote: ... by measuring bottom-read fluorescence at 520 nm with excitation wavelength set at 480 nm using EnSight Multimode Plate Reader (PerkinElmer). The same protocol was adapted to measure the effect of paracrine signaling on the proliferation rates of parental PNT1A (1.0 × 103 cells/well) ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... signals of time resolved-fluorescence resonance energy transfer (TR-FRET) were detected by an EnVision 2105 multimode plate reader (PerkinElmer). Intracellular cAMP levels were calculated according to the TR-FRET signals of the samples and cAMP standards.
-
bioRxiv - Biochemistry 2024Quote: ... signals from time-resolved fluorescence resonance energy transfer (TR-FRET) were recorded using an EnVision 2105 multimode plate reader (PerkinElmer). The determination of intracellular cAMP levels was based on the TR-FRET signals of the samples in comparison to cAMP standards.