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Citations for PerkinElmer :
101 - 150 of 437 citations for SARS CoV 2 Spike Glycoprotein S2 Sheep Fc Tag HEK293 Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2019Quote: ... were transfected into the indicated cells (HEK293/17: 4×105/well; CHO: 2.5×104/well, BMVECs: 5×103/well) in 96-well plates (PerkinElmer, 6005680) in triplicate ...
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bioRxiv - Biochemistry 2021Quote: ... microsomes prepared from HEK293 cells expressing RyR2 were incubated for 1 h at 25 °C with 5 nM [3H]ryanodine (PerkinElmer) in reaction media containing 0.17 M NaCl ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Binding experiments were performed on membranes prepared from wild-type and HA-MOP-transfected HEK293 cells using [3H]DAMGO (51.7 Ci/mmol) (PerkinElmer NET902250UC) as described previously52 ...
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bioRxiv - Microbiology 2021Quote: ... Oligonucleotide probes (listed in Supplementary Table S2) were 5′ 32P-end labeled with 0.3 mCi of γ-32P ATP (Perkin Elmer) by incubating with 10 U of T4 polynucleotide kinase (New England Biolabs ...
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bioRxiv - Molecular Biology 2022Quote: ... and a DNA loading control also terminating in 20 adenosines (IDT) (Supplementary Table S2) were 5’ labelled with [γ-32P]-ATP (PerkinElmer) using T4 poly-nucleotide kinase (NEB ...
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bioRxiv - Biophysics 2023Quote: ... LeuT was mixed with Yttrium Silicate Copper (YSi-Cu) His-tag SPA beads (PerkinElmer) and [3H]leucine in binding buffer (200 mM NaCl ...
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bioRxiv - Neuroscience 2022Quote: ... the sections were treated with TSA PLUS Biotin Kit (PerkinElmer Inc., Waltham, MA USA) and the respective mRNAs (5-ht2Cr or Ppg ...
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bioRxiv - Developmental Biology 2019Quote: ... and FOXJ1 were performed using a TSA Biotin Tyramide Amplification system (SAT700001EA, Perkin Elmer) for amplification ...
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bioRxiv - Developmental Biology 2021Quote: ... followed by multi-step detection with the Biotin-Tyramide signal amplification kit (Perkin Elmer) with a 15 minute exposure time ...
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bioRxiv - Bioengineering 2022Quote: ... Two avalanche photodiodes detect the fluorescent photons (SPCM-AQR-14-FC, Perkin Elmer). The images are obtained by scanning a piezo-controlled stage (Max311D ...
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bioRxiv - Cell Biology 2021Quote: ... Imaging involving mNG-Bud8 (Figs. 3 & S2) was performed using a spinning disk confocal microscope (Ultra-VIEW VoX CSU-X1 system; Perkin Elmer-Cetus) equipped with a 100x /1.45 NA Plan-Apochromat Lambda oil immersion objective lens (Nikon) ...
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bioRxiv - Cell Biology 2020Quote: Two days after transfection the Hek293 cells were washed with PBS and seeded into a 96 well black-white iso plate (Perkin Elmer) in pbs containing 5 mM glucose and supplemented with 5 µM of Nanoglo (Promega #N1120 ...
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bioRxiv - Neuroscience 2019Quote: ... the hybridization signal was amplified with a TSA Plus Biotin Kit (Perkin Elmer, Waltham, MA), by diluting the TSA Plus biotin reagent (1:400 ...
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bioRxiv - Neuroscience 2022Quote: ... Signals were revealed with the Tyramide Signal Amplification (TSA plus biotin) kit (PerkinElmer, cat. #NEL749A001KT) at 1:100 concentration ...
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bioRxiv - Molecular Biology 2024Quote: ... approximately 1 μg of plasmid DNA and 20 μCi of Easy-tag 35S-methionine (Perkin Elmer) were mixed with a final volume of 50 μl ...
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bioRxiv - Neuroscience 2021Quote: ... The sections were then incubated with Biotinyl Tyramide Amplification kits (TSA, TSA plus biotin kit, PerkinElmer), following the manufacture’s protocol ...
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bioRxiv - Genetics 2020Quote: ... Genomic DNA was fragmented with a COVARIS S2 and an Illumina-compatible library was prepared with the NEXTflex Bisulfite Library Prep Kit for Illumina Sequencing (Bioo Scientific/PerkinElmer, Austin, Texas, U.S.A). Bisulfite conversion was performed using the EZ DNA Methylation Gold Kit (Zymo Research ...
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bioRxiv - Molecular Biology 2021Quote: ... was captured on anti-mouse IgG (Fc specific) acceptor beads (Perkin Elmer, Cat nr. AL105C). Serial dilutions of antibodies and VHH-Fc (final concentration ranging between 100nM - 0,001 nM ...
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bioRxiv - Cell Biology 2022Quote: ... The fluorescent photons are detected by two avalanche photodiodes (SPCM-AQR-14-FC, Perkin Elmer). The images are obtained by scanning a piezo-controlled stage (Max311D ...
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bioRxiv - Biophysics 2020Quote: ... The fluorescence was directed to a fiber coupled APD (SPCM-AQR-14-FC, Perkin Elmer), which was connected to a time-correlated single photon counting module (SPC830 ...
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bioRxiv - Neuroscience 2023Quote: ... The fluorescent photons are detected by two avalanche photodiodes (SPCM-AQR-14-FC, Perkin Elmer). The images are obtained by scanning a piezo-controlled stage (Max311D ...
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bioRxiv - Cell Biology 2023Quote: ... The fluorescent photons are detected by two avalanche photodiodes (SPCM-AQR-14-FC, Perkin Elmer). The images are obtained by scanning a piezo-controlled stage (Max311D ...
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bioRxiv - Cell Biology 2023Quote: Pulse-chase experiments using 9.8 Mbq per dish of EASY-TAG EXPRESS Protein labeling mix [35S] (PerkinElmer) and subsequent immunoprecipitation using suitable antibodies and protein G or A-coupled Sepharose beads (GE Healthcare ...
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bioRxiv - Neuroscience 2022Quote: ... The fluorescence signal was confocally detected by an avalanche photodiode (APD; SPCM-AQRH-14-FC; PerkinElmer). The spatial resolution of the setup was around 175 nm (in x-y ...
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bioRxiv - Neuroscience 2019Quote: ... The fluorescence signal was confocally detected by an avalanche photodiode (APD; SPCM-AQRH-14-FC; PerkinElmer). The spatial resolution of the setup was around 175 nm (in x-y ...
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bioRxiv - Biophysics 2019Quote: ... pH 7.5/0-800 mM NaCl (equimolar substitution of NaCl with Tris/Mes)/20% glycerol/0.1 mM TCEP/0.1% DDM using 1.25 mg/mL copper His-tag PVT SPA beads (Perkin Elmer). Total binding was corrected for the non-proximity-based signal to determine the specific binding activity of each LeuT variant and data were normalized to the maximal binding observed for LeuT-WT that was assayed in parallel in all individual measurements ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.5 mM DTT, 0.5 % Sarkosyl, 0.4 u/μl RNase inhibitor, 0.025 mM biotin-ATP/CTP/GTP/UTP [Perkin Elmer]). Equal amounts of nuclei extracted from Drosophila S2 cells were used as spike-in material in run-on reactions ...
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bioRxiv - Zoology 2022Quote: ... The horseradish peroxidase-conjugated Avidin-Biotin Complex was visualized using TSA Plus Fluorescein System (PerkinElmer, Waltham, MA, USA) or 3,3-diaminobenzidine (DAB ...
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bioRxiv - Cancer Biology 2023Quote: ... 0.5 mM DTT, 0.5% Sarkosyl, 0.4 U/µl RNase inhibitor, 0.025 mM biotin-ATP/CTP/GTP/UTP [Perkin Elmer]). Total RNA was extracted using Trizol and ethanol precipitation ...
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bioRxiv - Bioengineering 2021Quote: ... we used the TTL signal from an avalanche photo diode (APD, SPCM-AQRH-13-FC, Perkin Elmer). We illuminated the APD with natural light ...
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bioRxiv - Biophysics 2023Quote: ... CFTR variants were pulse-labeled with 0.1-0.2 mCi/ml 35S-methionine and 35S-cysteine (Easy Tag Express, Perkin Elmer) in Cys- and Met-free DMEM (Gibco™ 21013024 ...
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bioRxiv - Microbiology 2019Quote: ... which were incubated with various concentration of synthetic biotinylated TAR-RNA (biotin 5’-dT/GGU CUC UCU GGU UAG ACC AGA UCU GAG CCU GGG AGC UCU CUG GCU AAC UAG GGA ACC/3’-biotin dT) (Integrated DNA Technologies) at 4°C for 3 h in the AlphaLISA buffer (PerkinElmer). Anti-His Donor and Streptavidin Acceptor AlphaLISA beads (PerkinElmer ...
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bioRxiv - Genomics 2021Quote: ... Aliquots of 10E6 nuclei were subjected to 3-minute nuclear run-on reactions in the presence of Biotin-11-CTP (PerkinElmer) and PRO-seq libraries were constructed in duplicate as described (31) ...
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bioRxiv - Genomics 2022Quote: ... PRO-seq libraries for all replicates were then prepared simultaneously by subjecting one aliquot of 1e7 nuclei/sample to 3-minute nuclear run-on reactions in the presence of Biotin-11-CTP (PerkinElmer) following our previously detailed protocol (24) ...
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bioRxiv - Genomics 2022Quote: ... nuclei were extracted from ~2×107 cells and run-on reactions were carried on at 37°C for 3 minutes using 3μl of each 11-biotin-labelled ribonucleotide stocks (Perkin Elmer). Following real time PCR amplification in the presence of EvaGreen dye as above ...
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bioRxiv - Genomics 2023Quote: The nuclear run-on experiments for PRO-seq were performed in triplicate as described in 47 with the use of a mixture of rNTPs and Biotin-11-CTP (PerkinElmer), and a range of 10-20 million isolated nuclei per sample ...
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bioRxiv - Biophysics 2019Quote: AlphaScreen-based peptide recruitments assays were performed using streptavidin coated donor beads and anti-His-tag antibody coated AlphaLisa acceptor beads (Perkin Elmer). The assays were very sensitive to the distance of the N-terminus to the LXXLL NR-box motif and the use of a flexible Ahx linker ...
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bioRxiv - Neuroscience 2022Quote: ... The medium was removed and cells were incubated with 500 nM of SNAP-Red in Tag-Lite Buffer (Perkin Elmer Cisbio) for 1 hr at 37°C ...
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bioRxiv - Genomics 2020Quote: ... 5 mM MgCl2, 1 mM DTT, 300 mM KCl, 40 µM ATP, 40 µM GTP, 40 µM Biotin-11-CTP [Perkin Elmer] ...
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bioRxiv - Biochemistry 2020Quote: ... and imaged onto the active area of two fibre-coupled avalanche photodiode detectors (APDs; Perkin Elmer, SPCM-AQRH-15-FC) using a cubic non-polarizing beam-splitter (Linos ...
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bioRxiv - Biochemistry 2020Quote: ... peptides were tested for their ability to disrupt a second Alphascreen assay in which donor and acceptor beads were reacted with 50 nM concentrations of a peptide consisting of a biotinylation site and a His-tag peptide (PerkinElmer Life Sciences).
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bioRxiv - Biochemistry 2021Quote: Binding of nucleobases was performed by means of the scintillation proximity assay (SPA) using His-tagged PurTCp in conjunction with Copper HIS-Tag YSI beads (Perkin Elmer, RPNQ0096). 250 ng of purified PurTCp were immobilized on 125 μg SPA beads per 100-μL assay in 200 mM Tris/Mes at the indicated pH ...
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bioRxiv - Biophysics 2020Quote: ... a fast and mild filtration step is performed and the tag-PDZ concentrations were measured by capillary electrophoresis instrument (LabChip GXII, PerkinElmer, Massachusets, USA). A detailed protocol of how to run the holdup assay in an automatic way using liquid handling robots can be found in [29] ...
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bioRxiv - Neuroscience 2023Quote: ... brains were incubated during 2 weeks at 37°C in presence of an AT8 antibody that specifically binds hyperphosphorylated tau (pSer202/Thr205/PSer208, produced at Janssen Pharmaceutica) and is directly labelled with a near-infrared fluorescent tag (PerkinElmer VivoTag 680XL). Brain samples were then dehydrated ...
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bioRxiv - Molecular Biology 2019Quote: ... 0.5% Sarkosyl, 2.5 mM MgCl2, 0.5 mM DTT, 0.05#mM biotin-A/C/G/UTP [Perkin Elmer], 0.4 u/μl RNase inhibitor). The total RNA was isolated with Trizol LS (Invitrogen) ...
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bioRxiv - Microbiology 2020Quote: ... FISH detection was performed using anti-biotin antibody (SA-HRP) and a FITC/Cy5 coupled secondary antibody with the TSA Plus system (Perkin Elmer, USA). Coverslips were stained with Hoechst ...
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bioRxiv - Cancer Biology 2020Quote: ... 2’-14C]-cystine (PerkinElmer) for 5 minutes at room temperature ...
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bioRxiv - Bioengineering 2022Quote: Saturation assays for binding of K1K1H6 to recombinant MET-Fc protein were performed in 384-well microtiter plates (OptiPlate™-384, PerkinElmer© ...
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bioRxiv - Biophysics 2019Quote: ... and a 525/50 nm band-pass filter (F37-516, AHF, Germany) and directed to a fibre-coupled avalanche photo-diode (SPCM-AQR-14-FC, Perkin Elmer) connected to a time-correlated single photon counting (TCSPC ...
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bioRxiv - Bioengineering 2020Quote: MTs were transferred with a tip quickly coated in fetal calf serum (FCS) into a CellCarrier-384 Ultra Microplates (6057300, Perkin Elmer). They were then fixed in 4% PFA (P6148 ...