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Citations for PerkinElmer :
1601 - 1650 of 2892 citations for Cortisone ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... and the samples were pipetted in three technical replicates into a 384 well plate using an automated JANUS Workstation (PerkinElmer). According to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... The tissue lysates were mixed with an equal volume of Nano-GLO Luciferase Assay Reagent (Nanolight) and luciferase activity was quantified on an Envision 2103 plate reader (PerkinElmer). Decoy concentration and half-life were determined based on a standard curve derived from the mixture of serial diluted ACE2.1mb-nLuc proteins and tissue lysate from wild-type ...
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bioRxiv - Molecular Biology 2023Quote: ... Crystal Violet was re-solubilized in methanol and O.D.595 was measured in the VICTOR X3 Multilabel Plate Reader 2030-0030 (Perkin Elmer). GraphPad was used for statistical analysis and the value of untreated cells was set at 100% survival.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The reaction was stopped with Stop Solution and the absorbance was read at 450 nm using a VICTOR 1420 Multilabel plate reader (PerkinElmer). Using the absorbance of the standard ...
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bioRxiv - Microbiology 2023Quote: ... 100 μl of each sample was used to measure the optical density at 600 nm (OD600) using an EnVision 2105 Multimode Plate Reader (PerkinElmer). 93 μl of each sample were loaded into a black ...
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bioRxiv - Microbiology 2023Quote: ... cells were cultured in 6-well plates and metabolically labeled (Easytag Express 35S-Met-Cys protein labeling mix (Perkin Elmer)) with 0.2 mCi/ml for various times ...
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bioRxiv - Microbiology 2023Quote: ... A total well volume of approximately 112.5 μL was incubated at room temperature for 2 minutes prior to measuring luminescence and OD600 readings on an EnVision plate reader (PerkinElmer).
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bioRxiv - Cell Biology 2023Quote: HeLa cells (9000 cells per 100 ml of culture medium) were seeded in each well of a 96-well plate CellCarrier-96 Ultra Microplates (6055302, PerkinElmer). Cells were cotransfected with 100 ng each of individual GFP-Rabs and mito-mScarlet-DENND6A or mito-mScarlet using jetPRIME ...
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bioRxiv - Microbiology 2023Quote: ... before removing the supernatant underneath the pellicle and scanning the plate in an Opera QEHS high-content screening microscope (PerkinElmer) equipped with a UAPO20×W3/340 objective with NA = 0.7.
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bioRxiv - Immunology 2023Quote: ... 50 μl of stop solution (0.3 N H2SO4) was added and the absorbance read at 450 nm on an Enspire multimode plate reader (Perkin Elmer). Background signals from control wells incubated with bNAbs and then conjugate were subtracted ...
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bioRxiv - Cell Biology 2023Quote: ... Plates were thawed the following day at room temperature and well contents were collected onto filtermats using a plate harvester (PerkinElmer). A Micro Beta liquid scintillation counter (PerkinElmer ...
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bioRxiv - Cell Biology 2023Quote: ... On the next day transfer and mix of compounds into plates only containing cell culture media was performed with the Sciclone G3 Liquid Handling Workstation (PerkinElmer). This intermediate dilution step was included to avoid DMSO gradient effects on cell monolayers ...
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bioRxiv - Systems Biology 2022Quote: ... 100 μl were transferred to a white 96-well microplate and luminescence was measured using on an EnVision plate reader (PerkinElmer).
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bioRxiv - Systems Biology 2023Quote: ... A breathable membrane was applied over the plate and cells were imaged every 10 min on the Operetta CLS high-content microscope (PerkinElmer) at 37°C and 5% CO2 for 72h using the 20x N.A ...
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bioRxiv - Biochemistry 2023Quote: ... Plates were incubated at RT for 15min and the fluorescence intensity measured at 620 nm and 665 nm by an EnVision multilabel plate reader (PerkinElmer).
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bioRxiv - Neuroscience 2023Quote: ... Three organoids per cell line and per condition were transferred each in one well of the imaging plate (PerkinElmer, 6055300). 50 µl of CellTiter-Glo® reagent were added to each well and the plate was incubated for 30 min on a shaker at room temperature (RT) ...
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bioRxiv - Cancer Biology 2023Quote: Imaging of the 384 well plates was performed with an Opera Phenix automated spinning-disk confocal microscope (Perkin Elmer, HH14000000) at 20x magnification for all drug screening assays with the exception of 3D glioblastoma cell lines (ZH-161 ...
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bioRxiv - Microbiology 2023Quote: ... Lipid peroxidation was assessed by measuring the change in fluorescence of C11-BODIPY at 488nm using direct fluorescence reading with the plate reader (Envision PerkinElmer).
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bioRxiv - Bioengineering 2023Quote: ... overexpressing the human FGFR1c together with β-klotho was plated into 384-well micro-titer plates (Perkin Elmer, Cat. #6007480) and incubated at 37°C ...
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bioRxiv - Biochemistry 2023Quote: ... Transfected cells were trypsinized and 0.02 x 106 cells were plated per well in CellCarrier 96 well plates (Perkin Elmer). The next day ...
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bioRxiv - Molecular Biology 2023Quote: ... HCT116 degron cells transduced with HART-ODC1 or HART-RAC1 were plated in a 384 well plate (Perkin Elmer #6057302) and were treated with AUX or DMSO for 96 hours ...
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bioRxiv - Immunology 2024Quote: ... with fluorescence detected on intensity (485 nm excitation and 530 nm emission) measured by an EnSpire Alpha Multimode Plate Reader (PerkinElmer).
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bioRxiv - Immunology 2024Quote: ... the plates were centrifuged at 500 × g for 5 min and 20 µl of supernatant from each well was transferred to a 96 well DELFIA Yellow Plate (PerkinElmer) and combined with 200 µl of europium ...
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bioRxiv - Neuroscience 2023Quote: ... The fluorescence signal at an excitation/emission of 570/610 nm was measured in end-point mode using the EnVision 2104 plate reader (PerkinElmer). A resorufin reference fluorescence standard curve was used to determine the amount of reaction product in each well from the measured relative fluorescence units (RFU) ...
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bioRxiv - Cancer Biology 2024Quote: ... was added and cells were incubated for 90 minutes at 37°C before measuring fluorescence using an EnVision plate reader (PerkinElmer). For longer-term proliferation assays ...
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bioRxiv - Microbiology 2023Quote: ... ε-NAD was used as a fluorogenic substrate to report ThsA enzyme NADase activity by monitoring increase in fluorescence (excitation 300 nm, emission 410 nm) using EnSpire Multimode Plate Reader (PerkinElmer) at 37 °C ...
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bioRxiv - Cell Biology 2024Quote: ... HeLa mt-AEQ cells infected with lentivirus carrying the specific shRNA were seeded in white 96-well plates (PerkinElmer, 6005181) at 25000 cells/well ...
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bioRxiv - Immunology 2023Quote: ... Signals of time resolved-fluorescence resonance energy transfer (TR-FRET) were collected by an EnVision 2105 multimode plate reader (PerkinElmer). Intracellular cAMP levels were calculated as instructed by the manufacturer.
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bioRxiv - Biochemistry 2024Quote: ... signals from time-resolved fluorescence resonance energy transfer (TR-FRET) were recorded using an EnVision 2105 multimode plate reader (PerkinElmer). The determination of intracellular cAMP levels was based on the TR-FRET signals of the samples in comparison to cAMP standards.
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bioRxiv - Biochemistry 2024Quote: ... 10 µl of pre-made compound solution in media was added to each well and luciferase measurements were performed on an EnVision plate reader (PerkinElmer).
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bioRxiv - Biochemistry 2024Quote: HeLa cells stably expressing GFP-LC3 were plated in 96- or 384-well plates (Perkin Elmer Cell Carrier Ultra; 6057308) at 10000 or 3000 cells per well in supplemented DMEM ...
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bioRxiv - Cancer Biology 2024Quote: ... by measuring bottom-read fluorescence at 520 nm with excitation wavelength set at 480 nm using EnSight Multimode Plate Reader (PerkinElmer). The same protocol was adapted to measure the effect of paracrine signaling on the proliferation rates of parental PNT1A (1.0 × 103 cells/well) ...
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bioRxiv - Biochemistry 2024Quote: ... Master mixes of each reaction incubated for 30min at room temp in the dark before being loaded into 384 well flat black plate (PerkinElmer) and scanning each well 20 times on a Tecan Spark plate reader at 488 nm excitation and 530 nm emission ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... signals of time resolved-fluorescence resonance energy transfer (TR-FRET) were detected by an EnVision 2105 multimode plate reader (PerkinElmer). Intracellular cAMP levels were calculated according to the TR-FRET signals of the samples and cAMP standards.
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The plates were further incubated for 20 min at room temperature and counted using the Wallac TriLux Microbeta counter (PerkinElmer). The results were analysed using GraphPad Prism 9.1 ...
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bioRxiv - Cancer Biology 2024Quote: ... Change in initial ATP concentration was measured as bioluminescence readout in counts per second on the Envision plate reader (PerkinElmer).
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bioRxiv - Systems Biology 2021Quote: ... Imaging was performed by HRP luminescence using Femto kit (ThermoFischer Scientific) or Western Lightning PlusECL kit (Perkin Elmer).
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bioRxiv - Microbiology 2020Quote: ... Further 160μl of DNS solution was added in each well and incubated at 100°C for 20 min followed by reading at 570nm in multimode plate reader (Perkin Elmer). All the quantitative assays were done in triplicates and the activity was estimated using the standard graph of glucose.
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bioRxiv - Microbiology 2020Quote: ... The treated plate was then incubated for 1 hour at 37°C with 5% CO2 before measuring the fluorescence at an excitation wavelength of 560 nm and an emission wavelength of 590 nm on a Victor X5 multilabel plate reader (Perkin Elmer).
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bioRxiv - Cell Biology 2020Quote: ... Lysis and luciferase activity detection was performed using the Gaussia-Juice Luciferase Assay (PJK Biotech, Kleinblittersdorf, Germany) according to the manufacturer’s description in white opaque 96-well plates (OptiPlate, PerkinElmer, Waltham, MA) and using a Synergy Mx microplate reader (BioTek ...
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bioRxiv - Developmental Biology 2021Quote: ... 10-20 gastruloids per condition were then transferred into a well of a flat-bottom CellCarrier-96 ultra plate (PerkinElmer, 6007008) and imaged on an Opera Phenix HCS system (PerkinElmer ...
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bioRxiv - Bioengineering 2022Quote: Saturation assays for binding of K1K1H6 to recombinant MET-Fc protein were performed in 384-well microtiter plates (OptiPlate™-384, PerkinElmer© ...
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bioRxiv - Molecular Biology 2021Quote: ... the baseline BRET signals were read immediately at 470 nm and 535 nm for 10 cycles using an EnVision® multimode plate reader (PerkinElmer). Following the agonist addition ...
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bioRxiv - Molecular Biology 2021Quote: ... Plates were read at 450 nm and readings were processed to observe binding patterns of tested molecules (Perkin Elmer, Enspire Alpha). For every experiment ...
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bioRxiv - Cell Biology 2019Quote: ... AMC fluorescence released by cleavage of substrate by proteolytic activity was recorded in a black 96-well plate after 60 min incubation at 37°C using the Victor 3V Multilabel counter (Perkin Elmer). Specific proteasomal activity was determined by subtracting unspecific AMC fluorescence obtained in the presence of proteasome inhibitor MG132 (20 μM) ...
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bioRxiv - Cell Biology 2019Quote: ... 100 µl from each well was transferred to a 96-well plate and the optical density (OD) at 450 nm measured using a Victor X3 microplate reader (Perkin Elmer). Reaction mixture added to a well without cells served as a blank control and was used to subtract the background fluorescence from the samples ...
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bioRxiv - Microbiology 2021Quote: ... 20 mM HEPES (pH 7.2 were seeded 48 h before treatment at a density of 15,000 cells per well in 96-well imaging plates (PerkinElmer cat. 6005182). Inhibitors ...
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Dimeric prion protein ligand activates Adgrg6 but does not rescue myelinopathy of PrP-deficient micebioRxiv - Neuroscience 2020Quote: ... The reaction was stopped with 0.5 M H2SO4 and absorbance at 450 nm was measured in a plate reader (Perkin Elmer, EnVision). The experiment was performed with technical duplicates.
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bioRxiv - Neuroscience 2021Quote: ... TR-FRET measurements were read out using previously reported parameters (Ballmer et al., 2017) on an EnVision multimode plate reader (Perkin Elmer).
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bioRxiv - Cell Biology 2021Quote: ... 30 μL of the cell suspension was plated in duplicate into wells of a 384-well white-walled plate (PerkinElmer CulturePlate) and coelenterazine-h was added to a final concentration of 3.375 μM ...