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Citations for PerkinElmer :
1601 - 1650 of 1683 citations for 6 4 Methyl 1 piperazinyl N 5 methyl 1H pyrazol 3 yl 2 1Z 2 phenylethenyl 4 pyrimidinamine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... [methyl-14C] CDP-choline (50–60 mCi/mmol) and [diarachidonyl-1-14C] PC (50–60 mCi/mmol) were purchased from Perkin Elmer, Inc (Waltham ...
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bioRxiv - Immunology 2021Quote: ... 293T-ACE2 target cells were seeded at a density of 1×104 cells/well in 96-well luminometer-compatible tissue culture plates (Perkin Elmer) 24h before infection ...
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bioRxiv - Neuroscience 2021Quote: ... The signal of CCK hybridization was revealed by a TSA reaction using fluorescein isothiocyanate (FITC)-labeled tyramide (1:100 for 10 minutes, NEL741001KT Perkin Elmer). After several washes ...
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bioRxiv - Cancer Biology 2020Quote: ... membranes were washed and probed with horseradish peroxidase (HRP)-conjugated secondary antibodies for 1 hour before detection with Western Lightning Chemiluminescence (Perkin Elmer). Antibodies used included ...
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bioRxiv - Microbiology 2021Quote: ... 293T-ACE2 target cells were seeded at a density of 1×104 cells/well in 96-well luminometer-compatible tissue culture plates (Perkin Elmer) 24h before infection ...
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bioRxiv - Plant Biology 2019Quote: ... triplicate sets of 10 seedling were gently rinsed and placed in a 1.7-mL microfuge tube with 1 mL of scintillation fluid (Perkin Elmer Inc) and radioactivity was quantitated by liquid scintillation counting ...
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bioRxiv - Physiology 2019Quote: ... and stimulated with either GLP-1 or GIP for 30 minutes prior to measurement of cAMP accumulation using a LANCE® Ultra cAMP Detection Kit (PerkinElmer) in accordance with the manufacturer’s instructions and as described previously (61 ...
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bioRxiv - Synthetic Biology 2020Quote: ... Each reaction was diluted in TE and mixed with a PicoGreen working stock (diluted to 1/200× in TE Buffer) to be read with an Enspire plate reader (Perkin Elmer) with 480 nm excitation and 520 nm emission filter ...
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bioRxiv - Immunology 2021Quote: ... target cells were seeded at a density of 1 × 104 cells/well in 96-well luminometer-compatible tissue culture plates (Perkin Elmer) 24 h before infection ...
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bioRxiv - Neuroscience 2020Quote: ... and incubated 10 minutes at 37 °C by addition of 1 μCi/ml of L-[3,4-3H]-Glutamic acid (Perkin Elmer, NET490) and 10µM L-glutamate (Sigma) ...
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bioRxiv - Microbiology 2020Quote: ... 293T-ACE2 target cells were seeded at a density of 1×104 cells/well in 96-well luminometer-compatible tissue culture plates (Perkin Elmer) 24h before infection ...
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bioRxiv - Neuroscience 2019Quote: ... Detection was visualized with tyramide-488 1:50 in supplied amplification buffer from the TSA-Plus in situ kit (Perkin Elmer).
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bioRxiv - Genomics 2021Quote: ... the anti-DIG-HRP antibody was detected with the TSA-Cy3 substrate (1:50, TSATM PLUS Cy3 kit, NEL 745001KT, Perkin Elmer) for 10 min at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were then solubilized with 200μL of 1% SDS 0.2N NaOH and transferred to a scintillation vial with 5mL of Insta-Gel Plus scintillation cocktail (Perkin Elmer #601339). Radioactivity was measured with the TopCount scintillation counter (Perkin Elmer) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Analytical estimation of the stock CoCl2 solution concentration (100 mmol L−1) was performed employing inductively coupled mass spectrometry (Perkin Elmer Avio 200 optical emission spectrometer ...
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bioRxiv - Genetics 2024Quote: ... was done for 1 hour at room temperature and following the addition of the western lightening ECL Pro ECL (Perkin Elmer), images were then directly recorded using Biorad Molecular Imager® Chemi DocTM XRS ...
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bioRxiv - Biochemistry 2024Quote: ... 250 nM purified MBP-Naa80, 200 µM substrate peptide (Figure 1 and Supplemental Table S1, 50 µM 14C-Ac-CoA (Perkin Elmer) were mixed in acetylation buffer (50 mM Tris-HCl pH 8.5 ...
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bioRxiv - Molecular Biology 2024Quote: ... the remaining 20% of the beads (supplemented with 40 µl of 2X ROTI®Load and boiled at 95°C for 10 min) were subjected to SDS-PAGE for immunoblotting together with 1% input samples using HRP-streptavidin conjugate (PerkinElmer; #NEL750001EA) or anti-p65 (Santa Cruz Biotechnology ...
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bioRxiv - Neuroscience 2024Quote: ... washed one time with PBS and one time with PBS+DAPI (1:10 000) and subsequently imaged on the Opera Phenix High Content Screening System (Perkin Elmer) on a 40X water objective ...
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bioRxiv - Bioengineering 2023Quote: UV-vis spectra of the medium and hydrogel samples were measured using a plastic cuvette (1 cm light path) with a UV-visible light spectrophotometer (Lambda 35, Perkin Elmer). The transmittance was measured over the range of 800-400 nm.
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bioRxiv - Cell Biology 2023Quote: ... and 1% antibiotic/antimycotic at 37°C for 30 min and then labeled with Express [35S] Labeling Mix (PerkinElmer, NEG072-007) for 15 min ...
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bioRxiv - Microbiology 2023Quote: ... the labeled sialyl-lactose was eluted with 1 M ammonium formate and quantified by scintillation counting using a Tri-Carb 2810 TR counter (Perkin Elmer).
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bioRxiv - Molecular Biology 2023Quote: ... after which 1 mL of the culture was labelled with 100 DCi/mL of 32P KH2PO4 (Perkin Elmer, 900-1100mCi/mmol) for 6 hours on a shaker at 37 °C ...
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bioRxiv - Cell Biology 2023Quote: ... Fluorescent reporter mVenus and Hoechst were imaged and collected as cycle 1 by Operetta CLS High-Content Analysis System (CLS, Perkin Elmer) using appropriate filters ...
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bioRxiv - Cancer Biology 2023Quote: ... #7074) for 1 hr at room temperature and visualized using Konica Minolta Film Processor with Western Lightning Plus (Perkin Elmer, NEL104001) or Immobilon Crescendo Western HRP substrate (Millipore ...
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bioRxiv - Genomics 2023Quote: ... and streptavidin-conjugated Atto-425 (Bio Trend) at a concentration of 1:400 using TSA-biotin (TSA Plus Biotin Kit, Perkin Elmer). All nuclei were DAPI-stained ...
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bioRxiv - Cancer Biology 2023Quote: ... HRP-coupled anti-mouse-HRP was used (1:2500, Amersham) and detection was done by chemiluminescence (Western Lightning Plus ECL, Perkin Elmer) using the Amersham Imager 600 (GE Healthcare).
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bioRxiv - Biochemistry 2023Quote: ... The amount of glycosylated Rac1 was determined by liquid scintillation counting of each sample for 1 min using a Tri-carb 2910TR scintillation counter (Perkin Elmer). The initial rates of the reaction were fit to the Michaelis Menten equation (eq 1 ...
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bioRxiv - Molecular Biology 2023Quote: ... were cultured at 6000 cells per well in DMEM (+10% FBS, +1% P/S) on Perkin Elmer LLC CellCarrier-96 Ultra Microplates (Perkin Elmer) coated with PLO overnight ...
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bioRxiv - Immunology 2023Quote: ... was injected intraperitoneally per mouse 15 minutes prior to imaging for 1 minute exposure on In Vivo Imaging Spectrum Bioluminescent and Fluorescent Imaging Systems (IVIS) (Perkin Elmer) to confirm infection ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were pulse labeled for 1 hr with 120 μCi of EasyTag Express35S Protein Labeling Mix [35S]-Cys/Met (PerkinElmer; Waltham, MA). Immediately after the radioactive pulse ...
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bioRxiv - Developmental Biology 2021Quote: ... M13mp18 ssDNA was added as a template for DNA replication at the indicated concentrations in presence of α-;32PdCTP (3000Ci mmol−1, Perkin Elmer). At the indicated time points half of the samples were neutralized in 10 mM EDTA ...
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bioRxiv - Cancer Biology 2020Quote: ... proteins were first labeled for 48 h with 0.25 μM Ci ml-1 L-[U-14C] VALINE (Perkin Elmer, Waltham, MA, USA) in GIBCO-RPMI 1640 medium (Invitrogen ...
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bioRxiv - Developmental Biology 2022Quote: ... Tyramide signal amplification reaction was performed using Cy3 fluorescent dye (diluted 1:50 using amplification diluent) (Perkin Elmer Life Sciences, Inc., NEL753001kt). The embryos were co-stained with CrebA and Crb antibodies for visualizing SG nuclei and apical cell boundaries ...
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bioRxiv - Neuroscience 2021Quote: ... sections were incubated in channel 1-specific HRP (323104) for 15min and Cy3-TSAP (1.5K, tyramine signal amplification plus, NEL744E001KT; PerkinElmer Waltham, MA, USA) for 30 min followed by a 15 min incubation in HRP blocker (323107) ...
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bioRxiv - Cell Biology 2021Quote: ... media was removed and replaced with serum-free Modified Eagles Medium (MEM; Mediatech) containing 1 µCi/ml of 3H-myo-inositol (PerkinElmer Life Sciences). Forty-eight hrs post-infection ...
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bioRxiv - Biochemistry 2019Quote: ... the reaction mixtures in Flash plates were incubated for 1 hour and the CPM were measured using Topcount plate reader (Perkin Elmer, www.perkinelmer.com). The CPM counts in the absence of compound for each data set were defined as 100% activity ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Lipodisqs labelled with Cy5 either bound to the polymer SMA or to the lipid DLPE (Supplementary Table 1) were used to study the biodistribution in healthy mice using an IVIS® Spectrum in vivo imaging system (Perkin Elmer). Mice were intravenously injected with these two formulations of Lipodisqs (injected volume of 100 μl with a dose of 100 mg/kg lipid ...
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bioRxiv - Pathology 2021Quote: ... incubated for 1 hour and imaged for brightfield and Hoechst staining using the Operetta CLS™ High-Content Analysis System (PerkinElmer, Inc.). Stained nuclei were counted using the Harmony® software (PerkinElmer ...
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bioRxiv - Neuroscience 2019Quote: ... The secondary and Opal™ steps were then repeated using the anti-rabbit secondary and Opal™ 650 reagent (1:1000; PerkinElmer). Sections were then washed 3x for 2 min each in TBS-T and counterstained with DAPI to label nuclei.
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bioRxiv - Immunology 2021Quote: ... HIV-1 p24 content (produced by the backbone vector) was quantified using the Alliance p24 ELISA Kit (PerkinElmer Health Sciences, Cat# NEK050B001KT). Relative luminescence units (RLUs ...
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bioRxiv - Bioengineering 2022Quote: ... permeabilized for 30 min in 0.5% Triton X-100 in PBS and stained overnight with 1 μmol/mL CNA35-OG488 [30] and 0.2 nmol/ml OsteoSenseTM 680 (NEV10020EX, PerkinElmer, Waltham, MA, USA) at 4 ºC to visualize collagen and hydroxyapatite ...
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bioRxiv - Biochemistry 2022Quote: ... Detection reagents were added stepwise with 30 min incubation of anti-H3K4me3 antibody and Ulight-Streptavidin with reaction time points followed by 1 hr incubation with Europium anti-rabbit antibody in 384 well white microplates (PerkinElmer OptiPlate-384). TR-FRET emission at 665 nm and at 615 nm by 320 nm excitation with 50 μs delay and 100 μs integration time was measured using a Molecular Devices SpectraMax M5e plate reader ...
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bioRxiv - Microbiology 2023Quote: Prokaryotic (bacteria and archaea) heterotrophic production was estimated using the 3H-leucine incorporation method (Perkin Elmer, specific activity 100 Ci mmol−1). Water samples (1.7 mL each ...
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bioRxiv - Biochemistry 2023Quote: ... Reactions were initiated by adding an equal volume of assay buffer containing 1 μm [γ-32P]GTP (~50 cpm/fmol, Perkin Elmer) at 30 °C ...
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bioRxiv - Genetics 2022Quote: ... The physical titer of vector preparations was measured using the HIV-1 Gag p24 antigen immunocapture assay kit (PerkinElmer, Waltham, MA, USA) and expressed as p24 ng/ml ...
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bioRxiv - Biochemistry 2023Quote: ... BRB80 was mixed with a 1/10 volume of 10X nucleotide exchange buffer (10 mM GTP, 30 nM GTP α-32P (PerkinElmer BLU506H250UC), 1 mM nocodazole ...
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bioRxiv - Cell Biology 2023Quote: ... 5 μl cAMP Eu-cryptate reagent and 5 μl of anti-cAMP-d2 working solution were added to the 384-well plates and incubated for 1 h.54 Fluorescence signals were detected at 620/665 nm using the Multimode Plate Reader (PerkinElmer EnVision 2105).55 Data were analyzed with GraphPad Prism 9.0 ...
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bioRxiv - Molecular Biology 2023Quote: ... GLuc production was measured as a surrogate for IFN-β promoter activation by adding 25 µl coelenterazine-solution (1 µg/ml in H2O) was to 25 µl supernatant.reader (Perkin Elmer, Waltham, USA) and luminescence was measured ...
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bioRxiv - Cancer Biology 2023Quote: ... The amount of eATP was analyzed according to the protocol described by the manufacturer of ATPlite 1-step Luminescence Assay System (PerkinElmer, Cat# 6016736) either at 0 hours (eATP concentrations were determined immediately ...